The Experts below are selected from a list of 225 Experts worldwide ranked by ideXlab platform
Maria C Jimenezmartinez - One of the best experts on this subject based on the ideXlab platform.
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low expression of il 10 in circulating bregs and inverted il 10 tnf α ratio in tears of patients with Perennial Allergic Conjunctivitis a preliminary study
International Journal of Molecular Sciences, 2019Co-Authors: Alberto Salazar, Julio Ayalabalboa, Israel Casanovamendez, Michele Pachecoquito, Henry Velazquezsoto, Enrique O Grauehernandez, Jeanet Serafinlopez, Maria C JimenezmartinezAbstract:Allergic Conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset of IL-10-expressing B cells is involved in inflammatory Allergic diseases. In this study, we aimed to evaluate the potential involvement of blood Bregs cells in Perennial Allergic Conjunctivitis (PAC), and interleukins (IL)-1β, IL-6, IL-8, IL-10, and IL-12, and tumor necrosis factor (TNF)-α, were measured in tear samples and compared with healthy controls (HC) using flow cytometry. Non-significant differences in CD19+IL-10+ cell frequency between PAC patients and healthy controls (HC) were observed. Nevertheless, when we analyzed the mean fluorescence intensity (MFI) of IL-10 on CD19+CD38Lo/Med/Hi-gated cells, we observed a significant decrease in MFI in all Bregs subsets in PAC patients. Additionally, tear cytokines showed 2.8 times lower levels of IL-10 than TNF-α in PAC patients when compared to HC. Our findings demonstrate an immunological dysregulation in patients with Allergic Conjunctivitis, characterized by the low expression of IL-10 in circulating CD19+CD38+ Bregs subsets and an inverted tear IL-10/TNF-α ratio, promoting a local pro-inflammatory microenvironment. These findings highlight the novel pathologic changes involved in ocular Allergic diseases. Understanding systemic and local mechanisms will aid the design of immunomodulating therapeutics at different levels.
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an imbalance between frequency of cd4 cd25 foxp3 regulatory t cells and ccr4 and ccr9 circulating helper t cells is associated with active Perennial Allergic Conjunctivitis
Clinical & Developmental Immunology, 2013Co-Authors: Jorge Galiciacarreon, Concepcion Santacruz, Julio Ayalabalboa, Atzin Roblescontreras, Sonia Mayra Pereztapia, Yonathan Garfias, E Hong, Maria C JimenezmartinezAbstract:Allergic Conjunctivitis (AC) is one of the most common eye disorders in ophthalmology. In mice models, it has been suggested that control of Allergic Conjunctivitis is a delicate balance between Tregs and inflammatory migrating effector cells. Our aim was to evaluate the frequency of Tregs and the frequency of homing receptors expressing cells in peripheral blood mononuclear cells (PBMC) from patients with Perennial Allergic Conjunctivitis (PAC). The analyses of phenotypic markers on CD4+ T cells and both soluble or intracellular cytokines were performed by flow cytometry. CD4+CD25+ cells were 15 times more frequent in PBMC from patients than HC; the vast majority of these CD4+CD25+ cells were FOXP3-, and most of CD4+ T cells were CCR4+ and CCR9+ cells. Upon allergen-stimulation, no significant changes were observed in frequency of Treg; however, an increased frequency of CD4+CCR4+CCR9+ cells, CD4+CD103+ cells and CD4+CD108+ cells with increased IL-5, IL-6, and IL-8 production was observed. These findings suggest an immune dysregulation in PAC, characterized by diminished frequency of Tregs and increased frequency of circulating activated CD4+ T cells; upon allergen-stimulation, these cells were expressing cell-surface molecules related to mucosa homing and were able to trigger an inflammatory microenvironment.
Bing Li - One of the best experts on this subject based on the ideXlab platform.
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expression of tslp and downstream molecules il 4 il 5 and il 13 on the eye surface of patients with various types of Allergic Conjunctivitis
Journal of Ophthalmology, 2016Co-Authors: Xiaofen Zheng, Bing LiAbstract:Background. The pathogenesis of Allergic Conjunctivitis has not been clearly established. Moreover, previous studies fail to consider human models of Allergic Conjunctivitis. This study investigated the expression of thymic stromal lymphopoiet in TSLP and its downstream molecules in conjunctival scrappings and tear. Methods. This cross-sectional study compares patients with vernal keratoConjunctivitis (VKC), seasonal Allergic Conjunctivitis (SAC), and Perennial Allergic Conjunctivitis (PAC) with normal controls. There are 80 people recorded in Shanxi Eye Hospital. Increasingly, 20 are with VKC, 20 are with SAC, 20 are with PAC, and the remaining 20 are normal controls. Conjunctiva were harvested for total RNA extraction and gene expression by real-time polymerase chain reaction. Epithelial cells were collected to make pathological sections for immunohistochemical staining. Human tears were evaluated by Luminex microbead assay. A value less than 0.05 from Dunnett’s post hoc test in SPSS means a statistical significant distinction. Results. Positive expression in conjunctival cells of patients with Allergic Conjunctivitis. The expression of TSLP and IL-4, IL-5, and IL-13 mRNA shows a statistically significant difference (). TSLP and IL-4, IL-5, and IL-13 concentrations show a statistically significant difference (). Conclusions. This study suggests that TSLP and downstream molecules are expressed in patients with various types of Allergic Conjunctivitis.
Julio Ayalabalboa - One of the best experts on this subject based on the ideXlab platform.
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low expression of il 10 in circulating bregs and inverted il 10 tnf α ratio in tears of patients with Perennial Allergic Conjunctivitis a preliminary study
International Journal of Molecular Sciences, 2019Co-Authors: Alberto Salazar, Julio Ayalabalboa, Israel Casanovamendez, Michele Pachecoquito, Henry Velazquezsoto, Enrique O Grauehernandez, Jeanet Serafinlopez, Maria C JimenezmartinezAbstract:Allergic Conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset of IL-10-expressing B cells is involved in inflammatory Allergic diseases. In this study, we aimed to evaluate the potential involvement of blood Bregs cells in Perennial Allergic Conjunctivitis (PAC), and interleukins (IL)-1β, IL-6, IL-8, IL-10, and IL-12, and tumor necrosis factor (TNF)-α, were measured in tear samples and compared with healthy controls (HC) using flow cytometry. Non-significant differences in CD19+IL-10+ cell frequency between PAC patients and healthy controls (HC) were observed. Nevertheless, when we analyzed the mean fluorescence intensity (MFI) of IL-10 on CD19+CD38Lo/Med/Hi-gated cells, we observed a significant decrease in MFI in all Bregs subsets in PAC patients. Additionally, tear cytokines showed 2.8 times lower levels of IL-10 than TNF-α in PAC patients when compared to HC. Our findings demonstrate an immunological dysregulation in patients with Allergic Conjunctivitis, characterized by the low expression of IL-10 in circulating CD19+CD38+ Bregs subsets and an inverted tear IL-10/TNF-α ratio, promoting a local pro-inflammatory microenvironment. These findings highlight the novel pathologic changes involved in ocular Allergic diseases. Understanding systemic and local mechanisms will aid the design of immunomodulating therapeutics at different levels.
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an imbalance between frequency of cd4 cd25 foxp3 regulatory t cells and ccr4 and ccr9 circulating helper t cells is associated with active Perennial Allergic Conjunctivitis
Clinical & Developmental Immunology, 2013Co-Authors: Jorge Galiciacarreon, Concepcion Santacruz, Julio Ayalabalboa, Atzin Roblescontreras, Sonia Mayra Pereztapia, Yonathan Garfias, E Hong, Maria C JimenezmartinezAbstract:Allergic Conjunctivitis (AC) is one of the most common eye disorders in ophthalmology. In mice models, it has been suggested that control of Allergic Conjunctivitis is a delicate balance between Tregs and inflammatory migrating effector cells. Our aim was to evaluate the frequency of Tregs and the frequency of homing receptors expressing cells in peripheral blood mononuclear cells (PBMC) from patients with Perennial Allergic Conjunctivitis (PAC). The analyses of phenotypic markers on CD4+ T cells and both soluble or intracellular cytokines were performed by flow cytometry. CD4+CD25+ cells were 15 times more frequent in PBMC from patients than HC; the vast majority of these CD4+CD25+ cells were FOXP3-, and most of CD4+ T cells were CCR4+ and CCR9+ cells. Upon allergen-stimulation, no significant changes were observed in frequency of Treg; however, an increased frequency of CD4+CCR4+CCR9+ cells, CD4+CD103+ cells and CD4+CD108+ cells with increased IL-5, IL-6, and IL-8 production was observed. These findings suggest an immune dysregulation in PAC, characterized by diminished frequency of Tregs and increased frequency of circulating activated CD4+ T cells; upon allergen-stimulation, these cells were expressing cell-surface molecules related to mucosa homing and were able to trigger an inflammatory microenvironment.
Xiaofen Zheng - One of the best experts on this subject based on the ideXlab platform.
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expression of tslp and downstream molecules il 4 il 5 and il 13 on the eye surface of patients with various types of Allergic Conjunctivitis
Journal of Ophthalmology, 2016Co-Authors: Xiaofen Zheng, Bing LiAbstract:Background. The pathogenesis of Allergic Conjunctivitis has not been clearly established. Moreover, previous studies fail to consider human models of Allergic Conjunctivitis. This study investigated the expression of thymic stromal lymphopoiet in TSLP and its downstream molecules in conjunctival scrappings and tear. Methods. This cross-sectional study compares patients with vernal keratoConjunctivitis (VKC), seasonal Allergic Conjunctivitis (SAC), and Perennial Allergic Conjunctivitis (PAC) with normal controls. There are 80 people recorded in Shanxi Eye Hospital. Increasingly, 20 are with VKC, 20 are with SAC, 20 are with PAC, and the remaining 20 are normal controls. Conjunctiva were harvested for total RNA extraction and gene expression by real-time polymerase chain reaction. Epithelial cells were collected to make pathological sections for immunohistochemical staining. Human tears were evaluated by Luminex microbead assay. A value less than 0.05 from Dunnett’s post hoc test in SPSS means a statistical significant distinction. Results. Positive expression in conjunctival cells of patients with Allergic Conjunctivitis. The expression of TSLP and IL-4, IL-5, and IL-13 mRNA shows a statistically significant difference (). TSLP and IL-4, IL-5, and IL-13 concentrations show a statistically significant difference (). Conclusions. This study suggests that TSLP and downstream molecules are expressed in patients with various types of Allergic Conjunctivitis.
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Expression of TSLP and Downstream Molecules IL-4, IL-5, and IL-13 on the Eye Surface of Patients with Various Types of Allergic Conjunctivitis
Hindawi Limited, 2016Co-Authors: Xiaofen Zheng, Juan YaoAbstract:Background. The pathogenesis of Allergic Conjunctivitis has not been clearly established. Moreover, previous studies fail to consider human models of Allergic Conjunctivitis. This study investigated the expression of thymic stromal lymphopoiet in TSLP and its downstream molecules in conjunctival scrappings and tear. Methods. This cross-sectional study compares patients with vernal keratoConjunctivitis (VKC), seasonal Allergic Conjunctivitis (SAC), and Perennial Allergic Conjunctivitis (PAC) with normal controls. There are 80 people recorded in Shanxi Eye Hospital. Increasingly, 20 are with VKC, 20 are with SAC, 20 are with PAC, and the remaining 20 are normal controls. Conjunctiva were harvested for total RNA extraction and gene expression by real-time polymerase chain reaction. Epithelial cells were collected to make pathological sections for immunohistochemical staining. Human tears were evaluated by Luminex microbead assay. A P value less than 0.05 from Dunnett’s post hoc test in SPSS means a statistical significant distinction. Results. Positive expression in conjunctival cells of patients with Allergic Conjunctivitis. The expression of TSLP and IL-4, IL-5, and IL-13 mRNA shows a statistically significant difference (P
Alberto Salazar - One of the best experts on this subject based on the ideXlab platform.
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low expression of il 10 in circulating bregs and inverted il 10 tnf α ratio in tears of patients with Perennial Allergic Conjunctivitis a preliminary study
International Journal of Molecular Sciences, 2019Co-Authors: Alberto Salazar, Julio Ayalabalboa, Israel Casanovamendez, Michele Pachecoquito, Henry Velazquezsoto, Enrique O Grauehernandez, Jeanet Serafinlopez, Maria C JimenezmartinezAbstract:Allergic Conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset of IL-10-expressing B cells is involved in inflammatory Allergic diseases. In this study, we aimed to evaluate the potential involvement of blood Bregs cells in Perennial Allergic Conjunctivitis (PAC), and interleukins (IL)-1β, IL-6, IL-8, IL-10, and IL-12, and tumor necrosis factor (TNF)-α, were measured in tear samples and compared with healthy controls (HC) using flow cytometry. Non-significant differences in CD19+IL-10+ cell frequency between PAC patients and healthy controls (HC) were observed. Nevertheless, when we analyzed the mean fluorescence intensity (MFI) of IL-10 on CD19+CD38Lo/Med/Hi-gated cells, we observed a significant decrease in MFI in all Bregs subsets in PAC patients. Additionally, tear cytokines showed 2.8 times lower levels of IL-10 than TNF-α in PAC patients when compared to HC. Our findings demonstrate an immunological dysregulation in patients with Allergic Conjunctivitis, characterized by the low expression of IL-10 in circulating CD19+CD38+ Bregs subsets and an inverted tear IL-10/TNF-α ratio, promoting a local pro-inflammatory microenvironment. These findings highlight the novel pathologic changes involved in ocular Allergic diseases. Understanding systemic and local mechanisms will aid the design of immunomodulating therapeutics at different levels.
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Low Expression of IL-10 in Circulating Bregs and Inverted IL-10/TNF-α Ratio in Tears of Patients with Perennial Allergic Conjunctivitis: A Preliminary Study
'MDPI AG', 2019Co-Authors: Alberto Salazar, Israel Casanova-méndez, Michele Pacheco-quito, Henry Velázquez-soto, Julio Ayala-balboa, Enrique O. Graue-hernández, Jeanet Serafín-lópez, María C. Jiménez-martínezAbstract:Allergic Conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset of IL-10-expressing B cells is involved in inflammatory Allergic diseases. In this study, we aimed to evaluate the potential involvement of blood Bregs cells in Perennial Allergic Conjunctivitis (PAC), and interleukins (IL)-1β, IL-6, IL-8, IL-10, and IL-12, and tumor necrosis factor (TNF)-α, were measured in tear samples and compared with healthy controls (HC) using flow cytometry. Non-significant differences in CD19+IL-10+ cell frequency between PAC patients and healthy controls (HC) were observed. Nevertheless, when we analyzed the mean fluorescence intensity (MFI) of IL-10 on CD19+CD38Lo/Med/Hi-gated cells, we observed a significant decrease in MFI in all Bregs subsets in PAC patients. Additionally, tear cytokines showed 2.8 times lower levels of IL-10 than TNF-α in PAC patients when compared to HC. Our findings demonstrate an immunological dysregulation in patients with Allergic Conjunctivitis, characterized by the low expression of IL-10 in circulating CD19+CD38+ Bregs subsets and an inverted tear IL-10/TNF-α ratio, promoting a local pro-inflammatory microenvironment. These findings highlight the novel pathologic changes involved in ocular Allergic diseases. Understanding systemic and local mechanisms will aid the design of immunomodulating therapeutics at different levels