The Experts below are selected from a list of 36825 Experts worldwide ranked by ideXlab platform
Delphine Arcizet - One of the best experts on this subject based on the ideXlab platform.
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temporal analysis of active and Passive Transport in living cells
Physical Review Letters, 2008Co-Authors: Delphine Arcizet, Born Meier, Erich Sackmann, Joachim O Radler, Doris HeinrichAbstract:The cellular cytoskeleton is a fascinating active network, in which Brownian motion is intercepted by distinct phases of active Transport. We present a time-resolved statistical analysis dissecting phases of directed motion out of otherwise diffusive motion of tracer particles in living cells. The distribution of active lifetimes is found to decay exponentially with a characteristic time tauA = 0.65 s. The velocity distribution of active events exhibits several peaks, in agreement with a discrete number of motor proteins acting collectively.
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temporal analysis of active and Passive Transport in living cells
Physical Review Letters, 2008Co-Authors: Delphine Arcizet, Born Meier, Erich Sackmann, Joachim O Radler, Doris HeinrichAbstract:The cellular cytoskeleton is a fascinating active network, in which Brownian motion is intercepted by distinct phases of active Transport. We present a time-resolved statistical analysis dissecting phases of directed motion out of otherwise diffusive motion of tracer particles in living cells. The distribution of active lifetimes is found to decay exponentially with a characteristic time ${\overline{\ensuremath{\tau}}}_{A}=0.65\text{ }\text{ }\mathrm{s}$. The velocity distribution of active events exhibits several peaks, in agreement with a discrete number of motor proteins acting collectively.
Doris Heinrich - One of the best experts on this subject based on the ideXlab platform.
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temporal analysis of active and Passive Transport in living cells
Physical Review Letters, 2008Co-Authors: Delphine Arcizet, Born Meier, Erich Sackmann, Joachim O Radler, Doris HeinrichAbstract:The cellular cytoskeleton is a fascinating active network, in which Brownian motion is intercepted by distinct phases of active Transport. We present a time-resolved statistical analysis dissecting phases of directed motion out of otherwise diffusive motion of tracer particles in living cells. The distribution of active lifetimes is found to decay exponentially with a characteristic time tauA = 0.65 s. The velocity distribution of active events exhibits several peaks, in agreement with a discrete number of motor proteins acting collectively.
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temporal analysis of active and Passive Transport in living cells
Physical Review Letters, 2008Co-Authors: Delphine Arcizet, Born Meier, Erich Sackmann, Joachim O Radler, Doris HeinrichAbstract:The cellular cytoskeleton is a fascinating active network, in which Brownian motion is intercepted by distinct phases of active Transport. We present a time-resolved statistical analysis dissecting phases of directed motion out of otherwise diffusive motion of tracer particles in living cells. The distribution of active lifetimes is found to decay exponentially with a characteristic time ${\overline{\ensuremath{\tau}}}_{A}=0.65\text{ }\text{ }\mathrm{s}$. The velocity distribution of active events exhibits several peaks, in agreement with a discrete number of motor proteins acting collectively.
Martin B Ulmschneider - One of the best experts on this subject based on the ideXlab platform.
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molecular dynamics simulation of Passive Transport of neurotoxicant antidotes through the blood brain barrier
Biophysical Journal, 2016Co-Authors: Yukun Wang, Peter C Searson, Martin B UlmschneiderAbstract:Systemic delivery of neurotoxicant antidotes into the brain is controlled by the blood-brain barrier. At present the atomic detail mechanisms of molecular Transport across the blood brain barrier are poorly understood, hindering optimization of delivery properties of next-generation antidotes. Here we study the mechanisms of Passive molecular Transport of a number of oximes and other small molecules across the blood-brain barrier. For this purpose, we have built atomic detail molecular models of the apical and basolateral lipid bilayer membranes of human brain microvascular endothelial cells. Lipid bilayer properties were validated against experimental data. Subsequently the diffusive Transport of oximes and other small molecules across the apical and basolateral lipid bilayer was simulated using atomic detail molecular dynamics simulations. This allowed measurement of transbilayer permeation rates, which can be directly compared to experimental data, as well as providing atomic detail insights into the mechanisms of drug Transport. The simulations reveal how individual drug chemistry (e.g. lipophilicity, polarity, hydrogen bonds) affects drug-bilayer interactions, and ultimately the rate of Passive diffusion across the bilayer.
Carmen Wangler - One of the best experts on this subject based on the ideXlab platform.
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design of brain imaging agents for positron emission tomography do large bioconjugates provide an opportunity for in vivo brain imaging
Future Medicinal Chemistry, 2013Co-Authors: Ralf Schirrmacher, Vadim Bernardgauthier, Andrew J Reader, Jeanpaul Soucy, Esther Schirrmacher, Bjorn Wangler, Carmen WanglerAbstract:The development of brain imaging agents for positron emission tomography and other in vivo imaging modalities mostly relies on small compounds of low MW as a result of the restricted Transport of larger molecules, such as peptides and proteins, across the blood–brain barrier. Besides Passive Transport, only a few active carrier mechanisms, such as glucose Transporters and amino acid Transporters, have so far been exploited to mediate the accumulation of imaging probes in the brain. An important question for the future is whether some of the abundant active carrier systems located at the blood–brain barrier can be used to shuttle potential, but non-crossing, imaging agents into the brain. What are the biological and chemical constrictions toward such bioconjugates and is it worthwhile to persue such a delivery strategy?
Christian Amatore - One of the best experts on this subject based on the ideXlab platform.
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Monitoring and quantifying the Passive Transport of molecules through patch-clamp suspended real and model cell membranes.
Angewandte Chemie International Edition, 2014Co-Authors: Pierluca Messina, Frédéric Lemaître, François Huet, Kieu Ngo, Vincent Vivier, Eric Labbé, Olivier Buriez, Christian AmatoreAbstract:Transport of active molecules across biological membranes is a central issue for the success of many pharmaceutical strategies. Herein, we combine the patch-clamp principle with amperometric detection for monitoring fluxes of redox-tagged molecular species across a suspended membrane patched from a macrophage. Solvent- and protein-free lipid bilayers (DPhPC, DOPC, DOPG) patched from single-wall GUV have been thoroughly investigated and the corresponding fluxes measurements quantified. The quality of the patches and their proper sealing were successfully characterized by electrochemical impedance spectroscopy. This procedure appears versatile and perfectly adequate to allow the investigation of Transport and quantification of the Transport properties through direct measurement of the coefficients of partition and diffusion of the compound in the membrane, thus offering insight on such important biological and pharmacological issues.