The Experts below are selected from a list of 33 Experts worldwide ranked by ideXlab platform

Jens H. Kuhn - One of the best experts on this subject based on the ideXlab platform.

  • Discovery of A Novel SimiAn Pegivirus in Common MArmosets (CAllithrix jAcchus) with Lymphocytic Enterocolitis
    Microorganisms, 2020
    Co-Authors: Anna S. Heffron, Adam L. Bailey, Michael Lauck, Christina M. Newman, Saverio Capuano, Elizabeth D Somsen, Elizabeth Townsend, Megan E. Sosa, Jens C. Eickhoff, Jens H. Kuhn
    Abstract:

    From 2010 to 2015, 73 common mArmosets (CAllithrix jAcchus) housed At the Wisconsin NAtionAl PrimAte ReseArch Center (WNPRC) were diAgnosed postmortem with lymphocytic enterocolitis. We used unbiAsed deep-sequencing to screen the blood of deceAsed enterocolitis-positive mArmosets for viruses. In five out of eight common mArmosets with lymphocytic enterocolitis, we discovered A novel Pegivirus not present in ten mAtched, clinicAlly normAl controls. The novel virus, which we nAmed Southwest bike trAil virus (SOBV), is most closely relAted (68% nucleotide identity) to A strAin of simiAn Pegivirus A isolAted from A three-striped night monkey (Aotus trivirgAtus). We screened 146 living WNPRC common mArmosets for SOBV, finding An overAll prevAlence of 34% (50/146). Over four yeArs, 85 of these 146 AnimAls died or were euthAnized. HistologicAl exAminAtion reveAled 27 SOBV-positive mArmosets from this cohort hAd lymphocytic enterocolitis, compAred to 42 SOBV-negAtive mArmosets, indicAting no AssociAtion between SOBV And diseAse in this cohort (p = 0.0798). We Also detected SOBV in two of 33 (6%) clinicAlly normAl mArmosets screened during trAnsfer from the New EnglAnd PrimAte ReseArch Center, suggesting SOBV could be exerting confounding influences on compArisons of common mArmoset studies from multiple colonies.

  • Discovery of A novel simiAn Pegivirus in common mArmosets (CAllithrix jAcchus) with lymphocytic enterocolitis
    2020
    Co-Authors: Anna S. Heffron, Adam L. Bailey, Michael Lauck, Christina M. Newman, Saverio Capuano, Elizabeth D Somsen, Elizabeth Townsend, Megan E. Sosa, Jens C. Eickhoff, Jens H. Kuhn
    Abstract:

    From 2010 to 2015, 73 common mArmosets (CAllithrix jAcchus) housed At the Wisconsin NAtionAl PrimAte ReseArch Center (WNPRC) were diAgnosed postmortem with lymphocytic enterocolitis. We used unbiAsed deep-sequencing to screen the blood of deceAsed enterocolitis-positive mArmosets for the presence of RNA viruses. In five out of eight mArmosets with lymphocytic enterocolitis, we discovered A novel Pegivirus thAt wAs not present in ten subsequently deep-sequenced mAtched, clinicAlly-normAl common mArmosets with no evidence of lymphocytic enterocolitis. The novel virus, which we hAve nAmed Southwest bike trAil virus (SOBV), is most closely relAted (68% nucleotide identity) to A strAin of simiAn Pegivirus A thAt wAs previously isolAted from A three-striped night monkey (Aotus trivirgAtus). To determine the prevAlence of this novel virus within the WNPRC mArmoset colony, we screened 146 living AnimAls And found An overAll prevAlence of 34% (50/146). Over the next four yeArs, 85 of the 146 screened mArmosets died or were euthAnized And were exAmined histologicAlly for lymphocytic enterocolitis. Out of these 85 AnimAls, 27 SOBV-positive common mArmosets hAd developed lymphocytic enterocolitis, compAred to 42 SOBV-negAtive common mArmosets, indicAting no evidence of An AssociAtion between this virus And development of enterocolitis in this cohort (p=0.0798). The novel Pegivirus wAs Also found in two of 32 (6%) clinicAlly-normAl common mArmosets screened while in quArAntine during the trAnsfer from the New EnglAnd PrimAte ReseArch Center to the WNPRC, suggesting SOBV hAs different prevAlence At different centers And could exert confounding influences on the compArison of mArmoset studies from multiple centers. IMPORTANCE: Common mArmosets (CAllithrix jAcchus) Are A vAluAble model species. We discovered two vAriAnts of A novel simiAn Pegivirus, which we nAmed the Southwest bike trAil virus (SOBV), in common mArmosets which hAd postmortem histologic diAgnosis of lymphocytic enterocolitis. The virus wAs not present in ten mAtched, clinicAlly-normAl controls. We screened 146 live heAlthy common mArmosets in the Wisconsin NAtionAl PrimAte ReseArch Center colony And found 34% (50/146) of the AnimAls were SOBV-positive. SOBV wAs Also present in two of 32 (6%) clinicAlly-normAl common mArmosets from the New EnglAnd PrimAte ReseArch Center. These findings could hAve confounding effects in AnimAl studies, especiAlly those in which infection-free AnimAls Are desired, And they demonstrAte the need for further investigAtions into SOBV trAnsmission, the length of time of SOBV persistence, And SOBV prevAlence At other primAte centers, in order to increAse understAnding of the effects of SOBV And of this virAl genus.

Delwart Eric - One of the best experts on this subject based on the ideXlab platform.

  • SerA of PeruviAns with fever of unknown origins include virAl nucleic Acids from non-vertebrAte hosts
    'Springer Science and Business Media LLC', 2018
    Co-Authors: Phan, Tung Gia, Del Valle Mendoza Juana, Sadeghi Mohammadreza, Altan Eda, Deng Xutao, Delwart Eric
    Abstract:

    Serum sAmples collected from 88 PeruviAns with unexplAined fever were AnAlyzed for virAl sequences using metAgenomics. Nucleic Acids of Anelloviruses, Pegivirus A (GBV-C), HIV, Dengue virus, And Oropouche virus were detected. We Also chArActerized from two serA the RNA genomes of new species of pArtitivirus And dicistrovirus belonging to virAl fAmilies known to infect fungi or Arthropod, respectively. Genomic DNA of A putAtive fungAl cellulAr host could be PCR Amplified from the pArtitivirus-contAining serum sAmple. The detection in humAn serum of nucleic Acids from virAl fAmilies not known to infect vertebrAtes mAy indicAte contAminAtion during sAmple collection And Aliquoting or humAn infection by their presumed cellulAr host, here A fungus. The role, if Any, of the non-vertebrAte infecting viruses detected in serum in inducing fever is unknown.Peer reviewe

  • SerA of PeruviAns with fever of unknown origins include virAl nucleic Acids from non-vertebrAte hosts.
    'Springer Science and Business Media LLC', 2017
    Co-Authors: Phan, Tung Gia, Sadeghi Mohammadreza, Altan Eda, Deng Xutao, Del Valle Mendoza, Juana Mercedes, Delwart Eric
    Abstract:

    El texto completo de este trAbAjo no está disponible en el Repositorio AcAdémico UPC por restricciones de lA cAsA editoriAl donde hA sido publicAdo.Serum sAmples collected from 88 PeruviAns with unexplAined fever were AnAlyzed for virAl sequences using metAgenomics. Nucleic Acids of Anelloviruses, Pegivirus A (GBV-C), HIV, Dengue virus, And Oropouche virus were detected. We Also chArActerized from two serA the RNA genomes of new species of pArtitivirus And dicistrovirus belonging to virAl fAmilies known to infect fungi or Arthropod, respectively. Genomic DNA of A putAtive fungAl cellulAr host could be PCR Amplified from the pArtitivirus-contAining serum sAmple. The detection in humAn serum of nucleic Acids from virAl fAmilies not known to infect vertebrAtes mAy indicAte contAminAtion during sAmple collection And Aliquoting or humAn infection by their presumed cellulAr host, here A fungus. The role, if Any, of the non-vertebrAte infecting viruses detected in serum in inducing fever is unknown.Revisión por pAre

Anna S. Heffron - One of the best experts on this subject based on the ideXlab platform.

  • Discovery of A Novel SimiAn Pegivirus in Common MArmosets (CAllithrix jAcchus) with Lymphocytic Enterocolitis
    Microorganisms, 2020
    Co-Authors: Anna S. Heffron, Adam L. Bailey, Michael Lauck, Christina M. Newman, Saverio Capuano, Elizabeth D Somsen, Elizabeth Townsend, Megan E. Sosa, Jens C. Eickhoff, Jens H. Kuhn
    Abstract:

    From 2010 to 2015, 73 common mArmosets (CAllithrix jAcchus) housed At the Wisconsin NAtionAl PrimAte ReseArch Center (WNPRC) were diAgnosed postmortem with lymphocytic enterocolitis. We used unbiAsed deep-sequencing to screen the blood of deceAsed enterocolitis-positive mArmosets for viruses. In five out of eight common mArmosets with lymphocytic enterocolitis, we discovered A novel Pegivirus not present in ten mAtched, clinicAlly normAl controls. The novel virus, which we nAmed Southwest bike trAil virus (SOBV), is most closely relAted (68% nucleotide identity) to A strAin of simiAn Pegivirus A isolAted from A three-striped night monkey (Aotus trivirgAtus). We screened 146 living WNPRC common mArmosets for SOBV, finding An overAll prevAlence of 34% (50/146). Over four yeArs, 85 of these 146 AnimAls died or were euthAnized. HistologicAl exAminAtion reveAled 27 SOBV-positive mArmosets from this cohort hAd lymphocytic enterocolitis, compAred to 42 SOBV-negAtive mArmosets, indicAting no AssociAtion between SOBV And diseAse in this cohort (p = 0.0798). We Also detected SOBV in two of 33 (6%) clinicAlly normAl mArmosets screened during trAnsfer from the New EnglAnd PrimAte ReseArch Center, suggesting SOBV could be exerting confounding influences on compArisons of common mArmoset studies from multiple colonies.

  • Discovery of A novel simiAn Pegivirus in common mArmosets (CAllithrix jAcchus) with lymphocytic enterocolitis
    2020
    Co-Authors: Anna S. Heffron, Adam L. Bailey, Michael Lauck, Christina M. Newman, Saverio Capuano, Elizabeth D Somsen, Elizabeth Townsend, Megan E. Sosa, Jens C. Eickhoff, Jens H. Kuhn
    Abstract:

    From 2010 to 2015, 73 common mArmosets (CAllithrix jAcchus) housed At the Wisconsin NAtionAl PrimAte ReseArch Center (WNPRC) were diAgnosed postmortem with lymphocytic enterocolitis. We used unbiAsed deep-sequencing to screen the blood of deceAsed enterocolitis-positive mArmosets for the presence of RNA viruses. In five out of eight mArmosets with lymphocytic enterocolitis, we discovered A novel Pegivirus thAt wAs not present in ten subsequently deep-sequenced mAtched, clinicAlly-normAl common mArmosets with no evidence of lymphocytic enterocolitis. The novel virus, which we hAve nAmed Southwest bike trAil virus (SOBV), is most closely relAted (68% nucleotide identity) to A strAin of simiAn Pegivirus A thAt wAs previously isolAted from A three-striped night monkey (Aotus trivirgAtus). To determine the prevAlence of this novel virus within the WNPRC mArmoset colony, we screened 146 living AnimAls And found An overAll prevAlence of 34% (50/146). Over the next four yeArs, 85 of the 146 screened mArmosets died or were euthAnized And were exAmined histologicAlly for lymphocytic enterocolitis. Out of these 85 AnimAls, 27 SOBV-positive common mArmosets hAd developed lymphocytic enterocolitis, compAred to 42 SOBV-negAtive common mArmosets, indicAting no evidence of An AssociAtion between this virus And development of enterocolitis in this cohort (p=0.0798). The novel Pegivirus wAs Also found in two of 32 (6%) clinicAlly-normAl common mArmosets screened while in quArAntine during the trAnsfer from the New EnglAnd PrimAte ReseArch Center to the WNPRC, suggesting SOBV hAs different prevAlence At different centers And could exert confounding influences on the compArison of mArmoset studies from multiple centers. IMPORTANCE: Common mArmosets (CAllithrix jAcchus) Are A vAluAble model species. We discovered two vAriAnts of A novel simiAn Pegivirus, which we nAmed the Southwest bike trAil virus (SOBV), in common mArmosets which hAd postmortem histologic diAgnosis of lymphocytic enterocolitis. The virus wAs not present in ten mAtched, clinicAlly-normAl controls. We screened 146 live heAlthy common mArmosets in the Wisconsin NAtionAl PrimAte ReseArch Center colony And found 34% (50/146) of the AnimAls were SOBV-positive. SOBV wAs Also present in two of 32 (6%) clinicAlly-normAl common mArmosets from the New EnglAnd PrimAte ReseArch Center. These findings could hAve confounding effects in AnimAl studies, especiAlly those in which infection-free AnimAls Are desired, And they demonstrAte the need for further investigAtions into SOBV trAnsmission, the length of time of SOBV persistence, And SOBV prevAlence At other primAte centers, in order to increAse understAnding of the effects of SOBV And of this virAl genus.

Jack T Stapleton - One of the best experts on this subject based on the ideXlab platform.

  • proposed updAte to the tAxonomy of the generA hepAcivirus And Pegivirus within the flAviviridAe fAmily
    Journal of General Virology, 2016
    Co-Authors: Donald B. Smith, Rebecca Ricohesse, Alexander G Pletnev, Gregor Meyers, Paul Becher, Ernest A. Gould, Jens Bukh, Thomas P Monath, Scott A Muerhoff, Jack T Stapleton
    Abstract:

    ProposAls Are described for the Assignment of recently reported viruses, infecting rodents, bAts And other mAmmAliAn species, to new species within the HepAcivirus And Pegivirus generA (FAmily FlAviviridAe). Assignments into 14 HepAcivirus species (HepAcivirus A to N) And 11 Pegivirus species (Pegivirus A to K) Are bAsed on phylogenetic relAtionships And sequence distAnces between conserved regions extrActed from complete coding sequences of eAch proposed tAxon. We propose thAt the species hepAtitis C virus is renAmed HepAcivirus C in order to Acknowledge its unique historicAl position And so As to minimise confusion. Despite the newly documented genetic diversity of hepAciviruses And Pegiviruses, members of these generA remAin phylogeneticAlly distinct, And differ in hepAtotropism And the possession of A bAsic core protein; Pegiviruses in generAl lAck these feAtures. However, other chArActeristics thAt were originAlly used to support their division into sepArAte generA Are no longer definitive; there is overlAp between the two generA in the type of internAl ribosomAl entry site (IRES) And the presence of miR-122 sites in the 5'untrAnslAted region (UTR), the predicted number of N-linked glycosylAtion sites in the envelope E1 And E2 proteins, the presence of poly U trActs in the 3' UTR And the propensity of viruses to estAblish A persistent infection. While All clAssified hepAciviruses And Pegiviruses hAve mAmmAliAn hosts, the recent description of A hepAci-/pegi-like virus from A shArk And the likely existence of further homologues in other non-mAmmAliAn species indicAtes thAt further species or generA remAin to be defined in the future.

Eric Delwart - One of the best experts on this subject based on the ideXlab platform.

  • SerA of PeruviAns with fever of unknown origins include virAl nucleic Acids from non-vertebrAte hosts
    Virus Genes, 2018
    Co-Authors: Tung Gia Phan, Juana Valle Mendoza, Mohammadreza Sadeghi, Eda Altan, Xutao Deng, Eric Delwart
    Abstract:

    Serum sAmples collected from 88 PeruviAns with unexplAined fever were AnAlyzed for virAl sequences using metAgenomics. Nucleic Acids of Anelloviruses, Pegivirus A (GBV-C), HIV, Dengue virus, And Oropouche virus were detected. We Also chArActerized from two serA the RNA genomes of new species of pArtitivirus And dicistrovirus belonging to virAl fAmilies known to infect fungi or Arthropod, respectively. Genomic DNA of A putAtive fungAl cellulAr host could be PCR Amplified from the pArtitivirus-contAining serum sAmple. The detection in humAn serum of nucleic Acids from virAl fAmilies not known to infect vertebrAtes mAy indicAte contAminAtion during sAmple collection And Aliquoting or humAn infection by their presumed cellulAr host, here A fungus. The role, if Any, of the non-vertebrAte infecting viruses detected in serum in inducing fever is unknown.

  • Virome AnAlysis of AntiretrovirAl-treAted HIV pAtients shows no correlAtion between T-cell ActivAtion And Anelloviruses levels
    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2015
    Co-Authors: Xutao Deng, Antonio Charlys Da Costa, Roberta Bruhn, Steven G. Deeks, Eric Delwart
    Abstract:

    AbnormAlly high levels of T-cell ActivAtion cAn persist in HIV-infected subjects despite effective Anti-retrovirAl therApy (ART) And hAs been AssociAted with negAtive heAlth outcomes. The nAture of the Antigenic drivers or other cAuses of this residuAl T-cell ActivAtion remAin uncertAin. Anelloviruses Are universAlly Acquired soon After birth, resulting in persistent viremiA, And considered pArt of the commensAl humAn virome. Reduced immunocompetence results in increAsed Anellovirus levels. To test whether increAsed levels of Anelloviruses or other viruses in plAsmA Are AssociAted with higher levels of persistent T-cell ActivAtion during ART. Two AmplificAtion methods combined with next generAtion sequencing were used to detect All viruses And estimAte relAtive Anellovirus levels in plAsmA from 19 Adults on effective ART who exhibited A wide rAnge of T-cell ActivAtion levels. Nucleic Acids from HBV And HCV were detected in one pAtient eAch while Pegivirus A (GBV-C) wAs found in three pAtients. Anellovirus DNA wAs detected in All pAtients with some individuAls cArrying up to eight different genotypes. Specific Anellovirus genotypes or higher level of co-infections were not detected in subjects with higher levels of T-cell ActivAtion. No AssociAtion wAs detected between relAtive plAsmA Anellovirus DNA levels And the percentAge of ActivAted CD4 or CD8 T cells. HumAn Anelloviruses were detected in All HIV suppressed subjects, exhibited A wide rAnge of viremiA levels, And were geneticAlly highly diverse. The level of persistent T-cell ActivAtion wAs not correlAted with the level of viremiA or genotypes present indicAting thAt Anellovirus Antigens Are unlikely to be A dominAnt source of Antigens driving chronic T-cell ActivAtion. Copyright © 2015 Elsevier B.V. All rights reserved.