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T A Lovick - One of the best experts on this subject based on the ideXlab platform.

  • suppression of urinary voiding by conditional high frequency stimulation of the Pelvic Nerve in conscious rats
    Frontiers in Physiology, 2018
    Co-Authors: Charly B. J. Brouillard, Jonathan J. Crook, Pedro P. Irazoqui, T A Lovick
    Abstract:

    Female Wistar rats were instrumented to record bladder pressure and to stimulate the left Pelvic Nerve. Repeated voids were induced by continuous infusion of saline into the bladder (11.2 ml/h) via a T-piece in the line to the bladder catheter. In each animal tested (n = 6) high frequency Pelvic Nerve stimulation (1-3 kHz, 1-2 mA sinusoidal waveform for 60 s) applied within 2 s of the onset of a sharp rise in bladder pressure signaling an imminent void was able to inhibit micturition. Voiding was modulated in three ways: (1) Suppression of voiding (four rats, n = 13 trials). No fluid output or a very small volume of fluid expelled (<15% of the volume expected based on the mean of the previous 2 or 3 voids). Voiding suppressed for the entirety of the stimulation period (60 s) and resumed within 37 s of stopping stimulation. (2) Void deferred (four rats, n = 6 trials). The imminent void was suppressed (no fluid expelled) but a void occurred later in the stimulation period (12-44 s, mean 24.5 ± 5.2 s after the onset of the stimulation). (3) Reduction in voided volume (five rats, n = 20 trials). Voiding took place but the volume of fluid voided was 15-80% (range 21.8-77.8%, mean 45.3 ± 3.6%) of the volume expected from the mean of the preceding two or three voids. Spontaneous voiding resumed within 5 min of stopping stimulation. Stimulation during the filling phase in between voids had no effect. The experiments demonstrate that conditional high frequency stimulation of the Pelvic Nerve started at the onset of an imminent void can inhibit voiding. The effect was rapidly reversible and was not accompanied by any adverse behavioral side effects.

  • chronic implantation of cuff electrodes on the Pelvic Nerve in rats is well tolerated and does not compromise afferent or efferent fibre functionality
    Journal of Neural Engineering, 2018
    Co-Authors: Jon Crook, Charly B. J. Brouillard, Pedro P. Irazoqui, T A Lovick
    Abstract:

    Objective. Neuromodulation of autonomic Nerve activity to regulate physiological processes is an emerging field. Vagal stimulation has received most attention whereas the potential of modulate visceral function by targeting autonomic Nerves within the abdominal cavity remains under-exploited. Surgery to locate intra-abdominal targets is inherently more stressful than for peripheral Nerves. Electrode leads risk becoming entrapped by intestines and loss of functionality in the Nerve-target organ connection could result from electrode migration or twisting. Since nociceptor afferents are intermingled with similar-sized visceral autonomic fibres, stimulation may induce pain. In anaesthetised rats high frequency stimulation of the Pelvic Nerve can suppress urinary voiding but it is not known how conscious animals would react to this procedure. Our objective therefore was to determine how rats tolerated chronic implantation of cuff electrodes on the Pelvic Nerve, whether Nerve stimulation would be aversive and whether Nerve-bladder functionality would be compromised. Approach. We carried out a preliminary de-risking study to investigate how conscious rats tolerated chronic implantation of electrodes on the Pelvic Nerve, their responsiveness to intermittent high frequency stimulation and whether functionality of the Nerve-bladder connection became compromised. Main results. Implantation of cuff electrodes was well-tolerated. The normal diurnal pattern of urinary voiding was not disrupted. Pelvic Nerve stimulation (up to 4 mA, 3 kHz) for 30 min periods evoked mild alerting at stimulus onset but no signs of pain. Stimulation evoked a modest (<0.5 °C) increase in Nerve temperature but the functional integrity of the Nerve-bladder connection, reflected by contraction of the detrusor muscle in response to 10 Hz Nerve stimulation, was not compromised. Significance. Chronic implantation of cuff electrodes on the Pelvic Nerve was found to be a well-tolerated procedure in rats and high frequency stimulation did not lead to loss of Nerve functionality. Pelvic Nerve stimulation has development potential for normalizing voiding dysfunction in conscious rats.

Tzer Bin Lin - One of the best experts on this subject based on the ideXlab platform.

Thelma A. Lovick - One of the best experts on this subject based on the ideXlab platform.

  • Suppression of Urinary Voiding by Conditional High Frequency Stimulation of the Pelvic Nerve in Conscious Rats.
    Frontiers in physiology, 2018
    Co-Authors: Charly B. J. Brouillard, Jonathan J. Crook, Pedro P. Irazoqui, Thelma A. Lovick
    Abstract:

    Female Wistar rats were instrumented to record bladder pressure and to stimulate the left Pelvic Nerve. Repeated voids were induced by continuous infusion of saline into the bladder (11.2ml/h) via a T-piece in the line to the bladder catheter. In each animal tested (n=6) high frequency Pelvic Nerve stimulation (1-3kHz, 1-2mA sinusoidal waveform for 60s) applied within 2s of the onset of a sharp rise in bladder pressure signaling an imminent void was able to inhibit micturition. Voiding was modulated in three ways: 1. Suppression of voiding (4 rats, n=13 trials). No fluid output or a very small volume of fluid expelled (

  • Chronic implantation of cuff electrodes on the Pelvic Nerve in rats is well tolerated and does not compromise afferent or efferent fibre functionality.
    Journal of neural engineering, 2018
    Co-Authors: Jon Crook, Pedro P. Irazoqui, Charly B. J. Brouillard, Thelma A. Lovick
    Abstract:

    Objective. Neuromodulation of autonomic Nerve activity to regulate physiological processes is an emerging field. Vagal stimulation has received most attention whereas the potential of modulate visceral function by targeting autonomic Nerves within the abdominal cavity remains under-exploited. Surgery to locate intra-abdominal targets is inherently more stressful than for peripheral Nerves. Electrode leads risk becoming entrapped by intestines and loss of functionality in the Nerve-target organ connection could result from electrode migration or twisting. Since nociceptor afferents are intermingled with similar-sized visceral autonomic fibres, stimulation may induce pain. In anaesthetised rats high frequency stimulation of the Pelvic Nerve can suppress urinary voiding but it is not known how conscious animals would react to this procedure. Our objective therefore was to determine how rats tolerated chronic implantation of cuff electrodes on the Pelvic Nerve, whether Nerve stimulation would be aversive and whether Nerve-bladder functionality would be compromised. Approach. We carried out a preliminary de-risking study to investigate how conscious rats tolerated chronic implantation of electrodes on the Pelvic Nerve, their responsiveness to intermittent high frequency stimulation and whether functionality of the Nerve-bladder connection became compromised. Main results. Implantation of cuff electrodes was well-tolerated. The normal diurnal pattern of urinary voiding was not disrupted. Pelvic Nerve stimulation (up to 4 mA, 3 kHz) for 30 min periods evoked mild alerting at stimulus onset but no signs of pain. Stimulation evoked a modest (

  • high frequency stimulation of the Pelvic Nerve inhibits urinary voiding in anesthetized rats
    Frontiers in Physiology, 2017
    Co-Authors: Jonathan J. Crook, Thelma A. Lovick
    Abstract:

    Urge Urinary Incontinence: ‘a sudden and uncontrollable desire to void which is impossible to defer’ is extremely common and considered the most bothersome of lower urinary tract conditions. Current treatments rely on pharmacological, neuromodulatory and neurotoxicological approaches to manage the disorder, by reducing the excitability of the bladder muscle. However, some patients remain refractory to treatment. An alternative approach would be to temporarily suppress activity of the micturition control circuitry at the time of need i.e. urgency. In this study we investigated, in a rat model, the utility of high frequency Pelvic Nerve stimulation to produce a rapid onset, reversible suppression of voiding. In urethane-anaesthetised rats periodic voiding was induced by continuous infusion of saline into the bladder whilst recording bladder pressure and electrical activity from the external urethral sphincter. High frequency (1-3kHz), sinusoidal Pelvic Nerve stimulation initiated at the onset of the sharp rise in bladder pressure signalling an imminent void aborted the detrusor contraction. Urine output was suppressed and tone in the external urethral sphincter increased. Stimulating the right or left Nerve was equally effective). The effect was rapid in onset, reversible and reproducible and evoked only minimal ‘off target’ side effects on blood pressure, heart rate, respiration, uterine pressure or rectal pressure. Transient contraction of abdominal wall was observed in some animals. Stimulation applied during the filling phase evoked a small, transient rise in bladder pressure and increased tonic activity in the EUS, but no urine output. Suppression of micturition persisted after section of the contralateral Pelvic Nerve or after ligation of the Nerve distal to the electrode cuff on the ipsilateral side. We conclude that high frequency Pelvic Nerve stimulation initiated at the onset of an imminent void provides a potential means to control urinary continence.

  • High Frequency Stimulation of the Pelvic Nerve Inhibits Urinary Voiding in Anesthetized Rats
    Frontiers Media S.A., 2017
    Co-Authors: Jonathan J. Crook, Thelma A. Lovick
    Abstract:

    Urge Urinary Incontinence: “a sudden and uncontrollable desire to void which is impossible to defer” is extremely common and considered the most bothersome of lower urinary tract conditions. Current treatments rely on pharmacological, neuromodulatory, and neurotoxicological approaches to manage the disorder, by reducing the excitability of the bladder muscle. However, some patients remain refractory to treatment. An alternative approach would be to temporarily suppress activity of the micturition control circuitry at the time of need i.e., urgency. In this study we investigated, in a rat model, the utility of high frequency Pelvic Nerve stimulation to produce a rapid onset, reversible suppression of voiding. In urethane-anesthetized rats periodic voiding was induced by continuous infusion of saline into the bladder whilst recording bladder pressure and electrical activity from the external urethral sphincter (EUS). High frequency (1–3 kHz), sinusoidal Pelvic Nerve stimulation initiated at the onset of the sharp rise in bladder pressure signaling an imminent void aborted the detrusor contraction. Urine output was suppressed and tone in the EUS increased. Stimulating the right or left Nerve was equally effective. The effect was rapid in onset, reversible, and reproducible and evoked only minimal “off target” side effects on blood pressure, heart rate, respiration, uterine pressure, or rectal pressure. Transient contraction of abdominal wall was observed in some animals. Stimulation applied during the filling phase evoked a small, transient rise in bladder pressure and increased tonic activity in the EUS, but no urine output. Suppression of micturition persisted after section of the contralateral Pelvic Nerve or after ligation of the Nerve distal to the electrode cuff on the ipsilateral side. We conclude that high frequency Pelvic Nerve stimulation initiated at the onset of an imminent void provides a potential means to control urinary continence

Jiuan Miaw Liao - One of the best experts on this subject based on the ideXlab platform.

Charly B. J. Brouillard - One of the best experts on this subject based on the ideXlab platform.

  • Suppression of Urinary Voiding by Conditional High Frequency Stimulation of the Pelvic Nerve in Conscious Rats.
    Frontiers in physiology, 2018
    Co-Authors: Charly B. J. Brouillard, Jonathan J. Crook, Pedro P. Irazoqui, Thelma A. Lovick
    Abstract:

    Female Wistar rats were instrumented to record bladder pressure and to stimulate the left Pelvic Nerve. Repeated voids were induced by continuous infusion of saline into the bladder (11.2ml/h) via a T-piece in the line to the bladder catheter. In each animal tested (n=6) high frequency Pelvic Nerve stimulation (1-3kHz, 1-2mA sinusoidal waveform for 60s) applied within 2s of the onset of a sharp rise in bladder pressure signaling an imminent void was able to inhibit micturition. Voiding was modulated in three ways: 1. Suppression of voiding (4 rats, n=13 trials). No fluid output or a very small volume of fluid expelled (

  • suppression of urinary voiding by conditional high frequency stimulation of the Pelvic Nerve in conscious rats
    Frontiers in Physiology, 2018
    Co-Authors: Charly B. J. Brouillard, Jonathan J. Crook, Pedro P. Irazoqui, T A Lovick
    Abstract:

    Female Wistar rats were instrumented to record bladder pressure and to stimulate the left Pelvic Nerve. Repeated voids were induced by continuous infusion of saline into the bladder (11.2 ml/h) via a T-piece in the line to the bladder catheter. In each animal tested (n = 6) high frequency Pelvic Nerve stimulation (1-3 kHz, 1-2 mA sinusoidal waveform for 60 s) applied within 2 s of the onset of a sharp rise in bladder pressure signaling an imminent void was able to inhibit micturition. Voiding was modulated in three ways: (1) Suppression of voiding (four rats, n = 13 trials). No fluid output or a very small volume of fluid expelled (<15% of the volume expected based on the mean of the previous 2 or 3 voids). Voiding suppressed for the entirety of the stimulation period (60 s) and resumed within 37 s of stopping stimulation. (2) Void deferred (four rats, n = 6 trials). The imminent void was suppressed (no fluid expelled) but a void occurred later in the stimulation period (12-44 s, mean 24.5 ± 5.2 s after the onset of the stimulation). (3) Reduction in voided volume (five rats, n = 20 trials). Voiding took place but the volume of fluid voided was 15-80% (range 21.8-77.8%, mean 45.3 ± 3.6%) of the volume expected from the mean of the preceding two or three voids. Spontaneous voiding resumed within 5 min of stopping stimulation. Stimulation during the filling phase in between voids had no effect. The experiments demonstrate that conditional high frequency stimulation of the Pelvic Nerve started at the onset of an imminent void can inhibit voiding. The effect was rapidly reversible and was not accompanied by any adverse behavioral side effects.

  • chronic implantation of cuff electrodes on the Pelvic Nerve in rats is well tolerated and does not compromise afferent or efferent fibre functionality
    Journal of Neural Engineering, 2018
    Co-Authors: Jon Crook, Charly B. J. Brouillard, Pedro P. Irazoqui, T A Lovick
    Abstract:

    Objective. Neuromodulation of autonomic Nerve activity to regulate physiological processes is an emerging field. Vagal stimulation has received most attention whereas the potential of modulate visceral function by targeting autonomic Nerves within the abdominal cavity remains under-exploited. Surgery to locate intra-abdominal targets is inherently more stressful than for peripheral Nerves. Electrode leads risk becoming entrapped by intestines and loss of functionality in the Nerve-target organ connection could result from electrode migration or twisting. Since nociceptor afferents are intermingled with similar-sized visceral autonomic fibres, stimulation may induce pain. In anaesthetised rats high frequency stimulation of the Pelvic Nerve can suppress urinary voiding but it is not known how conscious animals would react to this procedure. Our objective therefore was to determine how rats tolerated chronic implantation of cuff electrodes on the Pelvic Nerve, whether Nerve stimulation would be aversive and whether Nerve-bladder functionality would be compromised. Approach. We carried out a preliminary de-risking study to investigate how conscious rats tolerated chronic implantation of electrodes on the Pelvic Nerve, their responsiveness to intermittent high frequency stimulation and whether functionality of the Nerve-bladder connection became compromised. Main results. Implantation of cuff electrodes was well-tolerated. The normal diurnal pattern of urinary voiding was not disrupted. Pelvic Nerve stimulation (up to 4 mA, 3 kHz) for 30 min periods evoked mild alerting at stimulus onset but no signs of pain. Stimulation evoked a modest (<0.5 °C) increase in Nerve temperature but the functional integrity of the Nerve-bladder connection, reflected by contraction of the detrusor muscle in response to 10 Hz Nerve stimulation, was not compromised. Significance. Chronic implantation of cuff electrodes on the Pelvic Nerve was found to be a well-tolerated procedure in rats and high frequency stimulation did not lead to loss of Nerve functionality. Pelvic Nerve stimulation has development potential for normalizing voiding dysfunction in conscious rats.

  • Chronic implantation of cuff electrodes on the Pelvic Nerve in rats is well tolerated and does not compromise afferent or efferent fibre functionality.
    Journal of neural engineering, 2018
    Co-Authors: Jon Crook, Pedro P. Irazoqui, Charly B. J. Brouillard, Thelma A. Lovick
    Abstract:

    Objective. Neuromodulation of autonomic Nerve activity to regulate physiological processes is an emerging field. Vagal stimulation has received most attention whereas the potential of modulate visceral function by targeting autonomic Nerves within the abdominal cavity remains under-exploited. Surgery to locate intra-abdominal targets is inherently more stressful than for peripheral Nerves. Electrode leads risk becoming entrapped by intestines and loss of functionality in the Nerve-target organ connection could result from electrode migration or twisting. Since nociceptor afferents are intermingled with similar-sized visceral autonomic fibres, stimulation may induce pain. In anaesthetised rats high frequency stimulation of the Pelvic Nerve can suppress urinary voiding but it is not known how conscious animals would react to this procedure. Our objective therefore was to determine how rats tolerated chronic implantation of cuff electrodes on the Pelvic Nerve, whether Nerve stimulation would be aversive and whether Nerve-bladder functionality would be compromised. Approach. We carried out a preliminary de-risking study to investigate how conscious rats tolerated chronic implantation of electrodes on the Pelvic Nerve, their responsiveness to intermittent high frequency stimulation and whether functionality of the Nerve-bladder connection became compromised. Main results. Implantation of cuff electrodes was well-tolerated. The normal diurnal pattern of urinary voiding was not disrupted. Pelvic Nerve stimulation (up to 4 mA, 3 kHz) for 30 min periods evoked mild alerting at stimulus onset but no signs of pain. Stimulation evoked a modest (