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Rupert L. L. Kellner - One of the best experts on this subject based on the ideXlab platform.
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stadium specific transmission of endosymbionts needed for Pederin biosynthesis in three species of paederus rove beetles
Entomologia Experimentalis Et Applicata, 2003Co-Authors: Rupert L. L. KellnerAbstract:Females of the rove beetle genus Paederus (Coleoptera: Staphylinidae) accumulate large amounts of the defensive compound Pederin produced by an endosymbiotic bacterium. Pederin is transferred in the eggs, while the endosymbionts are transmitted via the egg shell. In all three species analyzed (P. melanurus, P. riparius, and P. sabaeus), descendants of aposymbiotic matrilines (lacking both endosymbionts and biosynthetic capabilities) acquire biosynthetic capability by the ingestion of eggs with endosymbionts during larval development. Successful colonization by endosymbionts depends on the number of eggs consumed and the age of the feeding larvae. During the adult stage, however, the females cannot acquire biosynthetic capabilities. Adult females are able to sequester Pederin from eggs eaten, and they transfer the substance into their own eggs. Lack of intrinsic biosynthesis after ingestion of endosymbionts indicates that these are not biosynthetically active within the gut and have to reach an unknown internal location before completion of metamorphosis. These results are discussed with regard to the benefit of intraspecific cannibalism.
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Interspecific transmission of Paederus endosymbionts: relationship to the genetic divergence among the bacteria associated with Pederin biosynthesis
Chemoecology, 2002Co-Authors: Rupert L. L. KellnerAbstract:Endosymbiotic bacteria implicated in Pederin production of Paederus (+)-females (Coleoptera: Staphylinidae) can be transmitted horizontally within and less frequently among the three species analyzed (P. melanurus, P. riparius, P. sabaeus). The 16S rDNA isolated from (+)-females reveals closely related bacterial sequences in the three species as well as in Paederus fuscipes and Paederidus ruficollis. This confirms the association of the undescribed endosymbiont and Pederin biosynthesis in 5 of the 13 species that have been shown to contain the substance. In spite of the high sequence identities (> 99.5%), which suggest one species of endosymbiont, some of the heterospecific hosts were incompatible. This indicates adaptation and specific preferences of the endosymbiont for their natural host.
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Molecular identification of an endosymbiotic bacterium associated with Pederin biosynthesis in Paederus sabaeus (Coleoptera: Staphylinidae).
Insect biochemistry and molecular biology, 2002Co-Authors: Rupert L. L. KellnerAbstract:Abstract Biosynthesis of the structurally complex hemolymph toxin Pederin is an eminent character of Paederus females. For that capability, however, they rely on endosymbiotic bacteria that are lacking in aposymbiotic females. The bacterial inhabitants of the two phenotypes in Paederus sabaeus are evaluated in a PCR-based analysis of 16S rDNA. A certain fragment, which is not found in aposymbiotic females, is highly dominant in the other, biosynthesizing females and thus identifies the endosymbiont. Its DNA sequence reveals a member of the γ subdivision of the Proteobacteria that is clustered within the genus Pseudomonas (sensu stricto) as it is most closely related to Pseudomonas aeruginosa. These bacteria appear as the hypothesized common producers of Pederin and the Pederin family of analogs from marine sponges.
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horizontal transmission of biosynthetic capabilities for Pederin in paederus melanurus coleoptera staphylinidae
Chemoecology, 2001Co-Authors: Rupert L. L. KellnerAbstract:Biosynthesis of the defensive compound Pederin by females in the genus Paederus appears to be associated with endosymbiotic microorganisms. In P. melanurus, which could be maintained in the laboratory for several generations, horizontal transmission occurs by ingestion of eggs inoculated with the specific endosymbionts. They are responsible for females’ Pederin polymorphism. Heating or freezing eliminated or markedly reduced transmission. This indicates bacteria that are unable to produce endospores and lacking a cell wall. Influences other than Pederin biosynthesis could not be observed.
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suppression of Pederin biosynthesis through antibiotic elimination of endosymbionts in paederus sabaeus
Journal of Insect Physiology, 2001Co-Authors: Rupert L. L. KellnerAbstract:Biosynthesis of the unique defensive compound Pederin is confined to female rove beetles of the genus Paederus (Coleoptera: Staphylinidae). These (+)-females endow their eggs with toxin whereas (−)-females, which occur both naturally and in laboratory reared specimens, do not. The latter are aposymbionts lacking biosynthetic capabilities because of endosymbiotic deficiency. They can, however, be induced to accumulate Pederin if fed with (+)-eggs during larval development. The endosymbionts can thus be transmitted by ingestion of (+)-Eggs. (+)-eggs treated with benzylpenicillin, erythromycin, oxytetracycline or streptomycin show that the induction of Pederin accumulation depends on the antibiotic's spectrum of efficaciousness, its dosage and duration of the treatment. Certain bacteria, probably belonging to the gram-negative type, must be transmitted to produce (+)-females.
Bernhard Spengler - One of the best experts on this subject based on the ideXlab platform.
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metabolite localization by atmospheric pressure high resolution scanning microprobe matrix assisted laser desorption ionization mass spectrometry imaging in whole body sections and individual organs of the rove beetle paederus riparius
Analytical and Bioanalytical Chemistry, 2015Co-Authors: Dhaka Ram Bhandari, Matthias Schott, Andreas Rompp, Andreas Vilcinskas, Bernhard SpenglerAbstract:Mass spectrometry imaging provides for non-targeted, label-free chemical imaging. In this study, atmospheric pressure high-resolution scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI) was used for the first time to describe the chemical distribution of the defensive compounds Pederin, pseudoPederin, and pederon in tissue sections (16 μm thick) of the rove beetle Paederus riparius. The whole-insect tissue section was scanned with a 20-μm step size. Mass resolution of the orbital trapping mass spectrometer was set to 100,000 at m/z 200. Additionally, organ-specific compounds were identified for brain, nerve cord, eggs, gut, ovaries, and malpighian tubules. To confirm the distribution of the specific compounds, individual organs from the insect were dissected, and MSI experiments were performed on the dissected organs. Three ganglia of the nerve cord, with a dimension of 250–500 μm, were measured with 10-μm spatial resolution. High-quality m/z images, based on high spatial resolution and high mass accuracy were generated. These features helped to assign mass spectral peaks with high confidence. Mass accuracy of the imaging experiments was <3 ppm root mean square error, and mapping of different compound classes from a single experiment was possible. This approach improved the understanding of the biochemistry of P. riparius. Concentration differences and distributions of Pederin and its analogues could be visualized in the whole-insect section. Without any labeling, we assigned key lipids for specific organs to describe their location in the body and to identify morphological structures with a specificity higher than with staining or immunohistology methods.
Dhaka Ram Bhandari - One of the best experts on this subject based on the ideXlab platform.
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metabolite localization by atmospheric pressure high resolution scanning microprobe matrix assisted laser desorption ionization mass spectrometry imaging in whole body sections and individual organs of the rove beetle paederus riparius
Analytical and Bioanalytical Chemistry, 2015Co-Authors: Dhaka Ram Bhandari, Matthias Schott, Andreas Rompp, Andreas Vilcinskas, Bernhard SpenglerAbstract:Mass spectrometry imaging provides for non-targeted, label-free chemical imaging. In this study, atmospheric pressure high-resolution scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI) was used for the first time to describe the chemical distribution of the defensive compounds Pederin, pseudoPederin, and pederon in tissue sections (16 μm thick) of the rove beetle Paederus riparius. The whole-insect tissue section was scanned with a 20-μm step size. Mass resolution of the orbital trapping mass spectrometer was set to 100,000 at m/z 200. Additionally, organ-specific compounds were identified for brain, nerve cord, eggs, gut, ovaries, and malpighian tubules. To confirm the distribution of the specific compounds, individual organs from the insect were dissected, and MSI experiments were performed on the dissected organs. Three ganglia of the nerve cord, with a dimension of 250–500 μm, were measured with 10-μm spatial resolution. High-quality m/z images, based on high spatial resolution and high mass accuracy were generated. These features helped to assign mass spectral peaks with high confidence. Mass accuracy of the imaging experiments was <3 ppm root mean square error, and mapping of different compound classes from a single experiment was possible. This approach improved the understanding of the biochemistry of P. riparius. Concentration differences and distributions of Pederin and its analogues could be visualized in the whole-insect section. Without any labeling, we assigned key lipids for specific organs to describe their location in the body and to identify morphological structures with a specificity higher than with staining or immunohistology methods.
Andreas Rompp - One of the best experts on this subject based on the ideXlab platform.
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metabolite localization by atmospheric pressure high resolution scanning microprobe matrix assisted laser desorption ionization mass spectrometry imaging in whole body sections and individual organs of the rove beetle paederus riparius
Analytical and Bioanalytical Chemistry, 2015Co-Authors: Dhaka Ram Bhandari, Matthias Schott, Andreas Rompp, Andreas Vilcinskas, Bernhard SpenglerAbstract:Mass spectrometry imaging provides for non-targeted, label-free chemical imaging. In this study, atmospheric pressure high-resolution scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI) was used for the first time to describe the chemical distribution of the defensive compounds Pederin, pseudoPederin, and pederon in tissue sections (16 μm thick) of the rove beetle Paederus riparius. The whole-insect tissue section was scanned with a 20-μm step size. Mass resolution of the orbital trapping mass spectrometer was set to 100,000 at m/z 200. Additionally, organ-specific compounds were identified for brain, nerve cord, eggs, gut, ovaries, and malpighian tubules. To confirm the distribution of the specific compounds, individual organs from the insect were dissected, and MSI experiments were performed on the dissected organs. Three ganglia of the nerve cord, with a dimension of 250–500 μm, were measured with 10-μm spatial resolution. High-quality m/z images, based on high spatial resolution and high mass accuracy were generated. These features helped to assign mass spectral peaks with high confidence. Mass accuracy of the imaging experiments was <3 ppm root mean square error, and mapping of different compound classes from a single experiment was possible. This approach improved the understanding of the biochemistry of P. riparius. Concentration differences and distributions of Pederin and its analogues could be visualized in the whole-insect section. Without any labeling, we assigned key lipids for specific organs to describe their location in the body and to identify morphological structures with a specificity higher than with staining or immunohistology methods.
Matthias Schott - One of the best experts on this subject based on the ideXlab platform.
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metabolite localization by atmospheric pressure high resolution scanning microprobe matrix assisted laser desorption ionization mass spectrometry imaging in whole body sections and individual organs of the rove beetle paederus riparius
Analytical and Bioanalytical Chemistry, 2015Co-Authors: Dhaka Ram Bhandari, Matthias Schott, Andreas Rompp, Andreas Vilcinskas, Bernhard SpenglerAbstract:Mass spectrometry imaging provides for non-targeted, label-free chemical imaging. In this study, atmospheric pressure high-resolution scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI) was used for the first time to describe the chemical distribution of the defensive compounds Pederin, pseudoPederin, and pederon in tissue sections (16 μm thick) of the rove beetle Paederus riparius. The whole-insect tissue section was scanned with a 20-μm step size. Mass resolution of the orbital trapping mass spectrometer was set to 100,000 at m/z 200. Additionally, organ-specific compounds were identified for brain, nerve cord, eggs, gut, ovaries, and malpighian tubules. To confirm the distribution of the specific compounds, individual organs from the insect were dissected, and MSI experiments were performed on the dissected organs. Three ganglia of the nerve cord, with a dimension of 250–500 μm, were measured with 10-μm spatial resolution. High-quality m/z images, based on high spatial resolution and high mass accuracy were generated. These features helped to assign mass spectral peaks with high confidence. Mass accuracy of the imaging experiments was <3 ppm root mean square error, and mapping of different compound classes from a single experiment was possible. This approach improved the understanding of the biochemistry of P. riparius. Concentration differences and distributions of Pederin and its analogues could be visualized in the whole-insect section. Without any labeling, we assigned key lipids for specific organs to describe their location in the body and to identify morphological structures with a specificity higher than with staining or immunohistology methods.