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Veronika Bártová - One of the best experts on this subject based on the ideXlab platform.
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Antifungal and antimicrobial proteins and peptides of potato (Solanum tuberosum L.) tubers and their applications
Applied Microbiology and Biotechnology, 2019Co-Authors: Veronika Bártová, Jan Bárta, Markéta JarošováAbstract:Potato proteins are well known for their nutritional, emulsifying, foaming, gel forming or antioxidant properties that all make from them valuable protein source for food industry. Antifungal, antimicrobial and also antiviral properties, described for potato proteins in the review, enrich the possibilities of potato protein usage. Potato proteins were divided into Patatin, protease inhibitors and fraction of other proteins that also included, besides others, proteins involved in potato defence physiology. All these proteins groups provide proteins and peptides with antifungal and/or antimicrobial actions. Patatins, obtained from cultivars with resistance to Phytophthora infestans , were able to inhibit spore germination of this pathogen. Protease inhibitors represent the structurally heterogeneous group with broad range of antifungal and antimicrobial activities. Potato protease inhibitors I and II reduced the growth of Phytophthora infestans , Rhizoctonia solani and Botrytis cinerea or of the fungi of Fusarium genus. Members of Kunitz family (proteins Potide-G, AFP-J, Potamin-1 or PG-2) were able to inhibit serious pathogens such as Staphylococcus aureus , Listeria monocytogenes , Escherichia coli or Candida albicans. Potato snakins, defensins and pseudothionins are discussed for their ability to inhibit serious potato fungi as well as bacterial pathogens. Potato proteins with the ability to inhibit growth of pathogens were used for developing of pathogen-resistant transgenic plants for crop improvement. Incorporation of potato antifungal and antimicrobial proteins in feed and food products or food packages for elimination of hygienically risk pathogens brings new possibility of potato protein usage.
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Patatin the major protein of potato solanum tuberosum l tubers and its occurrence as genotype effect processing versus table potatoes
Czech Journal of Food Sciences, 2018Co-Authors: Jan Bárta, Veronika BártováAbstract:Patatin relative abundance in SDS-extractable protein and Patatin content in dry matter were evaluated in tubers of forty processing and table potato cultivars usually cultivated in the Czech Republic, Germany, and the Netherlands. The Patatin characteristics were evaluated over three experimental years. Patatin relative abundance in the processing cultivars achieved on average a significantly higher value (P < 0.001; Tukey HSD test) than Patatin relative abundance in the table cultivars, resulting in average values of 25.80% and 21.59%, respectively. A high Patatin relative abundance (over 30% in extractable protein) was determined only in the case of two cultivars: Vaneda (average 31.29%) and Tomensa (average 31.24%). Patatin content in tuber dry matter was significantly higher in the processing potato cultivars in all three experimental years (P < 0.001), attaining a mean of 1.28% with the processing cultivars and 1.03% with the table cultivars. The direct effect of the cultivar on Patatin relative abundance in SDS-extractable protein was higher (33.1% for processing potato cultivars and 48.1% for table potato cultivars) than the effect of the growing year (15.6% for processing potato cultivar and 22.8% for table potato cultivars).
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amino acid composition and nutritional value of four cultivated south american potato species
Journal of Food Composition and Analysis, 2015Co-Authors: Veronika Bártová, Jan Bárta, Adela Brabcova, Zbyněk Zdrahal, Vendulka HorackovaAbstract:Protein content, amino acid composition, nutritional quality and Patatin characteristics were determined in tubers of four South American cultivated potato species (Solanum andigenum, Solanum goniocalyx, Solanum phureja, Solanum stenotomum) and a cultivar of the commonly cultivated cultivar Desiree of Solanum tuberosum. Protein content (on dry matter basis) of S. andigenum and S. stenotomum was highest at 7.9% and 8.0%, respectively, and the relative quantities of Patatin for both species were 41.7% and 34.0%, respectively. The nutritional value of Patatin, in terms of essential amino acid index with respect to a reference protein of FAO/WHO (EAAI(adult)) ranged from 93.0% (S. phureja) to 112.5% (S. goniocalyx). In case S. goniocalyx, the Patatin fraction was a nutritionally better protein fraction than a protein concentrate or tuber dry matter, which had EAAI(adult) values of 97.6% and 82.9%, respectively. This suggests that this species may have potential in potato breeding programmes and in human nutrition.
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cultivar variability of Patatin biochemical characteristics table versus processing potatoes solanum tuberosum l
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Jan Bárta, Veronika Bártová, Zbyněk Zdrahal, Ondrej SedoAbstract:Biochemical characteristics of Patatin proteins purified by ion-exchange and affinity chromatography from tubers of 20 potato cultivars were studied to evaluate their genotype differences with resp...
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chemical composition and nutritional value of protein concentrates isolated from potato solanum tuberosum l fruit juice by precipitation with ethanol or ferric chloride
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Veronika Bártová, Jan BártaAbstract:Effects of protein precipitators, ethanol and ferric chloride, on yield, resolubility, chemical composition and nutritional value of protein concentrates isolated from industrial potato fruit juice (PFJ) were studied. Optimum precipitating concentrations of ethanol and ferric chloride in PFJ were 4 M (23.1% v/v) and 20 mM (2% w/v), resulting in yield of 69% and 86.5% of total protein, respectively. Contents of total glycoalkaloids and potassium in both protein concentrates were significantly lower (P < 0.05) as compared with contents in PFJ dry matter. Both protein concentrates exhibited high nutritional value; values of essential amino acid index (EAAI) were 81.7% and 82.7%, respectively. Fraction of Patatin proteins (39−43 kDa) represented with EAAI value of 86.1% the nutritionally improving protein component. Lipid acyl hydrolase activity of Patatin family was not negatively affected by cooled ethanol precipitation. It can be thus suggested that biological and enzymatic activities of this protein famil...
Jan Bárta - One of the best experts on this subject based on the ideXlab platform.
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Antifungal and antimicrobial proteins and peptides of potato (Solanum tuberosum L.) tubers and their applications
Applied Microbiology and Biotechnology, 2019Co-Authors: Veronika Bártová, Jan Bárta, Markéta JarošováAbstract:Potato proteins are well known for their nutritional, emulsifying, foaming, gel forming or antioxidant properties that all make from them valuable protein source for food industry. Antifungal, antimicrobial and also antiviral properties, described for potato proteins in the review, enrich the possibilities of potato protein usage. Potato proteins were divided into Patatin, protease inhibitors and fraction of other proteins that also included, besides others, proteins involved in potato defence physiology. All these proteins groups provide proteins and peptides with antifungal and/or antimicrobial actions. Patatins, obtained from cultivars with resistance to Phytophthora infestans , were able to inhibit spore germination of this pathogen. Protease inhibitors represent the structurally heterogeneous group with broad range of antifungal and antimicrobial activities. Potato protease inhibitors I and II reduced the growth of Phytophthora infestans , Rhizoctonia solani and Botrytis cinerea or of the fungi of Fusarium genus. Members of Kunitz family (proteins Potide-G, AFP-J, Potamin-1 or PG-2) were able to inhibit serious pathogens such as Staphylococcus aureus , Listeria monocytogenes , Escherichia coli or Candida albicans. Potato snakins, defensins and pseudothionins are discussed for their ability to inhibit serious potato fungi as well as bacterial pathogens. Potato proteins with the ability to inhibit growth of pathogens were used for developing of pathogen-resistant transgenic plants for crop improvement. Incorporation of potato antifungal and antimicrobial proteins in feed and food products or food packages for elimination of hygienically risk pathogens brings new possibility of potato protein usage.
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Patatin the major protein of potato solanum tuberosum l tubers and its occurrence as genotype effect processing versus table potatoes
Czech Journal of Food Sciences, 2018Co-Authors: Jan Bárta, Veronika BártováAbstract:Patatin relative abundance in SDS-extractable protein and Patatin content in dry matter were evaluated in tubers of forty processing and table potato cultivars usually cultivated in the Czech Republic, Germany, and the Netherlands. The Patatin characteristics were evaluated over three experimental years. Patatin relative abundance in the processing cultivars achieved on average a significantly higher value (P < 0.001; Tukey HSD test) than Patatin relative abundance in the table cultivars, resulting in average values of 25.80% and 21.59%, respectively. A high Patatin relative abundance (over 30% in extractable protein) was determined only in the case of two cultivars: Vaneda (average 31.29%) and Tomensa (average 31.24%). Patatin content in tuber dry matter was significantly higher in the processing potato cultivars in all three experimental years (P < 0.001), attaining a mean of 1.28% with the processing cultivars and 1.03% with the table cultivars. The direct effect of the cultivar on Patatin relative abundance in SDS-extractable protein was higher (33.1% for processing potato cultivars and 48.1% for table potato cultivars) than the effect of the growing year (15.6% for processing potato cultivar and 22.8% for table potato cultivars).
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amino acid composition and nutritional value of four cultivated south american potato species
Journal of Food Composition and Analysis, 2015Co-Authors: Veronika Bártová, Jan Bárta, Adela Brabcova, Zbyněk Zdrahal, Vendulka HorackovaAbstract:Protein content, amino acid composition, nutritional quality and Patatin characteristics were determined in tubers of four South American cultivated potato species (Solanum andigenum, Solanum goniocalyx, Solanum phureja, Solanum stenotomum) and a cultivar of the commonly cultivated cultivar Desiree of Solanum tuberosum. Protein content (on dry matter basis) of S. andigenum and S. stenotomum was highest at 7.9% and 8.0%, respectively, and the relative quantities of Patatin for both species were 41.7% and 34.0%, respectively. The nutritional value of Patatin, in terms of essential amino acid index with respect to a reference protein of FAO/WHO (EAAI(adult)) ranged from 93.0% (S. phureja) to 112.5% (S. goniocalyx). In case S. goniocalyx, the Patatin fraction was a nutritionally better protein fraction than a protein concentrate or tuber dry matter, which had EAAI(adult) values of 97.6% and 82.9%, respectively. This suggests that this species may have potential in potato breeding programmes and in human nutrition.
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cultivar variability of Patatin biochemical characteristics table versus processing potatoes solanum tuberosum l
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Jan Bárta, Veronika Bártová, Zbyněk Zdrahal, Ondrej SedoAbstract:Biochemical characteristics of Patatin proteins purified by ion-exchange and affinity chromatography from tubers of 20 potato cultivars were studied to evaluate their genotype differences with resp...
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chemical composition and nutritional value of protein concentrates isolated from potato solanum tuberosum l fruit juice by precipitation with ethanol or ferric chloride
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Veronika Bártová, Jan BártaAbstract:Effects of protein precipitators, ethanol and ferric chloride, on yield, resolubility, chemical composition and nutritional value of protein concentrates isolated from industrial potato fruit juice (PFJ) were studied. Optimum precipitating concentrations of ethanol and ferric chloride in PFJ were 4 M (23.1% v/v) and 20 mM (2% w/v), resulting in yield of 69% and 86.5% of total protein, respectively. Contents of total glycoalkaloids and potassium in both protein concentrates were significantly lower (P < 0.05) as compared with contents in PFJ dry matter. Both protein concentrates exhibited high nutritional value; values of essential amino acid index (EAAI) were 81.7% and 82.7%, respectively. Fraction of Patatin proteins (39−43 kDa) represented with EAAI value of 86.1% the nutritionally improving protein component. Lipid acyl hydrolase activity of Patatin family was not negatively affected by cooled ethanol precipitation. It can be thus suggested that biological and enzymatic activities of this protein famil...
Stacy D Singer - One of the best experts on this subject based on the ideXlab platform.
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castor Patatin like phospholipase a iiiβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic arabidopsis seeds
Plant Molecular Biology, 2019Co-Authors: Guanqun Chen, Elzbieta Mietkiewska, Ziliang Song, Kristian Mark P Caldo, Stacy D Singer, John M Dyer, Mark Smith, Thomas A Mckeon, Randall J WeselakeAbstract:Castor Patatin-like phospholipase A IIIβ facilitates the exclusion of hydroxy fatty acids from phosphatidylcholine in developing transgenic Arabidopsis seeds. Hydroxy fatty acids (HFAs) are industrial useful, but their major natural source castor contains toxic components. Although expressing a castor OLEATE 12-HYDROXYLASE in Arabidopsis thaliana leads to the synthesis of HFAs in seeds, a high proportion of the HFAs are retained in phosphatidylcholine (PC). Thus, the liberation of HFA from PC seems to be critical for obtaining HFA-enriched seed oils. Plant phospholipase A (PLA) catalyzes the hydrolysis of PC to release fatty acyl chains that can be subsequently channeled into triacylglycerol (TAG) synthesis or other metabolic pathways. To further our knowledge regarding the function of PLAs from HFA-producing plant species, two class III Patatin-like PLA cDNAs (pPLAIIIβ or pPLAIIIδ) from castor or Physaria fendleri were overexpressed in a transgenic line of A. thaliana producing C18-HFA, respectively. Only the overexpression of RcpPLAIIIβ resulted in a significant reduction in seed HFA content with concomitant changes in fatty acid composition. Reductions in HFA content occurred in both PC and TAG indicating that HFAs released from PC were not incorporated into TAG. These results suggest that RcpPLAIIIβ may catalyze the removal of HFAs from PC in the developing seeds synthesizing these unusual fatty acids.
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castor Patatin like phospholipase a iiiβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic arabidopsis seeds
Plant Molecular Biology, 2019Co-Authors: Yingyu Lin, Guanqun Chen, Elzbieta Mietkiewska, Ziliang Song, Kristian Mark P Caldo, Stacy D Singer, John M Dyer, Mark Smith, Thomas A MckeonAbstract:Castor Patatin-like phospholipase A IIIβ facilitates the exclusion of hydroxy fatty acids from phosphatidylcholine in developing transgenic Arabidopsis seeds. Hydroxy fatty acids (HFAs) are industrial useful, but their major natural source castor contains toxic components. Although expressing a castor OLEATE 12-HYDROXYLASE in Arabidopsis thaliana leads to the synthesis of HFAs in seeds, a high proportion of the HFAs are retained in phosphatidylcholine (PC). Thus, the liberation of HFA from PC seems to be critical for obtaining HFA-enriched seed oils. Plant phospholipase A (PLA) catalyzes the hydrolysis of PC to release fatty acyl chains that can be subsequently channeled into triacylglycerol (TAG) synthesis or other metabolic pathways. To further our knowledge regarding the function of PLAs from HFA-producing plant species, two class III Patatin-like PLA cDNAs (pPLAIIIβ or pPLAIIIδ) from castor or Physaria fendleri were overexpressed in a transgenic line of A. thaliana producing C18-HFA, respectively. Only the overexpression of RcpPLAIIIβ resulted in a significant reduction in seed HFA content with concomitant changes in fatty acid composition. Reductions in HFA content occurred in both PC and TAG indicating that HFAs released from PC were not incorporated into TAG. These results suggest that RcpPLAIIIβ may catalyze the removal of HFAs from PC in the developing seeds synthesizing these unusual fatty acids.
Hoong Yeet Yeang - One of the best experts on this subject based on the ideXlab platform.
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isolation and characterization of the early nodule specific protein homologue hev b 13 an allergenic lipolytic esterase from hevea brasiliensis latex
Journal of Biological Chemistry, 2004Co-Authors: S A M Arif, Robert G Hamilton, Faridah Yusof, Nyu Ping Chew, Y H Loke, Subodh B Nimkar, Jaap J Beintema, Hoong Yeet YeangAbstract:Recurring reports of a highly allergenic 42-46-kDa protein in Hevea brasiliensis latex appeared to have been resolved with the discovery of a 43-kDa allergenic latex protein that was a homologue to Patatin. However, the low to moderate prevalence of sensitization to the protein, designated Hev b 7, among latex-allergic patients could not adequately explain the frequent observations of the 42-46-kDa allergen. This led to the hypothesis that another, more allergenic protein of a similar molecular mass existed in Hevea latex. We report the isolation and purification of a 42.98-kDa latex glycoprotein showing homology to the early nodule-specific protein (ENSP) of the legumes Medicago sativa, Medicago truncatula, and Glycine max. The protein is allergenic, being recognized by immunoglobulin E (IgE) in sera from latex-allergic patients. The IgE epitope resides on the carbohydrate moiety of the protein, and the presence of a similar carbohydrate component on potato tuber Patatin enables the latter to inhibit IgE binding to the ENSP homologue. The cDNA encoding the ENSP homologue was isolated by reverse transcription-PCR and cloned. The protein predicted from the cDNA sequence has 391 amino acids, the first 26 of which constitute a putative signal peptide. The deduced molecular mass of the mature protein is 40.40 kDa, while its isoelectric point is estimated at 5.0. The discrepancy between the predicted and observed molecular mass might be due to glycosylation, for which three N-sites on the protein are predicted. The purified protein showed lipase and esterase activities and may be involved in plant defense.
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isolation and characterization of the early nodule specific protein homologue hev b 13 an allergenic lipolytic esterase from hevea brasiliensis latex
The Journal of Allergy and Clinical Immunology, 2004Co-Authors: S A M Arif, Robert G Hamilton, Faridah Yusof, Nyu Ping Chew, Y H Loke, Subodh B Nimkar, Jaap J Beintema, Hoong Yeet YeangAbstract:Abstract Rationale Identification of Hev b 7, a 43 kDa latex allergen homologue of Patatin, did not adequately explain the frequent observations of IgE antibody reactivity to a 42–46 kDa protein in serum from latex-allergic patients. We thus hypothesized the existence of another allergenic Hevea protein of a similar molecular weight. We report on the isolation and purification of a 42.98 kDa latex glycoprotein showing homology to Early Nodule Specific Protein in legumes. Methods Hev b 13 was isolated from Hevea brasiliensis latex B-serum by gel filtration and ionic exchange chromatography. Binding of human IgE to Hev b 13 and inhibition of IgE binding by Patatin were studied by Western immuno-blots analysis. The cDNA encoding Hev b 13 was isolated by RT-PCR and cloned. Results The protein predicted from the Hev b 13 cDNA sequence has 391 amino acids. The mature protein possessed a deduced molecular weight of 40.40 kDa and pI of 5.0. Prevalence of IgE antibody reactivity to native Hev b 13 was 78% (51/65) in latex-sensitized healthcare workers and 77% (10/13) in latex-sensitized spina bifida children. IgE-binding to Hev b 13 was inhibited by the potato tuber Patatin but lost when the carbohydrate moiety of Patatin was removed. This suggested that the IgE-specific epitope might reside on the glycan. Conclusions The lipolytic esterase, Hev b 13, is a major allergen of Hevea brasiliensis latex. The principal IgE-specific epitope of the glycoprotein appears to be located on its carbohydrate moiety.
Thomas A Mckeon - One of the best experts on this subject based on the ideXlab platform.
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castor Patatin like phospholipase a iiiβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic arabidopsis seeds
Plant Molecular Biology, 2019Co-Authors: Guanqun Chen, Elzbieta Mietkiewska, Ziliang Song, Kristian Mark P Caldo, Stacy D Singer, John M Dyer, Mark Smith, Thomas A Mckeon, Randall J WeselakeAbstract:Castor Patatin-like phospholipase A IIIβ facilitates the exclusion of hydroxy fatty acids from phosphatidylcholine in developing transgenic Arabidopsis seeds. Hydroxy fatty acids (HFAs) are industrial useful, but their major natural source castor contains toxic components. Although expressing a castor OLEATE 12-HYDROXYLASE in Arabidopsis thaliana leads to the synthesis of HFAs in seeds, a high proportion of the HFAs are retained in phosphatidylcholine (PC). Thus, the liberation of HFA from PC seems to be critical for obtaining HFA-enriched seed oils. Plant phospholipase A (PLA) catalyzes the hydrolysis of PC to release fatty acyl chains that can be subsequently channeled into triacylglycerol (TAG) synthesis or other metabolic pathways. To further our knowledge regarding the function of PLAs from HFA-producing plant species, two class III Patatin-like PLA cDNAs (pPLAIIIβ or pPLAIIIδ) from castor or Physaria fendleri were overexpressed in a transgenic line of A. thaliana producing C18-HFA, respectively. Only the overexpression of RcpPLAIIIβ resulted in a significant reduction in seed HFA content with concomitant changes in fatty acid composition. Reductions in HFA content occurred in both PC and TAG indicating that HFAs released from PC were not incorporated into TAG. These results suggest that RcpPLAIIIβ may catalyze the removal of HFAs from PC in the developing seeds synthesizing these unusual fatty acids.
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castor Patatin like phospholipase a iiiβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic arabidopsis seeds
Plant Molecular Biology, 2019Co-Authors: Yingyu Lin, Guanqun Chen, Elzbieta Mietkiewska, Ziliang Song, Kristian Mark P Caldo, Stacy D Singer, John M Dyer, Mark Smith, Thomas A MckeonAbstract:Castor Patatin-like phospholipase A IIIβ facilitates the exclusion of hydroxy fatty acids from phosphatidylcholine in developing transgenic Arabidopsis seeds. Hydroxy fatty acids (HFAs) are industrial useful, but their major natural source castor contains toxic components. Although expressing a castor OLEATE 12-HYDROXYLASE in Arabidopsis thaliana leads to the synthesis of HFAs in seeds, a high proportion of the HFAs are retained in phosphatidylcholine (PC). Thus, the liberation of HFA from PC seems to be critical for obtaining HFA-enriched seed oils. Plant phospholipase A (PLA) catalyzes the hydrolysis of PC to release fatty acyl chains that can be subsequently channeled into triacylglycerol (TAG) synthesis or other metabolic pathways. To further our knowledge regarding the function of PLAs from HFA-producing plant species, two class III Patatin-like PLA cDNAs (pPLAIIIβ or pPLAIIIδ) from castor or Physaria fendleri were overexpressed in a transgenic line of A. thaliana producing C18-HFA, respectively. Only the overexpression of RcpPLAIIIβ resulted in a significant reduction in seed HFA content with concomitant changes in fatty acid composition. Reductions in HFA content occurred in both PC and TAG indicating that HFAs released from PC were not incorporated into TAG. These results suggest that RcpPLAIIIβ may catalyze the removal of HFAs from PC in the developing seeds synthesizing these unusual fatty acids.