The Experts below are selected from a list of 1359 Experts worldwide ranked by ideXlab platform
Purnima S. Kumar - One of the best experts on this subject based on the ideXlab platform.
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Furcation Therapy With Enamel Matrix Derivative: Effects on the Subgingival Microbiome
Journal of periodontology, 2017Co-Authors: Lucas Araujo Queiroz, Renato Corrêa Viana Casarin, Shareef M. Dabdoub, Dimitris N. Tatakis, Enilson Antonio Sallum, Purnima S. KumarAbstract:Background: Although enamel matrix derivative (EMD) has been used to promote periodontal regeneration, little is known of its effect on the microbiome. Therefore, this investigation aims to identify changes in periodontal microbiome after treatment with EMD using a deep-sequencing approach.Methods: Thirty-nine patients with mandibular Class II buccal furcation defects were randomized to beta-tricalcium-phosphate/hydroxyapatite graft (BONE group), EMD+BONE, or EMD alone. Plaque was collected from furcation defects at baseline and 3 and 6 months post-treatment. Bacterial DNA was analyzed using terminal restriction fragment length polymorphism and 16S pyrotag sequencing, resulting in 169,000 classifiable sequences being compared with the Human Oral Microbiome Database. Statistical comparisons were made using parametric tests.Results: At baseline, a total of 422 species were identified from the 39 defects, belonging to Fusobacterium, Pseudomonas, Streptococcus, Filifactor, and Parvimonas. All three regenerati...
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Bacteriology of Periodontal Diseases
Molecular Medical Microbiology, 2015Co-Authors: Eija Könönen, Purnima S. KumarAbstract:Periodontal diseases include a heterogeneous group of chronic inflammatory conditions, which are mainly due to specific oral bacteria enriched in subgingival biofilms. While gingivitis is a reversible inflammatory reaction of gingiva, periodontitis with progressing inflammation at deeper periodontal tissues leads to irreversible connective and bone tissue breakdown. Traditionally, Gram-negative species, such as Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Aggregatibacter actinomycetemcomitans, are considered major culprits of periodontal destruction. P. gingivalis, in particular, can severely affect the ecosystem, even as a minor constituent of the subgingival microbiota, and is seen as a keystone pathogen in periodontitis. Also Gram-positive taxa, such as Filifactor alocis, Parvimonas micra and Eubacterium nodatum, and many not-yet-cultivated phylotypes play a role in periodontal pathogenesis. The introduction of molecular techniques into periodontal microbiology has, indeed, expanded our knowledge about periodontal micro-organisms in health and disease.
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Subgingival Microbial Profiles of Smokers with Periodontitis
Journal of Dental Research, 2010Co-Authors: A.y. Shchipkova, Haikady N. Nagaraja, Purnima S. KumarAbstract:The subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. We tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. Subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16S sequencing. Smokers demonstrated greater abundance of Parvimonas, Fusobacterium, Campylobacter, Bacteroides, and Treponema and lower levels of Veillonella, Neisseria, and Streptococcus. Several uncultivated Peptostreptococci, Parvimonas micra, Campy-lobacter gracilis, Treponema socranskii, Dialister pneumosintes, and Tannerella forsythia were elevated in this group, while Veillonella sp. oral clone B2, Neisseria sp. oral clone 2.24, Streptococcus sanguinis, and Capnocytophaga sp. clone AH015 were at lower levels. The microbial profile...
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subgingival microbial profiles of smokers with periodontitis
Journal of Dental Research, 2010Co-Authors: A.y. Shchipkova, Haikady N. Nagaraja, Purnima S. KumarAbstract:The subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. We tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. Subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16S sequencing. Smokers demonstrated greater abundance of Parvimonas, Fusobacterium, Campylobacter, Bacteroides, and Treponema and lower levels of Veillonella, Neisseria, and Streptococcus. Several uncultivated Peptostreptococci, Parvimonas micra, Campylobacter gracilis, Treponema socranskii, Dialister pneumosintes, and Tannerella forsythia were elevated in this group, while Veillonella sp. oral clone B2, Neisseria sp. oral clone 2.24, Streptococcus sanguinis, and Capnocytophaga sp. clone AH015 were at lower levels. The microbial profile of smoking-associated periodontitis is distinct from that of non-smokers, with significant differences in the prevalence and abundance of disease-associated and health-compatible organisms.
Ali Ahmad - One of the best experts on this subject based on the ideXlab platform.
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New Bacterial Compositions in Root-filled Teeth with Periradicular Lesions
Journal of Endodontics, 2008Co-Authors: Jörg F. Schirrmeister, Anna-lisa Liebenow, Annerose Serr, Annette Wittmer, Klaus Pelz, Elmar Hellwig, Ali AhmadAbstract:The aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. Specimens were sampled from patients undergoing root canal retreatment. The bacteria were characterized by morphologic and biochemical analysis and by 16S rRNA gene sequencing. Microorganisms were detected in 10 of 18 teeth. The majority of positive samples revealed a mixed culture of 2–8 species. In 2 teeth Enterococcus faecalis was the only detected species. For the first time Vagococcus fluvialis was detected in root canals. Solobacterium moorei and Fusobacterium nucleatum were the most prevalent species. Presence of F. nucleatum was associated with the presence of S. moorei in 5 of 7 cases. In all teeth with Parvimonas micra and Dialister invisus, F. nucleatum and S. moorei were found. Moreover, members of additional different genera were detected delivering bacterial compositions that have been not described yet. (J Endod 2009;35:169–174)
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New Bacterial Compositions in Root-filled Teeth with Periradicular Lesions
Journal of endodontics, 2008Co-Authors: Jörg F. Schirrmeister, Anna-lisa Liebenow, Annerose Serr, Annette Wittmer, Klaus Pelz, Elmar Hellwig, Ali AhmadAbstract:The aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. Specimens were sampled from patients undergoing root canal retreatment. The bacteria were characterized by morphologic and biochemical analysis and by 16S rRNA gene sequencing. Microorganisms were detected in 10 of 18 teeth. The majority of positive samples revealed a mixed culture of 2-8 species. In 2 teeth Enterococcus faecalis was the only detected species. For the first time Vagococcus fluvialis was detected in root canals. Solobacterium moorei and Fusobacterium nucleatum were the most prevalent species. Presence of F. nucleatum was associated with the presence of S. moorei in 5 of 7 cases. In all teeth with Parvimonas micra and Dialister invisus, F. nucleatum and S. moorei were found. Moreover, members of additional different genera were detected delivering bacterial compositions that have been not described yet.
Frederic Cadoret - One of the best experts on this subject based on the ideXlab platform.
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Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description
MicrobiologyOpen, 2018Co-Authors: Melhem Bilen, Maxime Mbogning Fonkou, Thi Nguyen, Magali Richez, Ziad Daoud, Pierre Fournier, Didier Raoult, Frederic CadoretAbstract:With the aim of describing the human microbiota by the means of culture methods, culturomics was developed in order to target previously un‐isolated bacterial species and describe it via the taxono‐genomics approach. While performing a descriptive study of the human gut microbiota of the pygmy people, strain Marseille‐P4678T has been isolated from a stool sample of a healthy 39‐year‐old pygmy male. Cells of this strain were Gram‐positive cocci, spore‐forming, non‐motile, catalase‐positive and oxidase‐negative, and grow optimally at 37°C under anaerobic conditions. Its 16S rRNA gene sequence exhibited 89.69% of sequence similarity with Parvimonas micra strain 3119BT (NR 036934.1), its phylogenetically closest species with standing in nomenclature. The genome of strain Marseille‐P4678T is 2,083,161 long with 28.26 mol% of G+C content. Based on its phenotypic, biochemical, genotypic and proteomic profile, this bacterium was classified as a new bacterial genus and species Miniphocibacter massiliensis gen. nov., sp. nov. with the type strain Marseille‐P4678T.
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Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono-genomics description.
MicrobiologyOpen, 2018Co-Authors: Melhem Bilen, Magali Richez, Ziad Daoud, Didier Raoult, Maxime D. Mbogning Fonkou, Thi Tien Nguyen, Pierre E. Fournier, Frederic CadoretAbstract:With the aim of describing the human microbiota by the means of culture methods, culturomics was developed in order to target previously un-isolated bacterial species and describe it via the taxono-genomics approach. While performing a descriptive study of the human gut microbiota of the pygmy people, strain Marseille-P4678T has been isolated from a stool sample of a healthy 39-year-old pygmy male. Cells of this strain were Gram-positive cocci, spore-forming, non-motile, catalase-positive and oxidase-negative, and grow optimally at 37°C under anaerobic conditions. Its 16S rRNA gene sequence exhibited 89.69% of sequence similarity with Parvimonas micra strain 3119BT (NR 036934.1), its phylogenetically closest species with standing in nomenclature. The genome of strain Marseille-P4678T is 2,083,161 long with 28.26 mol% of G+C content. Based on its phenotypic, biochemical, genotypic and proteomic profile, this bacterium was classified as a new bacterial genus and species Miniphocibacter massiliensis gen. nov., sp. nov. with the type strain Marseille-P4678T .
Jörg F. Schirrmeister - One of the best experts on this subject based on the ideXlab platform.
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New Bacterial Compositions in Root-filled Teeth with Periradicular Lesions
Journal of Endodontics, 2008Co-Authors: Jörg F. Schirrmeister, Anna-lisa Liebenow, Annerose Serr, Annette Wittmer, Klaus Pelz, Elmar Hellwig, Ali AhmadAbstract:The aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. Specimens were sampled from patients undergoing root canal retreatment. The bacteria were characterized by morphologic and biochemical analysis and by 16S rRNA gene sequencing. Microorganisms were detected in 10 of 18 teeth. The majority of positive samples revealed a mixed culture of 2–8 species. In 2 teeth Enterococcus faecalis was the only detected species. For the first time Vagococcus fluvialis was detected in root canals. Solobacterium moorei and Fusobacterium nucleatum were the most prevalent species. Presence of F. nucleatum was associated with the presence of S. moorei in 5 of 7 cases. In all teeth with Parvimonas micra and Dialister invisus, F. nucleatum and S. moorei were found. Moreover, members of additional different genera were detected delivering bacterial compositions that have been not described yet. (J Endod 2009;35:169–174)
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New Bacterial Compositions in Root-filled Teeth with Periradicular Lesions
Journal of endodontics, 2008Co-Authors: Jörg F. Schirrmeister, Anna-lisa Liebenow, Annerose Serr, Annette Wittmer, Klaus Pelz, Elmar Hellwig, Ali AhmadAbstract:The aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. Specimens were sampled from patients undergoing root canal retreatment. The bacteria were characterized by morphologic and biochemical analysis and by 16S rRNA gene sequencing. Microorganisms were detected in 10 of 18 teeth. The majority of positive samples revealed a mixed culture of 2-8 species. In 2 teeth Enterococcus faecalis was the only detected species. For the first time Vagococcus fluvialis was detected in root canals. Solobacterium moorei and Fusobacterium nucleatum were the most prevalent species. Presence of F. nucleatum was associated with the presence of S. moorei in 5 of 7 cases. In all teeth with Parvimonas micra and Dialister invisus, F. nucleatum and S. moorei were found. Moreover, members of additional different genera were detected delivering bacterial compositions that have been not described yet.
Melhem Bilen - One of the best experts on this subject based on the ideXlab platform.
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Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description
MicrobiologyOpen, 2018Co-Authors: Melhem Bilen, Maxime Mbogning Fonkou, Thi Nguyen, Magali Richez, Ziad Daoud, Pierre Fournier, Didier Raoult, Frederic CadoretAbstract:With the aim of describing the human microbiota by the means of culture methods, culturomics was developed in order to target previously un‐isolated bacterial species and describe it via the taxono‐genomics approach. While performing a descriptive study of the human gut microbiota of the pygmy people, strain Marseille‐P4678T has been isolated from a stool sample of a healthy 39‐year‐old pygmy male. Cells of this strain were Gram‐positive cocci, spore‐forming, non‐motile, catalase‐positive and oxidase‐negative, and grow optimally at 37°C under anaerobic conditions. Its 16S rRNA gene sequence exhibited 89.69% of sequence similarity with Parvimonas micra strain 3119BT (NR 036934.1), its phylogenetically closest species with standing in nomenclature. The genome of strain Marseille‐P4678T is 2,083,161 long with 28.26 mol% of G+C content. Based on its phenotypic, biochemical, genotypic and proteomic profile, this bacterium was classified as a new bacterial genus and species Miniphocibacter massiliensis gen. nov., sp. nov. with the type strain Marseille‐P4678T.
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Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono-genomics description.
MicrobiologyOpen, 2018Co-Authors: Melhem Bilen, Magali Richez, Ziad Daoud, Didier Raoult, Maxime D. Mbogning Fonkou, Thi Tien Nguyen, Pierre E. Fournier, Frederic CadoretAbstract:With the aim of describing the human microbiota by the means of culture methods, culturomics was developed in order to target previously un-isolated bacterial species and describe it via the taxono-genomics approach. While performing a descriptive study of the human gut microbiota of the pygmy people, strain Marseille-P4678T has been isolated from a stool sample of a healthy 39-year-old pygmy male. Cells of this strain were Gram-positive cocci, spore-forming, non-motile, catalase-positive and oxidase-negative, and grow optimally at 37°C under anaerobic conditions. Its 16S rRNA gene sequence exhibited 89.69% of sequence similarity with Parvimonas micra strain 3119BT (NR 036934.1), its phylogenetically closest species with standing in nomenclature. The genome of strain Marseille-P4678T is 2,083,161 long with 28.26 mol% of G+C content. Based on its phenotypic, biochemical, genotypic and proteomic profile, this bacterium was classified as a new bacterial genus and species Miniphocibacter massiliensis gen. nov., sp. nov. with the type strain Marseille-P4678T .