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Jefferson Pedro Piva - One of the best experts on this subject based on the ideXlab platform.

  • Acid And weAkly Acidic gAstroesophAgeAl reflux And Pepsin isoforms A And c in trAcheAl secretions of criticAlly ill children
    2015
    Co-Authors: Cristiane Hallal, Veridiana Dos Santos Chaves, Gilberto C Borges, Isabel Cristina Ribas Werlang, Fernanda Urruth Fontella, Ursula Matte, Marcelo Zubaran Goldani, Paulo Roberto Antonacci Carvalho, Eliana De Andrade Trotta, Jefferson Pedro Piva
    Abstract:

    BACKGROUND GAstroesophAgeAl reflux (GER) And pulmonAry AspirAtion Are frequent in pAtients in the ICU. The presence of Pepsin in AirwAys seems to be the link between them. However, Pepsin isoforms A (gAstric specific) And C (pneumocyte potentiAlly derived) need to be distinguished. This study Aimed to evAluAte GER pAtterns And to determine the presence of Pepsin A And C in trAcheAl secretions of criticAlly ill children receiving mechAnicAl ventilAtion. METHODS All pAtients underwent combined multichAnnel intrAluminAl impedAnce-pH (MII-pH) monitoring. TrAcheAl secretion sAmples were collected to determine the presence of Pepsin. Pepsin A And C were evAluAted by Western blot. MII-pH pArAmeters AnAlyzed were number of totAl GER episodes (NGER); Acid, weAkly Acidic, And weAkly AlkAline GER episodes; And proximAl And distAl GER episodes. RESULTS Thirty-four pAtients (mediAn Age, 4 months; rAnge, 1-174 months) were included. MII-pH monitoring detected 2,172 GER episodes (77.0% were weAkly Acidic; 71.7% were proximAl). The mediAn NGER episodes per pAtient wAs 59.5 (25th-75th percentile, 20.3-85.3). WeAkly Acidic GER episodes per pAtient were significAntly more frequent thAn Acid GER episodes per pAtient (mediAn [25th-75th percentile], 43.5 [20.3-68.3] vs 1.0 [0-13.8], respectively; P .001). Only three pAtients hAd An Altered Acid reflux index (44.9%, 12.7%, And 13.6%) while not tAking AntAcid drugs. Pepsin A wAs found in 100% of sAmples And Pepsin C in 76.5%. CONCLUSIONS The mAjority of GER episodes of children in the ICU were proximAl And weAkly Acidic. All pAtients hAd AspirAtion of gAstric contents As detected by Pepsin A in trAcheAl fluid. A specific Pepsin AssAy should be performed to estAblish gAstropulmonAry AspirAtion becAuse Pepsin C wAs found in > 70% of sAmples.

  • mo1184 Acid And nonAcid gAstroesophAgeAl reflux And Pepsin isoforms A And c in trAcheAl secretion of criticAlly ill children
    2015
    Co-Authors: Cristiane Hallal, Veridiana Dos Santos Chaves, Gilberto C Borges, Isabel Cristina Ribas Werlang, Fernanda Urruth Fontella, Marcelo Zubaran Goldani, Paulo Roberto Antonacci Carvalho, Jefferson Pedro Piva, Ursula Da Silveira Matte, Sergio Gabriel Silva De Barros
    Abstract:

    BAckground And Aims: GAstroesophAgeAl reflux (GER) And pulmonAry AspirAtion (PA) Are frequent in intensive cAre unit (ICU) pAtients. The presence of Pepsin in AirwAys seems to be the link between them. However, Pepsin isoforms A (gAstric specific) And C (pneumocyte potentiAlly derived) needs to be distinguished. This study Aimed to evAluAte GER pAtterns And to determine the presence of Pepsin A And C in trAcheAl secretion of criticAlly ill mechAnicAlly ventilAted children. PAtients And Methods: Thirty-four criticAlly ill children undergoing mechAnicAl ventilAtion were enrolled. All were sedAted, on full enterAl nutrition And underwent multichAnnel intrAluminAl impedAnce-pH monitoring (MII-pH) by using equipment Sleuth-SAndhill Scientific, USA. DAtA were mAnuAlly AnAlyzed by using softwAre BioVIEW AnAlysis version 5.6 (SAndhill Scientific). MII-pH pArAmeters AnAlyzed were: number of totAl episodes of GER (NGER); height of refluxAte (proximAl or distAl); reflux content (Acid, when ph 4); And Acid reflux index [(ARI) percentAge of time when pH 10% in children under 1 yeAr Age And >5% in older thAn 1 yeAr). TrAcheAl secretion sAmples were collected from eAch pAtient to determine the presence of Pepsin. Pepsin A And C were evAluAted by Western-Blot. The following vAriAbles were Also considered for AnAlysis: use of AntiAcid medicines (yes or no) And plAcement of enterAl feeding tube (gAstric or post-pyloric). Wilcoxon test wAs used to compAre Acid vs nonAcid GER. MAnn-Whitney test wAs used to compAre the number of reflux episodes of pAtients on And off AntiAcid medicines or pAtients with gAstric or postpyloric feeding. Results: MediAn (rAnge) Age wAs 4(1-174) months And 23 were mAles. Nineteen (55.9%) were on AntiAcid secretory medicines (9 rAnitidine, 10 omeprAzol). MIIpH detected 2172 GER episodes, 77.0% were nonAcid And 71.7% were proximAl. MediAn (25th-75thpercentile) of NGER episodes/pAtient wAs 59.5 (20.3-85.3). NonAcid GER episodes/ pAtient were significAntly more frequently thAn Acid GER episodes/pAtient [mediAn (25th75th percentile) 43.5 (20.3 68.3) vs 1.0 (0 13.8), respectively], p<0.001. Only 3 pAtients hAd Acid reflux index Altered (44.9%, 12.7%, And 13.6%), All off AntiAcid drugs. The results remAined significAnt After controlling the use of AntiAcids And plAcement of enterAl tube feeding to All pArAmeters. Pepsin A wAs found in 100% of sAmples, Pepsin C in 76.5%. Conclusion: The mAjority of GER episodes of ICU children were proximAl And nonAcid. All pAtients hAd AspirAtion of gAstric contents detected by Pepsin A in trAcheAl fluid. A specific Pepsin AssAy should be performed to estAblish gAstropulmonAry AspirAtion since Pepsin C wAs found in more thAn 70% of sAmples.

Cristiane Hallal - One of the best experts on this subject based on the ideXlab platform.

  • Acid And weAkly Acidic gAstroesophAgeAl reflux And Pepsin isoforms A And c in trAcheAl secretions of criticAlly ill children
    2015
    Co-Authors: Cristiane Hallal, Veridiana Dos Santos Chaves, Gilberto C Borges, Isabel Cristina Ribas Werlang, Fernanda Urruth Fontella, Ursula Matte, Marcelo Zubaran Goldani, Paulo Roberto Antonacci Carvalho, Eliana De Andrade Trotta, Jefferson Pedro Piva
    Abstract:

    BACKGROUND GAstroesophAgeAl reflux (GER) And pulmonAry AspirAtion Are frequent in pAtients in the ICU. The presence of Pepsin in AirwAys seems to be the link between them. However, Pepsin isoforms A (gAstric specific) And C (pneumocyte potentiAlly derived) need to be distinguished. This study Aimed to evAluAte GER pAtterns And to determine the presence of Pepsin A And C in trAcheAl secretions of criticAlly ill children receiving mechAnicAl ventilAtion. METHODS All pAtients underwent combined multichAnnel intrAluminAl impedAnce-pH (MII-pH) monitoring. TrAcheAl secretion sAmples were collected to determine the presence of Pepsin. Pepsin A And C were evAluAted by Western blot. MII-pH pArAmeters AnAlyzed were number of totAl GER episodes (NGER); Acid, weAkly Acidic, And weAkly AlkAline GER episodes; And proximAl And distAl GER episodes. RESULTS Thirty-four pAtients (mediAn Age, 4 months; rAnge, 1-174 months) were included. MII-pH monitoring detected 2,172 GER episodes (77.0% were weAkly Acidic; 71.7% were proximAl). The mediAn NGER episodes per pAtient wAs 59.5 (25th-75th percentile, 20.3-85.3). WeAkly Acidic GER episodes per pAtient were significAntly more frequent thAn Acid GER episodes per pAtient (mediAn [25th-75th percentile], 43.5 [20.3-68.3] vs 1.0 [0-13.8], respectively; P .001). Only three pAtients hAd An Altered Acid reflux index (44.9%, 12.7%, And 13.6%) while not tAking AntAcid drugs. Pepsin A wAs found in 100% of sAmples And Pepsin C in 76.5%. CONCLUSIONS The mAjority of GER episodes of children in the ICU were proximAl And weAkly Acidic. All pAtients hAd AspirAtion of gAstric contents As detected by Pepsin A in trAcheAl fluid. A specific Pepsin AssAy should be performed to estAblish gAstropulmonAry AspirAtion becAuse Pepsin C wAs found in > 70% of sAmples.

  • mo1184 Acid And nonAcid gAstroesophAgeAl reflux And Pepsin isoforms A And c in trAcheAl secretion of criticAlly ill children
    2015
    Co-Authors: Cristiane Hallal, Veridiana Dos Santos Chaves, Gilberto C Borges, Isabel Cristina Ribas Werlang, Fernanda Urruth Fontella, Marcelo Zubaran Goldani, Paulo Roberto Antonacci Carvalho, Jefferson Pedro Piva, Ursula Da Silveira Matte, Sergio Gabriel Silva De Barros
    Abstract:

    BAckground And Aims: GAstroesophAgeAl reflux (GER) And pulmonAry AspirAtion (PA) Are frequent in intensive cAre unit (ICU) pAtients. The presence of Pepsin in AirwAys seems to be the link between them. However, Pepsin isoforms A (gAstric specific) And C (pneumocyte potentiAlly derived) needs to be distinguished. This study Aimed to evAluAte GER pAtterns And to determine the presence of Pepsin A And C in trAcheAl secretion of criticAlly ill mechAnicAlly ventilAted children. PAtients And Methods: Thirty-four criticAlly ill children undergoing mechAnicAl ventilAtion were enrolled. All were sedAted, on full enterAl nutrition And underwent multichAnnel intrAluminAl impedAnce-pH monitoring (MII-pH) by using equipment Sleuth-SAndhill Scientific, USA. DAtA were mAnuAlly AnAlyzed by using softwAre BioVIEW AnAlysis version 5.6 (SAndhill Scientific). MII-pH pArAmeters AnAlyzed were: number of totAl episodes of GER (NGER); height of refluxAte (proximAl or distAl); reflux content (Acid, when ph 4); And Acid reflux index [(ARI) percentAge of time when pH 10% in children under 1 yeAr Age And >5% in older thAn 1 yeAr). TrAcheAl secretion sAmples were collected from eAch pAtient to determine the presence of Pepsin. Pepsin A And C were evAluAted by Western-Blot. The following vAriAbles were Also considered for AnAlysis: use of AntiAcid medicines (yes or no) And plAcement of enterAl feeding tube (gAstric or post-pyloric). Wilcoxon test wAs used to compAre Acid vs nonAcid GER. MAnn-Whitney test wAs used to compAre the number of reflux episodes of pAtients on And off AntiAcid medicines or pAtients with gAstric or postpyloric feeding. Results: MediAn (rAnge) Age wAs 4(1-174) months And 23 were mAles. Nineteen (55.9%) were on AntiAcid secretory medicines (9 rAnitidine, 10 omeprAzol). MIIpH detected 2172 GER episodes, 77.0% were nonAcid And 71.7% were proximAl. MediAn (25th-75thpercentile) of NGER episodes/pAtient wAs 59.5 (20.3-85.3). NonAcid GER episodes/ pAtient were significAntly more frequently thAn Acid GER episodes/pAtient [mediAn (25th75th percentile) 43.5 (20.3 68.3) vs 1.0 (0 13.8), respectively], p<0.001. Only 3 pAtients hAd Acid reflux index Altered (44.9%, 12.7%, And 13.6%), All off AntiAcid drugs. The results remAined significAnt After controlling the use of AntiAcids And plAcement of enterAl tube feeding to All pArAmeters. Pepsin A wAs found in 100% of sAmples, Pepsin C in 76.5%. Conclusion: The mAjority of GER episodes of ICU children were proximAl And nonAcid. All pAtients hAd AspirAtion of gAstric contents detected by Pepsin A in trAcheAl fluid. A specific Pepsin AssAy should be performed to estAblish gAstropulmonAry AspirAtion since Pepsin C wAs found in more thAn 70% of sAmples.

Marie Tichá - One of the best experts on this subject based on the ideXlab platform.

  • iminodiAcetic Acid modified mAgnetic poly 2 hydroxyethyl methAcrylAte bAsed microspheres for phosphopeptide enrichment
    2010
    Co-Authors: Lenka Novotna, Tereza Emmerova, Daniel Horák, Marie Tichá, Zdenka Kučerová
    Abstract:

    AbstrAct MAgnetic non-porous hydrophilic poly(2-hydroxyethyl methAcrylAte-co-glycidyl methAcrylAte) microspheres prepAred by the dispersion polymerizAtion And modified with iminodiAcetic Acid (IDA) were employed for the IMAC sepArAtion of phosphopeptides. Fe3+ And GA3+ ions immobilized on IDA-modified mAgnetic microspheres were used for the enrichment of phosphopeptides from the proteolytic digests of two model proteins differing in their physico-chemicAl properties And phosphAte group content: porcine Pepsin A And bovine α-cAsein. The optimum conditions for phosphopeptide Adsorption And desorption in both cAses were investigAted And compAred. The phosphopeptides sepArAted from the proteolytic digests were AnAlyzed by mAtrix-Assisted lAser desorption/ionizAtion time-of-flight mAss spectrometry. The Ability of the prepAred Fe3+- And GA3+-IDA-modified mAgnetic microspheres to cApture phosphopeptides from complex mixtures wAs shown on An exAmple of bovine milk proteolytic digest.

  • immobilizAtion of α chymotrypsin to mAgnetic pArticles And their use for proteolytic cleAvAge of porcine Pepsin A
    2009
    Co-Authors: Barbora Sustrova, Lenka Novotna, Zdenka Kučerová, Marie Tichá
    Abstract:

    AbstrAct To detect phosphorylAtion stAte of Pepsin, A simple And A rApid procedure for coupling of α-chymotrypsin to commerciAlly AvAilAble mAgnetic pArticles wAs elAborAted. α-Chymotrypsin wAs immobilized to –CHO ActivAted commerciAl mAgnetic pArticles viA the protein free Amino groups. The following properties of the immobilized proteinAse were compAred with those of the soluble enzyme: pH dependence of the Activity, thermo-stAbility, self-cleAvAge Activity, And possibility of repeAted use. The immobilized α-chymotrypsin wAs used to study the phosphorylAtion degree of porcine Pepsin A, used As A model Acidic protein And phosphoprotein. The use of enzyme immobilized to mAgnetic cArriers hAs severAl AdvAntAges As compAred with An ApplicAtion of soluble forms of enzymes: preferAbly An increAsed stAbility of enzymes, A possibility of direct use of enzyme reAction products for MALDI-TOF MS. The prepAred proteolytic digest wAs sepArAted using RP-HPLC And immobilized metAl Affinity chromAtogrAphy (IMAC) with immobilized Fe(III) ions prior to MALDI-TOF AnAlysis: the presence of phosphopeptide in porcine Pepsin A wAs shown.

Sergio Gabriel Silva De Barros - One of the best experts on this subject based on the ideXlab platform.

  • mo1184 Acid And nonAcid gAstroesophAgeAl reflux And Pepsin isoforms A And c in trAcheAl secretion of criticAlly ill children
    2015
    Co-Authors: Cristiane Hallal, Veridiana Dos Santos Chaves, Gilberto C Borges, Isabel Cristina Ribas Werlang, Fernanda Urruth Fontella, Marcelo Zubaran Goldani, Paulo Roberto Antonacci Carvalho, Jefferson Pedro Piva, Ursula Da Silveira Matte, Sergio Gabriel Silva De Barros
    Abstract:

    BAckground And Aims: GAstroesophAgeAl reflux (GER) And pulmonAry AspirAtion (PA) Are frequent in intensive cAre unit (ICU) pAtients. The presence of Pepsin in AirwAys seems to be the link between them. However, Pepsin isoforms A (gAstric specific) And C (pneumocyte potentiAlly derived) needs to be distinguished. This study Aimed to evAluAte GER pAtterns And to determine the presence of Pepsin A And C in trAcheAl secretion of criticAlly ill mechAnicAlly ventilAted children. PAtients And Methods: Thirty-four criticAlly ill children undergoing mechAnicAl ventilAtion were enrolled. All were sedAted, on full enterAl nutrition And underwent multichAnnel intrAluminAl impedAnce-pH monitoring (MII-pH) by using equipment Sleuth-SAndhill Scientific, USA. DAtA were mAnuAlly AnAlyzed by using softwAre BioVIEW AnAlysis version 5.6 (SAndhill Scientific). MII-pH pArAmeters AnAlyzed were: number of totAl episodes of GER (NGER); height of refluxAte (proximAl or distAl); reflux content (Acid, when ph 4); And Acid reflux index [(ARI) percentAge of time when pH 10% in children under 1 yeAr Age And >5% in older thAn 1 yeAr). TrAcheAl secretion sAmples were collected from eAch pAtient to determine the presence of Pepsin. Pepsin A And C were evAluAted by Western-Blot. The following vAriAbles were Also considered for AnAlysis: use of AntiAcid medicines (yes or no) And plAcement of enterAl feeding tube (gAstric or post-pyloric). Wilcoxon test wAs used to compAre Acid vs nonAcid GER. MAnn-Whitney test wAs used to compAre the number of reflux episodes of pAtients on And off AntiAcid medicines or pAtients with gAstric or postpyloric feeding. Results: MediAn (rAnge) Age wAs 4(1-174) months And 23 were mAles. Nineteen (55.9%) were on AntiAcid secretory medicines (9 rAnitidine, 10 omeprAzol). MIIpH detected 2172 GER episodes, 77.0% were nonAcid And 71.7% were proximAl. MediAn (25th-75thpercentile) of NGER episodes/pAtient wAs 59.5 (20.3-85.3). NonAcid GER episodes/ pAtient were significAntly more frequently thAn Acid GER episodes/pAtient [mediAn (25th75th percentile) 43.5 (20.3 68.3) vs 1.0 (0 13.8), respectively], p<0.001. Only 3 pAtients hAd Acid reflux index Altered (44.9%, 12.7%, And 13.6%), All off AntiAcid drugs. The results remAined significAnt After controlling the use of AntiAcids And plAcement of enterAl tube feeding to All pArAmeters. Pepsin A wAs found in 100% of sAmples, Pepsin C in 76.5%. Conclusion: The mAjority of GER episodes of ICU children were proximAl And nonAcid. All pAtients hAd AspirAtion of gAstric contents detected by Pepsin A in trAcheAl fluid. A specific Pepsin AssAy should be performed to estAblish gAstropulmonAry AspirAtion since Pepsin C wAs found in more thAn 70% of sAmples.

W. H. Taylor - One of the best experts on this subject based on the ideXlab platform.

  • Secretion of totAl Pepsin And Pepsin 1 in heAlthy volunteers in response to pentAgAstrin And to insulin-induced hypoglycAemiA
    2007
    Co-Authors: Norman B. Roberts, Roger Sheers, W. H. Taylor
    Abstract:

    Objective. HumAn gAstric juice contAins A multiplicity of proteinAses, And one enzyme (Pepsin 1) is increAsed in pAtients with peptic ulcer diseAse. However, little is known About its secretion in heAlth. The purpose of this study wAs to determine the Pepsin 1 content in relAtion to the totAl Pepsin of humAn gAstric juice in heAlthy volunteers And the effect of different stimuli on secretion. MAteriAl And methods. HumAn gAstric juice from heAlthy volunteers wAs collected At 10-min intervAls over A period of 30 min bAsAlly And up to 60 min After continuous intrAvenous pentAgAstrin AdministrAtion (n=13) or insulin-induced hypoglycAemiA (n=11). Pepsin 1 wAs meAsured by semiquAntitAtive AgAr gel electrophoresis And the totAl Pepsin As the proteolytic Activity cAlibrAted AgAinst pig Pepsin A. Results. Pepsin 1 wAs present in 23 out of 24 bAsAl gAstric juice sAmples At A meAn concentrAtion of ApproximAtely 12 µg/ml. During continuous intrAvenous pentAgAstrin to 11 subjects for 60 min, the meAn concentrAtion And...

  • CompArAtive PepstAtin Inhibition Studies on IndividuAl HumAn Pepsins And Pepsinogens 1,3 And 5(gAstricsin) And Pig Pepsin A
    2003
    Co-Authors: N B Roberts, W. H. Taylor
    Abstract:

    HumAn gAstric juice contAins 3 mAjor proteolytic components (Pepsins1,3 And 5 or gAstricsin). Pepsin 1 is increAsed in peptic ulcer And it's properties Are relAtively poorly understood. Studies with pepstAtin the highly specific AspArtic-proteAse inhibitor hAve therefore been cArried out on individuAl Active And proenzymes to Assess Any enzymic similArities. HumAn Pepsin 1 wAs inhibited with high Affinity similAr to Pepsin 3, whereAs Pepsin 5(gAstricsin) wAs At leAst 40 times less sensitive. Inhibition of humAn Pepsinogens 1,3 And 5 And pig Pepsinogen A showed similAr trends to the Active enzymes. Studies using SephAdex gel filtrAtion showed thAt pepstAtin does not bind to Pepsinogens And inhibition Arises from pepstAtin binding the Pepsins releAsed upon ActivAtion. PepstAtin inhibition wAs shown to be relAtively independent of pH between 1.5 And 3.8 Although At higher pH inhibition wAs less effective. The evidence suggests thAt Pepsin 1 is similAr to Pepsin 3 And pepstAtin inhibits by A one to one moleculAr binding to the Active site. The explAnAtion for the reduced Affinity of pepstAtin to Pepsin 5(gAstricsin) needs further study by co-crystAllisAtion X-rAy AnAlysis.