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A T Lebedev - One of the best experts on this subject based on the ideXlab platform.

  • differentiation of frogs from two populations belonging to the Pelophylax esculentus complex by lc ms ms comparison of their skin peptidomes
    Analytical and Bioanalytical Chemistry, 2017
    Co-Authors: Tatiana Yu Samgina, Konstantin A Artemenko, Gregor Torkar, Miriam D Tolpina, Polonca Trebše, Jonas Bergquist, A T Lebedev
    Abstract:

    LC-MS/MS was applied to establish the composition of the skin peptidome of a Slovenian green frog belonging to the Pelophylax esculentus complex. As this was similar to the peptidome of the Moscow population of Pelophylax ridibundus, it allowed us to identify the Slovenian frog from the Pelophylax esculentus complex as Pelophylax ridibundus. The sequences of six new peptides from the brevinin 2 family are reported for the first time on the basis of manual interpretation of their tandem mass spectra. The structural similarity of the brevinin 2 peptides from the Moscow and Slovenian populations of Pelophylax ridibundus enables peptides from this family to be utilized as biomarkers for Pelophylax ridibundus inter- and intraspecies differentiation, and the proposed approach can be used as an analytical tool for differentiating the corresponding species and populations. The potential biological activities of the novel peptides were estimated by 2D mass mapping. The results allowed us to classify all of the available peptides belonging to the brevinin 2 family.

  • Differentiation of frogs from two populations belonging to the Pelophylax esculentus complex by LC-MS/MS comparison of their skin peptidomes
    Analytical and Bioanalytical Chemistry, 2017
    Co-Authors: Tatiana Yu Samgina, Konstantin A Artemenko, Gregor Torkar, Miriam D Tolpina, Polonca Trebše, Jonas Bergquist, A T Lebedev
    Abstract:

    LC-MS/MS was applied to establish the composition of the skin peptidome of a Slovenian green frog belonging to the Pelophylax esculentus complex. As this was similar to the peptidome of the Moscow population of Pelophylax ridibundus , it allowed us to identify the Slovenian frog from the Pelophylax esculentus complex as Pelophylax ridibundus . The sequences of six new peptides from the brevinin 2 family are reported for the first time on the basis of manual interpretation of their tandem mass spectra. The structural similarity of the brevinin 2 peptides from the Moscow and Slovenian populations of Pelophylax ridibundus enables peptides from this family to be utilized as biomarkers for Pelophylax ridibundus inter- and intraspecies differentiation, and the proposed approach can be used as an analytical tool for differentiating the corresponding species and populations. The potential biological activities of the novel peptides were estimated by 2D mass mapping. The results allowed us to classify all of the available peptides belonging to the brevinin 2 family. Graphical Abstract Intraspecies identification within the green frog complex

Tatiana Yu Samgina - One of the best experts on this subject based on the ideXlab platform.

  • differentiation of frogs from two populations belonging to the Pelophylax esculentus complex by lc ms ms comparison of their skin peptidomes
    Analytical and Bioanalytical Chemistry, 2017
    Co-Authors: Tatiana Yu Samgina, Konstantin A Artemenko, Gregor Torkar, Miriam D Tolpina, Polonca Trebše, Jonas Bergquist, A T Lebedev
    Abstract:

    LC-MS/MS was applied to establish the composition of the skin peptidome of a Slovenian green frog belonging to the Pelophylax esculentus complex. As this was similar to the peptidome of the Moscow population of Pelophylax ridibundus, it allowed us to identify the Slovenian frog from the Pelophylax esculentus complex as Pelophylax ridibundus. The sequences of six new peptides from the brevinin 2 family are reported for the first time on the basis of manual interpretation of their tandem mass spectra. The structural similarity of the brevinin 2 peptides from the Moscow and Slovenian populations of Pelophylax ridibundus enables peptides from this family to be utilized as biomarkers for Pelophylax ridibundus inter- and intraspecies differentiation, and the proposed approach can be used as an analytical tool for differentiating the corresponding species and populations. The potential biological activities of the novel peptides were estimated by 2D mass mapping. The results allowed us to classify all of the available peptides belonging to the brevinin 2 family.

  • Differentiation of frogs from two populations belonging to the Pelophylax esculentus complex by LC-MS/MS comparison of their skin peptidomes
    Analytical and Bioanalytical Chemistry, 2017
    Co-Authors: Tatiana Yu Samgina, Konstantin A Artemenko, Gregor Torkar, Miriam D Tolpina, Polonca Trebše, Jonas Bergquist, A T Lebedev
    Abstract:

    LC-MS/MS was applied to establish the composition of the skin peptidome of a Slovenian green frog belonging to the Pelophylax esculentus complex. As this was similar to the peptidome of the Moscow population of Pelophylax ridibundus , it allowed us to identify the Slovenian frog from the Pelophylax esculentus complex as Pelophylax ridibundus . The sequences of six new peptides from the brevinin 2 family are reported for the first time on the basis of manual interpretation of their tandem mass spectra. The structural similarity of the brevinin 2 peptides from the Moscow and Slovenian populations of Pelophylax ridibundus enables peptides from this family to be utilized as biomarkers for Pelophylax ridibundus inter- and intraspecies differentiation, and the proposed approach can be used as an analytical tool for differentiating the corresponding species and populations. The potential biological activities of the novel peptides were estimated by 2D mass mapping. The results allowed us to classify all of the available peptides belonging to the brevinin 2 family. Graphical Abstract Intraspecies identification within the green frog complex

O B Stoliar - One of the best experts on this subject based on the ideXlab platform.

  • vulnerability of marsh frog Pelophylax ridibundus to the typical wastewater effluents ibuprofen triclosan and estrone detected by multi biomarker approach
    Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2017
    Co-Authors: Halina Falfushynska, Lesya L Gnatyshyna, Oksana Horyn, O B Stoliar
    Abstract:

    Abstract Pharmaceutical and personal care products (PPCPs) are the environmental pollutants of growing concern. The aim of this study was to indicate the effects of typical PPCPs on the marsh frog Pelophylax ridibundus. We treated male frogs with waterborne ibuprofen (IBU, 250 ng·L− 1), triclosan (TCS, 500 ng·L− 1), or estrone (E1, 100 ng·L− 1) for 14 days. Common vulnerability of the frogs was detected from dramatic decrease of Zn, total and metalated metallothionein (MT) concentrations, Zn/Cu ratio, the elevation of activity of glutathione-S-transferase, cathepsin D and DNA instability in the liver, the depletion of cholinesterase in the brain and cortisol in the blood plasma in all exposures. Nevertheless, lipofuscin concentration in the liver was always decreased. The groups were best distinguished by cytochrome P450 (CYP450) activity determined by ELISA. The exposure to IBU caused lesser damage, but elevated the levels of oxyradicals and glutathione (GSH and GSSG) and lysosomal membrane instability. Exposures to TCS and E1 provoked the endocrine disturbance (increased levels of vitellogenin and thyrotropin in blood plasma), decreased lactate dehydrogenase activity and increased level of pyruvate in the liver. TCS caused the increase of GSSG by 7.3 times and lactate levels. Only E1 lead to decrease of deiodinase activity in the liver, activation of CYP450 and caspase-3 and efflux of cathepsin D from lysosomes. Spectrophotometric and ELISA assays of MTs and CYP450 gave distinct results in E1-group. Broad disruption of the hormonal pathways caused by E1 could be of concern for the health status of frogs in their habitats.

  • a calcium channel blocker nifedipine distorts the effects of nano zinc oxide on metal metabolism in the marsh frog Pelophylax ridibundus
    Saudi Journal of Biological Sciences, 2017
    Co-Authors: Halina Falfushynska, Lesya L Gnatyshyna, Oksana Horyn, Arkadii Shulgai, O B Stoliar
    Abstract:

    Abstract Global decline of amphibian populations causes particular concern about their vulnerability to novel environmental pollutants, including engineering nanomaterials and pharmaceutical products. We evaluated the bioavailability of nanoform of zinc oxide (n-ZnO) in frog Pelophylax ridibundus and determined whether co-exposure to a common pharmaceutical, a calcium-channel blocker nifedipine (Nfd) can affect this bioavailability. Male frogs were exposed for 14 days to the tap water (Control) and n-ZnO (3.1 μM), Zn2+ (3.1 μM, as a positive control for n-ZnO exposures), Nfd (10 μM), and combination of n-ZnO and Nfd (n-ZnO + Nfd) in environmentally-relevant concentration. Exposure to Zn2+ or n-ZnO led to up-regulation of metal-binding proteins, metallothioneins (MTs) in the liver and Zn-carrying vitellogenin-like proteins in the blood plasma. Notably, upregulation of MTs by Zn2+ or n-ZnO exposures combined with increased binding of Zn and Cu to MTs. This was associated with the more reducing conditions in the liver tissue indicated by elevated lactate to pyruvate ratio. Nfd suppressed the binding of Zn and Cu to MTs and led to a decrease in Lactate/Pyruvate ratio and elevated protein carbonylation indicating pro-oxidant conditions. Redox status parameters were not directly related to DNA fragmentation, nuclear abnormalities or suppression of cholinesterase activity indicating that factors other than oxidative stress are involved in cytotoxicity of different pollutants and their combinations. Furthermore, activity of Phase I biotransformation enzyme (CYP450 oxidase measured as EROD) was elevated in Nfd-containing exposures and in Zn2+ exposed frogs. Tyrosinase-like activity in the frog liver was strongly stimulated by Zn2+ but suppressed by n-ZnO, Nfd and n-ZnO + Nfd. These findings show that Nfd modulates homeostasis of essential metals in amphibians and emphasize that physiological consequences of combined n-ZnO and Nfd exposures are difficult to predict based on the mechanisms of single stressors.

  • endocrine and cellular stress effects of zinc oxide nanoparticles and nifedipine in marsh frogs Pelophylax ridibundus
    Aquatic Toxicology, 2017
    Co-Authors: Halina Falfushynska, Lesya L Gnatyshyna, Oksana Horyn, Inna M Sokolova, O B Stoliar
    Abstract:

    Abstract Freshwater organisms including amphibians experience increasing exposures to emerging pollutants such as nanoparticles and pharmaceuticals, which can affect their fitness and performance. We studied the effects of two common pollutants extensively used in industry, pharmaceutical and personal care products, nano-zinc oxide (nZnO) and a Ca-channel blocker nifedipine (Nfd), on endocrine status and cellular stress markers of the marsh frog Pelophylax ridibundus . Males were exposed for 14 days to nZnO (3.1 μM), Zn 2+ (3.1 μM, as a positive control for nZnO exposures), Nfd (10 μM), and combination of nZnO and Nfd (nZnO + Nfd). Exposure to nZnO and Zn 2+ led to an increase in Zn burdens, elevated concentrations of the metal-bound metallothioneins (MT-Me) in the liver and increased vitellogenin in the serum, whereas exposures to Nfd and nZnO + Nfd resulted in the metal release from MTs and a significant increase in the ratio of total to metal-bound MTs. This likely reflects oxidative stress caused by Nfd exposures as manifested in the elevated levels of oxyradical production, upregulation of superoxide dismutase activity (SOD) and increase in the total and oxidized glutathione concentrations in Nfd-exposed frogs. Zn-containing exposures upregulated activity of deiodinase (in nZnO and nZnO + Nfd exposures) and serum thyrotropin level (in the case of Zn 2+ ). All exposures caused an increase in DNA fragmentation, lipofuscin accumulation as well as upregulation of caspase-3 and CYP450 levels reflecting cytotoxicity of the studied compounds in the liver. Across all experimental treatments, nZnO exposures in the absence of Nfd had the least impact on the cellular stress traits or redox status in frogs. This indicates that at the low environmentally relevant levels of pollution, pharmaceuticals such as Nfd and free metals (such as Zn 2+ ) may represent a stronger threat to the health of the frogs than nZnO particles.

  • endocrine activities and cellular stress responses in the marsh frog Pelophylax ridibundus exposed to cobalt zinc and their organic nanocomplexes
    Aquatic Toxicology, 2016
    Co-Authors: Halina Falfushynska, Lesya L Gnatyshyna, Inna M Sokolova, Olga Fedoruk, O B Stoliar
    Abstract:

    Abstract Metal-containing materials are extensively used in industry, personal care products and medicine, and their release in the environment causes concern for the potential impacts on aquatic organisms. We assessed endocrine disrupting potential of N -vinyl-2-pyrrolidone-based nanoparticles (Me-PSs) containing cobalt (Co 2+ ) or zinc (Zn 2+ ), using the marsh frog Pelophylax ridibundus as a model. Adult males were exposed for 14 days to waterborne Co 2+ (50 μg/L), Zn 2+ (100 μg/L) or corresponding concentrations of Co-PS, Zn-PS, or parental polymeric compound (PS). The indices of thyroid activity, vitellogenesis, cytochrome P450-dependent monooxygenases activity (EROD) and cytotoxicity markers were evaluated. Exposure to Co 2+ led to the elevation of serum thyrotropin (TSH) and hepatic deiodinase activities accompanied by the up-regulation of EROD activity. In contrast, the action of the polymer-containing substances (Co-PS, Zn-PS and PS) as well as free Zn 2+ caused a prominent decrease of EROD activity and a decrease in serum cortisol and TSH concentrations. Exposures to Zn 2+ , Zn-PS and PS upregulated vitellogenesis in males. All exposures except Co 2+ caused neurotoxicity as indicated by the depletion of cholinesterase. These results demonstrate toxicity of Co- and Zn-containing Me-PSs and their parental compounds (Zn 2+ and PS) in frogs and indicate distinct mechanisms of Co 2+ action. Broad disruption of the hormonal pathways and reduced capacity for organic xenobiotic detoxification may have deleterious impacts on amphibian populations from habitats exposed to metallorganic pollution.

Halina Falfushynska - One of the best experts on this subject based on the ideXlab platform.

  • vulnerability of marsh frog Pelophylax ridibundus to the typical wastewater effluents ibuprofen triclosan and estrone detected by multi biomarker approach
    Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2017
    Co-Authors: Halina Falfushynska, Lesya L Gnatyshyna, Oksana Horyn, O B Stoliar
    Abstract:

    Abstract Pharmaceutical and personal care products (PPCPs) are the environmental pollutants of growing concern. The aim of this study was to indicate the effects of typical PPCPs on the marsh frog Pelophylax ridibundus. We treated male frogs with waterborne ibuprofen (IBU, 250 ng·L− 1), triclosan (TCS, 500 ng·L− 1), or estrone (E1, 100 ng·L− 1) for 14 days. Common vulnerability of the frogs was detected from dramatic decrease of Zn, total and metalated metallothionein (MT) concentrations, Zn/Cu ratio, the elevation of activity of glutathione-S-transferase, cathepsin D and DNA instability in the liver, the depletion of cholinesterase in the brain and cortisol in the blood plasma in all exposures. Nevertheless, lipofuscin concentration in the liver was always decreased. The groups were best distinguished by cytochrome P450 (CYP450) activity determined by ELISA. The exposure to IBU caused lesser damage, but elevated the levels of oxyradicals and glutathione (GSH and GSSG) and lysosomal membrane instability. Exposures to TCS and E1 provoked the endocrine disturbance (increased levels of vitellogenin and thyrotropin in blood plasma), decreased lactate dehydrogenase activity and increased level of pyruvate in the liver. TCS caused the increase of GSSG by 7.3 times and lactate levels. Only E1 lead to decrease of deiodinase activity in the liver, activation of CYP450 and caspase-3 and efflux of cathepsin D from lysosomes. Spectrophotometric and ELISA assays of MTs and CYP450 gave distinct results in E1-group. Broad disruption of the hormonal pathways caused by E1 could be of concern for the health status of frogs in their habitats.

  • a calcium channel blocker nifedipine distorts the effects of nano zinc oxide on metal metabolism in the marsh frog Pelophylax ridibundus
    Saudi Journal of Biological Sciences, 2017
    Co-Authors: Halina Falfushynska, Lesya L Gnatyshyna, Oksana Horyn, Arkadii Shulgai, O B Stoliar
    Abstract:

    Abstract Global decline of amphibian populations causes particular concern about their vulnerability to novel environmental pollutants, including engineering nanomaterials and pharmaceutical products. We evaluated the bioavailability of nanoform of zinc oxide (n-ZnO) in frog Pelophylax ridibundus and determined whether co-exposure to a common pharmaceutical, a calcium-channel blocker nifedipine (Nfd) can affect this bioavailability. Male frogs were exposed for 14 days to the tap water (Control) and n-ZnO (3.1 μM), Zn2+ (3.1 μM, as a positive control for n-ZnO exposures), Nfd (10 μM), and combination of n-ZnO and Nfd (n-ZnO + Nfd) in environmentally-relevant concentration. Exposure to Zn2+ or n-ZnO led to up-regulation of metal-binding proteins, metallothioneins (MTs) in the liver and Zn-carrying vitellogenin-like proteins in the blood plasma. Notably, upregulation of MTs by Zn2+ or n-ZnO exposures combined with increased binding of Zn and Cu to MTs. This was associated with the more reducing conditions in the liver tissue indicated by elevated lactate to pyruvate ratio. Nfd suppressed the binding of Zn and Cu to MTs and led to a decrease in Lactate/Pyruvate ratio and elevated protein carbonylation indicating pro-oxidant conditions. Redox status parameters were not directly related to DNA fragmentation, nuclear abnormalities or suppression of cholinesterase activity indicating that factors other than oxidative stress are involved in cytotoxicity of different pollutants and their combinations. Furthermore, activity of Phase I biotransformation enzyme (CYP450 oxidase measured as EROD) was elevated in Nfd-containing exposures and in Zn2+ exposed frogs. Tyrosinase-like activity in the frog liver was strongly stimulated by Zn2+ but suppressed by n-ZnO, Nfd and n-ZnO + Nfd. These findings show that Nfd modulates homeostasis of essential metals in amphibians and emphasize that physiological consequences of combined n-ZnO and Nfd exposures are difficult to predict based on the mechanisms of single stressors.

  • endocrine and cellular stress effects of zinc oxide nanoparticles and nifedipine in marsh frogs Pelophylax ridibundus
    Aquatic Toxicology, 2017
    Co-Authors: Halina Falfushynska, Lesya L Gnatyshyna, Oksana Horyn, Inna M Sokolova, O B Stoliar
    Abstract:

    Abstract Freshwater organisms including amphibians experience increasing exposures to emerging pollutants such as nanoparticles and pharmaceuticals, which can affect their fitness and performance. We studied the effects of two common pollutants extensively used in industry, pharmaceutical and personal care products, nano-zinc oxide (nZnO) and a Ca-channel blocker nifedipine (Nfd), on endocrine status and cellular stress markers of the marsh frog Pelophylax ridibundus . Males were exposed for 14 days to nZnO (3.1 μM), Zn 2+ (3.1 μM, as a positive control for nZnO exposures), Nfd (10 μM), and combination of nZnO and Nfd (nZnO + Nfd). Exposure to nZnO and Zn 2+ led to an increase in Zn burdens, elevated concentrations of the metal-bound metallothioneins (MT-Me) in the liver and increased vitellogenin in the serum, whereas exposures to Nfd and nZnO + Nfd resulted in the metal release from MTs and a significant increase in the ratio of total to metal-bound MTs. This likely reflects oxidative stress caused by Nfd exposures as manifested in the elevated levels of oxyradical production, upregulation of superoxide dismutase activity (SOD) and increase in the total and oxidized glutathione concentrations in Nfd-exposed frogs. Zn-containing exposures upregulated activity of deiodinase (in nZnO and nZnO + Nfd exposures) and serum thyrotropin level (in the case of Zn 2+ ). All exposures caused an increase in DNA fragmentation, lipofuscin accumulation as well as upregulation of caspase-3 and CYP450 levels reflecting cytotoxicity of the studied compounds in the liver. Across all experimental treatments, nZnO exposures in the absence of Nfd had the least impact on the cellular stress traits or redox status in frogs. This indicates that at the low environmentally relevant levels of pollution, pharmaceuticals such as Nfd and free metals (such as Zn 2+ ) may represent a stronger threat to the health of the frogs than nZnO particles.

  • endocrine activities and cellular stress responses in the marsh frog Pelophylax ridibundus exposed to cobalt zinc and their organic nanocomplexes
    Aquatic Toxicology, 2016
    Co-Authors: Halina Falfushynska, Lesya L Gnatyshyna, Inna M Sokolova, Olga Fedoruk, O B Stoliar
    Abstract:

    Abstract Metal-containing materials are extensively used in industry, personal care products and medicine, and their release in the environment causes concern for the potential impacts on aquatic organisms. We assessed endocrine disrupting potential of N -vinyl-2-pyrrolidone-based nanoparticles (Me-PSs) containing cobalt (Co 2+ ) or zinc (Zn 2+ ), using the marsh frog Pelophylax ridibundus as a model. Adult males were exposed for 14 days to waterborne Co 2+ (50 μg/L), Zn 2+ (100 μg/L) or corresponding concentrations of Co-PS, Zn-PS, or parental polymeric compound (PS). The indices of thyroid activity, vitellogenesis, cytochrome P450-dependent monooxygenases activity (EROD) and cytotoxicity markers were evaluated. Exposure to Co 2+ led to the elevation of serum thyrotropin (TSH) and hepatic deiodinase activities accompanied by the up-regulation of EROD activity. In contrast, the action of the polymer-containing substances (Co-PS, Zn-PS and PS) as well as free Zn 2+ caused a prominent decrease of EROD activity and a decrease in serum cortisol and TSH concentrations. Exposures to Zn 2+ , Zn-PS and PS upregulated vitellogenesis in males. All exposures except Co 2+ caused neurotoxicity as indicated by the depletion of cholinesterase. These results demonstrate toxicity of Co- and Zn-containing Me-PSs and their parental compounds (Zn 2+ and PS) in frogs and indicate distinct mechanisms of Co 2+ action. Broad disruption of the hormonal pathways and reduced capacity for organic xenobiotic detoxification may have deleterious impacts on amphibian populations from habitats exposed to metallorganic pollution.

Gregor Torkar - One of the best experts on this subject based on the ideXlab platform.

  • differentiation of frogs from two populations belonging to the Pelophylax esculentus complex by lc ms ms comparison of their skin peptidomes
    Analytical and Bioanalytical Chemistry, 2017
    Co-Authors: Tatiana Yu Samgina, Konstantin A Artemenko, Gregor Torkar, Miriam D Tolpina, Polonca Trebše, Jonas Bergquist, A T Lebedev
    Abstract:

    LC-MS/MS was applied to establish the composition of the skin peptidome of a Slovenian green frog belonging to the Pelophylax esculentus complex. As this was similar to the peptidome of the Moscow population of Pelophylax ridibundus, it allowed us to identify the Slovenian frog from the Pelophylax esculentus complex as Pelophylax ridibundus. The sequences of six new peptides from the brevinin 2 family are reported for the first time on the basis of manual interpretation of their tandem mass spectra. The structural similarity of the brevinin 2 peptides from the Moscow and Slovenian populations of Pelophylax ridibundus enables peptides from this family to be utilized as biomarkers for Pelophylax ridibundus inter- and intraspecies differentiation, and the proposed approach can be used as an analytical tool for differentiating the corresponding species and populations. The potential biological activities of the novel peptides were estimated by 2D mass mapping. The results allowed us to classify all of the available peptides belonging to the brevinin 2 family.

  • Differentiation of frogs from two populations belonging to the Pelophylax esculentus complex by LC-MS/MS comparison of their skin peptidomes
    Analytical and Bioanalytical Chemistry, 2017
    Co-Authors: Tatiana Yu Samgina, Konstantin A Artemenko, Gregor Torkar, Miriam D Tolpina, Polonca Trebše, Jonas Bergquist, A T Lebedev
    Abstract:

    LC-MS/MS was applied to establish the composition of the skin peptidome of a Slovenian green frog belonging to the Pelophylax esculentus complex. As this was similar to the peptidome of the Moscow population of Pelophylax ridibundus , it allowed us to identify the Slovenian frog from the Pelophylax esculentus complex as Pelophylax ridibundus . The sequences of six new peptides from the brevinin 2 family are reported for the first time on the basis of manual interpretation of their tandem mass spectra. The structural similarity of the brevinin 2 peptides from the Moscow and Slovenian populations of Pelophylax ridibundus enables peptides from this family to be utilized as biomarkers for Pelophylax ridibundus inter- and intraspecies differentiation, and the proposed approach can be used as an analytical tool for differentiating the corresponding species and populations. The potential biological activities of the novel peptides were estimated by 2D mass mapping. The results allowed us to classify all of the available peptides belonging to the brevinin 2 family. Graphical Abstract Intraspecies identification within the green frog complex