The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Beverly Barton Rogers - One of the best experts on this subject based on the ideXlab platform.
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Retrospective diagnosis of sickle cell-hemoglobin C disease and Parvovirus Infection by molecular DNA analysis of postmortem tissue
Human Pathology, 1995Co-Authors: Lisa Goldstein, Rochelle Strenger, Stacy Comfort Le, Thomas King, Beverly Barton RogersAbstract:Abstract A previously healthy 30-year-old African-American woman presented with a history of sickle cell trait and a nonspecific prodromal illness with severe bone pain. She experienced rapid clinical deterioration with seizures and cardiorespiratory arrest leading to death. Autopsy showed necrotic bone marrow with extensive bone marrow emboli. Parvovirus Infection was documented by polymerase chain reaction (PCR) although diagnostic intranuclear inclusions or giant pronormoblasts were not present. The diagnosis of sickle cell-hemoglobin C disease (hemoglobin SC disease) was also established postmortem by DNA sequencing of PCR products. This case illustrates the use and versatility of PCR for analysis of formalin-fixed paraffin-embedded autopsy tissue.
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Diagnosis and incidence of fetal Parvovirus Infection in an autopsy series: I. Histology.
Pediatric pathology, 1993Co-Authors: Beverly Barton Rogers, Yvonne Mark, Calvin E. OyerAbstract:This study was undertaken to obtain additional information about the incidence and characteristics of fetal Parvovirus B19 Infection in an unselected autopsy series, and to assess the distribution and quantitation of inclusions in various organs. Autopsy records from 673 fetal and neonatal autopsies performed at Women and Infants′ Hospital during 1985 through 1990 were reviewed. Thirty-two cases of hydrops fetalis were identified, and, of these, 5 had Parvovirus Infection. This gives an incidence of fetal Parvovirus Infection resulting in hydrops fetalis of 0. 7% among all autopsies, and a 16% incidence among cases of hydrops. Thirty-five percent of the cases of hydrops had malformations; a muscular ventricular septal defect was noted in one of the 5 cases of Parvovirus Infection. All 5 Parvovirus cases had characteristic erylhroid nuclear inclusions, and these inclusions were resistant to tissue degenerative changes. The most reliable tissue for histologic diagnosis was the liver, followed by heart and l...
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Diagnosis and incidence of fetal Parvovirus Infection in an autopsy series: II. DNA amplification.
Pediatric pathology, 1993Co-Authors: Yvonne Mark, Beverly Barton Rogers, Calvin E. OyerAbstract:This study evaluates the practical utility of the polymerase chain reaction (PCR) as a diagnostic method for intrauterine fetal Parvovirus Infection in cases of hydrops fetalis. Paraffin-embedded, formalin-fixed fetal tissues from cases of hydrops fetalis were assessed for Parvovirus B19 by histology and PCR in conjunction with 32P hybridization. Of 673 fetal and neonatal autopsies performed at Women and Infants′ Hospital for the years 1985 through 1990, 32 cases were determined to have hydrops fetalis, of which five were positive for Parvovirus Infection by both histology and the PCR. PCR was not used in seven (22%) of the 32 hydrops cases because 1 µg of DNA was not available for study. Histology was as sensitive as PCR in detecting Parvovirus B19 in fetal autopsy tissues from cases of hydrops fetalis, and could be used reliably in each case to diagnose Parvovirus Infection. In our hands, histology is as sensitive as PCR and less labor-intensive. We would reserve PCR for cases without inclusions and wit...
Giancarlo Mari - One of the best experts on this subject based on the ideXlab platform.
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Fetal thrombocytopenia secondary to Parvovirus Infection.
American journal of obstetrics and gynecology, 2007Co-Authors: M. Segata, Rabih Chaoui, Nahla Khalek, Ray O. Bahado-singh, Michael J. Paidas, Giancarlo MariAbstract:Objective The aim of this study was to determine the platelet count in fetuses undergoing cordocentesis for hydrops caused by Parvovirus Infection. Study design Fetal platelets were measured at cordocentesis in 11 pregnant women who underwent the procedure because of fetal ascites and/or hydrops caused by Parvovirus Infection. Thrombocytopenia was defined as mild (platelet count 9 /L), moderate (platelet count ≤ 100 × 10 9 /L), or severe (platelet count to ≤ 50 × 10 9 /L). Paired Student t test was performed to compare the platelet count before and after the transfusion. Results The fetuses underwent 20 cordocenteses. They were thrombocytopenic in 17 and anemic in 15 occasions. The platelet count was reduced after the transfusion ( P 9 /L, respectively). Conclusion Thrombocytopenia is common in fetuses with hydrops caused by Parvovirus Infection, and can cause exsanguination from the umbilical cord puncture site. We recommend platelet transfusion during cordocentesis when there is severe thrombocytopenia.
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noninvasive diagnosis by doppler ultrasonography of fetal anemia resulting from Parvovirus Infection
American Journal of Obstetrics and Gynecology, 2002Co-Authors: Erich Cosmi, Giancarlo Mari, Loredana Delle Chiaie, Laura Detti, Masashi Akiyama, June Murphy, Theodor Stefos, James E Ferguson, David Hunter, Chaurdong HsuAbstract:Abstract Objective: The purpose of this study was to evaluate the feasibility of the middle cerebral artery peak systolic velocity for the detection of fetal anemia in pregnancies that are complicated by Parvovirus B19 Infection. Study Design: Doppler measurements of the middle cerebral artery peak systolic velocity were performed weekly in 32 fetuses at risk for anemia because of maternal Parvovirus Infection documented by the presence of serum immunoglobulin M antibody. The values of the middle cerebral artery peak systolic velocity and hemoglobin were expressed as multiples of the median. These values were plotted on reference ranges that had been established previously. A cordocentesis was performed either because of fetal ascites or when the middle cerebral artery peak systolic velocity values suggested anemia (middle cerebral artery peak systolic velocity, >1.50 multiples of the median). Results: Gestational age at study entry ranged from 15.1 to 37 weeks. There were 17 fetuses with middle cerebral artery peak systolic velocity of >1.50 MoM (group 1). Sixteen cordocenteses were performed in these fetuses. All 16 fetuses were anemic (15 severely and 1 mildly). Thirteen fetuses had signs of hydrops (12 with severe and 1 with mild anemia). Group 2 included 15 fetuses with the middle cerebral artery peak systolic velocity values 1.50 MoM) for the prediction of anemia because of Parvovirus Infection was 94.1%; the specificity was 93.3%; the positive and negative predictive values were 94.1% and 93.3%, respectively. Conclusion: Fetal anemia caused by Parvovirus Infection can be detected noninvasively by Doppler ultrasonography on the basis of an increase in the peak velocity of systolic blood flow in the middle cerebral artery. (Am J Obstet Gynecol 2002;187:1290-3.)
Lisa Goldstein - One of the best experts on this subject based on the ideXlab platform.
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Retrospective diagnosis of sickle cell-hemoglobin C disease and Parvovirus Infection by molecular DNA analysis of postmortem tissue
Human Pathology, 1995Co-Authors: Lisa Goldstein, Rochelle Strenger, Stacy Comfort Le, Thomas King, Beverly Barton RogersAbstract:Abstract A previously healthy 30-year-old African-American woman presented with a history of sickle cell trait and a nonspecific prodromal illness with severe bone pain. She experienced rapid clinical deterioration with seizures and cardiorespiratory arrest leading to death. Autopsy showed necrotic bone marrow with extensive bone marrow emboli. Parvovirus Infection was documented by polymerase chain reaction (PCR) although diagnostic intranuclear inclusions or giant pronormoblasts were not present. The diagnosis of sickle cell-hemoglobin C disease (hemoglobin SC disease) was also established postmortem by DNA sequencing of PCR products. This case illustrates the use and versatility of PCR for analysis of formalin-fixed paraffin-embedded autopsy tissue.
Stacy Comfort Le - One of the best experts on this subject based on the ideXlab platform.
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Retrospective diagnosis of sickle cell-hemoglobin C disease and Parvovirus Infection by molecular DNA analysis of postmortem tissue
Human Pathology, 1995Co-Authors: Lisa Goldstein, Rochelle Strenger, Stacy Comfort Le, Thomas King, Beverly Barton RogersAbstract:Abstract A previously healthy 30-year-old African-American woman presented with a history of sickle cell trait and a nonspecific prodromal illness with severe bone pain. She experienced rapid clinical deterioration with seizures and cardiorespiratory arrest leading to death. Autopsy showed necrotic bone marrow with extensive bone marrow emboli. Parvovirus Infection was documented by polymerase chain reaction (PCR) although diagnostic intranuclear inclusions or giant pronormoblasts were not present. The diagnosis of sickle cell-hemoglobin C disease (hemoglobin SC disease) was also established postmortem by DNA sequencing of PCR products. This case illustrates the use and versatility of PCR for analysis of formalin-fixed paraffin-embedded autopsy tissue.
Rochelle Strenger - One of the best experts on this subject based on the ideXlab platform.
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Retrospective diagnosis of sickle cell-hemoglobin C disease and Parvovirus Infection by molecular DNA analysis of postmortem tissue
Human Pathology, 1995Co-Authors: Lisa Goldstein, Rochelle Strenger, Stacy Comfort Le, Thomas King, Beverly Barton RogersAbstract:Abstract A previously healthy 30-year-old African-American woman presented with a history of sickle cell trait and a nonspecific prodromal illness with severe bone pain. She experienced rapid clinical deterioration with seizures and cardiorespiratory arrest leading to death. Autopsy showed necrotic bone marrow with extensive bone marrow emboli. Parvovirus Infection was documented by polymerase chain reaction (PCR) although diagnostic intranuclear inclusions or giant pronormoblasts were not present. The diagnosis of sickle cell-hemoglobin C disease (hemoglobin SC disease) was also established postmortem by DNA sequencing of PCR products. This case illustrates the use and versatility of PCR for analysis of formalin-fixed paraffin-embedded autopsy tissue.