The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform
Shunji Natori - One of the best experts on this subject based on the ideXlab platform.
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Presence of the Periplaneta lectin-related protein family in the american cockroach Periplaneta americana
Insect biochemistry and molecular biology, 1996Co-Authors: Kiyoshi Kawasaki, Takeo Kubo, Shunji NatoriAbstract:We determined the partial amino acid sequences of Periplaneta lectin, which we had purified and characterized previously from the hemolymph of the American cockroach (Periplaneta americana) [Kubo T. and Natori S. (1987) Eur. J. Biochem. 168, 75–82]. Based on these sequences, we performed PCR and found that the cDNA library of the Periplaneta fat body contained many similar, but not identical, Periplaneta lectin-related cDNAs. Analysis of the cloned cDNAs suggested that Periplaneta has a protein family, of which the Periplaneta lectin and LPS binding protein purified previously are members.
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A novel role of Periplaneta lectin as an opsonin to recognize 2-keto-3-deoxy octonate residues of bacterial lipopolysaccharides.
Comparative biochemistry and physiology. B Comparative biochemistry, 1993Co-Authors: Kiyoshi Kawasaki, Takeo Kubo, Shunji NatoriAbstract:1. Periplaneta lectin of the American cockroach was shown to have affinity to bacterial lipopolysaccharides (LPS) of chemotypes Rb-Re, but not to lipid A. 2. 2-Keto-3-deoxy octonate (KDO) was the most effective sugar so far tested for inhibition of the hemagglutinating activity of Periplaneta lectin. 3. Periplaneta lectin was suggested to participate in the clearance of bacteria injected into the abdominal cavity of the cockroach.
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Transient Appearance and Localization of a 26-kDa Lectin, a Novel Member of the Periplaneta Lectin Family, in Regenerating Cockroach Leg
Developmental biology, 1993Co-Authors: Takeo Kubo, Kiyoshi Kawasaki, Shunji NatoriAbstract:A lectin consisting of a 26-kDa subunit was purified from the hemolymph of adult American cockroaches (Periplaneta americana) and shown to be a member of the Periplaneta lectin family. Immunoblotting analysis revealed that this lectin appeared transiently in regenerating legs after nymphal legs were amputated. Thus, its mode of appearance was similar to that of regenectin, another member of the Periplaneta lectin family. However, its time of appearance and localization in the regenerates were different from those of regenectin: the 26-kDa lectin appeared in regenerates in the middle stage of regeneration and was localized in the vicinity of the outside of newly formed epidermal cells. These results suggest that this lectin participates in the organization or stabilization of the epidermis during leg regeneration.
Kiyoshi Kawasaki - One of the best experts on this subject based on the ideXlab platform.
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Presence of the Periplaneta lectin-related protein family in the american cockroach Periplaneta americana
Insect biochemistry and molecular biology, 1996Co-Authors: Kiyoshi Kawasaki, Takeo Kubo, Shunji NatoriAbstract:We determined the partial amino acid sequences of Periplaneta lectin, which we had purified and characterized previously from the hemolymph of the American cockroach (Periplaneta americana) [Kubo T. and Natori S. (1987) Eur. J. Biochem. 168, 75–82]. Based on these sequences, we performed PCR and found that the cDNA library of the Periplaneta fat body contained many similar, but not identical, Periplaneta lectin-related cDNAs. Analysis of the cloned cDNAs suggested that Periplaneta has a protein family, of which the Periplaneta lectin and LPS binding protein purified previously are members.
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A novel role of Periplaneta lectin as an opsonin to recognize 2-keto-3-deoxy octonate residues of bacterial lipopolysaccharides.
Comparative biochemistry and physiology. B Comparative biochemistry, 1993Co-Authors: Kiyoshi Kawasaki, Takeo Kubo, Shunji NatoriAbstract:1. Periplaneta lectin of the American cockroach was shown to have affinity to bacterial lipopolysaccharides (LPS) of chemotypes Rb-Re, but not to lipid A. 2. 2-Keto-3-deoxy octonate (KDO) was the most effective sugar so far tested for inhibition of the hemagglutinating activity of Periplaneta lectin. 3. Periplaneta lectin was suggested to participate in the clearance of bacteria injected into the abdominal cavity of the cockroach.
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Transient Appearance and Localization of a 26-kDa Lectin, a Novel Member of the Periplaneta Lectin Family, in Regenerating Cockroach Leg
Developmental biology, 1993Co-Authors: Takeo Kubo, Kiyoshi Kawasaki, Shunji NatoriAbstract:A lectin consisting of a 26-kDa subunit was purified from the hemolymph of adult American cockroaches (Periplaneta americana) and shown to be a member of the Periplaneta lectin family. Immunoblotting analysis revealed that this lectin appeared transiently in regenerating legs after nymphal legs were amputated. Thus, its mode of appearance was similar to that of regenectin, another member of the Periplaneta lectin family. However, its time of appearance and localization in the regenerates were different from those of regenectin: the 26-kDa lectin appeared in regenerates in the middle stage of regeneration and was localized in the vicinity of the outside of newly formed epidermal cells. These results suggest that this lectin participates in the organization or stabilization of the epidermis during leg regeneration.
Takeo Kubo - One of the best experts on this subject based on the ideXlab platform.
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Presence of the Periplaneta lectin-related protein family in the american cockroach Periplaneta americana
Insect biochemistry and molecular biology, 1996Co-Authors: Kiyoshi Kawasaki, Takeo Kubo, Shunji NatoriAbstract:We determined the partial amino acid sequences of Periplaneta lectin, which we had purified and characterized previously from the hemolymph of the American cockroach (Periplaneta americana) [Kubo T. and Natori S. (1987) Eur. J. Biochem. 168, 75–82]. Based on these sequences, we performed PCR and found that the cDNA library of the Periplaneta fat body contained many similar, but not identical, Periplaneta lectin-related cDNAs. Analysis of the cloned cDNAs suggested that Periplaneta has a protein family, of which the Periplaneta lectin and LPS binding protein purified previously are members.
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A novel role of Periplaneta lectin as an opsonin to recognize 2-keto-3-deoxy octonate residues of bacterial lipopolysaccharides.
Comparative biochemistry and physiology. B Comparative biochemistry, 1993Co-Authors: Kiyoshi Kawasaki, Takeo Kubo, Shunji NatoriAbstract:1. Periplaneta lectin of the American cockroach was shown to have affinity to bacterial lipopolysaccharides (LPS) of chemotypes Rb-Re, but not to lipid A. 2. 2-Keto-3-deoxy octonate (KDO) was the most effective sugar so far tested for inhibition of the hemagglutinating activity of Periplaneta lectin. 3. Periplaneta lectin was suggested to participate in the clearance of bacteria injected into the abdominal cavity of the cockroach.
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Transient Appearance and Localization of a 26-kDa Lectin, a Novel Member of the Periplaneta Lectin Family, in Regenerating Cockroach Leg
Developmental biology, 1993Co-Authors: Takeo Kubo, Kiyoshi Kawasaki, Shunji NatoriAbstract:A lectin consisting of a 26-kDa subunit was purified from the hemolymph of adult American cockroaches (Periplaneta americana) and shown to be a member of the Periplaneta lectin family. Immunoblotting analysis revealed that this lectin appeared transiently in regenerating legs after nymphal legs were amputated. Thus, its mode of appearance was similar to that of regenectin, another member of the Periplaneta lectin family. However, its time of appearance and localization in the regenerates were different from those of regenectin: the 26-kDa lectin appeared in regenerates in the middle stage of regeneration and was localized in the vicinity of the outside of newly formed epidermal cells. These results suggest that this lectin participates in the organization or stabilization of the epidermis during leg regeneration.
Vilca Arcela, Carol Marily - One of the best experts on this subject based on the ideXlab platform.
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Periplaneta americana y la minimización de los residuos sólidos orgánicos en el distrito de Pucusana, 2019
'Universidad Cesar Vallejo', 2019Co-Authors: Vilca Arcela, Carol MarilyAbstract:El objetivo general de esta investigación fue determinar la eficiencia en la reducción de los residuos sólidos orgánicos mediante el empleo de Periplaneta americana en el distrito de Pucusana. El diseño de la investigación fue de tipo aplicada, enfoque cuantitativo, nivel explotario y de diseño experimental con pre y post prueba. La muestra de residuos sólidos orgánicos fue obtenida de un total de 11.75 toneladas/ día de residuos domiciliarios que se generan en dicho distrito, de donde se tomó 10 kg para realizar el proceso experimental. El desarrollo de la investigación consistió en depositar en un ambiente cerrado 10 kg de residuos sólidos orgánicos y allí se colocaron 150 Periplaneta americana, para luego monitorear la cantidad de disminución de residuos. Al cabo de 7 días se logró determinar que el empleo de Periplaneta americana redujo de 6.705Kg de 10.322 Kg inicial de residuos sólidos orgánicos. Las condiciones en el ambiente donde se realizó la investigación que para que la Periplaneta americana se alimente de los residuos sólidos orgánicos es, una humedad promedio de 25.7 °C y una temperatura promedio de 69.6%. Se pudo establecer que el empleo de la Periplaneta americana, resulta un proceso eficaz en la solución de la problemática de los residuos sólidos orgánicos, es viable y a la vez rentable y sostenible por cuanto existe la posibilidad de generar alimento balanceado por su importante contenido proteico con la consecuente utilidad ambiental y económica. Palabras claves: eficiencia, Periplaneta americana, residuos sólidos orgánicos
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Periplaneta americana y la minimización de los residuos sólidos orgánicos en el distrito de Pucusana, 2019
'Universidad Cesar Vallejo', 2019Co-Authors: Vilca Arcela, Carol MarilyAbstract:TesisLima NorteEscuela Académico Profesional de Ingeniería AmbientalTratamiento y Gestión de los ResiduosEl objetivo general de esta investigación fue determinar la eficiencia en la reducción de los residuos sólidos orgánicos mediante el empleo de Periplaneta americana en el distrito de Pucusana. El diseño de la investigación fue de tipo aplicada, enfoque cuantitativo, nivel explotario y de diseño experimental con pre y post prueba. La muestra de residuos sólidos orgánicos fue obtenida de un total de 11.75 toneladas/ día de residuos domiciliarios que se generan en dicho distrito, de donde se tomó 10 kg para realizar el proceso experimental. El desarrollo de la investigación consistió en depositar en un ambiente cerrado 10 kg de residuos sólidos orgánicos y allí se colocaron 150 Periplaneta americana, para luego monitorear la cantidad de disminución de residuos. Al cabo de 7 días se logró determinar que el empleo de Periplaneta americana redujo de 6.705Kg de 10.322 Kg inicial de residuos sólidos orgánicos. Las condiciones en el ambiente donde se realizó la investigación que para que la Periplaneta americana se alimente de los residuos sólidos orgánicos es, una humedad promedio de 25.7 °C y una temperatura promedio de 69.6%. Se pudo establecer que el empleo de la Periplaneta americana, resulta un proceso eficaz en la solución de la problemática de los residuos sólidos orgánicos, es viable y a la vez rentable y sostenible por cuanto existe la posibilidad de generar alimento balanceado por su importante contenido proteico con la consecuente utilidad ambiental y económica. Palabras claves: eficiencia, Periplaneta americana, residuos sólidos orgánicos
Christine C Meade - One of the best experts on this subject based on the ideXlab platform.
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transplantation immunity in the american cockroach Periplaneta americana the rejection of integumentary grafts from blatta orientalis
Developmental and Comparative Immunology, 1993Co-Authors: Richard D Karp, Christine C MeadeAbstract:Abstract The results from several previous studies have indicated that the American cockroach is able to respond to integumentary xenografts, but doubt remained as to whether cockroaches could effectively discriminate between self and allogeneic differences. This was emphasized by the fact that while one study reported that Periplaneta americana responded to grafts donated by the closely related genus Blatta orientalis , the data reported elsewhere, using a different assay system, found no such reactivity. Since we have subsequently reported, using a direct histological assay, that Periplaneta can in fact recognize and destroy integumentary allografts, we initiated a study to hopefully sort out the enigma presented by previous data using Blatta as a transplant donor. Integument from Blatta orientalis was transplanted orthotopically onto Periplaneta americana , and at various time points post-transplant, scored histologically for the survival of the subcuticular epidermal layer. The results clearly demonstrated that Periplaneta reacted to the Blatta tissue, because approximately 82% of the grafts had the epidermal layer destroyed by day 7 posttransplant. The kinetics of the response to Blatta was more in line with our allograft data, which would be in agreement with other work indicating that the closer the donor and recipient are on the phylogenetic tree, the less intense the reactivity to the foreign transplant.