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Yuhana Munti - One of the best experts on this subject based on the ideXlab platform.
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Existing of White Spot Syndrome Virus (WSSV), Taura Syndrome Virus (TSV) and Infectious Hypodermal Haematopoitic Necrosis Virus (IHHNV) in White Shrimp Litopenaeus vannamei Reared at Intensive Tambak System in Bakauheni, Lampung Selatan
'Jurnal Akuakultur Indonesia', 2009Co-Authors: Dwinanti S.h., Yuhana MuntiAbstract:Development of white shrimp, Litopenaeus vannamei culture to increase production yield should take attention in prevention of viral outbreak which is a main factor caused harvesting failure. Viruses that usually infect shrimp are white spot syndrome virus (WSSV), taura syndrome virus (TSV) dan infectious hypodermal hematopoietic necrosis virus (IHHNV). To prevent virus infection and reduce risk of fail to harvest, an early warning and observation system of availability of pathogen should be taken in order to know the shrimp condition during culture process. This study was performed to examine existing of WSSV, TSV and IHHNV in shrimp reared in tambak by PCR and histopathology methods. Samples were taken every two weeks from tambak culture at Bakauheni, Penengahan, Lampung Selatan. Generally, WSSV, TSV, and IHHNV were found in shrimp from tambak and water around tambak. Virus infection was first detected in shrimp derived from tambak on 66 days after crop and still exists until 107 days after crop. Hepatopancreatic cell disorder caused by the three viruses were found in cell nuclei that became bigger, necrosis in cytoplasm and inclusion body at nuclei. The existing of virus in outside of tambak indicated that virus was horizontally transmitted. Keywords: WSSV, TSV, IHHNV, white shrimp ABSTRAK Perkembangan budidaya udang vaname Litopenaeus vannamei dalam usaha meningkatkan hasil produksi harus memperhatikan keberadaan penyakit viral yang menjadi penyebab utama kegagalannya. Virus yang biasa menyerang vaname antara lain white spot syndrome virus (WSSV), taura syndrome virus (TSV) dan infectious hypodermal hematopoietic necrosis virus (IHHNV). Untuk mengantisipasi penyebaran virus dan mengurangi resiko kegagalan produksi diperlukan usaha pencegahan dengan melakukan peringatan dini (early warning) dan pemantauan tambak terhadap keberadaan patogen tersebut selama masa budidaya. Penelitian ini dilakukan untuk mengetahui keberadaan WSSV, TSV dan IHHNV yang menginfeksi udang vaname dengan analisa polymerase chain reaction (PCR) dan histopatologis. Pengambilan sampel dilakukan sebanyak delapan kali setiap dua minggu di tambak intensif udang vaname Litopenaeus vannamei di Bakauheni, Penengahan, Lampung Selatan. Secara umum, WSSV, TSV dan IHHNV telah ditemukan pada tambak serta di perairan sekitar lokasi tersebut. Infeksi virus pertama kali terdeteksi di tambak ketika udang berumur 66 hari setelah penebaran dan terdeteksi pula pada waktu pengamatan selanjutnya sampai udang berumur 107 hari setelah penebaran. Kerusakan akibat serangan ketiga jenis virus tersebut terjadi pada bagian inti sel yang mengalami pembesaran, nekrosis pada sitoplasma dan badan inklusi yang menekan inti sel. Pola transmisi virus yang terjadi adalah secara horizontal yang didukung oleh terdeteksinya virus tersebut pada udang di luar tambak. Kata kunci : WSSV, TSV, IHHNV, udang vanam
Munti Yuhana - One of the best experts on this subject based on the ideXlab platform.
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existing of white spot syndrome virus wssv taura syndrome virus tsv and infectious hypodermal haematopoitic necrosis virus ihhnv in white shrimp litopenaeus vannamei reared at intensive tambak system in bakauheni lampung selatan
Jurnal Akuakultur Indonesia, 2009Co-Authors: Sefti Heza Dwinanti, Munti YuhanaAbstract:Development of white shrimp, Litopenaeus vannamei culture to increase production yield should take attention in prevention of viral outbreak which is a main factor caused harvesting failure. Viruses that usually infect shrimp are white spo t syndrome vi r u s (WSSV), taura syndrome virus (TSV) dan infectious hypodermal hematopoietic necrosis v iru s (IHHNV). To prevent virus infection and reduce risk of fail to harvest, an early warning and observation system of availability of pathogen should be taken in order to know the shrimp condition during culture process. This study was performed to examine existing of WSSV, TSV and IHHNV in shrimp reared in tambak by PCR and histopathology methods. Samples were taken every two weeks from tambak culture at Bakauheni, Penengahan, Lampung Selatan. Generally, WSSV, TSV, and IHHNV were found in shrimp from tambak and water around tambak. Virus infection was first detected in shrimp derived from tambak on 66 days after crop and still exists until 107 days after crop. Hepatopancreatic cell disorder caused by the three viruses were found in cell nuclei that became bigger, necrosis in cytoplasm and inclusion body at nuclei. The existing of virus in outside of tambak indicated that virus was horizontally transmitted. Keywords: WSSV, TSV, IHHNV, white shrimp ABSTRAK Perkembangan budidaya udang vaname Litopenaeus vannamei dalam usaha meningkatkan hasil produksi harus memperhatikan keberadaan penyakit viral yang menjadi penyebab utama kegagalannya. Virus yang biasa menyerang vaname antara lain white spot syndrome virus (WSSV), taura syndrome virus (TSV) dan infectious hypodermal hematopoietic necrosis virus (IHHNV). Untuk mengantisipasi penyebaran virus dan mengurangi resiko kegagalan produksi diperlukan usaha pencegahan dengan melakukan peringatan dini ( early warning ) dan pemantauan tambak terhadap keberadaan patogen tersebut selama masa budidaya. Penelitian ini dilakukan untuk mengetahui keberadaan WSSV, TSV dan IHHNV yang menginfeksi udang vaname dengan analisa polymerase chain reaction (PCR) dan histopatologis. Pengambilan sampel dilakukan sebanyak delapan kali setiap dua minggu di tambak intensif udang vaname Litopenaeus vannamei di Bakauheni, Penengahan, Lampung Selatan. Secara umum, WSSV, TSV dan IHHNV telah ditemukan pada tambak serta di perairan sekitar lokasi tersebut. Infeksi virus pertama kali terdeteksi di tambak ketika udang berumur 66 hari setelah penebaran dan terdeteksi pula pada waktu pengamatan selanjutnya sampai udang berumur 107 hari setelah penebaran. Kerusakan akibat serangan ketiga jenis virus tersebut terjadi pada bagian inti sel yang mengalami pembesaran, nekrosis pada sitoplasma dan badan inklusi yang menekan inti sel. Pola transmisi virus yang terjadi adalah secara horizontal yang didukung oleh terdeteksinya virus tersebut pada udang di luar tambak. Kata kunci : WSSV, TSV, IHHNV, udang vaname
Cicek B - One of the best experts on this subject based on the ideXlab platform.
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The cytotoxic and genotoxic effects of daidzein on MIA PaCa-2 human pancreatic carcinoma cells and HT-29 human colon cancer cells.
'Informa UK Limited', 2020Co-Authors: Gundogdu G, Dodurga Y, Cetin M, Secme M, Cicek BAbstract:Daidzein (DZ) has anti-inflammatory and antioxidant effects, as well as the dose-dependent inhibition effect on cancer cells. In this study, the cytotoxic and genotoxic effects of DZ on HT-29 (human colorectal adenocarcinoma cells) and MIA PaCa-2 (human pancreatic cancer cells) cell lines were determined using the XTT method and Comet assay, respectively. IC(50) concentrations of DZ were found to be 200 µM in both MIA PaCa-2 and HT-29 cells treated with DZ for 48 hours (h). When the cells were treated with 200 μM of DZ for 48 h, DNA damage was observed in both cell lines. DNA tail length (TL), tail moment (TM), and tail intensity (TI) increased more in MIA PaCa-2 cells treated with 200 μM of DZ than those in the control cell (untreated MIA PaCa-2 cell) group (p 
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The cytotoxic and genotoxic effects of daidzein on MIA PaCa-2 human pancreatic carcinoma cells and HT-29 human colon cancer cells
'Informa UK Limited', 2020Co-Authors: Gundogdu G, Dodurga Y, Cetin M, Secme M, Cicek BAbstract:Daidzein (DZ) has anti-inflammatory and antioxidant effects, as well as the dose-dependent inhibition effect on cancer cells. In this study, the cytotoxic and genotoxic effects of DZ on HT-29 (human colorectal adenocarcinoma cells) and MIA PaCa-2 (human pancreatic cancer cells) cell lines were determined using the XTT method and Comet assay, respectively. IC50 concentrations of DZ were found to be 200 µM in both MIA PaCa-2 and HT-29 cells treated with DZ for 48 hours (h). When the cells were treated with 200 μM of DZ for 48 h, DNA damage was observed in both cell lines. DNA tail length (TL), tail moment (TM), and tail intensity (TI) increased more in MIA PaCa-2 cells treated with 200 μM of DZ than those in the control cell (untreated MIA PaCa-2 cell) group (p < 0.01). However, only DNA-TI and DNA-TM exhibited higher increases in HT-29 cells treated with 200 μM of DZ than those in the control cell (untreated HT-29 cell) group (p < 0.01). This shows that DZ has cytotoxic and genotoxic effects on both cell lines. The observed genotoxic effects of DZ still need to be confirmed in additional future studies. © 2018 Informa UK Limited, trading as Taylor & Francis Group
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pancreatic carcinoma cells and HT-29 human colon cancer cells
'Informa UK Limited', 2020Co-Authors: Gundogdu G, Dodurga Y, Cetin M, Secme M, Cicek BAbstract:Daidzein (DZ) has anti-inflammatory and antioxidant effects, as well as the dose-dependent inhibition effect on cancer cells. In this study, the cytotoxic and genotoxic effects of DZ on HT-29 (human colorectal adenocarcinoma cells) and MIA PaCa-2 (human pancreatic cancer cells) cell lines were determined using the XTT method and Comet assay, respectively. IC(50)concentrations of DZ were found to be 200 mu M in both MIA PaCa-2 and HT-29 cells treated with DZ for 48 hours (h). When the cells were treated with 200 mu M of DZ for 48 h, DNA damage was observed in both cell lines. DNA tail length (TL), tail moment (TM), and tail intensity (TI) increased more in MIA PaCa-2 cells treated with 200 mu M of DZ than those in the control cell (untreated MIA PaCa-2 cell) group (p < 0.01). However, only DNA-TI and DNA-TM exhibited higher increases in HT-29 cells treated with 200 mu M of DZ than those in the control cell (untreated HT-29 cell) group (p < 0.01). This shows that DZ has cytotoxic and genotoxic effects on both cell lines. The observed genotoxic effects of DZ still need to be confirmed in additional future studies.C1 [Gundogdu, Gulsah; Cicek, Betul] Ataturk Univ, Dept Physiol, Fac Med, Erzurum, Turkey.[Dodurga, Yavuz; Secme, Mucahit] Pamukkale Univ, Fac Med, Dept Med Biol, Denizli, Turkey.[Cetin, Meltem] Ataturk Univ, Fac Pharm, Dept Pharmaceut Technol, Erzurum, Turkey
Catharina De Lange Davies - One of the best experts on this subject based on the ideXlab platform.
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cellular uptake and intracellular degradation of poly alkyl cyanoacrylate nanoparticles
Journal of Nanobiotechnology, 2016Co-Authors: Einar Sulheim, Yrr Mørch, Habib Baghirov, Eva Von Haartman, Andreas Boe, Andreas Aslund, Catharina De Lange DaviesAbstract:Poly(alkyl cyanoacrylate) (PACA) nanoparticles have shown promise as drug carriers both to solid tumors and across the blood–brain barrier. Efficient drug delivery requires both high cellular uptake of the nanoparticles and release of the drug from the nanoparticles. Release of hydrophobic drugs from PACA nanoparticles is primarily governed by nanoparticle degradation, and this process has been poorly studied at the cellular level. Here we use the hydrophobic model drug Nile Red 668 (NR668) to investigate intracellular degradation of PACA nanoparticles by measuring changes in NR668 fluorescence emission and lifetime, as the spectral properties of NR668 depend on the hydrophobicity of the dye environment. We also assess the potential of poly(butyl cyanoacrylate) (PBCA) and poly(octyl cyanoacrylate) (POCA) nanoparticles for intracellular drug delivery in the prostate cancer cell line PC3 and rat brain endothelial cell line RBE4 and the role of endocytosis pathways in PACA nanoparticle uptake in those cell lines. Fluorescence lifetime imaging, emission spectra analysis and Forster resonance energy transfer indicated that the intracellular degradation was in line with the degradation found by direct methods such as gas chromatography and scanning electron microscopy, showing that PBCA has a faster degradation rate compared to POCA. The combined P(BCA/OCA) nanoparticles had an intermediate degradation rate. The uptake of POCA and PBCA nanoparticles was much higher in RBE4 than in PC3 cells. Endocytosis inhibition studies showed that both clathrin- and caveolin-mediated endocytosis were involved in PACA nanoparticle uptake, and that the former played a predominant role, particularly in PC3 cells. In the present study, we used three different optical techniques to show that within a 24-hour period PBCA nanoparticles degraded significantly inside cells, releasing their payload into the cytosol, while POCA nanoparticles remained intact. This indicates that it is possible to tune the intracellular drug release rate by choosing appropriate monomers from the PACA family or by using hybrid PACA nanoparticles containing different monomers. In addition, we showed that the uptake of PACA nanoparticles depends not only on the monomer material, but also on the cell type, and that different cell lines can use different internalization pathways.
Eva Von Haartman - One of the best experts on this subject based on the ideXlab platform.
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cellular uptake and intracellular degradation of poly alkyl cyanoacrylate nanoparticles
Journal of Nanobiotechnology, 2016Co-Authors: Einar Sulheim, Yrr Mørch, Habib Baghirov, Eva Von Haartman, Andreas Boe, Andreas Aslund, Catharina De Lange DaviesAbstract:Poly(alkyl cyanoacrylate) (PACA) nanoparticles have shown promise as drug carriers both to solid tumors and across the blood–brain barrier. Efficient drug delivery requires both high cellular uptake of the nanoparticles and release of the drug from the nanoparticles. Release of hydrophobic drugs from PACA nanoparticles is primarily governed by nanoparticle degradation, and this process has been poorly studied at the cellular level. Here we use the hydrophobic model drug Nile Red 668 (NR668) to investigate intracellular degradation of PACA nanoparticles by measuring changes in NR668 fluorescence emission and lifetime, as the spectral properties of NR668 depend on the hydrophobicity of the dye environment. We also assess the potential of poly(butyl cyanoacrylate) (PBCA) and poly(octyl cyanoacrylate) (POCA) nanoparticles for intracellular drug delivery in the prostate cancer cell line PC3 and rat brain endothelial cell line RBE4 and the role of endocytosis pathways in PACA nanoparticle uptake in those cell lines. Fluorescence lifetime imaging, emission spectra analysis and Forster resonance energy transfer indicated that the intracellular degradation was in line with the degradation found by direct methods such as gas chromatography and scanning electron microscopy, showing that PBCA has a faster degradation rate compared to POCA. The combined P(BCA/OCA) nanoparticles had an intermediate degradation rate. The uptake of POCA and PBCA nanoparticles was much higher in RBE4 than in PC3 cells. Endocytosis inhibition studies showed that both clathrin- and caveolin-mediated endocytosis were involved in PACA nanoparticle uptake, and that the former played a predominant role, particularly in PC3 cells. In the present study, we used three different optical techniques to show that within a 24-hour period PBCA nanoparticles degraded significantly inside cells, releasing their payload into the cytosol, while POCA nanoparticles remained intact. This indicates that it is possible to tune the intracellular drug release rate by choosing appropriate monomers from the PACA family or by using hybrid PACA nanoparticles containing different monomers. In addition, we showed that the uptake of PACA nanoparticles depends not only on the monomer material, but also on the cell type, and that different cell lines can use different internalization pathways.