The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform
Rauri C. K. Bowie - One of the best experts on this subject based on the ideXlab platform.
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Development and characterization of microsatellite loci from the Great White Pelican (Pelecanus onocrotalus) and widespread application to other members of the Pelecanidae
Conservation Genetics, 2009Co-Authors: Marta Ponte Machado, Kevin A. Feldheim, Anna B. Sellas, Rauri C. K. BowieAbstract:We describe the isolation of 10 microsatellite loci from the Great White Pelican using an enrichment protocol. All loci were variable with the number of alleles ranging from 2 to 19, and observed heterozygosity ranging from 0.261 to 0.913. Two loci were out of Hardy–Weinberg equilibrium, although in each case this was restricted to one of the two populations screened. None of the loci were linked. Eight to 10 loci PCR-amplified in three related species: Brown, American White and Pink-backed Pelicans. These loci should prove to be widely applicable to studies of phylogeography and demography in pelicans globally.
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Development and characterization of microsatellite loci from the Great White Pelican ( Pelecanus onocrotalus ) and widespread application to other members of the Pelecanidae
Conservation Genetics, 2008Co-Authors: Marta Ponte Machado, Kevin A. Feldheim, Anna B. Sellas, Rauri C. K. BowieAbstract:We describe the isolation of 10 microsatellite loci from the Great White Pelican using an enrichment protocol. All loci were variable with the number of alleles ranging from 2 to 19, and observed heterozygosity ranging from 0.261 to 0.913. Two loci were out of Hardy–Weinberg equilibrium, although in each case this was restricted to one of the two populations screened. None of the loci were linked. Eight to 10 loci PCR-amplified in three related species: Brown, American White and Pink-backed Pelicans. These loci should prove to be widely applicable to studies of phylogeography and demography in pelicans globally.
Marta Ponte Machado - One of the best experts on this subject based on the ideXlab platform.
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Development and characterization of microsatellite loci from the Great White Pelican (Pelecanus onocrotalus) and widespread application to other members of the Pelecanidae
Conservation Genetics, 2009Co-Authors: Marta Ponte Machado, Kevin A. Feldheim, Anna B. Sellas, Rauri C. K. BowieAbstract:We describe the isolation of 10 microsatellite loci from the Great White Pelican using an enrichment protocol. All loci were variable with the number of alleles ranging from 2 to 19, and observed heterozygosity ranging from 0.261 to 0.913. Two loci were out of Hardy–Weinberg equilibrium, although in each case this was restricted to one of the two populations screened. None of the loci were linked. Eight to 10 loci PCR-amplified in three related species: Brown, American White and Pink-backed Pelicans. These loci should prove to be widely applicable to studies of phylogeography and demography in pelicans globally.
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Development and characterization of microsatellite loci from the Great White Pelican ( Pelecanus onocrotalus ) and widespread application to other members of the Pelecanidae
Conservation Genetics, 2008Co-Authors: Marta Ponte Machado, Kevin A. Feldheim, Anna B. Sellas, Rauri C. K. BowieAbstract:We describe the isolation of 10 microsatellite loci from the Great White Pelican using an enrichment protocol. All loci were variable with the number of alleles ranging from 2 to 19, and observed heterozygosity ranging from 0.261 to 0.913. Two loci were out of Hardy–Weinberg equilibrium, although in each case this was restricted to one of the two populations screened. None of the loci were linked. Eight to 10 loci PCR-amplified in three related species: Brown, American White and Pink-backed Pelicans. These loci should prove to be widely applicable to studies of phylogeography and demography in pelicans globally.
Roger S. Nasci - One of the best experts on this subject based on the ideXlab platform.
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Vector competence of the stable fly (Diptera: Muscidae) for West Nile virus.
Journal of medical entomology, 2011Co-Authors: Michael S. Doyle, Bethany N. Swope, Jerome A. Hogsette, Kristen L. Burkhalter, Harry M. Savage, Roger S. NasciAbstract:ABSTRACT In 2006–2007, stable flies, Stomoxys calcitrans (L.) (Diptera: Muscidae), were suspected of being enzootic vectors of West Nile virus (family Flaviviridae, genus Flavivirus, WNV) during a die-off of American white pelicans (Pelecanus erythrorhynchos Gmelin) (Pelecanidae) in Montana, USA. WNV-positive stable flies were observed feeding en masse on incapacitated, WNV-positive pelicans, arousing suspicions that the flies could have been involved in WNV transmission among pelicans, and perhaps to livestock and humans. We assessed biological transmission by infecting stable flies intrathoracically with WNV and testing them at 2-d intervals over 20 d. Infectious WNV was detected in fly bodies in decreasing amounts over time for only the first 6 d postinfection, an indication that WNV did not replicate within fly tissues and that stable flies cannot biologically transmit WNV. We assessed mechanical transmission using a novel technique. Specifically, we fed WNV-infected blood to individual flies by using...
Nicholas Komar - One of the best experts on this subject based on the ideXlab platform.
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Detection of West Nile virus in stable flies (Diptera: Muscidae) parasitizing juvenile American white pelicans.
Journal of medical entomology, 2010Co-Authors: Gregory D. Johnson, Nicholas A. Panella, Kristina Hale, Nicholas KomarAbstract:Stable flies, Stomoxys calcitrans (L.) (Diptera: Muscidae), an economically important pest of livestock and humans, were observed parasitizing prefledged American white pelicans, Pelecanus erythrorhynchos (Pelecaniformes: Pelecanidae), in a pelican breeding colony in northeastern Montana where die-offs attributed to West Nile virus (family Flaviviridae, genus Flavivirus, WNV) have occurred since 2002. Engorged and unengorged flies were collected off nine moribund chicks. Of 29 blood-engorged flies testing positive for vertebrate DNA, all 29 contained pelican DNA. Virus isolation was performed on 60 pools (1,176 flies) of unengorged flies using Vero cell plaque assay. Eighteen pools were positive for WNV for an estimated infection rate of 18.0 per 1,000 flies. Fifty-four percent (36/67) of abdomens from blood-engorged flies tested positive for WNV. Pelican viremia levels from the blood-engorged fly abdomens revealed that at least one of the ill pelicans circulated a viremia capable of infecting Culex mosquito vectors. Stable flies may be involved in WNV transmission within the pelican breeding colony by serving as either a mechanical vector or as a source for oral infection if ingested by predators.
Kevin A. Feldheim - One of the best experts on this subject based on the ideXlab platform.
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Development and characterization of microsatellite loci from the Great White Pelican (Pelecanus onocrotalus) and widespread application to other members of the Pelecanidae
Conservation Genetics, 2009Co-Authors: Marta Ponte Machado, Kevin A. Feldheim, Anna B. Sellas, Rauri C. K. BowieAbstract:We describe the isolation of 10 microsatellite loci from the Great White Pelican using an enrichment protocol. All loci were variable with the number of alleles ranging from 2 to 19, and observed heterozygosity ranging from 0.261 to 0.913. Two loci were out of Hardy–Weinberg equilibrium, although in each case this was restricted to one of the two populations screened. None of the loci were linked. Eight to 10 loci PCR-amplified in three related species: Brown, American White and Pink-backed Pelicans. These loci should prove to be widely applicable to studies of phylogeography and demography in pelicans globally.
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Development and characterization of microsatellite loci from the Great White Pelican ( Pelecanus onocrotalus ) and widespread application to other members of the Pelecanidae
Conservation Genetics, 2008Co-Authors: Marta Ponte Machado, Kevin A. Feldheim, Anna B. Sellas, Rauri C. K. BowieAbstract:We describe the isolation of 10 microsatellite loci from the Great White Pelican using an enrichment protocol. All loci were variable with the number of alleles ranging from 2 to 19, and observed heterozygosity ranging from 0.261 to 0.913. Two loci were out of Hardy–Weinberg equilibrium, although in each case this was restricted to one of the two populations screened. None of the loci were linked. Eight to 10 loci PCR-amplified in three related species: Brown, American White and Pink-backed Pelicans. These loci should prove to be widely applicable to studies of phylogeography and demography in pelicans globally.