The Experts below are selected from a list of 24576 Experts worldwide ranked by ideXlab platform
Sathaporn Jittapalapong - One of the best experts on this subject based on the ideXlab platform.
-
Diagnosis of a Trypanosoma lewisi-like (Herpetosoma) infection in a sick infant from Thailand
Journal of Medical Microbiology, 2007Co-Authors: Nachai Sarataphan, Montakan Vongpakorn, Bandit Nuansrichay, Nonglux Autarkool, Tiemjan Keowkarnkah, Pranee Rodtian, Roger W. Stich, Sathaporn JittapalapongAbstract:Trypanosomes were observed in a peripheral blood smear from a 45-day-old Thai infant displaying fever, anaemia, cough and anorexia. Human trypanosomiasis is not endemic to Thailand, so Parasite Identification was undertaken to determine likely sources of the infection. Several morphological parameters of the trypanosomes were similar to those of Trypanosoma evansi and statistically different from those of Trypanosoma lewisi-like Parasites from a naturally infected indigenous rat. However, duplicate PCR assays with primers flanking trypanosome rRNA internal transcribed spacer 1 (ITS1) resulted in amplicons of approximately 623 bp that corresponded to the expected size for T. lewisi-like Parasites. The ITS1 sequence from the infant's blood was 98 and 49 % identical to T. lewisi and T. evansi sequences, respectively. Based on molecular results, it was concluded that the infant was infected with a T. lewisi-like (Herpetosoma) species.
-
Case Report Diagnosis of a Trypanosoma lewisi-like (Herpetosoma) infection in a sick infant from Thailand
2007Co-Authors: Nachai Sarataphan, Montakan Vongpakorn, Bandit Nuansrichay, Nonglux Autarkool, Tiemjan Keowkarnkah, Pranee Rodtian, Roger W. Stich, Sathaporn JittapalapongAbstract:Trypanosomes were observed in a peripheral blood smear from a 45-day-old Thai infant displaying fever, anaemia, cough and anorexia. Human trypanosomiasis is not endemic to Thailand, so Parasite Identification was undertaken to determine likely sources of the infection. Several morphological parameters of the trypanosomes were similar to those of Trypanosoma evansi and statistically different from those of Trypanosoma lewisi-like Parasites from a naturally infected indigenous rat. However, duplicate PCR assays with primers flanking trypanosome rRNA internal transcribed spacer 1 (ITS1) resulted in amplicons of ~623 bp that corresponded to the expected size for T. lewisi-like Parasites. The ITS1 sequence from the infant’s blood was 98 and 49% identical to T. lewisi and T. evansi sequences, respectively. Based on molecular results, it was concluded that the infant was infected with a T. lewisi-like (Herpetosoma) species.
Peter Deplazes - One of the best experts on this subject based on the ideXlab platform.
-
Echinococcosis: diagnosis and diagnostic interpretation in population studies
Trends in parasitology, 2009Co-Authors: Paul R. Torgerson, Peter DeplazesAbstract:Diagnosis is a basic component of population studies on echinococcosis. Other than careful necropsy in animals, there is no perfect gold standard. In the definitive host, techniques for direct Parasite Identification include copro-antigen and copro-DNA detection. In intermediate hosts, necropsy is typically used. In humans, diagnostic imaging and serology are both widely employed. The use of multiple parallel testing or an additional confirmatory test (or tests) in a diagnostic strategy can overcome the lack of a perfect gold standard. This will yield valuable information at population and individual levels, providing the study is well designed and any shortcomings of the tests are incorporated into the analysis. Here, we discuss analytical approaches to population studies of echinococcosis.
-
Influence of urbanization on the epidemiology of intestinal helminths of the red fox (Vulpes vulpes) in Geneva, Switzerland
Parasitology Research, 2007Co-Authors: Leslie A. Reperant, Daniel Hegglin, Claude Fischer, Lucia Kohler, Jean-marc Weber, Peter DeplazesAbstract:Dixenous helminths that depend on rodent intermediate hosts are supposed to be negatively affected by urbanization due to lower supply of rodents in urbanized environments. Prevalence rates of dixenous, non-strictly monoxenous, and monoxenous helminths in 228 red foxes ( Vulpes vulpes ) along a gradient of increasing urbanization were assessed by morphological Parasite Identification in the city of Geneva, Switzerland. Multivariate analyses for the five most prevalent helminth species or genera revealed a significant decrease of prevalence rates for the dixenous helminths Echinococcus multilocularis and Taenia spp. from the rural (52.1 and 54.3%, respectively) to the urban area (30.0 and 20.0%, respectively), but not for the monoxenous nematode Uncinaria stenocephala (overall prevalence of 78.2%) and the non-strictly monoxenous nematode Toxocara canis (overall prevalence of 44.3%). The lower prevalence of Toxascaris leonina in the urban area (8.0%) compared to the rural area (59.6%) raises the question of whether rodent paratenic hosts play a major role for the population dynamics of this species.
Jacques Dereure - One of the best experts on this subject based on the ideXlab platform.
-
Visceral leishmaniasis in eastern Sudan: Parasite Identification in humans and dogs; host-Parasite relationships.
Microbes and infection, 2003Co-Authors: Jacques Dereure, Sayda El-safi, Bruno Bucheton, Mickaël Boni, Musa Mohamed Kheir, Bernard Davoust, Francine Pratlong, Eric Feugier, Monique Lambert, Alain DesseinAbstract:In 1996, an epidemic outbreak of visceral leishmaniasis (VL) started in Barbar el Fugara, a village in Gedarif State (eastern Sudan). From 1997 to 2000, regular epidemiological studies were carried out in the human population, as well as in mammals and sand flies. In symptomatic patients, 46/69 lymph node, 6/20 post kala-azar dermal leishmaniasis (PKDL) and 1/4 cutaneous cultures in NNN medium were positive. In 69 dogs, 23/79 lymph node cultures were positive. In other mammals (47 rodents, five donkeys, one mongoose and one monkey) spleen and/or blood cultures were negative. Characterization of isolated strains (by starch gel electrophoresis and isoelectrofocusing) identified three zymodemes of Leishmania donovani, two of L. infantum and two of L. archibaldi complexes from patient samples and three zymodemes of L. donovani, three of L. infantum and two of L. archibaldi complexes from dog samples. Five of them were present in both man and dog. For the first time, a strain from a PKDL case was identified as L. infantum, and a child had the same L. infantum zymodeme in VL and in subsequent PKDL. Blood samples from dogs were studied by immunofluorescent antibody test (IFAT). The seroprevalence in dogs was 72.5%, 74.3% and 42.9% in 1998, 1999 and 2000, respectively. By using CDC miniature light traps 12 745 sand flies were collected and then identified. Phlebotomus papatasi (7%) and P. orientalis (5%) were sympatric, mainly inside homes (85% and 75%, respectively). These results, the relative stability of seroprevalence in dogs and the intradomiciliar presence of P. orientalis, known as a vector of VL in Sudan, suggest several hypotheses: (i) man is responsible for the disease in dogs, (ii) the dog is the reservoir of VL, (iii) the dog is an intermediate host between a possible sylvatic cycle and the anthroponotic cycle. More extensive studies are needed to assess the transmission cycle of VL in this area of Sudan.
-
Haemoculture as a tool for diagnosing visceral leishmaniasis in HIV-negative and HIV-positive patients: interest for Parasite Identification.
Bulletin of the World Health Organization, 1998Co-Authors: Jacques Dereure, F. Pratlong, J. Reynes, D. Basset, P. Bastien, J.p. DedetAbstract:Between May 1993 and June 1996, 65 adults infected with human immunodeficiency virus (HIV) and 30 HIV-negative patients (8 children and 22 adults) from the Mediterranean region with symptoms that included at least fever were examined for Leishmania. A total of 128 bone marrow and 128 peripheral venous blood samples were taken and cultured on NNN medium. At the initial diagnosis, 14 (6 HIV-positive and 8 HIV-negative) of 15 patients with a positive blood culture also had a positive bone marrow culture. Two patients (1 HIV-positive and the other HIV-negative) had a positive bone marrow culture but a negative blood culture. During post-therapeutic check-ups, 7 out of 8 patients with a positive blood culture (6 HIV-positive and 1 HIV-negative) also had a positive bone marrow culture. On the other hand, three patients (2 HIV-positive and 1 HIV-negative) had a positive bone marrow but a negative blood culture. Relapses were more frequent (9/65 vs. 3/30) and the demonstration of Leishmania in the blood was commoner (6/65 vs. 2/30) in the HIV-positive than the HIV-negative patients. Stocks were identified by their isoenzymes: MON-1 from four HIV-positive and eight HIV-negative patients, MON-28 from one HIV-positive patient and MON-29 from another. For each patient, the same zymodeme was found in bone marrow and blood cultures, both at initial diagnosis and at follow-up.
Nachai Sarataphan - One of the best experts on this subject based on the ideXlab platform.
-
Diagnosis of a Trypanosoma lewisi-like (Herpetosoma) infection in a sick infant from Thailand
Journal of Medical Microbiology, 2007Co-Authors: Nachai Sarataphan, Montakan Vongpakorn, Bandit Nuansrichay, Nonglux Autarkool, Tiemjan Keowkarnkah, Pranee Rodtian, Roger W. Stich, Sathaporn JittapalapongAbstract:Trypanosomes were observed in a peripheral blood smear from a 45-day-old Thai infant displaying fever, anaemia, cough and anorexia. Human trypanosomiasis is not endemic to Thailand, so Parasite Identification was undertaken to determine likely sources of the infection. Several morphological parameters of the trypanosomes were similar to those of Trypanosoma evansi and statistically different from those of Trypanosoma lewisi-like Parasites from a naturally infected indigenous rat. However, duplicate PCR assays with primers flanking trypanosome rRNA internal transcribed spacer 1 (ITS1) resulted in amplicons of approximately 623 bp that corresponded to the expected size for T. lewisi-like Parasites. The ITS1 sequence from the infant's blood was 98 and 49 % identical to T. lewisi and T. evansi sequences, respectively. Based on molecular results, it was concluded that the infant was infected with a T. lewisi-like (Herpetosoma) species.
-
Case Report Diagnosis of a Trypanosoma lewisi-like (Herpetosoma) infection in a sick infant from Thailand
2007Co-Authors: Nachai Sarataphan, Montakan Vongpakorn, Bandit Nuansrichay, Nonglux Autarkool, Tiemjan Keowkarnkah, Pranee Rodtian, Roger W. Stich, Sathaporn JittapalapongAbstract:Trypanosomes were observed in a peripheral blood smear from a 45-day-old Thai infant displaying fever, anaemia, cough and anorexia. Human trypanosomiasis is not endemic to Thailand, so Parasite Identification was undertaken to determine likely sources of the infection. Several morphological parameters of the trypanosomes were similar to those of Trypanosoma evansi and statistically different from those of Trypanosoma lewisi-like Parasites from a naturally infected indigenous rat. However, duplicate PCR assays with primers flanking trypanosome rRNA internal transcribed spacer 1 (ITS1) resulted in amplicons of ~623 bp that corresponded to the expected size for T. lewisi-like Parasites. The ITS1 sequence from the infant’s blood was 98 and 49% identical to T. lewisi and T. evansi sequences, respectively. Based on molecular results, it was concluded that the infant was infected with a T. lewisi-like (Herpetosoma) species.
Alain Dessein - One of the best experts on this subject based on the ideXlab platform.
-
Visceral leishmaniasis in eastern Sudan: Parasite Identification in humans and dogs; host-Parasite relationships.
Microbes and infection, 2003Co-Authors: Jacques Dereure, Sayda El-safi, Bruno Bucheton, Mickaël Boni, Musa Mohamed Kheir, Bernard Davoust, Francine Pratlong, Eric Feugier, Monique Lambert, Alain DesseinAbstract:In 1996, an epidemic outbreak of visceral leishmaniasis (VL) started in Barbar el Fugara, a village in Gedarif State (eastern Sudan). From 1997 to 2000, regular epidemiological studies were carried out in the human population, as well as in mammals and sand flies. In symptomatic patients, 46/69 lymph node, 6/20 post kala-azar dermal leishmaniasis (PKDL) and 1/4 cutaneous cultures in NNN medium were positive. In 69 dogs, 23/79 lymph node cultures were positive. In other mammals (47 rodents, five donkeys, one mongoose and one monkey) spleen and/or blood cultures were negative. Characterization of isolated strains (by starch gel electrophoresis and isoelectrofocusing) identified three zymodemes of Leishmania donovani, two of L. infantum and two of L. archibaldi complexes from patient samples and three zymodemes of L. donovani, three of L. infantum and two of L. archibaldi complexes from dog samples. Five of them were present in both man and dog. For the first time, a strain from a PKDL case was identified as L. infantum, and a child had the same L. infantum zymodeme in VL and in subsequent PKDL. Blood samples from dogs were studied by immunofluorescent antibody test (IFAT). The seroprevalence in dogs was 72.5%, 74.3% and 42.9% in 1998, 1999 and 2000, respectively. By using CDC miniature light traps 12 745 sand flies were collected and then identified. Phlebotomus papatasi (7%) and P. orientalis (5%) were sympatric, mainly inside homes (85% and 75%, respectively). These results, the relative stability of seroprevalence in dogs and the intradomiciliar presence of P. orientalis, known as a vector of VL in Sudan, suggest several hypotheses: (i) man is responsible for the disease in dogs, (ii) the dog is the reservoir of VL, (iii) the dog is an intermediate host between a possible sylvatic cycle and the anthroponotic cycle. More extensive studies are needed to assess the transmission cycle of VL in this area of Sudan.
