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Agung Sosiawan - One of the best experts on this subject based on the ideXlab platform.
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kinship analysis on Paternity Test through str codis locus csf1po thoi tpox vwa from maduranese siblings in surabaya
2021Co-Authors: Ahmad Yudianto, Fery Setiawan, Agung Sosiawan, Reni SuminoAbstract:Introduction: Paternity Test is a DNA examination to determine whether a man is a biological father of a child through DNA pattern comparison between the child and the suspected father to verify DNA inheritance proof. Methods: This study was conducted through analysis of kinship analysis application on Paternity Test through STR CODIS examination in siblings using peripheral bloods samples of forty samples Madurese 3 generations. The examined locuses were CSF1PO, TH01, TPOX, vWA. Results: DNA samples isolates found that the mean level of DNA was 595.35 ± 4.15ng/µl, whereas the range of purity was 1.17 – 1.29. From this study, alleles from maduranese family population in Surabaya was variable, the highest percentage was CSF1PO locus allele 9 [33.75%], THO1 allele 8 [26.25%], TPOX allele 9 [32.5%] and vWA allele 17 [21.25%]. This study also revealed shared alleles on one sibling allele, the highest was 2 shared alleles on CSF1PO [70%], THO1 [100%], TPOX [70%] and vWA locus [90%]. Conclusion: THOI locus had 100% 2 shared alleles so that it was recommend using STR locus to identify kinship on Madurese in Surabaya.
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Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal Paternity diagnostic Tests
Springer Berlin Heidelberg, 2018Co-Authors: Agung Sosiawan, Indah Nuraini, Alexander Patera Nugraha, Didik Raharjo, Nadia Kartika, Muhammad DimasAbstract:lntroduction: A prenatal Paternity lest is one widely-used method of determining the Paternity of an unborn child. Such Tests using chorionic villus or amniocentesis may increase the risk of harm to both mother and foetus. ln the presenr day, a [renatal Paternity Test using circulating cell-free fetal Deoxyribonucleic Acid (DNA) is one alternative method due to it being non-invasive and safe for both mother and foetus. Aim: The aim of this study is to detect Short Tandem Repeat (STR) at 5 Loci (vWA, TH01, Dl3S317, Dl8551, and D21 51 1) and amelogenin genes in circulating cell-free fetal DNA in Paternity Tests. Materials and methods: Forty-one samples of maternal blood were obtained from pregnant woman. circulating free fetal DNA was subsequently egracted. A Paternity Test was conducted using an SfR Test at loci vWA TH01. Dl35312 Dl8S5l, D2'tS11 in circulating free fetal DNA An analysis of the Paternity Test between loci > 200 bp and < 200 bp was also conducted to establish the sensitivity of the Test Results: There was a significant difference bewveen maternal blood DNA and circulating cell-free feUl DNA (o = 0.000 D13S3i7; p=O.OOO D21511; p=0.000 D18S51;p=0.000 vWA; p=0.000 THO1;and p=0.000 amelogenin genes). The locus 200 bp. Conclusion: Circulating free fetal DNA can be used as an alternative sample for prenatal Paternity Tests because of its similarity with maternal DNA. Keywords: Early detection, Circulating free fetal DNA, Non-invasive technique, Prenatal Paternity tes
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Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal Paternity diagnostic Tests
SpringerOpen, 2018Co-Authors: Agung Sosiawan, Dadik Raharjo, Indah Nuraini, Nadia Kartikasari, Alexander Patera Nugraha, Muhammad Dimas Aditya AriAbstract:Abstract Introduction A prenatal Paternity Test is one widely-used method of determining the Paternity of an unborn child. Such Tests using chorionic villus or amniocentesis may increase the risk of harm to both mother and foetus. In the present day, a prenatal Paternity Test using circulating cell-free fetal Deoxyribonucleic Acid (DNA) is one alternative method due to it being non-invasive and safe for both mother and foetus. Aim The aim of this study is to detect Short Tandem Repeat (STR) at 5 Loci (vWA, TH01, D13S317, D18S51, and D21S11) and amelogenin genes in circulating cell-free fetal DNA in Paternity Tests. Materials and methods Forty-one samples of maternal blood were obtained from pregnant woman. Circulating free fetal DNA was subsequently extracted. A Paternity Test was conducted using an STR Test at loci vWA, TH01, D13S317, D18S51, D21S11 in circulating free fetal DNA. An analysis of the Paternity Test between loci > 200 bp and
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Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal Paternity diagnostic Tests
'Springer Science and Business Media LLC', 2018Co-Authors: Agung Sosiawan, Dadik Raharjo, Indah Nuraini, Nadia Kartikasari, Alexander Patera Nugraha, Muhammad Dimas Aditya AriAbstract:Abstract Introduction A prenatal Paternity Test is one widely-used method of determining the Paternity of an unborn child. Such Tests using chorionic villus or amniocentesis may increase the risk of harm to both mother and foetus. In the present day, a prenatal Paternity Test using circulating cell-free fetal Deoxyribonucleic Acid (DNA) is one alternative method due to it being non-invasive and safe for both mother and foetus. Aim The aim of this study is to detect Short Tandem Repeat (STR) at 5 Loci (vWA, TH01, D13S317, D18S51, and D21S11) and amelogenin genes in circulating cell-free fetal DNA in Paternity Tests. Materials and methods Forty-one samples of maternal blood were obtained from pregnant woman. Circulating free fetal DNA was subsequently extracted. A Paternity Test was conducted using an STR Test at loci vWA, TH01, D13S317, D18S51, D21S11 in circulating free fetal DNA. An analysis of the Paternity Test between loci > 200 bp and 200 bp. Conclusion Circulating free fetal DNA can be used as an alternative sample for prenatal Paternity Tests because of its similarity with maternal DNA
Fang Chen - One of the best experts on this subject based on the ideXlab platform.
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noninvasive prenatal Paternity Testing nipat through maternal plasma dna sequencing a pilot study
PLOS ONE, 2016Co-Authors: Haojun Jiang, Xuchao Li, Huijuan Ge, Haofang Mu, Xiaoli Feng, Zhou Du, Yongqiang Deng, Fang ChenAbstract:Short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs) have been already used to perform noninvasive prenatal Paternity Testing from maternal plasma DNA. The frequently used technologies were PCR followed by capillary electrophoresis and SNP typing array, respectively. Here, we developed a noninvasive prenatal Paternity Testing (NIPAT) based on SNP typing with maternal plasma DNA sequencing. We evaluated the influence factors (minor allele frequency (MAF), the number of total SNP, fetal fraction and effective sequencing depth) and designed three different selective SNP panels in order to verify the performance in clinical cases. Combining targeted deep sequencing of selective SNP and informative bioinformatics pipeline, we calculated the combined Paternity index (CPI) of 17 cases to determine Paternity. Sequencing-based NIPAT results fully agreed with invasive prenatal Paternity Test using STR multiplex system. Our study here proved that the maternal plasma DNA sequencing-based technology is feasible and accurate in determining Paternity, which may provide an alternative in forensic application in the future.
Matthew Rabinowitz - One of the best experts on this subject based on the ideXlab platform.
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informatics based highly accurate noninvasive prenatal Paternity Testing
Genetics in Medicine, 2013Co-Authors: Allison Ryan, Johan Baner, Zachary Demko, Matthew Hill, Styrmir Sigurjonsson, Michael L Baird, Matthew RabinowitzAbstract:The aim of the study was to evaluate the diagnostic accuracy of an informatics-based, noninvasive, prenatal Paternity Test using array-based single-nucleotide polymorphism measurements of cell-free DNA isolated from maternal plasma. Blood samples were taken from 21 adult pregnant women (with gestational ages between 6 and 21 weeks), and a genetic sample was taken from the corresponding biological fathers. Paternity was confirmed by genetic Testing of the infant, products of conception, control of fertilization, and/or preimplantation genetic diagnosis during in vitro fertilization. Parental DNA samples and maternal plasma cell-free DNA were amplified and analyzed using a HumanCytoSNP-12 array. An informatics-based method measured single-nucleotide polymorphism data, confirming or rejecting Paternity. Each plasma sample with a sufficient fetal cell-free DNA fraction was independently Tested against the confirmed father and 1,820 random, unrelated males. One of the 21 samples had insufficient fetal cell-free DNA. The Test correctly confirmed Paternity for the remaining 20 samples (100%) when Tested against the biological father, with P values of <10−4. For the 36,400 Tests using an unrelated male as the alleged father, 99.95% (36,382) correctly excluded Paternity and 0.05% (18) were indeterminate. There were no miscalls. A noninvasive Paternity Test using informatics-based analysis of single-nucleotide polymorphism array measurements accurately determined Paternity early in pregnancy. Genet Med 2013:15(6):473–477
Haofang Mu - One of the best experts on this subject based on the ideXlab platform.
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noninvasive prenatal Paternity Testing nipat through maternal plasma dna sequencing a pilot study
PLOS ONE, 2016Co-Authors: Haojun Jiang, Xuchao Li, Huijuan Ge, Haofang Mu, Xiaoli Feng, Zhou Du, Yongqiang Deng, Fang ChenAbstract:Short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs) have been already used to perform noninvasive prenatal Paternity Testing from maternal plasma DNA. The frequently used technologies were PCR followed by capillary electrophoresis and SNP typing array, respectively. Here, we developed a noninvasive prenatal Paternity Testing (NIPAT) based on SNP typing with maternal plasma DNA sequencing. We evaluated the influence factors (minor allele frequency (MAF), the number of total SNP, fetal fraction and effective sequencing depth) and designed three different selective SNP panels in order to verify the performance in clinical cases. Combining targeted deep sequencing of selective SNP and informative bioinformatics pipeline, we calculated the combined Paternity index (CPI) of 17 cases to determine Paternity. Sequencing-based NIPAT results fully agreed with invasive prenatal Paternity Test using STR multiplex system. Our study here proved that the maternal plasma DNA sequencing-based technology is feasible and accurate in determining Paternity, which may provide an alternative in forensic application in the future.
Xiaoli Feng - One of the best experts on this subject based on the ideXlab platform.
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noninvasive prenatal Paternity Testing nipat through maternal plasma dna sequencing a pilot study
PLOS ONE, 2016Co-Authors: Haojun Jiang, Xuchao Li, Huijuan Ge, Haofang Mu, Xiaoli Feng, Zhou Du, Yongqiang Deng, Fang ChenAbstract:Short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs) have been already used to perform noninvasive prenatal Paternity Testing from maternal plasma DNA. The frequently used technologies were PCR followed by capillary electrophoresis and SNP typing array, respectively. Here, we developed a noninvasive prenatal Paternity Testing (NIPAT) based on SNP typing with maternal plasma DNA sequencing. We evaluated the influence factors (minor allele frequency (MAF), the number of total SNP, fetal fraction and effective sequencing depth) and designed three different selective SNP panels in order to verify the performance in clinical cases. Combining targeted deep sequencing of selective SNP and informative bioinformatics pipeline, we calculated the combined Paternity index (CPI) of 17 cases to determine Paternity. Sequencing-based NIPAT results fully agreed with invasive prenatal Paternity Test using STR multiplex system. Our study here proved that the maternal plasma DNA sequencing-based technology is feasible and accurate in determining Paternity, which may provide an alternative in forensic application in the future.