The Experts below are selected from a list of 8370 Experts worldwide ranked by ideXlab platform
Tingyu Duan - One of the best experts on this subject based on the ideXlab platform.
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an arbuscular mycorrhizal fungus and epichloe festucae var lolii reduce bipolaris sorokiniana disease incidence and improve Perennial Ryegrass growth
Mycorrhiza, 2018Co-Authors: Fang Li, Michael J Christensen, Yanzhong Li, Tingyu DuanAbstract:Leaf spot of Perennial Ryegrass (Lolium perenne) caused by Bipolaris sorokiniana is an important disease in temperate regions of the world. We designed this experiment to test for the combined effects of the arbuscular mycorrhizal (AM) fungus Claroideoglomus etunicatum and the grass endophyte fungus Epichloe festucae var. lolii on growth and disease occurrence in Perennial Ryegrass. The results show that C. etunicatum increased plant P uptake and total dry weight and that this beneficial effect was slightly enhanced when in association with the grass endophyte. The presence in plants of both the endophyte and B. sorokiniana decreased AM fungal colonization. Plants inoculated with B. sorokiniana showed the typical leaf spot symptoms 2 weeks after inoculation and the lowest disease incidence was with plants that were host to both C. etunicatum and E. festucae var. lolii. Plants with these two fungi had much higher activity of peroxidases (POD), superoxide dismutase (SOD) and catalase (CAT) and lower values of malondialdehyde (MDA) and hydrogen peroxide (H2O2). The AM fungus C. etunicatum and the grass endophyte fungus E. festucae var. lolii have the potential to promote Perennial Ryegrass growth and resistance to B. sorokiniana leaf spot.
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an arbuscular mycorrhizal fungus and epichloe festucae var lolii reduce bipolaris sorokiniana disease incidence and improve Perennial Ryegrass growth
Mycorrhiza, 2018Co-Authors: Yane Guo, Michael J Christensen, Ping Gao, Tingyu DuanAbstract:Leaf spot of Perennial Ryegrass (Lolium perenne) caused by Bipolaris sorokiniana is an important disease in temperate regions of the world. We designed this experiment to test for the combined effects of the arbuscular mycorrhizal (AM) fungus Claroideoglomus etunicatum and the grass endophyte fungus Epichloe festucae var. lolii on growth and disease occurrence in Perennial Ryegrass. The results show that C. etunicatum increased plant P uptake and total dry weight and that this beneficial effect was slightly enhanced when in association with the grass endophyte. The presence in plants of both the endophyte and B. sorokiniana decreased AM fungal colonization. Plants inoculated with B. sorokiniana showed the typical leaf spot symptoms 2 weeks after inoculation and the lowest disease incidence was with plants that were host to both C. etunicatum and E. festucae var. lolii. Plants with these two fungi had much higher activity of peroxidases (POD), superoxide dismutase (SOD) and catalase (CAT) and lower values of malondialdehyde (MDA) and hydrogen peroxide (H2O2). The AM fungus C. etunicatum and the grass endophyte fungus E. festucae var. lolii have the potential to promote Perennial Ryegrass growth and resistance to B. sorokiniana leaf spot.
Yan Xie - One of the best experts on this subject based on the ideXlab platform.
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aspergillus aculeatus enhances potassium uptake and photosynthetic characteristics in Perennial Ryegrass by increasing potassium availability
Journal of Applied Microbiology, 2021Co-Authors: Yanling Yin, Erick Amombo, Shugao Fan, Yan XieAbstract:AIM Potassium (K) is a key determinant for plant development and productivity. However, more than 90% of K in the soil exists in an insoluble form. K-solubilizing microbes play an important role in the transformation of insoluble K. Thus, the objective of this study was to evaluate K-dissolving ability of Aspergillus aculeatus (F) and growth-promoting properties in Perennial Ryegrass. METHODS AND RESULTS Perennial Ryegrass inoculated with A. aculeatus exhibited enhanced soluble K accompanied with higher growth rate and turf quality, compared with the noninoculated regimen. In addition, A. aculeatus also played a primary role in increasing chlorophyll content and photosynthetic capacity of the plant exposed to LK+F (K-feldspar plus A. aculeatus) treatment, compared with the CK (control, no K-feldspar and A. aculeatus), F (only A. aculeatus) and LK (only K-feldspar) groups. Furthermore, the antioxidase activities (CAT and POD) were significantly increased while the oxidative damage (EL and MDA) was dramatically decreased in the LK+F group compared to the LK (K-feldspar) group. Finally, in Perennial Ryegrass leaves, the genes expression levels of HAK8, HAK12 and HKT18 were obviously elevated in the LK+F group, compared to the CK, F and LK groups. CONCLUSION We concluded that A. aculeatus could solubilize K from bound form and be considered as K-solubilizing biofertilizer through supplementing K in soil. SIGNIFICANCE AND IMPACT OF THE STUDY Aspergillus aculeatus has the potential to be used as a biofertilizer in sustainable agriculture.
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Exogenous Aspergillus aculeatus Enhances Drought and Heat Tolerance of Perennial Ryegrass.
Frontiers in microbiology, 2021Co-Authors: Chuncheng Zhao, Erick Amombo, Ting Zhang, Guangyang Wang, Yan XieAbstract:Perennial Ryegrass (Lolium perenne) is a cool-season grass whose growth and development are limited by drought and high temperature. Aspergillus aculeatus has been reported to promote plant growth and counteract the adverse effects of abiotic stresses. The objective of this study was to assess A. aculeatus-induced response mechanisms to drought and heat resistance in Perennial Ryegrass. We evaluated the physiological and biochemical markers of drought and heat stress based on the hormone homeostasis, photosynthesis, antioxidant enzymes activity, lipid peroxidation, and genes expression level. We found out that under drought and heat stress, A. aculeatus-inoculated leaves exhibited higher abscisic acid (ABA) and lower salicylic acid (SA) contents than non-inoculated regimes. In addition, under drought and heat stress, the fungus enhanced the photosynthetic performance, decreased the antioxidase activities, and mitigated membrane lipid peroxidation compared to non-inoculated regime. Furthermore, under drought stress, A. aculeatus induced a dramatic upregulation of sHSP17.8 and DREB1A and a downregulation of POD47, Cu/ZnSOD, and FeSOD genes. In addition, under heat stress, A. aculeatus-inoculated plants exhibited a higher expression level of HSP26.7a, sHSP17.8, and DREB1A while a lower expression level of POD47 and FeSOD than non-inoculated ones. Our results provide an evidence of the protective role of A. aculeatus in Perennial Ryegrass response to drought and heat stresses.
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Cadmium Tolerance of Perennial Ryegrass Induced by Aspergillus aculeatus
Frontiers in microbiology, 2018Co-Authors: Shijuan Han, Erick Amombo, Yan XieAbstract:Cadmium (Cd) pollution is becoming increasingly prevalent, posing a global environmental hazard due to its negative effects on plants growth and human health. Phytoremediation is a green technology that involves uptake of Cd from the soil by a combination of plants and associated microbes. The objective of this study was to investigate the role of Aspergillus aculeatus in Perennial Ryegrass Cd tolerance. This fungus produced indole-3-acetic acid, siderophores, and 1-aminocyclopropane-1-carboxylate deaminase. Physiological traits including growth rate, turf quality and chlorophyll content were measured to evaluate the physiological responses of Perennial Ryegrass to Cd stress. These physiological traits were improved after inoculated with A. aculeatus. Inoculation of A. aculeatus actively reduced DTPA-Cd concentration in the soil and Cd translocation to plant shoots. Chlorophyll a fluorescence transient and the C/N ratio in shoots were elevated by A. aculeatus, which implied that the fungus could protect the photosystem II against Cd stress and increase the photosynthetic efficiency. These results suggested that A. aculeatus is beneficial in improving Cd tolerance of Perennial Ryegrass and reducing Cd-induced injuries, thus, it has promising potential for application of phytostabilization in Cd contaminated soil.
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the fungus aspergillus aculeatus enhances salt stress tolerance metabolite accumulation and improves forage quality in Perennial Ryegrass
Frontiers in Microbiology, 2017Co-Authors: Shijuan Han, Erick Amombo, Guangyang Wang, Xiaoying Liu, Yan XieAbstract:Perennial Ryegrass (Lolium perenne) is an important forage grass with high yield and superior quality in temperate regions which is widely used in parks, sport field, and other places. However, Perennial Ryegrass is moderately tolerant to salinity stress compared to other commercial cultivars and salt stress reduces their growth and productivity. Aspergillus aculeatus has been documented to participate in alleviating damage induced by salinity. Therefore, the objective of this study was to investigate the mechanisms underlying A. aculeatus-mediated salt tolerance, and forage quality of Perennial Ryegrass exposed to 0, 200, and 400 mM NaCl concentrations. Physiological markers and forage quality of Perennial Ryegrass to salt stress were evaluated based on the growth rate, photosynthesis, antioxidant enzymes activity, lipid peroxidation, ionic homeostasis, the nutritional value of forage, and metabolites. Plants inoculated with A. aculeatus exhibited higher relative growth rate (RGR), turf and forage quality under salt stress than un-inoculated plants. Moreover, in inoculated plants, the fungus remarkably improved plant photosynthetic efficiency, reduced the antioxidant enzymes activity (POD and CAT), and attenuated lipid peroxidation (decreased H2O2 and MDA accumulation) induced by salinity, compared to un-inoculated plants. Furthermore, the fungus also acts as an important role in maintaining the lower Na/K ratio and metabolites and lower the amino acids (Alanine, Proline, GABA, and Asparagine), and soluble sugars (Glucose and Fructose) for inoculated plants than un-inoculated ones. Our results suggest that A. aculeatus may be involved in modulating Perennial Ryegrass tolerance to salinity in various ways.
Michael J Christensen - One of the best experts on this subject based on the ideXlab platform.
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an arbuscular mycorrhizal fungus and epichloe festucae var lolii reduce bipolaris sorokiniana disease incidence and improve Perennial Ryegrass growth
Mycorrhiza, 2018Co-Authors: Fang Li, Michael J Christensen, Yanzhong Li, Tingyu DuanAbstract:Leaf spot of Perennial Ryegrass (Lolium perenne) caused by Bipolaris sorokiniana is an important disease in temperate regions of the world. We designed this experiment to test for the combined effects of the arbuscular mycorrhizal (AM) fungus Claroideoglomus etunicatum and the grass endophyte fungus Epichloe festucae var. lolii on growth and disease occurrence in Perennial Ryegrass. The results show that C. etunicatum increased plant P uptake and total dry weight and that this beneficial effect was slightly enhanced when in association with the grass endophyte. The presence in plants of both the endophyte and B. sorokiniana decreased AM fungal colonization. Plants inoculated with B. sorokiniana showed the typical leaf spot symptoms 2 weeks after inoculation and the lowest disease incidence was with plants that were host to both C. etunicatum and E. festucae var. lolii. Plants with these two fungi had much higher activity of peroxidases (POD), superoxide dismutase (SOD) and catalase (CAT) and lower values of malondialdehyde (MDA) and hydrogen peroxide (H2O2). The AM fungus C. etunicatum and the grass endophyte fungus E. festucae var. lolii have the potential to promote Perennial Ryegrass growth and resistance to B. sorokiniana leaf spot.
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an arbuscular mycorrhizal fungus and epichloe festucae var lolii reduce bipolaris sorokiniana disease incidence and improve Perennial Ryegrass growth
Mycorrhiza, 2018Co-Authors: Yane Guo, Michael J Christensen, Ping Gao, Tingyu DuanAbstract:Leaf spot of Perennial Ryegrass (Lolium perenne) caused by Bipolaris sorokiniana is an important disease in temperate regions of the world. We designed this experiment to test for the combined effects of the arbuscular mycorrhizal (AM) fungus Claroideoglomus etunicatum and the grass endophyte fungus Epichloe festucae var. lolii on growth and disease occurrence in Perennial Ryegrass. The results show that C. etunicatum increased plant P uptake and total dry weight and that this beneficial effect was slightly enhanced when in association with the grass endophyte. The presence in plants of both the endophyte and B. sorokiniana decreased AM fungal colonization. Plants inoculated with B. sorokiniana showed the typical leaf spot symptoms 2 weeks after inoculation and the lowest disease incidence was with plants that were host to both C. etunicatum and E. festucae var. lolii. Plants with these two fungi had much higher activity of peroxidases (POD), superoxide dismutase (SOD) and catalase (CAT) and lower values of malondialdehyde (MDA) and hydrogen peroxide (H2O2). The AM fungus C. etunicatum and the grass endophyte fungus E. festucae var. lolii have the potential to promote Perennial Ryegrass growth and resistance to B. sorokiniana leaf spot.
Erick Amombo - One of the best experts on this subject based on the ideXlab platform.
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aspergillus aculeatus enhances potassium uptake and photosynthetic characteristics in Perennial Ryegrass by increasing potassium availability
Journal of Applied Microbiology, 2021Co-Authors: Yanling Yin, Erick Amombo, Shugao Fan, Yan XieAbstract:AIM Potassium (K) is a key determinant for plant development and productivity. However, more than 90% of K in the soil exists in an insoluble form. K-solubilizing microbes play an important role in the transformation of insoluble K. Thus, the objective of this study was to evaluate K-dissolving ability of Aspergillus aculeatus (F) and growth-promoting properties in Perennial Ryegrass. METHODS AND RESULTS Perennial Ryegrass inoculated with A. aculeatus exhibited enhanced soluble K accompanied with higher growth rate and turf quality, compared with the noninoculated regimen. In addition, A. aculeatus also played a primary role in increasing chlorophyll content and photosynthetic capacity of the plant exposed to LK+F (K-feldspar plus A. aculeatus) treatment, compared with the CK (control, no K-feldspar and A. aculeatus), F (only A. aculeatus) and LK (only K-feldspar) groups. Furthermore, the antioxidase activities (CAT and POD) were significantly increased while the oxidative damage (EL and MDA) was dramatically decreased in the LK+F group compared to the LK (K-feldspar) group. Finally, in Perennial Ryegrass leaves, the genes expression levels of HAK8, HAK12 and HKT18 were obviously elevated in the LK+F group, compared to the CK, F and LK groups. CONCLUSION We concluded that A. aculeatus could solubilize K from bound form and be considered as K-solubilizing biofertilizer through supplementing K in soil. SIGNIFICANCE AND IMPACT OF THE STUDY Aspergillus aculeatus has the potential to be used as a biofertilizer in sustainable agriculture.
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Exogenous Aspergillus aculeatus Enhances Drought and Heat Tolerance of Perennial Ryegrass.
Frontiers in microbiology, 2021Co-Authors: Chuncheng Zhao, Erick Amombo, Ting Zhang, Guangyang Wang, Yan XieAbstract:Perennial Ryegrass (Lolium perenne) is a cool-season grass whose growth and development are limited by drought and high temperature. Aspergillus aculeatus has been reported to promote plant growth and counteract the adverse effects of abiotic stresses. The objective of this study was to assess A. aculeatus-induced response mechanisms to drought and heat resistance in Perennial Ryegrass. We evaluated the physiological and biochemical markers of drought and heat stress based on the hormone homeostasis, photosynthesis, antioxidant enzymes activity, lipid peroxidation, and genes expression level. We found out that under drought and heat stress, A. aculeatus-inoculated leaves exhibited higher abscisic acid (ABA) and lower salicylic acid (SA) contents than non-inoculated regimes. In addition, under drought and heat stress, the fungus enhanced the photosynthetic performance, decreased the antioxidase activities, and mitigated membrane lipid peroxidation compared to non-inoculated regime. Furthermore, under drought stress, A. aculeatus induced a dramatic upregulation of sHSP17.8 and DREB1A and a downregulation of POD47, Cu/ZnSOD, and FeSOD genes. In addition, under heat stress, A. aculeatus-inoculated plants exhibited a higher expression level of HSP26.7a, sHSP17.8, and DREB1A while a lower expression level of POD47 and FeSOD than non-inoculated ones. Our results provide an evidence of the protective role of A. aculeatus in Perennial Ryegrass response to drought and heat stresses.
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Cadmium Tolerance of Perennial Ryegrass Induced by Aspergillus aculeatus
Frontiers in microbiology, 2018Co-Authors: Shijuan Han, Erick Amombo, Yan XieAbstract:Cadmium (Cd) pollution is becoming increasingly prevalent, posing a global environmental hazard due to its negative effects on plants growth and human health. Phytoremediation is a green technology that involves uptake of Cd from the soil by a combination of plants and associated microbes. The objective of this study was to investigate the role of Aspergillus aculeatus in Perennial Ryegrass Cd tolerance. This fungus produced indole-3-acetic acid, siderophores, and 1-aminocyclopropane-1-carboxylate deaminase. Physiological traits including growth rate, turf quality and chlorophyll content were measured to evaluate the physiological responses of Perennial Ryegrass to Cd stress. These physiological traits were improved after inoculated with A. aculeatus. Inoculation of A. aculeatus actively reduced DTPA-Cd concentration in the soil and Cd translocation to plant shoots. Chlorophyll a fluorescence transient and the C/N ratio in shoots were elevated by A. aculeatus, which implied that the fungus could protect the photosystem II against Cd stress and increase the photosynthetic efficiency. These results suggested that A. aculeatus is beneficial in improving Cd tolerance of Perennial Ryegrass and reducing Cd-induced injuries, thus, it has promising potential for application of phytostabilization in Cd contaminated soil.
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the fungus aspergillus aculeatus enhances salt stress tolerance metabolite accumulation and improves forage quality in Perennial Ryegrass
Frontiers in Microbiology, 2017Co-Authors: Shijuan Han, Erick Amombo, Guangyang Wang, Xiaoying Liu, Yan XieAbstract:Perennial Ryegrass (Lolium perenne) is an important forage grass with high yield and superior quality in temperate regions which is widely used in parks, sport field, and other places. However, Perennial Ryegrass is moderately tolerant to salinity stress compared to other commercial cultivars and salt stress reduces their growth and productivity. Aspergillus aculeatus has been documented to participate in alleviating damage induced by salinity. Therefore, the objective of this study was to investigate the mechanisms underlying A. aculeatus-mediated salt tolerance, and forage quality of Perennial Ryegrass exposed to 0, 200, and 400 mM NaCl concentrations. Physiological markers and forage quality of Perennial Ryegrass to salt stress were evaluated based on the growth rate, photosynthesis, antioxidant enzymes activity, lipid peroxidation, ionic homeostasis, the nutritional value of forage, and metabolites. Plants inoculated with A. aculeatus exhibited higher relative growth rate (RGR), turf and forage quality under salt stress than un-inoculated plants. Moreover, in inoculated plants, the fungus remarkably improved plant photosynthetic efficiency, reduced the antioxidant enzymes activity (POD and CAT), and attenuated lipid peroxidation (decreased H2O2 and MDA accumulation) induced by salinity, compared to un-inoculated plants. Furthermore, the fungus also acts as an important role in maintaining the lower Na/K ratio and metabolites and lower the amino acids (Alanine, Proline, GABA, and Asparagine), and soluble sugars (Glucose and Fructose) for inoculated plants than un-inoculated ones. Our results suggest that A. aculeatus may be involved in modulating Perennial Ryegrass tolerance to salinity in various ways.
W Uddin - One of the best experts on this subject based on the ideXlab platform.
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induced systemic resistance responses in Perennial Ryegrass against magnaporthe oryzae elicited by semi purified surfactin lipopeptides and live cells of bacillus amyloliquefaciens
Molecular Plant Pathology, 2015Co-Authors: Alamgir Rahman, W Uddin, Nancy G WennerAbstract:Summary The suppressive ability of several strains of cyclic lipopeptide-producing Bacillus rhizobacteria to grey leaf spot disease caused by Magnaporthe oryzae has been documented previously; however, the underlying mechanism(s) involved in the induced systemic resistance (ISR) activity in Perennial Ryegrass (Lolium perenne L.) remains unknown. Root-drench application of solid-phase extraction (SPE)-enriched surfactin and live cells of mutant Bacillus amyloliquefaciens strain FZB42-AK3 (produces surfactin, but not bacillomycin D and fengycin) significantly reduced disease incidence and severity on Perennial Ryegrass. The application of the treatments revealed a pronounced multilayered ISR defence response activation via timely and enhanced accumulation of hydrogen peroxide (H2O2), elevated cell wall/apoplastic peroxidase activity, and deposition of callose and phenolic/polyphenolic compounds underneath the fungal appressoria in naive leaves, which was significantly more intense in treated plants than in mock-treated controls. Moreover, a hypersensitive response (HR)-type reaction and enhanced expression of LpPrx (Prx, peroxidase), LpOXO4 (OXO, oxalate oxidase), LpPAL (PAL, phenylalanine ammonia lyase), LpLOXa (LOX, lipoxygenase), LpTHb (putative defensin) and LpDEFa (DEFa, putative defensin) in Perennial Ryegrass were associated with SPE-enriched surfactin and live AK3 cell treatments, acting as a second layer of defence when pre-invasive defence responses failed. The results indicate that ISR activity following surfactin perception may sensitize H2O2-mediated defence responses, thereby providing Perennial Ryegrass with enhanced protection against M. oryzae.
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silicon induced systemic defense responses in Perennial Ryegrass against infection by magnaporthe oryzae
Phytopathology, 2015Co-Authors: Alamgir Rahman, Christopher M Wallis, W UddinAbstract:Sustainable integrated disease management for gray leaf spot of Perennial Ryegrass may involve use of plant defense elicitors with compatible traditional fungicides to reduce disease incidence and severity. Silicon (Si) has previously been identified as a potential inducer or modulator of plant defenses against different fungal pathogens. To this end, Perennial Ryegrass was inoculated with the causal agent of gray leaf spot, Magnaporthe oryzae, when grown in soil that was nonamended or amended with three different levels of calcium silicate (1, 5, or 10 metric tons [t]/ha). When applied at a rate of 5 t/ha, calcium silicate was found to significantly suppress gray leaf spot in Perennial Ryegrass, including a significant reduction of disease incidence (39.5%) and disease severity (47.3%). Additional studies observed nonpenetrated papillae or cell-wall appositions harboring callose, phenolic autofluorogens, and lignin-associated polyphenolic compounds in grass grown in the Si-amended soil. Regarding defense...
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soil silicon amendment for managing gray leaf spot of Perennial Ryegrass turf on golf courses in pennsylvania
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie, 2009Co-Authors: U N Nanayakkara, W Uddin, Lawrence E DatnoffAbstract:Gray leaf spot (Magnaporthe oryzae) of Perennial Ryegrass (Lolium perenne) is commonly managed by fungicide applications. With increasing public concerns over chemical usage and emergence of fungicide-resistant pathogen populations, the golf course industry seeks disease management through integrated management practices. Silicon (Si) amendments have been proven effective in controlling fungal diseases of various crops including turfgrasses. This study investigated the effects of Si applications on gray leaf spot development on Perennial Ryegrass turf on commercial golf courses. Silicon sources wollastonite and calcium silicate slag were applied at 0, 0.5, 1, 2, 5, and 10 t/ha at two golf courses in Pennsylvania with different soil types: an Ultisol and an Alfisol. The turf was inoculated with M. oryzae 10 weeks after silicon application, and disease development was monitored over a 10 week period. Results showed that area under the disease progress curve and final disease severity 10 weeks after inoculat...
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pyricularia grisea causing gray leaf spot of Perennial Ryegrass turf population structure and host specificity
Plant Disease, 2001Co-Authors: G Viji, W Uddin, Seogchan Kang, David R HuffAbstract:ABSTRACT Gray leaf spot is a serious disease of Perennial Ryegrass (Lolium perenne) turf in the United States. Isolates of Pyricularia grisea causing the disease in Perennial Ryegrass were characterized using molecular markers and pathogenicity assays on various gramineous hosts. Genetic relationships among Perennial Ryegrass isolates were determined using different types of trans-posons as probes. Phylogenetic analysis using Pot2 and MGR586 probes, analyzed with AMOVA (analysis of molecular variance), showed that these isolates from Perennial Ryegrass consist of three closely related lineages. All the isolates belonged to a single mating type, MAT1-2. Among 20 isolates from 16 host species other than Perennial Ryegrass, only the isolates from wheat (Triticum aestivum) and triticale (× Triticosecale), showed notable similarity to the Perennial Ryegrass isolates based on their Pot2 fingerprints. The copy number and fingerprints of Pot2 and MGR586 in isolates of P. grisea from Perennial Ryegrass indicate th...
