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Rafael De Prado - One of the best experts on this subject based on the ideXlab platform.
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from tolerance to resistance mechanisms governing the differential response to glyphosate in chloris barbata
Pest Management Science, 2018Co-Authors: Enzo Bracamonte, Ricardo Alcantarade La Cruz, Hugo Cruzhipolito, Jose A Dominguezvalenzuela, Hellen Martins Da Silveira, Rafael De PradoAbstract:BACKGROUND Susceptibility and the mechanism (s) governing tolerance/resistance to glyphosate were characterized in two putative-glyphosate-resistant Chloris barbata populations (R1 and R2), collected in Persian Lime orchards from Colima State, Mexico, comparing them with one non-treated population (referred to as S). RESULTS Glyphosate doses required to reduce fresh weight or cause mortality by 50% were 4.2–6.4 times higher in resistant populations than in the S population. The S population accumulated 4.3 and 5.2 times more shikimate than the R2 and R1 populations, respectively. There were no differences in 14C-glyphosate uptake between R and S populations, but the R plants translocated at least 12% less herbicide to the rest of plant and roots 96 h after treatment. Insignificant amounts of glyphosate were metabolized to aminomethyl phosphonate and glyoxylate in both R and S plants. The 5-enolpyruvylshikimate-3-phosphate synthase gene of the R populations contained the Pro106-Ser mutation, giving them a resistance 12 (R2) and 14.7 (R1) times greater at target-site level compared with the S population. CONCLUSION The Pro106-Ser mutation governs the resistance to glyphosate of the R1 and R2 C barbata populations, but the impaired translocation could contribute to the resistance. These results confirm the first case of glyphosate resistance evolved in this species. © 2018 Society of Chemical Industry
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pro 106 ser mutation and epsps overexpression acting together simultaneously in glyphosate resistant goosegrass eleusine indica
Scientific Reports, 2017Co-Authors: Javid Gherekhloo, Pablo T Fernandezmoreno, Ricardo Alcantarade La Cruz, Eduardo Sanchezgonzalez, Hugo Cruzhipolito, Jose A Dominguezvalenzuela, Rafael De PradoAbstract:Glyphosate has been used for more than 15 years for weed management in citrus groves in the Gulf of Mexico, at up to 3–4 applications per year. Goosegrass (Eleusine indica (L.) Gaertn.) control has sometimes failed. In this research, the mechanisms governing three goosegrass biotypes (Ein-Or from an orange grove, and Ein-Pl1 and Ein-Pl2 from Persian Lime groves) with suspected resistance to glyphosate were characterized and compared to a susceptible biotype (Ein-S). Dose-response and shikimate accumulation assays confirmed resistance of the resistant (R) biotypes. There were no differences in glyphosate absorption, but the R biotypes retained up to 62–78% of the herbicide in the treated leaf at 96 h after treatment (HAT), in comparison to the Ein-S biotype (36%). The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity in the Ein-Or and Ein-S biotypes was over 100-fold lower than the Ein-Pl1 and Ein-Pl2 ones. The latter showed a high EPSPS-basal activity, a mutation at Pro-106-Ser position in the EPSPS gene, and EPSPS overexpression. The EPSPS basal and EPSPS overexpression were positively correlated. The R goosegrass biotypes displayed poor glyphosate translocation. Furthermore, this grassweed showed, for the first time, two mechanisms at the target-site level (Pro-106-Ser mutation + EPSPS overexpression) acting together simultaneously against glyphosate.
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Genetic Relationships between Tropical Sprangletop (Leptochloa virgata) Populations from Mexico: Understanding Glyphosate Resistance Spread
Weed Science, 2016Co-Authors: Ricardo Alcantarade La Cruz, José A. Domínguez-valenzuela, Yolanda Romano, María Dolores Osuna-ruiz, Julio Menéndez, Rafael De PradoAbstract:The susceptibility to glyphosate and genetic diversity based on intersimple sequence repeat markers were characterized for 17 tropical sprangletop populations collected from two separate regions mainly in Persian Lime groves in Veracruz, Mexico. The whole-plant dose response together with shikimic acid assays indicated different levels of glyphosate resistance in those populations. Genetic diversity values (h) estimated using POPGENE ranged from 0.119 to 0.198 and 0.117 to 0.214 within susceptible and resistant populations, respectively. The average genetic diversity (HS) within the susceptible populations was 0.157, and the total genetic diversity (HT) was 0.218. The HS of the resistant populations was 0.144, and the HT was 0.186. The analysis of molecular variance based on the response to glyphosate indicated that most of the genetic variation was found within groups of susceptible and resistant populations (90% of the genetic variation), whereas 10% or less was among groups. The high level of genetic d...
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Characterization of Glyphosate-Resistant Tropical Sprangletop (Leptochloa virgata) and Its Alternative Chemical Control in Persian Lime Orchards in Mexico
Weed Science, 2014Co-Authors: Macrina Pérez-lópez, Fidel González-torralva, Hugo Cruz-hipolito, Francisco Santos, José A. Domínguez-valenzuela, Rafael De PradoAbstract:Abstract Field, greenhouse, and laboratory experiments were conducted to investigate resistance to glyphosate in tropical sprangletop biotypes (Lv8 and Lv9) collected in Persian Lime from Veracruz, Mexico. Assays to determine the dose required to reduce seedling fresh weight by 50% indicated a resistance factor (RF) of 4.9 and 3.2 for biotypes Lv8 and Lv9, respectively; whereas the LD50 showed a RF of 4.4 and 3.3 for biotypes Lv8 and Lv9, respectively. On the other hand, the RFs using whole plant dose–response assays were lower (RF of 3 for Lv8 and 2.3 for Lv9). The susceptible biotype (LvS) accumulated 5.5 and 11.8 times more shikimate than biotypes Lv8 and Lv9, respectively, at 96 h after treatment (HAT). In field experiments, alternatives to glyphosate-resistant tropical sprangletop management were identified. Indaziflam + glufosinate and paraquat + diuron provided over 80% control of in-field populations of tropical sprangletop at 60 d after treatment (DAT). These results confirmed the first reported ...
Montserrat Calderón-santoyo - One of the best experts on this subject based on the ideXlab platform.
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USE OF A MARINE YEAST AS A BIOCONTROL AGENT OF THE NOVEL PATHOGEN PENICILLIUM CITRINUM ON Persian Lime
Emirates Journal of Food and Agriculture, 2017Co-Authors: Ramss Gonzlezestrada, Felipe Ascencio-valle, Juan Ragazzosnchez, Montserrat Calderón-santoyoAbstract:Penicillium citrinum was isolated from fruits of a packinghouse in San Pedro Lagunillas, Nayarit, Mexico and turned out to be an endemic pathogen against Persian Lime. This is the first report that involves this strain as a pathogen for citrus. The yeast Debaryomyces hansenii was evaluated for its effectiveness against P. citrinum in Persian Lime. Dextrose and Tween 80 were added to the D. hansenii suspension in order to enhance its establishment in the fruit carposphere. The control of Persian Lime decay caused by P. citrinum in postharvest and yeast population dynamics were evaluated. A 100% decay inhibition was obtained by the sole application of Debaryomyces hansenii even if population counts were minimal (3.3x105 CFU/fruit) at the end of the storage period (25th day) at 25°C and 60-75% of relative humidity. On the other hand, treatments with dextrose and Tween 80 promoted a better establishment of the yeast (3.2x108 UFC/fruit - 25 days storage), but infection by P. citrinum on Persian Lime was higher (20 to 60% of infected fruits).
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Antimicrobial soy protein based coatings: Application to Persian Lime ( Citrus latifolia Tanaka) for protection and preservation
Postharvest Biology and Technology, 2017Co-Authors: Ramsés Ramón González-estrada, Pascale Chalier, Juan Arturo Ragazzo-sánchez, Dilara Konuk, Montserrat Calderón-santoyoAbstract:Preventive antifungal activity of postharvest treatments with SPI (soy protein isolate) −coatings forming solutions against blue mould decay were evaluated on Persian Lime (Citrus latifolia Tanaka) artificially inoculated in rind wounds with Penicillium italicum. Stimulatory effects were observed with the use of citral, however with the use of limonene an inhibitory effect was obtained. After 13 d of storage, significant preventive activity against blue mould was observed with a 20% of disease incidence applying SPI-coating forming solutions with limonene added. SEM micrographs showed that limonene could act as an inhibitor of germ tube elongation, delaying the process of infection. Quality parameters like water losses, change in colour and aroma compounds release were assessed on fruit coated. Depending to storage condition, SPI-coating forming solutions were effective to reduce water losses, maintain colour and controlling the liberation of active agent.
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Control of blue mold decay on Persian Lime: Application of covalently cross-linked arabinoxylans bioactive coatings with antagonistic yeast entrapped
LWT - Food Science and Technology, 2017Co-Authors: Ramsés Ramón González-estrada, Juan Arturo Ragazzo-sánchez, Elizabeth Carvajal-millan, Pedro Ulises Bautista-rosales, Montserrat Calderón-santoyoAbstract:Abstract A novel edible film and coating with antagonistic yeast in arabinoxylan (AX) matrix was prepared and its effects on preserving against fungus infection on Limes were investigated. The effect of yeast cell incorporation of developed films was evaluated in terms of viability, biocontrol activity and barrier properties. Furthermore, the application of AX as bioactive coatings was investigated in order to evaluate their efficacy to preserve postharvest quality and prevent or cure infection by Penicillium italicum during storage. Results revealed AX as a matrix compatible with D. hansenii and able to maintain more than 97% viability of the initial inoculum at temperatures tested, films with antagonistic yeast are capable to inhibit the growth of P. italicum in vitro. Presence of yeast did not change water vapor permeability on films. Viability of yeasts was preserved on coated Limes on 100%. Preventive application of treatments was more effective than curative applications in the control of blue mold decay. Yeast added coatings were effective at reducing weight loss and had no effect on Limes color. This study demonstrates the potential application of bioactive AX coatings with D. hansenii as an alternative of postharvest disease management.
Ricardo Alcantarade La Cruz - One of the best experts on this subject based on the ideXlab platform.
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from tolerance to resistance mechanisms governing the differential response to glyphosate in chloris barbata
Pest Management Science, 2018Co-Authors: Enzo Bracamonte, Ricardo Alcantarade La Cruz, Hugo Cruzhipolito, Jose A Dominguezvalenzuela, Hellen Martins Da Silveira, Rafael De PradoAbstract:BACKGROUND Susceptibility and the mechanism (s) governing tolerance/resistance to glyphosate were characterized in two putative-glyphosate-resistant Chloris barbata populations (R1 and R2), collected in Persian Lime orchards from Colima State, Mexico, comparing them with one non-treated population (referred to as S). RESULTS Glyphosate doses required to reduce fresh weight or cause mortality by 50% were 4.2–6.4 times higher in resistant populations than in the S population. The S population accumulated 4.3 and 5.2 times more shikimate than the R2 and R1 populations, respectively. There were no differences in 14C-glyphosate uptake between R and S populations, but the R plants translocated at least 12% less herbicide to the rest of plant and roots 96 h after treatment. Insignificant amounts of glyphosate were metabolized to aminomethyl phosphonate and glyoxylate in both R and S plants. The 5-enolpyruvylshikimate-3-phosphate synthase gene of the R populations contained the Pro106-Ser mutation, giving them a resistance 12 (R2) and 14.7 (R1) times greater at target-site level compared with the S population. CONCLUSION The Pro106-Ser mutation governs the resistance to glyphosate of the R1 and R2 C barbata populations, but the impaired translocation could contribute to the resistance. These results confirm the first case of glyphosate resistance evolved in this species. © 2018 Society of Chemical Industry
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pro 106 ser mutation and epsps overexpression acting together simultaneously in glyphosate resistant goosegrass eleusine indica
Scientific Reports, 2017Co-Authors: Javid Gherekhloo, Pablo T Fernandezmoreno, Ricardo Alcantarade La Cruz, Eduardo Sanchezgonzalez, Hugo Cruzhipolito, Jose A Dominguezvalenzuela, Rafael De PradoAbstract:Glyphosate has been used for more than 15 years for weed management in citrus groves in the Gulf of Mexico, at up to 3–4 applications per year. Goosegrass (Eleusine indica (L.) Gaertn.) control has sometimes failed. In this research, the mechanisms governing three goosegrass biotypes (Ein-Or from an orange grove, and Ein-Pl1 and Ein-Pl2 from Persian Lime groves) with suspected resistance to glyphosate were characterized and compared to a susceptible biotype (Ein-S). Dose-response and shikimate accumulation assays confirmed resistance of the resistant (R) biotypes. There were no differences in glyphosate absorption, but the R biotypes retained up to 62–78% of the herbicide in the treated leaf at 96 h after treatment (HAT), in comparison to the Ein-S biotype (36%). The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity in the Ein-Or and Ein-S biotypes was over 100-fold lower than the Ein-Pl1 and Ein-Pl2 ones. The latter showed a high EPSPS-basal activity, a mutation at Pro-106-Ser position in the EPSPS gene, and EPSPS overexpression. The EPSPS basal and EPSPS overexpression were positively correlated. The R goosegrass biotypes displayed poor glyphosate translocation. Furthermore, this grassweed showed, for the first time, two mechanisms at the target-site level (Pro-106-Ser mutation + EPSPS overexpression) acting together simultaneously against glyphosate.
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Genetic Relationships between Tropical Sprangletop (Leptochloa virgata) Populations from Mexico: Understanding Glyphosate Resistance Spread
Weed Science, 2016Co-Authors: Ricardo Alcantarade La Cruz, José A. Domínguez-valenzuela, Yolanda Romano, María Dolores Osuna-ruiz, Julio Menéndez, Rafael De PradoAbstract:The susceptibility to glyphosate and genetic diversity based on intersimple sequence repeat markers were characterized for 17 tropical sprangletop populations collected from two separate regions mainly in Persian Lime groves in Veracruz, Mexico. The whole-plant dose response together with shikimic acid assays indicated different levels of glyphosate resistance in those populations. Genetic diversity values (h) estimated using POPGENE ranged from 0.119 to 0.198 and 0.117 to 0.214 within susceptible and resistant populations, respectively. The average genetic diversity (HS) within the susceptible populations was 0.157, and the total genetic diversity (HT) was 0.218. The HS of the resistant populations was 0.144, and the HT was 0.186. The analysis of molecular variance based on the response to glyphosate indicated that most of the genetic variation was found within groups of susceptible and resistant populations (90% of the genetic variation), whereas 10% or less was among groups. The high level of genetic d...
Mendoza Peña Estrella - One of the best experts on this subject based on the ideXlab platform.
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Compuestos volátiles de brotes de limón persa y limón mexicano asociados a síntomas del HLB (Huanglongbing).
Colegio de Postgraduados (COLPOS), 2014Co-Authors: Mendoza Peña EstrellaAbstract:El Huanglongbing (HLB) es una enfermedad de los cítricos que se caracteriza por causar áreas moteadas en hojas, generar frutos deformes y decolorados, semillas abortivas, causar pérdida paulatina de la producción y provocar que la muerte de los árboles infectados sea inevitable. El agente asociado a esta enfermedad es la bacteria Candidatus Liberibacter ssp., y en México es dispersada por el vector Diaphorina citri Kuwayama (Hemiptera: Liviidae). En el presente estudio, mediante el método de microextracción en fase solida (SPME) se recolectaron compuestos volátiles emitidos de brotes vegetativos (menores a 3 cm) sanos, así como de brotes infectados con Ca. L. asiaticus, de limón persa (Citrus latifolia Tanaka) y limón mexicano (Citrus aurantifolia Christm). Los compuestos capturados se analizaron por cromatografía de gases acoplada a un detector selectivo de masas (CG-DSM). Se identificaron 41 compuestos de los cuales 34 estuvieron presentes en C. latifolia y 16 de ellos en C. aurantifolia. La información analizada mostró que ambas especies de cítricos ácidos presentan estadísticamente similitud en el número de compuestos volátiles emitidos por brotes de árboles asintomáticos y sintomáticos; también se encontró la presencia constante de tres compuestos en todas las muestras, los cuales fueron D-limoneno, β-ocimeno y cariofileno; estos difirieron cuantitativamente entre los recolectados de brotes de árboles asintomáticos y los obtenidos de árboles con síntomas característicos de HLB, encontrándose mayor concentración de D-limoneno y β-ocimeno en brotes de árboles sintomáticos. El análisis también demostró que compuestos como el citronellal, lavadulol, β-elemeno, α-terpineol para limón persa y elemeno para limón mexicano tienden a disminuir su concentración cuando los árboles presentan síntomas de HLB. Los resultados obtenidos presentan implicaciones que podrían contribuir en el desarrollo del diagnóstico temprano de la enfermedad. _______________ ABSTRACT: The Huanglongbing (HLB) is a citrus disease characterized by areas causing mottled leaves, deformed and discolored bear fruit, seed abortion, causing gradual loss of production and cause the death of infected trees is inevitable. The causal agent is Candidatus Liberibacter spp. in Mexico is scattered by the vector Diaphorina citri Kuwayama (Hemiptera: Liviidae). In this paper, we used the solid phase microextraction (SPME) technique for collecting volatiles from vegetative shoots (less than 3 cm) healthy and infecteds prouts by Candidatus Liberibacter asiaticus from Persian Lime (Citrus latifolia Tanaka) and Mexican Lime (Citrus aurantifolia Christm). Captured compounds were analyzed by gas chromatography coupled to mass selective detector (GC-DSM). We were able to identify 41compounds, 16were present in C. aurantifolia and 34 in C. latifolia. The analysis indicated that no significant difference in the number of volatile compounds emitted by shoots of asymptomatic and symptomatic trees in both cultivars. Three compounds occurred in all samples, D-limonene, β-ocimene and caryophyllene and these differed quantitatively between leave buds collected from symptomless trees and leave shoots collected with characteristic symptoms of HLB, being highest concentration in shoots from symptomatic trees. The analysis also showed that compounds such as citronellal, lavadulol, β-elemene, α-terpineol for Persian Lime and Mexican Lime elemene tend to decrease their concentration when the trees show symptoms of HLB. The results have implications that could contribute to the development of early diagnosis of the disease
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Compuestos volátiles de brotes de limón persa y limón mexicano asociados a síntomas del HLB (Huanglongbing).
Colegio de Postgraduados (COLPOS), 2013Co-Authors: Mendoza Peña EstrellaAbstract:El Huanglongbing (HLB) es una enfermedad de los cítricos que se caracteriza por causar áreas moteadas en hojas, generar frutos deformes y decolorados, semillas abortivas, causar pérdida paulatina de la producción y provocar que la muerte de los árboles infectados sea inevitable. El agente asociado a esta enfermedad es la bacteria Candidatus Liberibacter ssp., y en México es dispersada por el vector Diaphorina citri Kuwayama (Hemiptera: Liviidae). En el presente estudio, mediante el método de microextracción en fase solida (SPME) se recolectaron compuestos volátiles emitidos de brotes vegetativos (menores a 3 cm) sanos, así como de brotes infectados con Ca. L. asiaticus, de limón persa (Citrus latifolia Tanaka) y limón mexicano (Citrus aurantifolia Christm). Los compuestos capturados se analizaron por cromatografía de gases acoplada a un detector selectivo de masas (CG-DSM). Se identificaron 41 compuestos de los cuales 34 estuvieron presentes en C. latifolia y 16 de ellos en C. aurantifolia. La información analizada mostró que ambas especies de cítricos ácidos presentan estadísticamente similitud en el número de compuestos volátiles emitidos por brotes de árboles asintomáticos y sintomáticos; también se encontró la presencia constante de tres compuestos en todas las muestras, los cuales fueron D-limoneno, β-ocimeno y cariofileno; estos difirieron cuantitativamente entre los recolectados de brotes de árboles asintomáticos y los obtenidos de árboles con síntomas característicos de HLB, encontrándose mayor concentración de D-limoneno y β-ocimeno en brotes de árboles sintomáticos. El análisis también demostró que compuestos como el citronellal, lavadulol, β-elemeno, α-terpineol para limón persa y elemeno para limón mexicano tienden a disminuir su concentración cuando los árboles presentan síntomas de HLB. Los resultados obtenidos presentan implicaciones que podrían contribuir en el desarrollo del diagnóstico temprano de la enfermedad. _______________ ABSTRACT: The Huanglongbing (HLB) is a citrus disease characterized by areas causing mottled leaves, deformed and discolored bear fruit, seed abortion, causing gradual loss of production and cause the death of infected trees is inevitable. The causal agent is Candidatus Liberibacter spp. in Mexico is scattered by the vector Diaphorina citri Kuwayama (Hemiptera: Liviidae). In this paper, we used the solid phase microextraction (SPME) technique for collecting volatiles from vegetative shoots (less than 3 cm) healthy and infecteds prouts by Candidatus Liberibacter asiaticus from Persian Lime (Citrus latifolia Tanaka) and Mexican Lime (Citrus aurantifolia Christm). Captured compounds were analyzed by gas chromatography coupled to mass selective detector (GC-DSM). We were able to identify 41compounds, 16were present in C. aurantifolia and 34 in C. latifolia. The analysis indicated that no significant difference in the number of volatile compounds emitted by shoots of asymptomatic and symptomatic trees in both cultivars. Three compounds occurred in all samples, D-limonene, β-ocimene and caryophyllene and these differed quantitatively between leave buds collected from symptomless trees and leave shoots collected with characteristic symptoms of HLB, being highest concentration in shoots from symptomatic trees. The analysis also showed that compounds such as citronellal, lavadulol, β-elemene, α-terpineol for Persian Lime and Mexican Lime elemene tend to decrease their concentration when the trees show symptoms of HLB. The results have implications that could contribute to the development of early diagnosis of the disease.Tesis (Maestría en Ciencias, especialista en Entomología y Acarología).- Colegio de Postgraduados, 2013.Consejo Nacional de Ciencia y Tecnología (CONACYT)
Ramsés Ramón González-estrada - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial soy protein based coatings: Application to Persian Lime ( Citrus latifolia Tanaka) for protection and preservation
Postharvest Biology and Technology, 2017Co-Authors: Ramsés Ramón González-estrada, Pascale Chalier, Juan Arturo Ragazzo-sánchez, Dilara Konuk, Montserrat Calderón-santoyoAbstract:Preventive antifungal activity of postharvest treatments with SPI (soy protein isolate) −coatings forming solutions against blue mould decay were evaluated on Persian Lime (Citrus latifolia Tanaka) artificially inoculated in rind wounds with Penicillium italicum. Stimulatory effects were observed with the use of citral, however with the use of limonene an inhibitory effect was obtained. After 13 d of storage, significant preventive activity against blue mould was observed with a 20% of disease incidence applying SPI-coating forming solutions with limonene added. SEM micrographs showed that limonene could act as an inhibitor of germ tube elongation, delaying the process of infection. Quality parameters like water losses, change in colour and aroma compounds release were assessed on fruit coated. Depending to storage condition, SPI-coating forming solutions were effective to reduce water losses, maintain colour and controlling the liberation of active agent.
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Control of blue mold decay on Persian Lime: Application of covalently cross-linked arabinoxylans bioactive coatings with antagonistic yeast entrapped
LWT - Food Science and Technology, 2017Co-Authors: Ramsés Ramón González-estrada, Juan Arturo Ragazzo-sánchez, Elizabeth Carvajal-millan, Pedro Ulises Bautista-rosales, Montserrat Calderón-santoyoAbstract:Abstract A novel edible film and coating with antagonistic yeast in arabinoxylan (AX) matrix was prepared and its effects on preserving against fungus infection on Limes were investigated. The effect of yeast cell incorporation of developed films was evaluated in terms of viability, biocontrol activity and barrier properties. Furthermore, the application of AX as bioactive coatings was investigated in order to evaluate their efficacy to preserve postharvest quality and prevent or cure infection by Penicillium italicum during storage. Results revealed AX as a matrix compatible with D. hansenii and able to maintain more than 97% viability of the initial inoculum at temperatures tested, films with antagonistic yeast are capable to inhibit the growth of P. italicum in vitro. Presence of yeast did not change water vapor permeability on films. Viability of yeasts was preserved on coated Limes on 100%. Preventive application of treatments was more effective than curative applications in the control of blue mold decay. Yeast added coatings were effective at reducing weight loss and had no effect on Limes color. This study demonstrates the potential application of bioactive AX coatings with D. hansenii as an alternative of postharvest disease management.