The Experts below are selected from a list of 3 Experts worldwide ranked by ideXlab platform
Jun Yao - One of the best experts on this subject based on the ideXlab platform.
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Purification and Partial Properties of a Superoxide Dismutase from Agave Hybrid No.11648
Journal of the Chinese Chemical Society, 2012Co-Authors: Bing Chen, Liang Chen, Li-jun Gao, Rui Xiao, Pei-lian Liu, Bi-lian Chen, Yun-xia Zhang, Jun YaoAbstract:A superoxide dismutase enzyme was extracted from the leaves of Agave Hybrid No.11648 using homogenisation, ammonium sulphate fractional precipitation, diethylaminoethyl cellulose ion exchange and Sephadex gel filtration chromatography. A superoxide dismutase Parallelogram-Shaped Crystal was formed after packing at 4 °C for two days. The optimum pH was 8, and the optimum temperature was 40 °C. The Crystal was relatively stable at temperatures below 60 °C and at pH values from 5 to 10. The subunit size was determined to be approximately 27.5 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. It was also demonstrated that the enzyme is an iron-containing superoxide dismutase based on its sensitivity to inhibitors of potassium cyanide, hydrogen peroxide and chloroform-ethanol and based on the results of ultraviolet absorption spectroscopy.
Bing Chen - One of the best experts on this subject based on the ideXlab platform.
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Purification and Partial Properties of a Superoxide Dismutase from Agave Hybrid No.11648
Journal of the Chinese Chemical Society, 2012Co-Authors: Bing Chen, Liang Chen, Li-jun Gao, Rui Xiao, Pei-lian Liu, Bi-lian Chen, Yun-xia Zhang, Jun YaoAbstract:A superoxide dismutase enzyme was extracted from the leaves of Agave Hybrid No.11648 using homogenisation, ammonium sulphate fractional precipitation, diethylaminoethyl cellulose ion exchange and Sephadex gel filtration chromatography. A superoxide dismutase Parallelogram-Shaped Crystal was formed after packing at 4 °C for two days. The optimum pH was 8, and the optimum temperature was 40 °C. The Crystal was relatively stable at temperatures below 60 °C and at pH values from 5 to 10. The subunit size was determined to be approximately 27.5 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. It was also demonstrated that the enzyme is an iron-containing superoxide dismutase based on its sensitivity to inhibitors of potassium cyanide, hydrogen peroxide and chloroform-ethanol and based on the results of ultraviolet absorption spectroscopy.
Liang Chen - One of the best experts on this subject based on the ideXlab platform.
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Purification and Partial Properties of a Superoxide Dismutase from Agave Hybrid No.11648
Journal of the Chinese Chemical Society, 2012Co-Authors: Bing Chen, Liang Chen, Li-jun Gao, Rui Xiao, Pei-lian Liu, Bi-lian Chen, Yun-xia Zhang, Jun YaoAbstract:A superoxide dismutase enzyme was extracted from the leaves of Agave Hybrid No.11648 using homogenisation, ammonium sulphate fractional precipitation, diethylaminoethyl cellulose ion exchange and Sephadex gel filtration chromatography. A superoxide dismutase Parallelogram-Shaped Crystal was formed after packing at 4 °C for two days. The optimum pH was 8, and the optimum temperature was 40 °C. The Crystal was relatively stable at temperatures below 60 °C and at pH values from 5 to 10. The subunit size was determined to be approximately 27.5 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. It was also demonstrated that the enzyme is an iron-containing superoxide dismutase based on its sensitivity to inhibitors of potassium cyanide, hydrogen peroxide and chloroform-ethanol and based on the results of ultraviolet absorption spectroscopy.
Li-jun Gao - One of the best experts on this subject based on the ideXlab platform.
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Purification and Partial Properties of a Superoxide Dismutase from Agave Hybrid No.11648
Journal of the Chinese Chemical Society, 2012Co-Authors: Bing Chen, Liang Chen, Li-jun Gao, Rui Xiao, Pei-lian Liu, Bi-lian Chen, Yun-xia Zhang, Jun YaoAbstract:A superoxide dismutase enzyme was extracted from the leaves of Agave Hybrid No.11648 using homogenisation, ammonium sulphate fractional precipitation, diethylaminoethyl cellulose ion exchange and Sephadex gel filtration chromatography. A superoxide dismutase Parallelogram-Shaped Crystal was formed after packing at 4 °C for two days. The optimum pH was 8, and the optimum temperature was 40 °C. The Crystal was relatively stable at temperatures below 60 °C and at pH values from 5 to 10. The subunit size was determined to be approximately 27.5 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. It was also demonstrated that the enzyme is an iron-containing superoxide dismutase based on its sensitivity to inhibitors of potassium cyanide, hydrogen peroxide and chloroform-ethanol and based on the results of ultraviolet absorption spectroscopy.
Rui Xiao - One of the best experts on this subject based on the ideXlab platform.
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Purification and Partial Properties of a Superoxide Dismutase from Agave Hybrid No.11648
Journal of the Chinese Chemical Society, 2012Co-Authors: Bing Chen, Liang Chen, Li-jun Gao, Rui Xiao, Pei-lian Liu, Bi-lian Chen, Yun-xia Zhang, Jun YaoAbstract:A superoxide dismutase enzyme was extracted from the leaves of Agave Hybrid No.11648 using homogenisation, ammonium sulphate fractional precipitation, diethylaminoethyl cellulose ion exchange and Sephadex gel filtration chromatography. A superoxide dismutase Parallelogram-Shaped Crystal was formed after packing at 4 °C for two days. The optimum pH was 8, and the optimum temperature was 40 °C. The Crystal was relatively stable at temperatures below 60 °C and at pH values from 5 to 10. The subunit size was determined to be approximately 27.5 kDa by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. It was also demonstrated that the enzyme is an iron-containing superoxide dismutase based on its sensitivity to inhibitors of potassium cyanide, hydrogen peroxide and chloroform-ethanol and based on the results of ultraviolet absorption spectroscopy.
