The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Barry Ziola - One of the best experts on this subject based on the ideXlab platform.
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Susceptibility of Pediococcus isolates to antimicrobial compounds in relation to hop-resistance and beer-spoilage.
BMC microbiology, 2009Co-Authors: Monique Haakensen, David Vickers, Barry ZiolaAbstract:Background Though important in the context of food microbiology and as potential pathogens in immuno-compromised humans, bacterial isolates belonging to the genus Pediococcus are best known for their association with contamination of ethanol fermentation processes (beer, wine, or fuel ethanol). Use of antimicrobial compounds (e.g., hop-compounds, Penicillin) by some industries to combat Pediococcus contaminants is long-standing, yet knowledge about the resistance of pediococci to antimicrobial agents is minimal. Here we examined Pediococcus isolates to determine whether antibiotic resistance is associated with resistance to hops, presence of genes known to correlate with beer spoilage, or with ability to grow in beer.
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Phylogenetic analysis of the genus Pediococcus, including Pediococcus claussenii sp. nov., a novel lactic acid bacterium isolated from beer.
International journal of systematic and evolutionary microbiology, 2002Co-Authors: C Melissa Dobson, Harry Deneer, Sun Lee, Sean Hemmingsen, Sarah Glaze, Barry ZiolaAbstract:Pediococci are found in foods and on plants and as beer-spoilage agents. The goal of the present study was to use the DNA sequences of the first three variable regions of the 165 rRNA gene, the 16S-23S rRNA internally transcribed spacer region sequence and approximately a third of the 60 kDa heat-shock protein gene to elucidate phylogenetic groupings within the genus Pediococcus. Phylogenetic trees were created with sequence data from 31 Pediococcus and three Lactobacillus isolates. Complete 16S rRNA gene sequences from selected Pediococcus isolates were also examined. The results were interpreted in relation to the currently accepted Pediococcus species. We found that, where previously done, speciation of many Pediococcus isolates is inaccurate. Also, one grouping of seven isolates did not include any currently recognized Pediococcus species type isolate. Our phylogenetic analyses support the conclusion that these seven isolates, all of brewing spoilage origin, belong to a novel species, for which the name Pediococcus claussenii sp. nov. is proposed (type strain P06(T0 = ATCC BAA-344(T) = DSM 14800(T)). Phylogenetic analysis has therefore helped to resolve problems surrounding species identification of Pediococcus isolates.
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Immunoblotting Used for Identification of Beer Spoilage Pediococci, Including the New Species Pediococcus Claussenii1
Journal of the American Society of Brewing Chemists, 2002Co-Authors: Bonnie Chaban, Michael S. Whiting, C Melissa Dobson, Jaime Bjarnason, Barry ZiolaAbstract:Phenotype variability makes it difficult to speciate Pediococcus isolates. Through phylogenetic analyses, we recently determined that the genus Pediococcus contains six taxonomic groupings, includi...
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Bacterial surface antigen-specific monoclonal antibodies used to detect beer spoilage pediococci.
Canadian Journal of Microbiology, 1999Co-Authors: Michael S. Whiting, W.m. Ingledew, Barry ZiolaAbstract:Fourteen monoclonal antibodies (Mabs) were isolated that react with surface antigens of Pediococcus beer spoilage organisms, including P. damnosus, P. pentosaceous, P. acidilactici, and unspeciated isolates. Immunoblotting, enzyme immunoassays (EIAs) of protease- and neuraminidase-treated surface antigen extracts, carbohydrate competition EIAs, and cardiolipin EIAs were used to characterize the bacterial antigens involved in Mab binding. Antigen stability in situ was tested by protease treatment or surface antigen extraction of washed bacteria. In most cases, the Mabs bind to Pediococcus surface antigens that appear to be covalently bound cell wall polymers resistant to alteration or removal from the bacterial surface. These bacterial surface antigen reactive Mabs show good potential for rapid, sensitive, and specific immunoassay detection of Pediococcus beer spoilage organisms.
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bacterial surface antigen specific monoclonal antibodies used to detect beer spoilage pediococci
Canadian Journal of Microbiology, 1999Co-Authors: Michael S. Whiting, W.m. Ingledew, Barry ZiolaAbstract:Fourteen monoclonal antibodies (Mabs) were isolated that react with surface antigens of Pediococcus beer spoilage organisms, including P. damnosus, P. pentosaceous, P. acidilactici, and unspeciated isolates. Immunoblotting, enzyme immunoassays (EIAs) of protease- and neuraminidase-treated surface antigen extracts, carbohydrate competition EIAs, and cardiolipin EIAs were used to characterize the bacterial antigens involved in Mab binding. Antigen stability in situ was tested by protease treatment or surface antigen extraction of washed bacteria. In most cases, the Mabs bind to Pediococcus surface antigens that appear to be covalently bound cell wall polymers resistant to alteration or removal from the bacterial surface. These bacterial surface antigen reactive Mabs show good potential for rapid, sensitive, and specific immunoassay detection of Pediococcus beer spoilage organisms.Key words: beer spoilage organism, immunoassay, monoclonal antibodies, Pediococcus, surface antigens.
Xiuzhu Dong - One of the best experts on this subject based on the ideXlab platform.
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of the Chinese Academy of Sciences2, Chinese Academy of Sciences, Beijing 100080,
2016Co-Authors: Bin Zhang, Huichun Tong, Xiuzhu Dong, P. R. ChinaAbstract:Pediococcus cellicola sp. nov., a novel lactic acid coccus isolated from a distilled-spirit-fermenting cella
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Pediococcus ethanolidurans sp. nov., isolated from the walls of a distilled-spirit-fermenting cellar.
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Limeng Liu, Bin Zhang, Huichun Tong, Xiuzhu DongAbstract:Two novel Pediococcus strains, Z-9(T) and Z-17, were isolated from a distilled-spirit-fermenting cellar in Hebei Province, China. The cells were Gram-positive, non-spore-forming, non-motile cocci and occurred in pairs or tetrads. The strains were facultatively anaerobic and produced only lactic acid [d(-) and l(+) isomers in the ratio 50 : 50] from glucose fermentation. Catalase activity was not present. Both strains were able to grow in 6.5 % ethanol and at pH 3.5, but not in 4 % NaCl. The mean genomic G+C content of the two strains was 39.5+/-0.5 mol% (39 mol% for the type strain, Z-9(T)). The levels of 16S rRNA gene sequence similarity between the two novel strains and related species of the genus Pediococcus ranged from 98.3 to 98.7 %. The levels of DNA-DNA relatedness between strain Z-9(T) and the phylogenetically closely related pediococci Pediococcus damnosus LMG 11484(T), Pediococcus inopinatus LMG 11409(T), Pediococcus parvulus LMG 11486(T) and Pediococcus cellicola LMG 22956(T) were 14.6, 33, 28.7 and 16.8 %, respectively. On the basis of phenotypic, genotypic and phylogenetic analyses, a novel species, Pediococcus ethanolidurans sp. nov., is proposed, with strain Z-9(T) (=AS 1.3889(T)=LMG 23354(T)) as the type strain.
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Pediococcus cellicola sp nov a novel lactic acid coccus isolated from a distilled spirit fermenting cellar
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Bin Zhang, Huichun Tong, Xiuzhu DongAbstract:Two novel Pediococcus strains, Z-1 and Z-8 T , were isolated from a distilled-spirit-fermenting cellar in Hebei Province, China. The cells were Gram-positive, non-spore-forming, non-motile cocci, occurring in pairs or tetrads. The strains were facultatively anaerobic and produced 50% (”)-D-/50% (+)-L-lactic acid exclusively from glucose fermentation, but did not produce catalase. Both strains fermented rhamnose, ribose, xylose, lactose, maltose, trehalose and sucrose, and tolerated 10% ethanol. The mean G+C content of the two strains was 38±1 mol% (37 mol% for the type strain, Z-8 T ). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Z-8 T clustered within the Pediococcus damnosus–Pediococcus inopinatus–Pediococcus parvulus group, with 98?3–98?5% 16S rRNA gene similarity. DNA–DNA relatedness between strain Z-8 T and P. damnosus LMG 11484 T , P. inopinatus LMG 11409 T and P. parvulus LMG 11486 T was 19±4, 35±5 and 31±5%, respectively. Therefore, based on the distinctive phenotypic characteristics, and genotypic and phylogenetic analyses, a novel Pediococcus species, Pediococcus cellicola sp. nov., is proposed. The type strain is Z-8 T (=AS 1.3787 T =LMG 22956 T ).
Peter Vandamme - One of the best experts on this subject based on the ideXlab platform.
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Pediococcus lolii DSM 19927T and JCM 15055T are strains of Pediococcus acidilactici
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Anneleen Wieme, Ilse Cleenwerck, Anita Van Landschoot, Peter VandammeAbstract:Strain NGRI 0510QT, isolated from ryegrass silage, was recently classified as a representative of a novel Pediococcus species, Pediococcus lolii Doi et al. 2009. It was deposited in the DSMZ and JCM culture collections as DSM 19927T and JCM 15055T, respectively. A polyphasic taxonomic study, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, pheS and 16S rRNA gene sequence analysis, fluorescent amplified fragment length polymorphism and DNA–DNA hybridization, was used to prove that both subcultures of the type, and only, strain of this species are strains of Pediococcus acidilactici .
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Pediococcus lolii DSM 19927T and JCM 15055T are strains of Pediococcus acidilactici.
International journal of systematic and evolutionary microbiology, 2012Co-Authors: Anneleen Wieme, Ilse Cleenwerck, Anita Van Landschoot, Peter VandammeAbstract:Strain NGRI 0510Q(T), isolated from ryegrass silage, was recently classified as a representative of a novel Pediococcus species, Pediococcus lolii Doi et al. 2009. It was deposited in the DSMZ and JCM culture collections as DSM 19927(T) and JCM 15055(T), respectively. A polyphasic taxonomic study, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, pheS and 16S rRNA gene sequence analysis, fluorescent amplified fragment length polymorphism and DNA-DNA hybridization, was used to prove that both subcultures of the type, and only, strain of this species are strains of Pediococcus acidilactici.
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Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis.
International journal of systematic and evolutionary microbiology, 2008Co-Authors: Katrien De Bruyne, Charles M A P Franz, Marc Vancanneyt, Ulrich Schillinger, Fernanda Mozzi, Graciela Font De Valdez, Luc De Vuyst, Peter VandammeAbstract:A Gram-positive, small coccus-shaped lactic acid bacterium, strain LMG 23999(T), was isolated from Argentinean wheat flour. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus Pediococcus, with Pediococcus stilesii, Pediococcus pentosaceus and Pediococcus acidilactici as its closest relatives (97.7, 97.3 and 96.9 % gene sequence similarity, respectively). Fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and whole-cell protein electrophoresis confirmed the unique taxonomic status of the novel strain. DNA-DNA hybridizations, DNA G+C content determination, comparative sequence analysis of the pheS, rpoA and atpA genes and physiological and biochemical characterization demonstrated that strain LMG 23999(T) (=CCUG 54535(T)=CRL 776(T)) represents a novel species for which the name Pediococcus argentinicus sp. nov. is proposed. Multi-locus sequence analysis based on pheS, rpoA and atpA genes was found to be a suitable method for the identification of species of the genus Pediococcus.
Michael S. Whiting - One of the best experts on this subject based on the ideXlab platform.
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Immunoblotting Used for Identification of Beer Spoilage Pediococci, Including the New Species Pediococcus Claussenii1
Journal of the American Society of Brewing Chemists, 2002Co-Authors: Bonnie Chaban, Michael S. Whiting, C Melissa Dobson, Jaime Bjarnason, Barry ZiolaAbstract:Phenotype variability makes it difficult to speciate Pediococcus isolates. Through phylogenetic analyses, we recently determined that the genus Pediococcus contains six taxonomic groupings, includi...
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Bacterial surface antigen-specific monoclonal antibodies used to detect beer spoilage pediococci.
Canadian Journal of Microbiology, 1999Co-Authors: Michael S. Whiting, W.m. Ingledew, Barry ZiolaAbstract:Fourteen monoclonal antibodies (Mabs) were isolated that react with surface antigens of Pediococcus beer spoilage organisms, including P. damnosus, P. pentosaceous, P. acidilactici, and unspeciated isolates. Immunoblotting, enzyme immunoassays (EIAs) of protease- and neuraminidase-treated surface antigen extracts, carbohydrate competition EIAs, and cardiolipin EIAs were used to characterize the bacterial antigens involved in Mab binding. Antigen stability in situ was tested by protease treatment or surface antigen extraction of washed bacteria. In most cases, the Mabs bind to Pediococcus surface antigens that appear to be covalently bound cell wall polymers resistant to alteration or removal from the bacterial surface. These bacterial surface antigen reactive Mabs show good potential for rapid, sensitive, and specific immunoassay detection of Pediococcus beer spoilage organisms.
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bacterial surface antigen specific monoclonal antibodies used to detect beer spoilage pediococci
Canadian Journal of Microbiology, 1999Co-Authors: Michael S. Whiting, W.m. Ingledew, Barry ZiolaAbstract:Fourteen monoclonal antibodies (Mabs) were isolated that react with surface antigens of Pediococcus beer spoilage organisms, including P. damnosus, P. pentosaceous, P. acidilactici, and unspeciated isolates. Immunoblotting, enzyme immunoassays (EIAs) of protease- and neuraminidase-treated surface antigen extracts, carbohydrate competition EIAs, and cardiolipin EIAs were used to characterize the bacterial antigens involved in Mab binding. Antigen stability in situ was tested by protease treatment or surface antigen extraction of washed bacteria. In most cases, the Mabs bind to Pediococcus surface antigens that appear to be covalently bound cell wall polymers resistant to alteration or removal from the bacterial surface. These bacterial surface antigen reactive Mabs show good potential for rapid, sensitive, and specific immunoassay detection of Pediococcus beer spoilage organisms.Key words: beer spoilage organism, immunoassay, monoclonal antibodies, Pediococcus, surface antigens.
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Detection of Pediococcus spp. in brewing yeast by a rapid immunoassay.
Applied and environmental microbiology, 1992Co-Authors: Michael S. Whiting, W.m. Ingledew, M Crichlow, Barry ZiolaAbstract:A membrane immunofluorescent-antibody test was developed to detect diacetyl-producing Pediococcus contaminants in brewery pitching yeast (yeast [Saccharomyces cerevisiae] slurry collected for reinoculation). Centrifugations at 11 and 5,100 x g separate yeast cells from bacteria and concentrate the bacteria, respectively. Pelleted bacteria resuspended and trapped on a black membrane filter are reacted with monoclonal antibodies specific for cell surface antigens and then with fluorescein-conjugated indicator antibodies. Whether pitching yeast is contaminated with pediococci at 0.001% is determined in less than 4 h. The sensitivity of the assay is 2 orders of magnitude below the Pediococcus detection limit of direct microscopy. Images
Bin Zhang - One of the best experts on this subject based on the ideXlab platform.
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of the Chinese Academy of Sciences2, Chinese Academy of Sciences, Beijing 100080,
2016Co-Authors: Bin Zhang, Huichun Tong, Xiuzhu Dong, P. R. ChinaAbstract:Pediococcus cellicola sp. nov., a novel lactic acid coccus isolated from a distilled-spirit-fermenting cella
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Pediococcus ethanolidurans sp. nov., isolated from the walls of a distilled-spirit-fermenting cellar.
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Limeng Liu, Bin Zhang, Huichun Tong, Xiuzhu DongAbstract:Two novel Pediococcus strains, Z-9(T) and Z-17, were isolated from a distilled-spirit-fermenting cellar in Hebei Province, China. The cells were Gram-positive, non-spore-forming, non-motile cocci and occurred in pairs or tetrads. The strains were facultatively anaerobic and produced only lactic acid [d(-) and l(+) isomers in the ratio 50 : 50] from glucose fermentation. Catalase activity was not present. Both strains were able to grow in 6.5 % ethanol and at pH 3.5, but not in 4 % NaCl. The mean genomic G+C content of the two strains was 39.5+/-0.5 mol% (39 mol% for the type strain, Z-9(T)). The levels of 16S rRNA gene sequence similarity between the two novel strains and related species of the genus Pediococcus ranged from 98.3 to 98.7 %. The levels of DNA-DNA relatedness between strain Z-9(T) and the phylogenetically closely related pediococci Pediococcus damnosus LMG 11484(T), Pediococcus inopinatus LMG 11409(T), Pediococcus parvulus LMG 11486(T) and Pediococcus cellicola LMG 22956(T) were 14.6, 33, 28.7 and 16.8 %, respectively. On the basis of phenotypic, genotypic and phylogenetic analyses, a novel species, Pediococcus ethanolidurans sp. nov., is proposed, with strain Z-9(T) (=AS 1.3889(T)=LMG 23354(T)) as the type strain.
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Pediococcus cellicola sp nov a novel lactic acid coccus isolated from a distilled spirit fermenting cellar
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Bin Zhang, Huichun Tong, Xiuzhu DongAbstract:Two novel Pediococcus strains, Z-1 and Z-8 T , were isolated from a distilled-spirit-fermenting cellar in Hebei Province, China. The cells were Gram-positive, non-spore-forming, non-motile cocci, occurring in pairs or tetrads. The strains were facultatively anaerobic and produced 50% (”)-D-/50% (+)-L-lactic acid exclusively from glucose fermentation, but did not produce catalase. Both strains fermented rhamnose, ribose, xylose, lactose, maltose, trehalose and sucrose, and tolerated 10% ethanol. The mean G+C content of the two strains was 38±1 mol% (37 mol% for the type strain, Z-8 T ). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Z-8 T clustered within the Pediococcus damnosus–Pediococcus inopinatus–Pediococcus parvulus group, with 98?3–98?5% 16S rRNA gene similarity. DNA–DNA relatedness between strain Z-8 T and P. damnosus LMG 11484 T , P. inopinatus LMG 11409 T and P. parvulus LMG 11486 T was 19±4, 35±5 and 31±5%, respectively. Therefore, based on the distinctive phenotypic characteristics, and genotypic and phylogenetic analyses, a novel Pediococcus species, Pediococcus cellicola sp. nov., is proposed. The type strain is Z-8 T (=AS 1.3787 T =LMG 22956 T ).
