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David A Mitchell - One of the best experts on this subject based on the ideXlab platform.
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production of Pectinases by solid state fermentation of a mixture of citrus waste and sugarcane bagasse in a pilot scale packed bed bioreactor
Biochemical Engineering Journal, 2016Co-Authors: Alessandra Biz, Anelize Terezinha Jung Finkler, Luana Oliveira Pitol, Bruna Schweitzer Medina, Nadia Krieger, David A MitchellAbstract:Abstract Pectinases can be used in citrus waste biorefineries to hydrolyze the pectin in citrus pulp to produce d -galacturonic acid, a potential platform chemical. Solid-state fermentation has the potential to produce low-cost Pectinases for such biorefineries, but it is difficult to control the process at large scales. In the current work, Aspergillus oryzae was cultivated in a pilot-scale packed-bed bioreactor, on 15 kg of a substrate containing 51.6% citrus pulp and 48.4% sugarcane bagasse (w/w, dry basis). The sugarcane bagasse gave a high bed porosity and ensured a stable bed structure, avoiding problems of bed shrinkage and the formation of compact agglomerates within the bed. As a result, bed temperatures were controlled to within 1 °C of the inlet air temperature and pectinase yields of 33–41 U g −1 were obtained across the bed. When the fermented solids were dried and added directly to a pectin solution, they gave a profile for the release of d -galacturonic acid similar to that obtained with a commercial pectinase. These results show the potential for using solid-state fermentation to produce Pectinases in a citrus waste biorefinery, with subsequent direct addition of the fermented solids to produce d -galacturonic acid from the pectin contained in the citrus pulp.
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production of Pectinases by solid state fermentation in a pilot scale packed bed bioreactor
Chemical Engineering Journal, 2016Co-Authors: Luana Oliveira Pitol, Alessandra Biz, Nadia Krieger, Edgar Mallmann, David A MitchellAbstract:Abstract Solid-state fermentation can be used to produce Pectinases using agro-industrial byproducts. However, heat and mass transfer limitations make it difficult to control the temperature within the bioreactor, especially at large scale, so reliable scale-up strategies are essential. In the current work, we scaled up the production of Pectinases in packed-bed bioreactors, from 12 g to 30 kg of dry substrate, the biggest scale yet reported for pectinase production. When compaction occurred, bed temperatures up to 47 °C were recorded and the pectinase activity in different regions of the bed at 26 h varied from 11 to 28 × 10 3 U kg −1 . When compaction was avoided, the maximum bed temperature was 32 °C and the pectinase activity at 26 h varied from 17 to 20 × 10 3 U kg −1 . The best result was obtained with a 40-cm high bed containing 27 kg of wheat bran and 3 kg of sugarcane bagasse, with switching of the temperature of the saturated inlet air between 24 °C and 32 °C. Under these conditions, the maximum productivity was 1840 U kg −1 h −1 at 10 h. We propose that the process can be scaled up to production scale by maintaining the same bed height and operational strategy, while increasing the width of the bed to several meters. If the superficial velocity of the air is maintained constant at 0.1 m s −1 , then the performance at scales involving several tonnes of solid substrate should be similar to that obtained in pilot-scale bioreactor in the current study.
Maedeh Mohammadi - One of the best experts on this subject based on the ideXlab platform.
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bioconversion of agroindustrial wastes to Pectinases enzyme via solid state fermentation in trays and rotating drum bioreactors
Biocatalysis and agricultural biotechnology, 2019Co-Authors: M Mahmoodi, Ghasem D Najafpour, Maedeh MohammadiAbstract:Abstract In this work, production of polygalacturonases through solid-state fermentation of orange pomace by A. niger was studied. Bench-scale pectinase productions in tray and rotating-drum bioreactors were compared. Tray bioreactor was more suitable for Pectinases production; despite the fact that moisture control was much better in rotating-drum bioreactor, while agitation and watering was carried out in the fermentation. Exo- and endo-Pectinases production yields in tray bioreactor were 45 and 37% higher than rotating-drum bioreactor, respectively. Moreover, to solve dryness in tray bioreactor, sugarcane bagasse was studied as a water preservative material. Comparing the obtained water sorption isotherms for bagasse and orange pomace indicated that addition of bagasse to orange pomace for preservation of water in the fermentation was a great help. This issue was experimented in tray bioreactor and it was proved that bagasse enhanced Pectinases production from orange pomace. The percentage of bagasse for preservation of moisture in Pectinases production was optimized. The obtained results demonstrated that addition of bagasse (40 g bagasse. g−1dry solid) to orange pomace led to 17 and 23% enhancement in exo- and endo-pectinase activities, respectively. Finally, enzyme kinetics for exo- and endo-Pectinases were investigated.
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production of Pectinases for quality apple juice through fermentation of orange pomace
Journal of Food Science and Technology-mysore, 2017Co-Authors: M Mahmoodi, Ghasem D Najafpour, Maedeh MohammadiAbstract:Production of Pectinases by Aspergillus niger was successfully carried out through solid state fermentation. Orange pomace was used as substrate to produce Pectinases using a wild type of A. niger isolated from a rotten orange texture. Some of the important parameters affecting exo- and endo-Pectinases activities such as temperature, moisture, C/N ratio were optimized. The results indicated that the produced Pectinases exhibited maximum activity in temperature range of 45–55 °C and the maximum enzyme productivity occurred at 70% moisture content and C/N ratio of 10. The enzyme kinetic was studied using Michaelis–Menten and Logistic model and the equation were fitted to experimental data for both exo- and endo-Pectinases activities. In evaluation of kinetic model, it was found that Monod model presented perfectly fitted with experimental data. Monod kinetic parameters \( (\mu_{max} \;{\text{and}}\;K_{S} ) \) for exo-pectinase activities were \( 771.7\,\upmu{\text{M}}\,{ \hbox{min} }^{ - 1} \;{\text{and}}\;31.91 \) mM, respectively. The Monod kinetic parameters \( ( \mu_{max} \;{\text{and}}\;K_{S} ) \) for endo-pectinase activity were \( 48.19\,{\text{mP}}\;{ \hbox{min} }^{ - 1} \) and \( 478.3\,{\text{mM}}, \) respectively. Finally, the performances of the produced Pectinases were evaluated on natural apple juice. It was confirmed that concentration of soluble sugar, clarity and viscosity of the juice and the yield of extracted juice were significantly improved by the enzymatic hydrolysis activity of Pectinases.
Alessandra Biz - One of the best experts on this subject based on the ideXlab platform.
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production of Pectinases by solid state fermentation of a mixture of citrus waste and sugarcane bagasse in a pilot scale packed bed bioreactor
Biochemical Engineering Journal, 2016Co-Authors: Alessandra Biz, Anelize Terezinha Jung Finkler, Luana Oliveira Pitol, Bruna Schweitzer Medina, Nadia Krieger, David A MitchellAbstract:Abstract Pectinases can be used in citrus waste biorefineries to hydrolyze the pectin in citrus pulp to produce d -galacturonic acid, a potential platform chemical. Solid-state fermentation has the potential to produce low-cost Pectinases for such biorefineries, but it is difficult to control the process at large scales. In the current work, Aspergillus oryzae was cultivated in a pilot-scale packed-bed bioreactor, on 15 kg of a substrate containing 51.6% citrus pulp and 48.4% sugarcane bagasse (w/w, dry basis). The sugarcane bagasse gave a high bed porosity and ensured a stable bed structure, avoiding problems of bed shrinkage and the formation of compact agglomerates within the bed. As a result, bed temperatures were controlled to within 1 °C of the inlet air temperature and pectinase yields of 33–41 U g −1 were obtained across the bed. When the fermented solids were dried and added directly to a pectin solution, they gave a profile for the release of d -galacturonic acid similar to that obtained with a commercial pectinase. These results show the potential for using solid-state fermentation to produce Pectinases in a citrus waste biorefinery, with subsequent direct addition of the fermented solids to produce d -galacturonic acid from the pectin contained in the citrus pulp.
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production of Pectinases by solid state fermentation in a pilot scale packed bed bioreactor
Chemical Engineering Journal, 2016Co-Authors: Luana Oliveira Pitol, Alessandra Biz, Nadia Krieger, Edgar Mallmann, David A MitchellAbstract:Abstract Solid-state fermentation can be used to produce Pectinases using agro-industrial byproducts. However, heat and mass transfer limitations make it difficult to control the temperature within the bioreactor, especially at large scale, so reliable scale-up strategies are essential. In the current work, we scaled up the production of Pectinases in packed-bed bioreactors, from 12 g to 30 kg of dry substrate, the biggest scale yet reported for pectinase production. When compaction occurred, bed temperatures up to 47 °C were recorded and the pectinase activity in different regions of the bed at 26 h varied from 11 to 28 × 10 3 U kg −1 . When compaction was avoided, the maximum bed temperature was 32 °C and the pectinase activity at 26 h varied from 17 to 20 × 10 3 U kg −1 . The best result was obtained with a 40-cm high bed containing 27 kg of wheat bran and 3 kg of sugarcane bagasse, with switching of the temperature of the saturated inlet air between 24 °C and 32 °C. Under these conditions, the maximum productivity was 1840 U kg −1 h −1 at 10 h. We propose that the process can be scaled up to production scale by maintaining the same bed height and operational strategy, while increasing the width of the bed to several meters. If the superficial velocity of the air is maintained constant at 0.1 m s −1 , then the performance at scales involving several tonnes of solid substrate should be similar to that obtained in pilot-scale bioreactor in the current study.
Luana Oliveira Pitol - One of the best experts on this subject based on the ideXlab platform.
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production of Pectinases by solid state fermentation of a mixture of citrus waste and sugarcane bagasse in a pilot scale packed bed bioreactor
Biochemical Engineering Journal, 2016Co-Authors: Alessandra Biz, Anelize Terezinha Jung Finkler, Luana Oliveira Pitol, Bruna Schweitzer Medina, Nadia Krieger, David A MitchellAbstract:Abstract Pectinases can be used in citrus waste biorefineries to hydrolyze the pectin in citrus pulp to produce d -galacturonic acid, a potential platform chemical. Solid-state fermentation has the potential to produce low-cost Pectinases for such biorefineries, but it is difficult to control the process at large scales. In the current work, Aspergillus oryzae was cultivated in a pilot-scale packed-bed bioreactor, on 15 kg of a substrate containing 51.6% citrus pulp and 48.4% sugarcane bagasse (w/w, dry basis). The sugarcane bagasse gave a high bed porosity and ensured a stable bed structure, avoiding problems of bed shrinkage and the formation of compact agglomerates within the bed. As a result, bed temperatures were controlled to within 1 °C of the inlet air temperature and pectinase yields of 33–41 U g −1 were obtained across the bed. When the fermented solids were dried and added directly to a pectin solution, they gave a profile for the release of d -galacturonic acid similar to that obtained with a commercial pectinase. These results show the potential for using solid-state fermentation to produce Pectinases in a citrus waste biorefinery, with subsequent direct addition of the fermented solids to produce d -galacturonic acid from the pectin contained in the citrus pulp.
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production of Pectinases by solid state fermentation in a pilot scale packed bed bioreactor
Chemical Engineering Journal, 2016Co-Authors: Luana Oliveira Pitol, Alessandra Biz, Nadia Krieger, Edgar Mallmann, David A MitchellAbstract:Abstract Solid-state fermentation can be used to produce Pectinases using agro-industrial byproducts. However, heat and mass transfer limitations make it difficult to control the temperature within the bioreactor, especially at large scale, so reliable scale-up strategies are essential. In the current work, we scaled up the production of Pectinases in packed-bed bioreactors, from 12 g to 30 kg of dry substrate, the biggest scale yet reported for pectinase production. When compaction occurred, bed temperatures up to 47 °C were recorded and the pectinase activity in different regions of the bed at 26 h varied from 11 to 28 × 10 3 U kg −1 . When compaction was avoided, the maximum bed temperature was 32 °C and the pectinase activity at 26 h varied from 17 to 20 × 10 3 U kg −1 . The best result was obtained with a 40-cm high bed containing 27 kg of wheat bran and 3 kg of sugarcane bagasse, with switching of the temperature of the saturated inlet air between 24 °C and 32 °C. Under these conditions, the maximum productivity was 1840 U kg −1 h −1 at 10 h. We propose that the process can be scaled up to production scale by maintaining the same bed height and operational strategy, while increasing the width of the bed to several meters. If the superficial velocity of the air is maintained constant at 0.1 m s −1 , then the performance at scales involving several tonnes of solid substrate should be similar to that obtained in pilot-scale bioreactor in the current study.
Nadia Krieger - One of the best experts on this subject based on the ideXlab platform.
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production of Pectinases by solid state fermentation of a mixture of citrus waste and sugarcane bagasse in a pilot scale packed bed bioreactor
Biochemical Engineering Journal, 2016Co-Authors: Alessandra Biz, Anelize Terezinha Jung Finkler, Luana Oliveira Pitol, Bruna Schweitzer Medina, Nadia Krieger, David A MitchellAbstract:Abstract Pectinases can be used in citrus waste biorefineries to hydrolyze the pectin in citrus pulp to produce d -galacturonic acid, a potential platform chemical. Solid-state fermentation has the potential to produce low-cost Pectinases for such biorefineries, but it is difficult to control the process at large scales. In the current work, Aspergillus oryzae was cultivated in a pilot-scale packed-bed bioreactor, on 15 kg of a substrate containing 51.6% citrus pulp and 48.4% sugarcane bagasse (w/w, dry basis). The sugarcane bagasse gave a high bed porosity and ensured a stable bed structure, avoiding problems of bed shrinkage and the formation of compact agglomerates within the bed. As a result, bed temperatures were controlled to within 1 °C of the inlet air temperature and pectinase yields of 33–41 U g −1 were obtained across the bed. When the fermented solids were dried and added directly to a pectin solution, they gave a profile for the release of d -galacturonic acid similar to that obtained with a commercial pectinase. These results show the potential for using solid-state fermentation to produce Pectinases in a citrus waste biorefinery, with subsequent direct addition of the fermented solids to produce d -galacturonic acid from the pectin contained in the citrus pulp.
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production of Pectinases by solid state fermentation in a pilot scale packed bed bioreactor
Chemical Engineering Journal, 2016Co-Authors: Luana Oliveira Pitol, Alessandra Biz, Nadia Krieger, Edgar Mallmann, David A MitchellAbstract:Abstract Solid-state fermentation can be used to produce Pectinases using agro-industrial byproducts. However, heat and mass transfer limitations make it difficult to control the temperature within the bioreactor, especially at large scale, so reliable scale-up strategies are essential. In the current work, we scaled up the production of Pectinases in packed-bed bioreactors, from 12 g to 30 kg of dry substrate, the biggest scale yet reported for pectinase production. When compaction occurred, bed temperatures up to 47 °C were recorded and the pectinase activity in different regions of the bed at 26 h varied from 11 to 28 × 10 3 U kg −1 . When compaction was avoided, the maximum bed temperature was 32 °C and the pectinase activity at 26 h varied from 17 to 20 × 10 3 U kg −1 . The best result was obtained with a 40-cm high bed containing 27 kg of wheat bran and 3 kg of sugarcane bagasse, with switching of the temperature of the saturated inlet air between 24 °C and 32 °C. Under these conditions, the maximum productivity was 1840 U kg −1 h −1 at 10 h. We propose that the process can be scaled up to production scale by maintaining the same bed height and operational strategy, while increasing the width of the bed to several meters. If the superficial velocity of the air is maintained constant at 0.1 m s −1 , then the performance at scales involving several tonnes of solid substrate should be similar to that obtained in pilot-scale bioreactor in the current study.
