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Paschalisadam Molyvdas - One of the best experts on this subject based on the ideXlab platform.
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salbutamol modulation of ion transport in sheep Parietal Pleura is protein dependent
Biological & Pharmaceutical Bulletin, 2011Co-Authors: Sotirios G Zarogiannis, Paschalisadam Molyvdas, Chrisi Hatzoglou, Aristotelis S Filippidis, Konstantinos I GourgoulianisAbstract:The formation of Pleural effusion during pulmonary edema is an important physiological mechanism of resolution of alveolar flooding. In cases of pulmonary edema resulting from acute respiratory distress syndrome (ARDS) these effusions are exudative, having high protein load. To this end, the effect of salbutamol in the presence of protein, on the ion transport properties of the sheep Parietal Pleura was investigated by Ussing chamber experiments. Our results show that salbutamol increases ion transport in the presence of protein in sheep Parietal Pleura by stimulation of β(2)-adrenergic receptors since this effect was completely abolished by the specific β(2)-adrenergic blocker, ICI-118551. This finding may be of importance regarding the acceleration of the resolution of protein-rich Pleural effusions occurring in cases of ARDS.
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low glucose level and low ph alter the electrochemical function of human Parietal Pleura
European Respiratory Journal, 2007Co-Authors: Vasileios K Kouritas, Chrisi Hatzoglou, Konstantinos I Gourgoulianis, Christophoros N Foroulis, A Hevas, Paschalisadam MolyvdasAbstract:The aim of the present study was to investigate whether low glucose and pH level, which are usually measured in complicated Pleural effusions, alter the electrochemical function of healthy human Parietal Pleura. Parietal Pleural pieces were stripped from 66 patients during thoracic surgery and were mounted in Ussing chambers. Krebs’ solutions containing different glucose levels (0, 40 and 100 mg) and balanced at different pH levels (7.4, 7.3 and 7.2) were added to the Pleural cavity surface of the pieces. Transmesothelial potential difference was measured at various time-points as an electrophysiological variable and transmesothelial resistance ( R TM ) was calculated using Ohm9s law. When normal-glucose Krebs at pH 7.45 was used, R TM remained unchanged over time, but when low-glucose Krebs was used, R TM decreased. Krebs without glucose caused the greatest decrease in R TM . Use of low-pH Krebs decreased R TM . The lower the pH of the Krebs, the faster the decrease in R TM and the greater the effect. The decrease in R TM was greater with low-pH than with low-glucose Krebs. Low glucose and low pH caused an additive decrease in R TM . Low glucose concentration and low pH cause alteration of the electrochemical function of human Parietal Pleura and could act as agents that lead to further exudate progression.
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comparison of the electrophysiological properties of the sheep isolated costal and diaphragmatic Parietal Pleura
Clinical and Experimental Pharmacology and Physiology, 2007Co-Authors: Sotirios G Zarogiannis, Chrisi Hatzoglou, Konstantinos I Gourgoulianis, Ioannis Stefanidis, Maria Ioannou, Efrosini Paraskeva, Paschalisadam MolyvdasAbstract:1. Pleural permeability may contribute to Pleural fluid turnover. The transmesothelial resistance (R(TM)), is an established surrogate of mesothelial permeability. The aim of the present study was to compare the electrophysiological properties of costal and diaphragmatic Parietal Pleura. 2. Specimens of the Parietal Pleura were isolated from 12 adult sheep from the chest wall and the diaphragm. Electrophysiological measurements were conducted with the Ussing system. Specimens of the Parietal Pleura of both types (diaphragmatic and costal) were compared histologically and total protein content measurements were also made. 3. The R(TM) of the diaphragmatic Parietal Pleura was significantly higher than that of the costal Parietal Pleura throughout the experiment. The diaphragmatic Parietal Pleura contains more cuboidal cells than the costal Parietal Pleura and its protein content was higher, however this difference was not statistically significant. 4. The costal Parietal Pleura consists of a more 'leaky' mesothelium than the diaphragmatic Pleura. The morphological differences between the two types of Parietal Pleura may underline the electrophysiological findings.
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mu opioid influence on transmesothelial resistance of isolated sheep Pleura and Parietal pericardium
European Journal of Pharmacology, 2006Co-Authors: Konstantinos Vogiatzidis, Chrisi Hatzoglou, Konstantinos I Gourgoulianis, Sotirios G Zarogiannis, Galatia Matafia, Paschalisadam MolyvdasAbstract:The effect of morphine (mu-opioid receptor agonist) on the transmesothelial resistance (R(TM)) of sheep's Pleura and Parietal pericardium was studied using the Ussing chamber technique. Basal transmesothelial resistance of Parietal Pleura was found to be 19.57+/-0.32 Omega cm2 and of visceral Pleura was found to be 19.41+/-0.31 Omega cm2, whereas that of Parietal pericardium was found to be 22.83+/-0.4 Omega cm2. Immediately after the addition of morphine (10(-9) M) both apically and basolaterally on the Parietal Pleura and Parietal pericardium, these values were significantly increased (P<0.05). On the contrary, addition of morphine (10(-9) M) resulted in a rapid increase, only when placed basolaterally on the visceral Pleura (P<0.05). In conclusion, our findings suggest that morphine, probably through mu-opioid stimulation, increases in vitro the transmesothelial resistance of the Parietal Pleura, of the visceral Pleura when added basolaterally and of the Parietal pericardium.
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Effect of amiloride in human and sheep Parietal Pleura.
Respiratory physiology & neurobiology, 2002Co-Authors: S Sarkos, Konstantinos I Gourgoulianis, Chrisi Hatzoglou, J Dahabre, Paschalisadam MolyvdasAbstract:The fluid and solute transport properties of human Parietal Pleura were studied and compared with sheep Parietal Pleura in vitro. The Pleura was mounted as a planar sheet between Ussing-type chambers. The results presented are the mean values of nine different experiments. The transepithelial electrical resistance (R(TE)) of both Pleurae species was measured before and after the addition of amiloride in both sides of Pleura. The R(TE) for human was 25.74 +/- 1.23 Ohm x cm(2), while for the sheep it was 38.18 +/- 0.83 Ohm x cm(2). The addition of amiloride to the serosal bathing solution increased the R(TE) of human Pleura to 30.48 +/- 1.01 Ohm x cm(2) and sheep Pleura to 40.32 +/- 0.82 Ohm x cm(2), while amiloride had no effect on the basolateral side. From the above, it is strongly suggested that the human Pleura seems to be more leaky than sheep Pleura. Although the R(TE) was increased in both Pleurae, the elevation in human Pleura was significantly higher, thus results from experiments in sheep Pleura could only partly be extrapolated in human Pleura.
Chrisi Hatzoglou - One of the best experts on this subject based on the ideXlab platform.
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salbutamol modulation of ion transport in sheep Parietal Pleura is protein dependent
Biological & Pharmaceutical Bulletin, 2011Co-Authors: Sotirios G Zarogiannis, Paschalisadam Molyvdas, Chrisi Hatzoglou, Aristotelis S Filippidis, Konstantinos I GourgoulianisAbstract:The formation of Pleural effusion during pulmonary edema is an important physiological mechanism of resolution of alveolar flooding. In cases of pulmonary edema resulting from acute respiratory distress syndrome (ARDS) these effusions are exudative, having high protein load. To this end, the effect of salbutamol in the presence of protein, on the ion transport properties of the sheep Parietal Pleura was investigated by Ussing chamber experiments. Our results show that salbutamol increases ion transport in the presence of protein in sheep Parietal Pleura by stimulation of β(2)-adrenergic receptors since this effect was completely abolished by the specific β(2)-adrenergic blocker, ICI-118551. This finding may be of importance regarding the acceleration of the resolution of protein-rich Pleural effusions occurring in cases of ARDS.
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Effect of histamine on the electrophysiology of the human Parietal Pleura.
Molecular and cellular endocrinology, 2010Co-Authors: Vasileios K Kouritas, Konstantinos I Gourgoulianis, K. Tepetes, A. Tsantsaridou, Nikolaos Tsilimingas, Chrisi HatzoglouAbstract:Abstract Introduction Histamine is involved in the pathogenesis of numerous diseases and regulates the permeability of different tissues. The aim of this study is to investigate the effects of histamine on the electrophysiology of human Parietal Pleura and the underlying mechanisms involved. Materials and methods Pleural specimens were obtained from patients subjected to thoracic surgery and were mounted in Ussing chambers. Histamine solutions (1 μM to 1 mM) were applied in native and pretreated specimens with dimetindene maleate, cetirizine, ranitidine, amiloride and ouabain. Trans-mesothelial resistance was determined (RTM). Results Histamine induced a rapid RTM increase on the mesothelial (p = 0.008) and a decrease on the interstitial surface (p = 0.029). This effect was dose-dependent and was totally abolished by dimetindene maleate, cetirizine and amiloride and partially by ranitidine and ouabain. Conclusions Histamine induces acute electrochemical changes in human Pleura mainly via interaction with the H1 and partially with the H2 histamine receptors. It also interferes with trans-cellular permeability and therefore may participate in Pleural fluid recycling.
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human Parietal Pleura present electrophysiology variations according to location in Pleural cavity
Interactive Cardiovascular and Thoracic Surgery, 2008Co-Authors: Vasileios K Kouritas, Chrisi Hatzoglou, Christophoros N Foroulis, Konstantinos I GourgoulianisAbstract:The aim of the study was to investigate if human Pleura from different anatomical locations presents electrophysiology differences. Specimens were stripped over the 2nd-5th rib (cranial), 8th-10th rib (caudal), and mediastinum during open surgery and were mounted between Ussing chambers. Amiloride and ouabain were added towards mesothelial surface and trans-mesothelial potential difference (PD) was measured after 1, 5, 10 and 20 min. Trans-membrane resistance (R) was calculated from Ohm's law. R increased after amiloride addition, for cranial (net increase of 0.40 Omega x cm(2)) and caudal (1.16 Omega x cm(2)) Pleural pieces. Mediastinal Pleura R remained unchanged (0.09 Omega x cm(2)). R increase was higher for caudal than cranial (P=0.029) or mediastinal tissues (P=0.002). R increased after ouabain addition for caudal (1.35 Omega x cm(2)) and cranial (0.56 Omega x cm(2)) Pleural pieces. Mediastinal Pleural tissue did not respond (0.20 Omega x cm(2)). Caudally located Pleura responded greater than cranial (P=0.043) or mediastinal (P=0.003) Pleural tissues. Human Pleura shows electrophysiology differences according to the location within the Pleural cavity. Surgeons may waste mediastinal Pleura when needed but should leave intact caudal Parietal Pleura, which seems to be electrophysiologically the most important part of the Pleural cavity.
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low glucose level and low ph alter the electrochemical function of human Parietal Pleura
European Respiratory Journal, 2007Co-Authors: Vasileios K Kouritas, Chrisi Hatzoglou, Konstantinos I Gourgoulianis, Christophoros N Foroulis, A Hevas, Paschalisadam MolyvdasAbstract:The aim of the present study was to investigate whether low glucose and pH level, which are usually measured in complicated Pleural effusions, alter the electrochemical function of healthy human Parietal Pleura. Parietal Pleural pieces were stripped from 66 patients during thoracic surgery and were mounted in Ussing chambers. Krebs’ solutions containing different glucose levels (0, 40 and 100 mg) and balanced at different pH levels (7.4, 7.3 and 7.2) were added to the Pleural cavity surface of the pieces. Transmesothelial potential difference was measured at various time-points as an electrophysiological variable and transmesothelial resistance ( R TM ) was calculated using Ohm9s law. When normal-glucose Krebs at pH 7.45 was used, R TM remained unchanged over time, but when low-glucose Krebs was used, R TM decreased. Krebs without glucose caused the greatest decrease in R TM . Use of low-pH Krebs decreased R TM . The lower the pH of the Krebs, the faster the decrease in R TM and the greater the effect. The decrease in R TM was greater with low-pH than with low-glucose Krebs. Low glucose and low pH caused an additive decrease in R TM . Low glucose concentration and low pH cause alteration of the electrochemical function of human Parietal Pleura and could act as agents that lead to further exudate progression.
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comparison of the electrophysiological properties of the sheep isolated costal and diaphragmatic Parietal Pleura
Clinical and Experimental Pharmacology and Physiology, 2007Co-Authors: Sotirios G Zarogiannis, Chrisi Hatzoglou, Konstantinos I Gourgoulianis, Ioannis Stefanidis, Maria Ioannou, Efrosini Paraskeva, Paschalisadam MolyvdasAbstract:1. Pleural permeability may contribute to Pleural fluid turnover. The transmesothelial resistance (R(TM)), is an established surrogate of mesothelial permeability. The aim of the present study was to compare the electrophysiological properties of costal and diaphragmatic Parietal Pleura. 2. Specimens of the Parietal Pleura were isolated from 12 adult sheep from the chest wall and the diaphragm. Electrophysiological measurements were conducted with the Ussing system. Specimens of the Parietal Pleura of both types (diaphragmatic and costal) were compared histologically and total protein content measurements were also made. 3. The R(TM) of the diaphragmatic Parietal Pleura was significantly higher than that of the costal Parietal Pleura throughout the experiment. The diaphragmatic Parietal Pleura contains more cuboidal cells than the costal Parietal Pleura and its protein content was higher, however this difference was not statistically significant. 4. The costal Parietal Pleura consists of a more 'leaky' mesothelium than the diaphragmatic Pleura. The morphological differences between the two types of Parietal Pleura may underline the electrophysiological findings.
Konstantinos I Gourgoulianis - One of the best experts on this subject based on the ideXlab platform.
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salbutamol modulation of ion transport in sheep Parietal Pleura is protein dependent
Biological & Pharmaceutical Bulletin, 2011Co-Authors: Sotirios G Zarogiannis, Paschalisadam Molyvdas, Chrisi Hatzoglou, Aristotelis S Filippidis, Konstantinos I GourgoulianisAbstract:The formation of Pleural effusion during pulmonary edema is an important physiological mechanism of resolution of alveolar flooding. In cases of pulmonary edema resulting from acute respiratory distress syndrome (ARDS) these effusions are exudative, having high protein load. To this end, the effect of salbutamol in the presence of protein, on the ion transport properties of the sheep Parietal Pleura was investigated by Ussing chamber experiments. Our results show that salbutamol increases ion transport in the presence of protein in sheep Parietal Pleura by stimulation of β(2)-adrenergic receptors since this effect was completely abolished by the specific β(2)-adrenergic blocker, ICI-118551. This finding may be of importance regarding the acceleration of the resolution of protein-rich Pleural effusions occurring in cases of ARDS.
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Effect of histamine on the electrophysiology of the human Parietal Pleura.
Molecular and cellular endocrinology, 2010Co-Authors: Vasileios K Kouritas, Konstantinos I Gourgoulianis, K. Tepetes, A. Tsantsaridou, Nikolaos Tsilimingas, Chrisi HatzoglouAbstract:Abstract Introduction Histamine is involved in the pathogenesis of numerous diseases and regulates the permeability of different tissues. The aim of this study is to investigate the effects of histamine on the electrophysiology of human Parietal Pleura and the underlying mechanisms involved. Materials and methods Pleural specimens were obtained from patients subjected to thoracic surgery and were mounted in Ussing chambers. Histamine solutions (1 μM to 1 mM) were applied in native and pretreated specimens with dimetindene maleate, cetirizine, ranitidine, amiloride and ouabain. Trans-mesothelial resistance was determined (RTM). Results Histamine induced a rapid RTM increase on the mesothelial (p = 0.008) and a decrease on the interstitial surface (p = 0.029). This effect was dose-dependent and was totally abolished by dimetindene maleate, cetirizine and amiloride and partially by ranitidine and ouabain. Conclusions Histamine induces acute electrochemical changes in human Pleura mainly via interaction with the H1 and partially with the H2 histamine receptors. It also interferes with trans-cellular permeability and therefore may participate in Pleural fluid recycling.
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human Parietal Pleura present electrophysiology variations according to location in Pleural cavity
Interactive Cardiovascular and Thoracic Surgery, 2008Co-Authors: Vasileios K Kouritas, Chrisi Hatzoglou, Christophoros N Foroulis, Konstantinos I GourgoulianisAbstract:The aim of the study was to investigate if human Pleura from different anatomical locations presents electrophysiology differences. Specimens were stripped over the 2nd-5th rib (cranial), 8th-10th rib (caudal), and mediastinum during open surgery and were mounted between Ussing chambers. Amiloride and ouabain were added towards mesothelial surface and trans-mesothelial potential difference (PD) was measured after 1, 5, 10 and 20 min. Trans-membrane resistance (R) was calculated from Ohm's law. R increased after amiloride addition, for cranial (net increase of 0.40 Omega x cm(2)) and caudal (1.16 Omega x cm(2)) Pleural pieces. Mediastinal Pleura R remained unchanged (0.09 Omega x cm(2)). R increase was higher for caudal than cranial (P=0.029) or mediastinal tissues (P=0.002). R increased after ouabain addition for caudal (1.35 Omega x cm(2)) and cranial (0.56 Omega x cm(2)) Pleural pieces. Mediastinal Pleural tissue did not respond (0.20 Omega x cm(2)). Caudally located Pleura responded greater than cranial (P=0.043) or mediastinal (P=0.003) Pleural tissues. Human Pleura shows electrophysiology differences according to the location within the Pleural cavity. Surgeons may waste mediastinal Pleura when needed but should leave intact caudal Parietal Pleura, which seems to be electrophysiologically the most important part of the Pleural cavity.
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low glucose level and low ph alter the electrochemical function of human Parietal Pleura
European Respiratory Journal, 2007Co-Authors: Vasileios K Kouritas, Chrisi Hatzoglou, Konstantinos I Gourgoulianis, Christophoros N Foroulis, A Hevas, Paschalisadam MolyvdasAbstract:The aim of the present study was to investigate whether low glucose and pH level, which are usually measured in complicated Pleural effusions, alter the electrochemical function of healthy human Parietal Pleura. Parietal Pleural pieces were stripped from 66 patients during thoracic surgery and were mounted in Ussing chambers. Krebs’ solutions containing different glucose levels (0, 40 and 100 mg) and balanced at different pH levels (7.4, 7.3 and 7.2) were added to the Pleural cavity surface of the pieces. Transmesothelial potential difference was measured at various time-points as an electrophysiological variable and transmesothelial resistance ( R TM ) was calculated using Ohm9s law. When normal-glucose Krebs at pH 7.45 was used, R TM remained unchanged over time, but when low-glucose Krebs was used, R TM decreased. Krebs without glucose caused the greatest decrease in R TM . Use of low-pH Krebs decreased R TM . The lower the pH of the Krebs, the faster the decrease in R TM and the greater the effect. The decrease in R TM was greater with low-pH than with low-glucose Krebs. Low glucose and low pH caused an additive decrease in R TM . Low glucose concentration and low pH cause alteration of the electrochemical function of human Parietal Pleura and could act as agents that lead to further exudate progression.
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comparison of the electrophysiological properties of the sheep isolated costal and diaphragmatic Parietal Pleura
Clinical and Experimental Pharmacology and Physiology, 2007Co-Authors: Sotirios G Zarogiannis, Chrisi Hatzoglou, Konstantinos I Gourgoulianis, Ioannis Stefanidis, Maria Ioannou, Efrosini Paraskeva, Paschalisadam MolyvdasAbstract:1. Pleural permeability may contribute to Pleural fluid turnover. The transmesothelial resistance (R(TM)), is an established surrogate of mesothelial permeability. The aim of the present study was to compare the electrophysiological properties of costal and diaphragmatic Parietal Pleura. 2. Specimens of the Parietal Pleura were isolated from 12 adult sheep from the chest wall and the diaphragm. Electrophysiological measurements were conducted with the Ussing system. Specimens of the Parietal Pleura of both types (diaphragmatic and costal) were compared histologically and total protein content measurements were also made. 3. The R(TM) of the diaphragmatic Parietal Pleura was significantly higher than that of the costal Parietal Pleura throughout the experiment. The diaphragmatic Parietal Pleura contains more cuboidal cells than the costal Parietal Pleura and its protein content was higher, however this difference was not statistically significant. 4. The costal Parietal Pleura consists of a more 'leaky' mesothelium than the diaphragmatic Pleura. The morphological differences between the two types of Parietal Pleura may underline the electrophysiological findings.
P. De Vuyst - One of the best experts on this subject based on the ideXlab platform.
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Chrysotile and tremolite asbestos fibres in the lungs and Parietal Pleura of Corsican goats
Occupational and Environmental Medicine, 2002Co-Authors: Pascal Dumortier, F. Rey, J. R. Viallat, Ingrid Broucke, C. Boutin, P. De VuystAbstract:Background and Aims: Environmental exposures to chrysotile and tremolite from the soil cause Pleural plaques and mesothelioma in northeast Corsica. Goats grazing in the contaminated areas inhale asbestos fibres. We used this natural animal model to study whether these exposures actually result in increased fibre burdens in the lungs and Parietal Pleura. Methods: Ten goats from areas with asbestos outcrops and two from other areas were slaughtered. Fibre content of lung and Parietal Pleural samples was determined by analytical transmission electron microscopy. Results: Both chrysotile and tremolite fibres were detected. In the exposed goats, the geometric mean concentrations of asbestos fibres longer than 1 μm were 0.27 × 10 6 fibres/g dry lung tissue and 1.8 × 10 6 fibres/g dry Pleural tissue. Asbestos fibres were not detected in the lungs of the two control goats. Chrysotile fibres shorter than 5 μm were predominant in the Parietal Pleura. Tremolite fibres accounted for 78% and 86% of the fibres longer than 5 μm in lung and Parietal Pleural samples, respectively. Conclusions: Environmental exposure in northeast Corsica results in detectable chrysotile and tremolite fibre loads in the lung and Parietal Pleura of adult goats. Tremolite fibres of dimensions with a high carcinogenic potency are detected in the Parietal Pleura.
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black spots concentrate oncogenic asbestos fibers in the Parietal Pleura thoracoscopic and mineralogic study
American Journal of Respiratory and Critical Care Medicine, 1996Co-Authors: C. Boutin, F. Rey, J. R. Viallat, P Dumortier, P. De VuystAbstract:Epidemiologic and pathologic data demonstrate that malignant mesothelioma occurs preferentially after exposure to long amphibole asbestos fibers. However, mineralogic studies have rarely detected such fibers in the Parietal Pleura. We hypothesized that the distribution of asbestos fibers in the Pleura was heterogeneous and that they might concentrate in certain areas, as does coal dust in patients showing anthracotic "black spots" of the Parietal Pleura during thoracoscopy. We collected thoracoscopic biopsy samples from these black spots and from normal areas of the Parietal Pleura and lung from 14 subjects (eight with and six without asbestos exposure). Asbestos content was determined by transmission electron microscopy. In exposed subjects, mean fiber concentrations were 12.4 +/- 9.8 x 10(6) fibers/g of dry tissue in lung, 4.1 +/- 1.9 in black spots, and 0.5 +/- 0.2 in normal Pleura. In unexposed patients, concentrations were 0, 0.3 +/- 0.1, and 0, respectively. Amphiboles outnumbered chrysotile in all samples. A total of 22.5% of fibers were > or = 5 microns in length in black spots. A histologic similarity of these black spots with milky spots is suggested by conventional and electron microscopy. We conclude that the distribution of asbestos fibers is heterogeneous in the Parietal Pleura. Indeed, the fibers concentrate in black spots, where they can reach high concentrations. These findings could explain why the Parietal Pleura is the target organ for mesothelioma and plaques.
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carcinogenic asbestos fibers in the Parietal Pleura
1994Co-Authors: Francoise Rey, J. R. Viallat, Christian Boutin, P Dumortier, P. De VuystAbstract:The role of asbestos fibers in the development of lesions of the Parietal Pleura and especially mesothelioma is unclear. Based on the experimental findings of Stanton (1981) with intraPleural asbestos injections in animals, it is generally accepted that fibers more than 8μ in length and less than 0.25 μ in diameter are the most carcinogenic. More recently, the same toxicity was attributed to fibers 5μ or more long (Jaurand 1991). The presence of such fibers is controversial. In man (Boutin 1981, Sebastien 1980, Dodson 1990, Kohyama 1991) as well as in experimental animals (Rey 1993), most of the fibers observed have been short (<3μ). Studies have also demonstrated a lack of correlation between the concentration of asbestos fibers in lung tissue and the results of biopsy of Parietal Pleura samples. This discrepancy has been explained by the heterogeneous topographical distribution of fibers in the Parietal Pleura (Sebastien 1980). Thoracoscopic observation of “anthracotic spots” near lymphatic vessels suggest that exogenous particle may collect there. The purpose of this study was to detect the presence of potentially carnogenic asbestos fibers (i.e. 5μ or longer) in the Parietal Pleura of patients with a history of exposure to asbestos and to determine if fibers collect preferentially in anthracotic areas.
Sotirios G Zarogiannis - One of the best experts on this subject based on the ideXlab platform.
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salbutamol modulation of ion transport in sheep Parietal Pleura is protein dependent
Biological & Pharmaceutical Bulletin, 2011Co-Authors: Sotirios G Zarogiannis, Paschalisadam Molyvdas, Chrisi Hatzoglou, Aristotelis S Filippidis, Konstantinos I GourgoulianisAbstract:The formation of Pleural effusion during pulmonary edema is an important physiological mechanism of resolution of alveolar flooding. In cases of pulmonary edema resulting from acute respiratory distress syndrome (ARDS) these effusions are exudative, having high protein load. To this end, the effect of salbutamol in the presence of protein, on the ion transport properties of the sheep Parietal Pleura was investigated by Ussing chamber experiments. Our results show that salbutamol increases ion transport in the presence of protein in sheep Parietal Pleura by stimulation of β(2)-adrenergic receptors since this effect was completely abolished by the specific β(2)-adrenergic blocker, ICI-118551. This finding may be of importance regarding the acceleration of the resolution of protein-rich Pleural effusions occurring in cases of ARDS.
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comparison of the electrophysiological properties of the sheep isolated costal and diaphragmatic Parietal Pleura
Clinical and Experimental Pharmacology and Physiology, 2007Co-Authors: Sotirios G Zarogiannis, Chrisi Hatzoglou, Konstantinos I Gourgoulianis, Ioannis Stefanidis, Maria Ioannou, Efrosini Paraskeva, Paschalisadam MolyvdasAbstract:1. Pleural permeability may contribute to Pleural fluid turnover. The transmesothelial resistance (R(TM)), is an established surrogate of mesothelial permeability. The aim of the present study was to compare the electrophysiological properties of costal and diaphragmatic Parietal Pleura. 2. Specimens of the Parietal Pleura were isolated from 12 adult sheep from the chest wall and the diaphragm. Electrophysiological measurements were conducted with the Ussing system. Specimens of the Parietal Pleura of both types (diaphragmatic and costal) were compared histologically and total protein content measurements were also made. 3. The R(TM) of the diaphragmatic Parietal Pleura was significantly higher than that of the costal Parietal Pleura throughout the experiment. The diaphragmatic Parietal Pleura contains more cuboidal cells than the costal Parietal Pleura and its protein content was higher, however this difference was not statistically significant. 4. The costal Parietal Pleura consists of a more 'leaky' mesothelium than the diaphragmatic Pleura. The morphological differences between the two types of Parietal Pleura may underline the electrophysiological findings.
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mu opioid influence on transmesothelial resistance of isolated sheep Pleura and Parietal pericardium
European Journal of Pharmacology, 2006Co-Authors: Konstantinos Vogiatzidis, Chrisi Hatzoglou, Konstantinos I Gourgoulianis, Sotirios G Zarogiannis, Galatia Matafia, Paschalisadam MolyvdasAbstract:The effect of morphine (mu-opioid receptor agonist) on the transmesothelial resistance (R(TM)) of sheep's Pleura and Parietal pericardium was studied using the Ussing chamber technique. Basal transmesothelial resistance of Parietal Pleura was found to be 19.57+/-0.32 Omega cm2 and of visceral Pleura was found to be 19.41+/-0.31 Omega cm2, whereas that of Parietal pericardium was found to be 22.83+/-0.4 Omega cm2. Immediately after the addition of morphine (10(-9) M) both apically and basolaterally on the Parietal Pleura and Parietal pericardium, these values were significantly increased (P<0.05). On the contrary, addition of morphine (10(-9) M) resulted in a rapid increase, only when placed basolaterally on the visceral Pleura (P<0.05). In conclusion, our findings suggest that morphine, probably through mu-opioid stimulation, increases in vitro the transmesothelial resistance of the Parietal Pleura, of the visceral Pleura when added basolaterally and of the Parietal pericardium.