The Experts below are selected from a list of 240 Experts worldwide ranked by ideXlab platform
José Luiz Guerra - One of the best experts on this subject based on the ideXlab platform.
-
Expression of Connexins 43, 26 and 32 in normal, hyperplastic and neoplasic Perianal dog Glands.
Brazilian Journal of Veterinary Pathology, 2010Co-Authors: Ana Maria Cristina Rabello Pinto Da Fonseca Martins, José Luiz Guerra, Sílvia Catarina Salgado Oloris, José Luis Avanzo, Cyntia Esteves De Lima, Maria Lúcia Zaidan DagliAbstract:Connexin (Cx) expression is reportedly altered in neoplasms. This study aimed to investigate the expression of Cx43, 26 and 32 in normal and pathological canine Perianal Glands. Thirty Perianal Glands bearing pathological processes and ten normal canine Perianal Glands were submitted to immunohistochemistry to search for presence of Cx43, Cx26 and Cx32. Both Cx43 and Cx26 expressions were observed in normal, hyperplastic Glands, and in well and moderately differentiated adenomas. However, in poorly differentiated adenomas, expressions were reduced, and they were absent in carcinomas. Cx26 was located in the cytoplasm of normal, hyperplastic Perianal Gland cells, and in well and moderately differentiated adenomas. Cx32 was not observed in any neoplasm neither in normal or hyperplastic Glands. Our results show that Cx43 and Cx26 expressions are altered in more aggressive canine Perianal Gland neoplasms, and we conclude that they may be related to the Perianal Gland carcinogenesis process.
-
Retrospective--systematic study and quantitative analysis of cellular proliferation and apoptosis in normal, hyperplastic and neoplastic Perianal Glands in dogs.
Veterinary and comparative oncology, 2008Co-Authors: Ana Maria Cristina Rabello Pinto Da Fonseca Martins, Maria Lúcia Zaidan Dagli, A Vasques-peyser, Luciana Neves Torres, Júlia Maria Matera, José Luiz GuerraAbstract:Neoplasms in the Perianal region are frequently diagnosed in dogs. The aetiology is unknown, and most of them are benign. In this study, 240 neoplasms of the Perianal Glands of dogs were retrieved from the Department of Pathology archives of the Faculty of Veterinary Medicine and Zootechny of University of Sao Paulo (FMVZ/USP), from 1984 to 2004. All 240 cases were re-examined by two pathologists. Nine cases (4%) were diagnosed as hyperplasia, 49 (20%) as group I adenoma, 81 (34%) were classified as moderately differentiated adenomas of the group II, 46 (19%) were poorly differentiated adenomas of group II, 48 (20%) were carcinoma of the group III according to the classification proposed by Berrocal, and 7 (13%) were other kind of tumours. Males over 8 years of age were predominantly affected. Cell proliferation was quantified by counting proliferating cell nuclear antigen (PCNA) positive nuclei, and apoptosis was quantified by counting fluorescent eosin-stained apoptotic corpuscles (AC) in normal tissue, hyperplasia and in different histologic types of neoplasia of these Glands. A parallel pattern of increase in both parameters (cell proliferation and apoptosis) was obtained. The net growth index (NGI), represents how much a cell population is proliferating or dying and was achieved by dividing the mean PCNA count in 1000 cells by the mean AC stain count in 1000 cells. NGI was different between hyperplasia and neoplasia; group I adenomas have a much higher potential of growth, and NGI decreases from benign towards malignant lesions. These results show up the importance of studying cell proliferation and apoptosis to understand the carcinogenesis of dog Perianal Gland.
D. Ivanyi - One of the best experts on this subject based on the ideXlab platform.
-
The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific Glands in the canine anal region.
Veterinary pathology, 1993Co-Authors: J. H. Vos, T.s.g.a.m. Van Den Ingh, R. F. Molenbeek, F. C. S. Ramaekers, M. De Neijs, F. N. Van Mil, D. IvanyiAbstract:Eight canine tumors originating from specific Glandular structures in the anal region, as well as metastatic tumor tissue of two of these cases (case Nos. 7, 8), were immunohistochemically analyzed using various monoclonal antibodies (MoAbs) directed against human keratin types, vimentin, neurofilament proteins, and alpha-smooth muscle actin. These tumors also were stained for the broad-spectrum neuroendocrine markers neuron-specific enolase (NSE) and synaptophysin. In histologically normal canine anal structures, alpha-smooth muscle actin and NSE antibodies stained basally localized (probably myoepithelial) cells in the anal Glands and the anal sac Glands. NSE staining also was present in a limited number of luminal cells in both anal Glands and anal sac Glands. Synaptophysin labeling was not observed in any of these Glandular structures. Histologically, the tumors were differentiated into well- and moderately differentiated Perianal Gland tumors (n = 5) and carcinomas without Perianal Gland differentiation (n = 3), corresponding to the so-called apocrine carcinomas of the anal region. Immunohistochemically, the Perianal Gland tumors could be differentiated from the carcinomas by marked differences in staining pattern with the various keratin MoAbs, particularly MoAbs directed against human keratin types 7 and 18. The keratin-staining characteristics of the carcinomas suggest a Glandular luminal cell origin. Metastases of the carcinomas showed loss of some keratin-staining characteristics as compared with the primary tumor. Staining for NSE was only observed in solitary cells and small cell clusters in the carcinomas and their metastases, whereas the alpha-smooth muscle actin antibody did not react with the carcinoma cells. None of the tumors stained for neurofilament proteins or synaptophysin. An unequivocal neuroendocrine nature of the carcinomas could not be substantiated by our immunohistochemical study, although the presence of a population of neuroendocrine cells within these neoplasms seems likely. Because the immunohistochemical features of the carcinomas with respect to various keratin MoAbs and NSE are similar to those of the anal Glands and the anal sac Glands, both these Glands might be considered as site of origin of these carcinomas.
-
Keratin and vimentin distribution patterns in the epithelial structures of the canine anal region.
Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology, 1992Co-Authors: T.s.g.a.m. Van Den Ingh, F. C. S. Ramaekers, M. De Neijs, D. IvanyiAbstract:The intermediate filament labeling pattern of the epithelial structures of the canine anal region was studied with different polypeptide specific keratin monoclonal antibodies (MoAbs) and with a monoclonal and polyclonal vimentin antibody. The epithelial structures in this region could be discriminated and characterized by differences in their keratin staining pattern. The basal cells in the different epithelial structures showed a similar staining pattern characterized by reactivity with MoAbs staining keratins 5, 8, 14, and 17. Columnar epithelial cells showed a completely different phenotype mostly characterized by reactivity with MoAbs staining keratins 7, 5, 8, 18, and 19. A restricted number of differentiated Perianal Gland cells showed perinuclear vimentin staining. Myoepithelial cells did not stain for vimentin, but, as other basal cells, were positive for MoAbs staining keratins 5, 8, 14, and 17.© Willey-Liss, Inc.
-
Keratin and vimentin distribution patterns in the epithelial structures of the canine anal region.
The Anatomical record, 1992Co-Authors: J. H. Vos, T.s.g.a.m. Van Den Ingh, F. C. S. Ramaekers, M. De Neijs, F. N. Van Mil, D. IvanyiAbstract:The intermediate filament labeling pattern of the epithelial structures of the canine anal region was studied with different polypeptide specific keratin monoclonal antibodies (MoAbs) and with a monoclonal and polyclonal vimentin antibody. The epithelial structures in this region could be discriminated and characterized by differences in their keratin staining pattern. The basal cells in the different epithelial structures showed a similar staining pattern characterized by reactivity with MoAbs staining keratins 5, 8, 14, and 17. Columnar epithelial cells showed a completely different phenotype mostly characterized by reactivity with MoAbs staining keratins 7, 5, 8, 18, and 19. A restricted number of differentiated Perianal Gland cells showed perinuclear vimentin staining. Myoepithelial cells did not stain for vimentin, but, as other basal cells, were positive for MoAbs staining keratins 5, 8, 14, and 17.
Ana Maria Cristina Rabello Pinto Da Fonseca Martins - One of the best experts on this subject based on the ideXlab platform.
-
Expression of Connexins 43, 26 and 32 in normal, hyperplastic and neoplasic Perianal dog Glands.
Brazilian Journal of Veterinary Pathology, 2010Co-Authors: Ana Maria Cristina Rabello Pinto Da Fonseca Martins, José Luiz Guerra, Sílvia Catarina Salgado Oloris, José Luis Avanzo, Cyntia Esteves De Lima, Maria Lúcia Zaidan DagliAbstract:Connexin (Cx) expression is reportedly altered in neoplasms. This study aimed to investigate the expression of Cx43, 26 and 32 in normal and pathological canine Perianal Glands. Thirty Perianal Glands bearing pathological processes and ten normal canine Perianal Glands were submitted to immunohistochemistry to search for presence of Cx43, Cx26 and Cx32. Both Cx43 and Cx26 expressions were observed in normal, hyperplastic Glands, and in well and moderately differentiated adenomas. However, in poorly differentiated adenomas, expressions were reduced, and they were absent in carcinomas. Cx26 was located in the cytoplasm of normal, hyperplastic Perianal Gland cells, and in well and moderately differentiated adenomas. Cx32 was not observed in any neoplasm neither in normal or hyperplastic Glands. Our results show that Cx43 and Cx26 expressions are altered in more aggressive canine Perianal Gland neoplasms, and we conclude that they may be related to the Perianal Gland carcinogenesis process.
-
Retrospective--systematic study and quantitative analysis of cellular proliferation and apoptosis in normal, hyperplastic and neoplastic Perianal Glands in dogs.
Veterinary and comparative oncology, 2008Co-Authors: Ana Maria Cristina Rabello Pinto Da Fonseca Martins, Maria Lúcia Zaidan Dagli, A Vasques-peyser, Luciana Neves Torres, Júlia Maria Matera, José Luiz GuerraAbstract:Neoplasms in the Perianal region are frequently diagnosed in dogs. The aetiology is unknown, and most of them are benign. In this study, 240 neoplasms of the Perianal Glands of dogs were retrieved from the Department of Pathology archives of the Faculty of Veterinary Medicine and Zootechny of University of Sao Paulo (FMVZ/USP), from 1984 to 2004. All 240 cases were re-examined by two pathologists. Nine cases (4%) were diagnosed as hyperplasia, 49 (20%) as group I adenoma, 81 (34%) were classified as moderately differentiated adenomas of the group II, 46 (19%) were poorly differentiated adenomas of group II, 48 (20%) were carcinoma of the group III according to the classification proposed by Berrocal, and 7 (13%) were other kind of tumours. Males over 8 years of age were predominantly affected. Cell proliferation was quantified by counting proliferating cell nuclear antigen (PCNA) positive nuclei, and apoptosis was quantified by counting fluorescent eosin-stained apoptotic corpuscles (AC) in normal tissue, hyperplasia and in different histologic types of neoplasia of these Glands. A parallel pattern of increase in both parameters (cell proliferation and apoptosis) was obtained. The net growth index (NGI), represents how much a cell population is proliferating or dying and was achieved by dividing the mean PCNA count in 1000 cells by the mean AC stain count in 1000 cells. NGI was different between hyperplasia and neoplasia; group I adenomas have a much higher potential of growth, and NGI decreases from benign towards malignant lesions. These results show up the importance of studying cell proliferation and apoptosis to understand the carcinogenesis of dog Perianal Gland.
-
Retrospective - systematic study and quantitative analysis of the cellular proliferation and apoptosis and identification of connexin 43 and aberrant 26 protein in normal, hyperplasic and neoplastic Perianal Glands in dogs
Universidade de São Paulo, 2006Co-Authors: Ana Maria Cristina Rabello Pinto Da Fonseca MartinsAbstract:Duzentos e quarenta e cinco neoplasias de glândula Perianal de cães dos arquivos do Departamento de Patologia da FMVZ/ USP, de 1984 à 2004, foram revisadas histologicamente. A grande maioria dos casos (34%) foi classificada como adenoma moderadamente diferenciado, grupo II, em machos com mais de oito anos de idade o que reflete a dependência androgênica dessas neoplasias. A análise quantitativa da proliferação celular e apoptose nos diferentes tipos histológicos de neoplasias, hiperplasia e tecido normal dessas glândulas determinou um padrão paralelo de aumento de ambas as quantificações. Com os resultados do índice de crescimento ajustado obtivemos que, embora os carcinomas tenham um nível de proliferação celular muito maior que os adenomas (grupo I), esses têm um potencial de crescimento maior, levando-se em conta a apoptose. Investigamos, também, a expressão de Cx43, 26 e 32 nessas glândulas perianais com métodos imunoistoquímicos. A Cx 43 expressava-se em glândulas perianais normais, hiperplásicas, adenomas (grupo I) e adenomas moderadamente diferenciados. Nos adenomas pouco diferenciados (grupo II), a expressão estava reduzida e não se expressava nos carcinomas (grupo III). A Cx 26 acumulava-se no citoplasma nas glândulas normais, hiperplásicas, adenomas (grupo I) e adenomas moderadamente diferenciados (grupo II). Nos adenomas pouco diferenciados (grupo II), a expressão estava reduzida e, ausente, nos carcinomas. A Cx 32 não foi identificada em nenhum dos grupos (I, II e III) ou glândulas normais e hiperplásicas. Concluindo, Cx 43 e Cx26 são importantes para a homeostasia de glândula Perianal normal, podendo estar associadas aos receptores de andrógenos presentes em suas células. Este foi o primeiro estudo mostrando a apoptose e sua influência na fase promocional da carcinogênese e, também, o primeiro estudo mostrando a expressão de Cx43, Cx 26 citoplasmática e ausência de expressão da Cx 32 em glândulas perianais normais, hiperplásicas e neoplásicas em cãesTwo hundred and forty five neoplasms of the Perianal Glands of dogs from the archives of the Department of Pathology of the FMVZ/USP, 1984 to 2004, have been reviewed hystologically. Most of the cases (34%) were classified as moderately differentiated adenomas, group II, in males over 8 years of age, which showed an androgenic dependence of these neoplasms. The quantitative analysis of the cellular proliferation and apoptosis, in the different hystologic types of neoplasia, hyperplasia and normal tissue of these Glands, determined a parallel pattern of increase in both quantifications. The values of the net growth index showed that although carcinomas have a much higher level of proliferation than adenomas (group I), these latter have a much higher potential of growth, taking into account the effect of the apoptosis. The occurrence of Cx 43, 26 and 32 in these Perianal Glands was also investigated by immunohystochemical methods. Cx 43 expression was present in normal, hyperplasic, adenomas (group I) and moderately differentiated adenomas Perianal Glands. In poorly differentiated adenomas (group II), the expression was reduced and was absent in carcinomas. Cx 26 was accumulated in the cytoplasm in normal, hyperplasic, adenomas (group I) and moderately differentiated adenomas Glands. In poorly differentiated adenomas (group II), the expression was reduced and was absent in carcinomas. Cx 32 was not found in all groups (I, II, III), normal and hyperplasic Glands. In conclusion, Cx 43 and Cx 26 are important for homeostasis of normal canine Perianal Glands, being able to be associated with the androgen receptors present in their cells. This was the first study showing the apoptosis and its influence on the promotional phase of carcinogenesis and, also, the first study showing the occurrence of Cx 43, cytoplasmatic Cx 26 and no expression of Cx 32 in normal, hyperplasic and neoplastic canine Perianal Gland
Adam Brodzki - One of the best experts on this subject based on the ideXlab platform.
-
Androgen and Estrogen Receptor Expression in Different Types of Perianal Gland Tumors in Male Dogs.
Animals : an open access journal from MDPI, 2021Co-Authors: Adam Brodzki, Wojciech Łopuszyński, Y. Millán, Marcin R. Tatara, Piotr Brodzki, Katarzyna Kulpa, Natalia MinakowAbstract:Perianal Gland tumors are modified sebaceous Glands present in the skin of the Perianal region in the dog. Hormonal stimulation may induce hyperplasia of the Perianal Glands or their neoplastic progression. The presence of androgen (AR) and estrogen (ER) receptors have been demonstrated both in normal Perianal Glands as well as in Perianal tumors. The aim of the study was an immunohistochemical assessment of the expression of estrogen and androgen receptors in Perianal Gland tumors in dogs as an applicatory marker for antihormonal treatment. Biopsy samples of Perianal masses were collected from 41 male dogs. A histopathological examination revealed 24 adenomas, 12 epitheliomas and five carcinomas. The immunohistochemical staining showed a mainly nuclear expression of AR and ER in the neoplastic cells. Both the androgen and estrogen receptors were expressed in adenoma, epithelioma and carcinoma cases; however, the highest expression of the receptors was stated in the adenoma and epithelioma. In the case of the carcinoma, the expression of sex hormone receptors was very weak. The differences of the number of cells expressing AR and ER as well as the observed differentiated intensity of staining in the studies demonstrated that the determination of the expression of the sex hormone receptors may be useful to elaborate a diagnostic and therapeutic algorithm.
-
dna adduct assessment during antihormonal treatment of Perianal Gland tumors with tamoxifen in male dogs
in Vivo, 2019Co-Authors: Adam Brodzki, Marcin R. Tatara, P Brodzki, Ireneusz BalickiAbstract:BACKGROUND/AIM Determination of DNA adduct count was performed in mononuclear cells during antihormonal treatment of Perianal Gland tumors. MATERIALS AND METHODS Eight- to fifteen-year-old dogs with carcinoma (CAR Group; N=5), epithelioma (EPI Group; N=16) or adenoma (ADE Group; N=24) were used. The control group suffered from perineal hernia or rectal diverticulum (CTR Group; N=25). Blood was collected at baseline, and at one and six months after the beginning of the anti-hormonal treatment with tamoxifen (1 mg/kg of body weight). DNA adduct count was determined using autoradiography. RESULTS At baseline, DNA adduct count reached the highest value in the CTR Group, and the lowest in the EPI Group (p<0.05). Six-month-long therapy with tamoxifen resulted in a significant increase in the DNA adduct count by 78.7%, 221.5% and 198.3% in the ADE, EPI and CAR groups, respectively (p<0.05). CONCLUSION Increased DNA adduct formation after long-term administration of tamoxifen shows its genotoxicity.
-
DNA Adduct Assessment During Antihormonal Treatment of Perianal Gland Tumors With Tamoxifen in Male Dogs.
In vivo (Athens Greece), 2019Co-Authors: Adam Brodzki, Marcin R. Tatara, Piotr Brodzki, Ireneusz BalickiAbstract:BACKGROUND/AIM Determination of DNA adduct count was performed in mononuclear cells during antihormonal treatment of Perianal Gland tumors. MATERIALS AND METHODS Eight- to fifteen-year-old dogs with carcinoma (CAR Group; N=5), epithelioma (EPI Group; N=16) or adenoma (ADE Group; N=24) were used. The control group suffered from perineal hernia or rectal diverticulum (CTR Group; N=25). Blood was collected at baseline, and at one and six months after the beginning of the anti-hormonal treatment with tamoxifen (1 mg/kg of body weight). DNA adduct count was determined using autoradiography. RESULTS At baseline, DNA adduct count reached the highest value in the CTR Group, and the lowest in the EPI Group (p
-
EGF Level in Hepatoid Gland Adenomas and Hepatoid Gland Epitheliomas in Dogs After Administering Tamoxifen.
In vivo (Athens Greece), 2018Co-Authors: Aleksandra Sobczyńska-rak, Adam Brodzki, Beata Żylińska, Łukasz Jarosz, Marcin R. TataraAbstract:BACKGROUND/AIM Neoplastic lesions of Perianal Glands account for approximately 10% of all skin cancer cases in dogs. They occur in many dog breeds, usually in male animals aged over 6 years. Due to their hormone-dependency, tamoxifen can be used in antineoplastic treatment. The aim of the study was to measure epidermal growth factor (EGF) levels in the serum of dogs with Perianal tumours after tamoxifen treatment and to use it as a prognostic factor for further treatment. MATERIALS AND METHODS The study was performed on 19 male dogs aged between 6 and 14 years, diagnosed with neoplastic hyperplasia in the Perianal region. The control group comprised 10 healthy dogs brought in for routine castration. The research material comprised blood drawn from the animals and tumour specimens for histopathology. The study group received 1-month treatment with tamoxifen. Blood serum was then tested for 17-β oestradiol level, and for EGF level on the first day of the therapy and 6 months after treatment completion. RESULTS Hepatoid Gland adenomas were diagnosed in 10 cases, and hepatoid Gland epitheliomas in nine cases. Elevated 17-β oestradiol levels were observed in all dogs. On the first day of treatment with tamoxifen, the serum EGF levels in all study groups were higher than in the control group. At the 6-month follow-up, the EGF levels were significantly reduced in hepatoid Gland adenoma cases compared to those taken on the first day of treatment of tamoxifen, while in animals with hepatoid Gland epithelioma, it was greatly increased and was correlated with relapse. CONCLUSION Perianal Gland tumours are characterised by EGF overexpression, which can be helpful in early-stage prognosis and treatment. An increase in EGF levels 6 months after tamoxifen therapy correlates with disease progression and may be a useful prognostic factor.
-
Diagnostic and prognostic value of cellular proliferation assessment with Ki-67 protein in dogs suffering from benign and malignant Perianal tumors.
Folia biologica, 2014Co-Authors: Adam Brodzki, Wojciech Łopuszyński, Piotr Brodzki, Marcin R. TataraAbstract:In the Perianal region of carnivores, skin consists of modified sebaceous Glands called Perianal Glands. Tumors originating from Perianal Glands are the third most frequent type of neoplasm in male dogs after neoplastic diseases of testes and skin. Ki-67 is a nuclear non-histone protein considered a proliferation marker in normal and neoplastic proliferating cells. Previous investigations revealed that Ki-67 expression may be used as a prognostic factor for breast cancer in humans. Thus, the aim of this study was to estimate the diagnostic and prognostic value of Ki-67 evaluation in dogs suffering from benign and malignant Perianal tumors. The highest value of the Ki-67 index was obtained in the carcinoma group (18.50% ± 2.68), significantly higher compared to the values obtained in the control tissue (7.63% ± 2.12) and adenoma (7.33% ± 1.06; all P < 0.05). Statistically significant differences in the Ki-67 index were not found between the epithelioma group (11.95% ± 1.96) and all other groups (P < 0.05). This investigation on dogs with Perianal Gland tumors has shown significantly increased expression of Ki-67 antigen in carcinoma cells, while the expression of this protein was similar in the case of control tissues, adenoma and epithelioma. Thus, it may be postulated that Ki-67 evaluation in Perianal Gland tumors in dogs may serve as a useful marker possessing high diagnostic and prognostic value and enabling differentiation of malignant and benign tumors.
Marcin R. Tatara - One of the best experts on this subject based on the ideXlab platform.
-
Androgen and Estrogen Receptor Expression in Different Types of Perianal Gland Tumors in Male Dogs.
Animals : an open access journal from MDPI, 2021Co-Authors: Adam Brodzki, Wojciech Łopuszyński, Y. Millán, Marcin R. Tatara, Piotr Brodzki, Katarzyna Kulpa, Natalia MinakowAbstract:Perianal Gland tumors are modified sebaceous Glands present in the skin of the Perianal region in the dog. Hormonal stimulation may induce hyperplasia of the Perianal Glands or their neoplastic progression. The presence of androgen (AR) and estrogen (ER) receptors have been demonstrated both in normal Perianal Glands as well as in Perianal tumors. The aim of the study was an immunohistochemical assessment of the expression of estrogen and androgen receptors in Perianal Gland tumors in dogs as an applicatory marker for antihormonal treatment. Biopsy samples of Perianal masses were collected from 41 male dogs. A histopathological examination revealed 24 adenomas, 12 epitheliomas and five carcinomas. The immunohistochemical staining showed a mainly nuclear expression of AR and ER in the neoplastic cells. Both the androgen and estrogen receptors were expressed in adenoma, epithelioma and carcinoma cases; however, the highest expression of the receptors was stated in the adenoma and epithelioma. In the case of the carcinoma, the expression of sex hormone receptors was very weak. The differences of the number of cells expressing AR and ER as well as the observed differentiated intensity of staining in the studies demonstrated that the determination of the expression of the sex hormone receptors may be useful to elaborate a diagnostic and therapeutic algorithm.
-
dna adduct assessment during antihormonal treatment of Perianal Gland tumors with tamoxifen in male dogs
in Vivo, 2019Co-Authors: Adam Brodzki, Marcin R. Tatara, P Brodzki, Ireneusz BalickiAbstract:BACKGROUND/AIM Determination of DNA adduct count was performed in mononuclear cells during antihormonal treatment of Perianal Gland tumors. MATERIALS AND METHODS Eight- to fifteen-year-old dogs with carcinoma (CAR Group; N=5), epithelioma (EPI Group; N=16) or adenoma (ADE Group; N=24) were used. The control group suffered from perineal hernia or rectal diverticulum (CTR Group; N=25). Blood was collected at baseline, and at one and six months after the beginning of the anti-hormonal treatment with tamoxifen (1 mg/kg of body weight). DNA adduct count was determined using autoradiography. RESULTS At baseline, DNA adduct count reached the highest value in the CTR Group, and the lowest in the EPI Group (p<0.05). Six-month-long therapy with tamoxifen resulted in a significant increase in the DNA adduct count by 78.7%, 221.5% and 198.3% in the ADE, EPI and CAR groups, respectively (p<0.05). CONCLUSION Increased DNA adduct formation after long-term administration of tamoxifen shows its genotoxicity.
-
DNA Adduct Assessment During Antihormonal Treatment of Perianal Gland Tumors With Tamoxifen in Male Dogs.
In vivo (Athens Greece), 2019Co-Authors: Adam Brodzki, Marcin R. Tatara, Piotr Brodzki, Ireneusz BalickiAbstract:BACKGROUND/AIM Determination of DNA adduct count was performed in mononuclear cells during antihormonal treatment of Perianal Gland tumors. MATERIALS AND METHODS Eight- to fifteen-year-old dogs with carcinoma (CAR Group; N=5), epithelioma (EPI Group; N=16) or adenoma (ADE Group; N=24) were used. The control group suffered from perineal hernia or rectal diverticulum (CTR Group; N=25). Blood was collected at baseline, and at one and six months after the beginning of the anti-hormonal treatment with tamoxifen (1 mg/kg of body weight). DNA adduct count was determined using autoradiography. RESULTS At baseline, DNA adduct count reached the highest value in the CTR Group, and the lowest in the EPI Group (p
-
EGF Level in Hepatoid Gland Adenomas and Hepatoid Gland Epitheliomas in Dogs After Administering Tamoxifen.
In vivo (Athens Greece), 2018Co-Authors: Aleksandra Sobczyńska-rak, Adam Brodzki, Beata Żylińska, Łukasz Jarosz, Marcin R. TataraAbstract:BACKGROUND/AIM Neoplastic lesions of Perianal Glands account for approximately 10% of all skin cancer cases in dogs. They occur in many dog breeds, usually in male animals aged over 6 years. Due to their hormone-dependency, tamoxifen can be used in antineoplastic treatment. The aim of the study was to measure epidermal growth factor (EGF) levels in the serum of dogs with Perianal tumours after tamoxifen treatment and to use it as a prognostic factor for further treatment. MATERIALS AND METHODS The study was performed on 19 male dogs aged between 6 and 14 years, diagnosed with neoplastic hyperplasia in the Perianal region. The control group comprised 10 healthy dogs brought in for routine castration. The research material comprised blood drawn from the animals and tumour specimens for histopathology. The study group received 1-month treatment with tamoxifen. Blood serum was then tested for 17-β oestradiol level, and for EGF level on the first day of the therapy and 6 months after treatment completion. RESULTS Hepatoid Gland adenomas were diagnosed in 10 cases, and hepatoid Gland epitheliomas in nine cases. Elevated 17-β oestradiol levels were observed in all dogs. On the first day of treatment with tamoxifen, the serum EGF levels in all study groups were higher than in the control group. At the 6-month follow-up, the EGF levels were significantly reduced in hepatoid Gland adenoma cases compared to those taken on the first day of treatment of tamoxifen, while in animals with hepatoid Gland epithelioma, it was greatly increased and was correlated with relapse. CONCLUSION Perianal Gland tumours are characterised by EGF overexpression, which can be helpful in early-stage prognosis and treatment. An increase in EGF levels 6 months after tamoxifen therapy correlates with disease progression and may be a useful prognostic factor.
-
Diagnostic and prognostic value of cellular proliferation assessment with Ki-67 protein in dogs suffering from benign and malignant Perianal tumors.
Folia biologica, 2014Co-Authors: Adam Brodzki, Wojciech Łopuszyński, Piotr Brodzki, Marcin R. TataraAbstract:In the Perianal region of carnivores, skin consists of modified sebaceous Glands called Perianal Glands. Tumors originating from Perianal Glands are the third most frequent type of neoplasm in male dogs after neoplastic diseases of testes and skin. Ki-67 is a nuclear non-histone protein considered a proliferation marker in normal and neoplastic proliferating cells. Previous investigations revealed that Ki-67 expression may be used as a prognostic factor for breast cancer in humans. Thus, the aim of this study was to estimate the diagnostic and prognostic value of Ki-67 evaluation in dogs suffering from benign and malignant Perianal tumors. The highest value of the Ki-67 index was obtained in the carcinoma group (18.50% ± 2.68), significantly higher compared to the values obtained in the control tissue (7.63% ± 2.12) and adenoma (7.33% ± 1.06; all P < 0.05). Statistically significant differences in the Ki-67 index were not found between the epithelioma group (11.95% ± 1.96) and all other groups (P < 0.05). This investigation on dogs with Perianal Gland tumors has shown significantly increased expression of Ki-67 antigen in carcinoma cells, while the expression of this protein was similar in the case of control tissues, adenoma and epithelioma. Thus, it may be postulated that Ki-67 evaluation in Perianal Gland tumors in dogs may serve as a useful marker possessing high diagnostic and prognostic value and enabling differentiation of malignant and benign tumors.