The Experts below are selected from a list of 219 Experts worldwide ranked by ideXlab platform
R.b Williams - One of the best experts on this subject based on the ideXlab platform.
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A self-filling microPipette for the isolation of single coccidial oocysts
Research in Veterinary Science, 1997Co-Authors: R.b WilliamsAbstract:Abstract A disposable, self-filling microPipette is described for the isolation of single oocysts of chicken coccidia from laboratory stocks. A sterile 1 μl capillary tube is mounted, using sealing wax, into the narrower end of a broken-off Pasteur Pipette. This microPipette is pushed into one end of a 60 cm length of flexible plastic tubing and a 5 cm glass tube is pushed into the other end. The apparatus is used by holding the glass tube in one's mouth, maintaining a positive pressure in the buccal cavity, while the tip of the capillary tube in the microPipette is juxtaposed with a single oocyst in a diluted stock on a cavity slide, observed under a microscope objective. A sudden release of the positive pressure causes the chosen oocyst to be instantaneously taken up into the capillary tube. Using the tube again, the oocyst is then blown out onto a piece of ‘Cellophane’ which is placed in a gelatine capsule. The capsule is administered to a chick in a plastic-film isolator.
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A self-filling microPipette for the isolation of single coccidial oocysts
Research in veterinary science, 1997Co-Authors: R.b WilliamsAbstract:A disposable, self-filling microPipette is described for the isolation of single oocysts of chicken coccidia from laboratory stocks. A sterile 1 microliter capillary tube is mounted, using sealing wax, into the narrower end of a broken-off Pasteur Pipette. This microPipette is pushed into one end of a 60 cm length of flexible plastic tubing and a 5 cm glass tube is pushed into the other end. The apparatus is used by holding the glass tube in one's mouth, maintaining a positive pressure in the buccal cavity, while the tip of the capillary tube in the microPipette is juxtaposed with a single oocyst in a diluted stock on a cavity slide, observed under a microscope objective. A sudden release of the positive pressure causes the chosen oocyst to be instantaneously taken up into the capillary tube. Using the tube again, the oocyst is then blown out onto a piece of 'Cellophane' which is placed in a gelatine capsule. The capsule is administered to a chick in a plastic-film isolator.
Toshiki Miyazaki - One of the best experts on this subject based on the ideXlab platform.
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Preparation of porous yttrium oxide microparticles by gelation of ammonium alginate in aqueous solution containing yttrium ions
Journal of materials science. Materials in medicine, 2010Co-Authors: Masakazu Kawashita, Naoko Matsui, Toshiki MiyazakiAbstract:Porous Y2O3 microparticles 500 microm in size were obtained, when 1 wt%-ammonium alginate aqueous solution was dropped into 0.5 M-YCl3 aqueous solution by a Pasteur Pipette and the resultant gel microparticles were heat-treated at 1100 degrees C. Small pores less than 1 microm were formed in the microparticles by the heat treatment. The bulk density of the heat-treated microparticle was as low as 0.66 g cm(-3). The chemical durability of the heat-treated microparticles in simulated body fluid at pH = 6 and 7 was high enough for clinical application of in situ radiotherapy. Although the size of the microparticles should be decreased to around 25 microm using atomizing device such as spray gun for clinical application, we found that the porous Y2O3 microparticles with high chemical durability and low density can be obtained by utilizing gelation of ammonium alginate in YCl3 aqueous solution in this study.
Masakazu Kawashita - One of the best experts on this subject based on the ideXlab platform.
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Preparation of porous yttrium oxide microparticles by gelation of ammonium alginate in aqueous solution containing yttrium ions
Journal of materials science. Materials in medicine, 2010Co-Authors: Masakazu Kawashita, Naoko Matsui, Toshiki MiyazakiAbstract:Porous Y2O3 microparticles 500 microm in size were obtained, when 1 wt%-ammonium alginate aqueous solution was dropped into 0.5 M-YCl3 aqueous solution by a Pasteur Pipette and the resultant gel microparticles were heat-treated at 1100 degrees C. Small pores less than 1 microm were formed in the microparticles by the heat treatment. The bulk density of the heat-treated microparticle was as low as 0.66 g cm(-3). The chemical durability of the heat-treated microparticles in simulated body fluid at pH = 6 and 7 was high enough for clinical application of in situ radiotherapy. Although the size of the microparticles should be decreased to around 25 microm using atomizing device such as spray gun for clinical application, we found that the porous Y2O3 microparticles with high chemical durability and low density can be obtained by utilizing gelation of ammonium alginate in YCl3 aqueous solution in this study.
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Enzymatic preparation of hollow magnetite microspheres for hyperthermic treatment of cancer.
Journal of materials science. Materials in medicine, 2006Co-Authors: Masakazu Kawashita, Kazuo Sadaoka, Tadashi Kokubo, Takashi Saito, Mikio Takano, Norio Araki, Masahiro HiraokaAbstract:Ferrimagnetic materials can be expected to be useful as thermal seeds for hyperthermic treatment of cancer, especially where the cancer is located in deep parts of body, as they can generate heat by magnetic hysteretic loss when they are placed in an alternating magnetic field. In this study, hollow magnetite (Fe3O4) particles were prepared using an enzymatic reaction of urease. A hollow particle was obtained by using a Pasteur Pipette. The particle was 500 μm in size and was composed of Fe3O4. Its saturation magnetization and coercive force were 57 emu⋅g−1 and 183 Oe, respectively. Its heat generation under an alternating magnetic field of 300 Oe at 100 kHz was estimated to be 45 W⋅g−1. Microspheres 30 μm in diameter were also successfully obtained by using a spray gun.
Mohammad Hossein Nasr-esfahani - One of the best experts on this subject based on the ideXlab platform.
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A Novel Method of Somatic Cell Nuclear Transfer with Minimum Equipment.
Methods in molecular biology (Clifton N.J.), 2015Co-Authors: S.m. Hosseini, F. Moulavi, Mohammad Hossein Nasr-esfahaniAbstract:Somatic cell nuclear transfer (SCNT) is an exceptional experimental biology technique with an arguably great contribution to our current understanding of developmental plasticity. Many students and young researchers are interested in taking advantage of SCNT virtues in their experiments but the cost of micromanipulation microscopes, intensive training programs, and also the sophisticated process of SCNT may dissuade them from entering this amazing field of science. Here, we describe the details of a streamlined manual method of SCNT that can be performed using very basic equipment found in every embryology laboratory: the Pasteur Pipette and stereomicroscope. The overall method introduced is very simple and a person with no previous experience in cloning can learn and adopt the basic routines of this technique independently.
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Simple, fast, and efficient method of manual oocyte enucleation using a pulled Pasteur Pipette
In Vitro Cellular & Developmental Biology - Animal, 2013Co-Authors: S.m. Hosseini, F. Moulavi, V. Asgari, A. Shirazi, A. H. Abazari-kia, H. R. Ghanaei, Mohammad Hossein Nasr-esfahaniAbstract:Cloning mammals by somatic cell nuclear transfer entails the replacement of oocyte chromosomes with the nucleus of a somatic cell. A major step in this technique is to efficiently produce large batches of enucleated oocytes, a process that requires considerable micromanipulation skills and expensive equipments. Here, a simple, fast, and efficient method of manual oocyte enucleation was introduced that can be adopted in every laboratory with the minimum equipments. Common laboratory glass Pipettes were pulled on the flame of a burner and then used for manual bisection or enucleation of sheep and goat zona-free oocytes by passing them through the discontinuous cutting border of culture medium and mineral oil. The described techniques showed a certain efficiency to conveniently bisect or enucleate large batches of sheep, and goat oocytes being pre-treated with demecolcine. The method may be straightforward for simple manipulation of oocytes of other species and for development of automated cloning methods as well.
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Cloned Sheep Blastocysts Derived from Oocytes Enucleated Manually Using a Pulled Pasteur Pipette
Cellular reprogramming, 2013Co-Authors: S.m. Hosseini, Hajian, F. Moulavi, V. Asgari, M. Forouzanfar, Mohammad Hossein Nasr-esfahaniAbstract:Abstract The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulation skill and expensive equipment. Here, we have developed an efficient and high-throughput method of manual oocyte enucleation using a simple device, a pulled Pasteur Pipette, that can be connected to standard zona-free method of embryo reconstitution. Common Pasteur Pipettes were pulled on a flame to produce finely drawn Pipettes with inner diameters approximately less than half the oocyte diameter (∼50–60 μm), and slightly larger than cytoplasmic protrusion (∼20–30 μm) that was induced after demecolcine treatment of MII-stage oocytes. Oocyte manipulation was performed under a stereomicroscope by either bisecting the oocyte into two approximately equal demioocytes (blind manual enucl...
Isabelle Vernos - One of the best experts on this subject based on the ideXlab platform.
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In vitro Assays for Mitotic Spindle Assembly and Function
Cell Biology, 2006Co-Authors: Celia Antonio, Rebecca Heald, Isabelle VernosAbstract:Publisher Summary This chapter focuses on in vitro assays for mitotic spindle assembly and function. Preparation of X. laevis CSF egg extracts involves dissolving in sterile water to a final concentration of 200 units/ml. Cut off the end of a glass Pasteur Pipette and fire polish it to make a wide-mouth Pipette. Collect laid eggs in MMR. Frogs can also be squeezed, which often gives the highest quality eggs. For preparation of DNA beads, linearized couple plasmid DNA and fill in with nucleotides so that one ends contains biotinylated bases and the other thionucleotides to inhibit exonuclease activity and the formation of large aggregates of beads. Prepare plasmid DNA by Qiagen column purification. While the sequence of the DNA is not important, the plasmid should be more than 5 kb to effectively induce chromatin assembly. Each sperm nucleus is tightly associated with a centriole. In a very simple process, half spindles can assemble around sperm nuclei just by incubation of the sperm nuclei in the extract.