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Saengchan Senapin - One of the best experts on this subject based on the ideXlab platform.

  • infections of mrnv macrobrachium rosenbergii nodavirus in cultivated whiteleg shrimp Penaeus vannamei in asia
    Aquaculture, 2012
    Co-Authors: Saengchan Senapin, Chatlada Jaengsanong, Kornsunee Phiwsaiya, Satit Prasertsri, Kesinee Laisutisan, Niti Chuchird, Chalor Limsuwan
    Abstract:

    Abstract Macrobrachium rosenbergii nodavirus ( Mr NV) is well-known as a major pathogen that causes whitened muscles and high mortality in the freshwater prawn Macrobrachium rosenbergii . Recently, it has also been reported to cause white muscles and high mortality in postlarvae of the marine shrimp Penaeus ( Penaeus ) monodon and Penaeus ( FenneroPenaeus ) indicus in India. The latter report stimulated us to re-examine specimens from Asian shrimp farms that had experienced high mortality in Penaeus ( LitoPenaeus ) vannamei with white muscles but tested negative for infectious myonecrosis virus (IMNV) and Penaeus vannamei nodavirus ( Pv NV) by RT-PCR analysis. Some of these specimens were indeed positive for Mr NV by RT-PCR. Sequences of the two single-stranded RNA fragments in the Mr NV genome were amplified from these P. vannamei specimens and found to share ~ 97% nucleic acid sequence identity with the Mr NV sequences deposited at GenBank ( NC_005094 and NC_005095 NC_005094 NC_005095 ). Extra small virus (XSV) usually associated with Mr NV, was detected in some but not all of the samples. Infectivity tests were performed by feeding P. vannamei with minced tissues from Mr NV-infected M. rosenbergii . The assays were preformed at low salinity of 2 ppt and at two different water temperatures of approximately 22 °C and 28 °C. It was revealed that shrimp exhibited a higher infectivity and mortality at the lower temperature. Our findings suggested that P. vannamei is an additional species that is susceptible to Mr NV and that low temperature together with low salinity of rearing water may increase the severity of infections leading to significant mortality.

T Aoki - One of the best experts on this subject based on the ideXlab platform.

  • fusarium incarnatum isolated from black tiger shrimp Penaeus monodon fabricius with black gill disease cultured in vietnam
    Journal of Fish Diseases, 2004
    Co-Authors: L V Khoa, Kishio Hatai, T Aoki
    Abstract:

    : Fusarium incarnatum was isolated from gill lesions of cultured black tiger shrimp, Penaeus monodon, in every crop during 2000-2002 in Nghe An province, Vietnam. Infected shrimps showed typical signs of black gill disease and mortalities about a month prior to harvest. Detailed morphological examinations, as well as molecular phylogenic analyses based on partial nucleotide sequences of ribosomal DNA, were made on the isolates. An artificial infection of kuruma prawn, Penaeus japonicus, using two selected isolates was also conducted and their pathogenicity determined.

Chalor Limsuwan - One of the best experts on this subject based on the ideXlab platform.

  • infections of mrnv macrobrachium rosenbergii nodavirus in cultivated whiteleg shrimp Penaeus vannamei in asia
    Aquaculture, 2012
    Co-Authors: Saengchan Senapin, Chatlada Jaengsanong, Kornsunee Phiwsaiya, Satit Prasertsri, Kesinee Laisutisan, Niti Chuchird, Chalor Limsuwan
    Abstract:

    Abstract Macrobrachium rosenbergii nodavirus ( Mr NV) is well-known as a major pathogen that causes whitened muscles and high mortality in the freshwater prawn Macrobrachium rosenbergii . Recently, it has also been reported to cause white muscles and high mortality in postlarvae of the marine shrimp Penaeus ( Penaeus ) monodon and Penaeus ( FenneroPenaeus ) indicus in India. The latter report stimulated us to re-examine specimens from Asian shrimp farms that had experienced high mortality in Penaeus ( LitoPenaeus ) vannamei with white muscles but tested negative for infectious myonecrosis virus (IMNV) and Penaeus vannamei nodavirus ( Pv NV) by RT-PCR analysis. Some of these specimens were indeed positive for Mr NV by RT-PCR. Sequences of the two single-stranded RNA fragments in the Mr NV genome were amplified from these P. vannamei specimens and found to share ~ 97% nucleic acid sequence identity with the Mr NV sequences deposited at GenBank ( NC_005094 and NC_005095 NC_005094 NC_005095 ). Extra small virus (XSV) usually associated with Mr NV, was detected in some but not all of the samples. Infectivity tests were performed by feeding P. vannamei with minced tissues from Mr NV-infected M. rosenbergii . The assays were preformed at low salinity of 2 ppt and at two different water temperatures of approximately 22 °C and 28 °C. It was revealed that shrimp exhibited a higher infectivity and mortality at the lower temperature. Our findings suggested that P. vannamei is an additional species that is susceptible to Mr NV and that low temperature together with low salinity of rearing water may increase the severity of infections leading to significant mortality.

Kornsunee Phiwsaiya - One of the best experts on this subject based on the ideXlab platform.

  • infections of mrnv macrobrachium rosenbergii nodavirus in cultivated whiteleg shrimp Penaeus vannamei in asia
    Aquaculture, 2012
    Co-Authors: Saengchan Senapin, Chatlada Jaengsanong, Kornsunee Phiwsaiya, Satit Prasertsri, Kesinee Laisutisan, Niti Chuchird, Chalor Limsuwan
    Abstract:

    Abstract Macrobrachium rosenbergii nodavirus ( Mr NV) is well-known as a major pathogen that causes whitened muscles and high mortality in the freshwater prawn Macrobrachium rosenbergii . Recently, it has also been reported to cause white muscles and high mortality in postlarvae of the marine shrimp Penaeus ( Penaeus ) monodon and Penaeus ( FenneroPenaeus ) indicus in India. The latter report stimulated us to re-examine specimens from Asian shrimp farms that had experienced high mortality in Penaeus ( LitoPenaeus ) vannamei with white muscles but tested negative for infectious myonecrosis virus (IMNV) and Penaeus vannamei nodavirus ( Pv NV) by RT-PCR analysis. Some of these specimens were indeed positive for Mr NV by RT-PCR. Sequences of the two single-stranded RNA fragments in the Mr NV genome were amplified from these P. vannamei specimens and found to share ~ 97% nucleic acid sequence identity with the Mr NV sequences deposited at GenBank ( NC_005094 and NC_005095 NC_005094 NC_005095 ). Extra small virus (XSV) usually associated with Mr NV, was detected in some but not all of the samples. Infectivity tests were performed by feeding P. vannamei with minced tissues from Mr NV-infected M. rosenbergii . The assays were preformed at low salinity of 2 ppt and at two different water temperatures of approximately 22 °C and 28 °C. It was revealed that shrimp exhibited a higher infectivity and mortality at the lower temperature. Our findings suggested that P. vannamei is an additional species that is susceptible to Mr NV and that low temperature together with low salinity of rearing water may increase the severity of infections leading to significant mortality.

Chatlada Jaengsanong - One of the best experts on this subject based on the ideXlab platform.

  • infections of mrnv macrobrachium rosenbergii nodavirus in cultivated whiteleg shrimp Penaeus vannamei in asia
    Aquaculture, 2012
    Co-Authors: Saengchan Senapin, Chatlada Jaengsanong, Kornsunee Phiwsaiya, Satit Prasertsri, Kesinee Laisutisan, Niti Chuchird, Chalor Limsuwan
    Abstract:

    Abstract Macrobrachium rosenbergii nodavirus ( Mr NV) is well-known as a major pathogen that causes whitened muscles and high mortality in the freshwater prawn Macrobrachium rosenbergii . Recently, it has also been reported to cause white muscles and high mortality in postlarvae of the marine shrimp Penaeus ( Penaeus ) monodon and Penaeus ( FenneroPenaeus ) indicus in India. The latter report stimulated us to re-examine specimens from Asian shrimp farms that had experienced high mortality in Penaeus ( LitoPenaeus ) vannamei with white muscles but tested negative for infectious myonecrosis virus (IMNV) and Penaeus vannamei nodavirus ( Pv NV) by RT-PCR analysis. Some of these specimens were indeed positive for Mr NV by RT-PCR. Sequences of the two single-stranded RNA fragments in the Mr NV genome were amplified from these P. vannamei specimens and found to share ~ 97% nucleic acid sequence identity with the Mr NV sequences deposited at GenBank ( NC_005094 and NC_005095 NC_005094 NC_005095 ). Extra small virus (XSV) usually associated with Mr NV, was detected in some but not all of the samples. Infectivity tests were performed by feeding P. vannamei with minced tissues from Mr NV-infected M. rosenbergii . The assays were preformed at low salinity of 2 ppt and at two different water temperatures of approximately 22 °C and 28 °C. It was revealed that shrimp exhibited a higher infectivity and mortality at the lower temperature. Our findings suggested that P. vannamei is an additional species that is susceptible to Mr NV and that low temperature together with low salinity of rearing water may increase the severity of infections leading to significant mortality.