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Marco Salvatore - One of the best experts on this subject based on the ideXlab platform.

  • Multislice CT with single-Phase Technique in patients with suspected pancreatic cancer.
    La Radiologia medica, 2006
    Co-Authors: Massimo Imbriaco, D. Smeraldo, Raffaele Liuzzi, F. Carrillo, G. Cacace, D. Vecchione, A. Diglio, Marco Salvatore
    Abstract:

    Purpose. The purpose of this study was to evaluate the role of multislice computed tomography (MSCT) with a single–Phase Technique in patients with suspected pancreatic cancer (PC).

  • Multislice CT with single–Phase Technique in patients with suspected pancreatic cancer
    La radiologia medica, 2006
    Co-Authors: Massimo Imbriaco, D. Smeraldo, Raffaele Liuzzi, F. Carrillo, G. Cacace, D. Vecchione, A. Diglio, Marco Salvatore
    Abstract:

    Purpose. The purpose of this study was to evaluate the role of multislice computed tomography (MSCT) with a single–Phase Technique in patients with suspected pancreatic cancer (PC). Materials and methods. Seventy–eight patients underwent MSCT with the following technical parameters: collimation: 4x1 mm; pitch 1; 120 kVp; 260 mAs. The pre–contrast scan was followed by a single acquisition Phase in the caudocranial direction from the inferior hepatic margin to the diaphragm with a 60–s delay after IV administration of 150 ml of iodinated contrast material at a rate of 3 ml/s. Two radiologists assessed the single images independently. Receiver operating characteristics (ROC) curves were obtained for each of the two observers. Results. The final diagnosis was pancreatic cancer in 46 cases and chronic pancreatitis in 32 cases. Areas under the curve (A_z) for diagnosis and evaluation of disease resectability were 0.97 and 0.93 for the first observer ( p =ns), and 0.97 and 0.90 for the second observer ( p =ns). The mean difference in tissue attenuation values between the cancer and normal pancreas was 72±3 Hounsfield units (HU). No statistically significant differences were observed in the degree of opacification between the peripancreatic arteries and veins. Conclusions. MSCT with a single–Phase Technique is an accurate and reproducible method for diagnosis and evaluation of disease resectability in patients with suspected PC, ensuring optimal tumour–to–pancreas contrast and maximal opacification of the main peripancreatic arterial and venous structures.

  • Value of the single-Phase Technique in MDCT assessment of pancreatic tumors.
    AJR. American journal of roentgenology, 2005
    Co-Authors: Massimo Imbriaco, Raffaele Liuzzi, Alec J. Megibow, Alfonso Ragozzino, P.p. Mainenti, Sara Bortone, Luigi Camera, Marco Salvatore
    Abstract:

    OBJECTIVE. The purpose of our study was to determine the diagnostic value of single-Phase MDCT in patients with suspected pancreatic carcinoma.SUBJECTS AND METHODS. Seventy-one patients (41 men, 30 women; mean age, 63 years; range, 29-80 years) with suspected pancreatic tumor underwent MDCT. Scanning was performed on an MDCT scanner with 0.5-sec gantry rotation and acquisition of 4 slices per rotation. Unenhanced scanning was followed by one set of scanning in the caudocranial direction from the inferior hepatic margin to the diaphragm with a scanning delay of 60 sec after the IV injection of 150 mL of contrast material delivered at 3 mL/sec. Two reviewers independently scored images in a blinded fashion for the presence of tumor and assessment of resectability. Receiver operating characteristic analysis was performed.RESULTS. A final histopathologic diagnosis derived from surgical findings was obtained in 42 patients; in the remaining 29 patients, percutaneous fine-needle aspiration biopsy coupled with a...

Massimo Imbriaco - One of the best experts on this subject based on the ideXlab platform.

  • Multislice CT with single-Phase Technique in patients with suspected pancreatic cancer.
    La Radiologia medica, 2006
    Co-Authors: Massimo Imbriaco, D. Smeraldo, Raffaele Liuzzi, F. Carrillo, G. Cacace, D. Vecchione, A. Diglio, Marco Salvatore
    Abstract:

    Purpose. The purpose of this study was to evaluate the role of multislice computed tomography (MSCT) with a single–Phase Technique in patients with suspected pancreatic cancer (PC).

  • Multislice CT with single–Phase Technique in patients with suspected pancreatic cancer
    La radiologia medica, 2006
    Co-Authors: Massimo Imbriaco, D. Smeraldo, Raffaele Liuzzi, F. Carrillo, G. Cacace, D. Vecchione, A. Diglio, Marco Salvatore
    Abstract:

    Purpose. The purpose of this study was to evaluate the role of multislice computed tomography (MSCT) with a single–Phase Technique in patients with suspected pancreatic cancer (PC). Materials and methods. Seventy–eight patients underwent MSCT with the following technical parameters: collimation: 4x1 mm; pitch 1; 120 kVp; 260 mAs. The pre–contrast scan was followed by a single acquisition Phase in the caudocranial direction from the inferior hepatic margin to the diaphragm with a 60–s delay after IV administration of 150 ml of iodinated contrast material at a rate of 3 ml/s. Two radiologists assessed the single images independently. Receiver operating characteristics (ROC) curves were obtained for each of the two observers. Results. The final diagnosis was pancreatic cancer in 46 cases and chronic pancreatitis in 32 cases. Areas under the curve (A_z) for diagnosis and evaluation of disease resectability were 0.97 and 0.93 for the first observer ( p =ns), and 0.97 and 0.90 for the second observer ( p =ns). The mean difference in tissue attenuation values between the cancer and normal pancreas was 72±3 Hounsfield units (HU). No statistically significant differences were observed in the degree of opacification between the peripancreatic arteries and veins. Conclusions. MSCT with a single–Phase Technique is an accurate and reproducible method for diagnosis and evaluation of disease resectability in patients with suspected PC, ensuring optimal tumour–to–pancreas contrast and maximal opacification of the main peripancreatic arterial and venous structures.

  • Value of the single-Phase Technique in MDCT assessment of pancreatic tumors.
    AJR. American journal of roentgenology, 2005
    Co-Authors: Massimo Imbriaco, Raffaele Liuzzi, Alec J. Megibow, Alfonso Ragozzino, P.p. Mainenti, Sara Bortone, Luigi Camera, Marco Salvatore
    Abstract:

    OBJECTIVE. The purpose of our study was to determine the diagnostic value of single-Phase MDCT in patients with suspected pancreatic carcinoma.SUBJECTS AND METHODS. Seventy-one patients (41 men, 30 women; mean age, 63 years; range, 29-80 years) with suspected pancreatic tumor underwent MDCT. Scanning was performed on an MDCT scanner with 0.5-sec gantry rotation and acquisition of 4 slices per rotation. Unenhanced scanning was followed by one set of scanning in the caudocranial direction from the inferior hepatic margin to the diaphragm with a scanning delay of 60 sec after the IV injection of 150 mL of contrast material delivered at 3 mL/sec. Two reviewers independently scored images in a blinded fashion for the presence of tumor and assessment of resectability. Receiver operating characteristic analysis was performed.RESULTS. A final histopathologic diagnosis derived from surgical findings was obtained in 42 patients; in the remaining 29 patients, percutaneous fine-needle aspiration biopsy coupled with a...

Nobutaka Fujii - One of the best experts on this subject based on the ideXlab platform.

  • Affinity purification method using a reversible biotinylating reagent for peptides synthesized by the solid-Phase Technique
    Journal of Chromatography A, 1993
    Co-Authors: Susumu Funakoshi, Hiroyuki Fukuda, Nobutaka Fujii
    Abstract:

    Abstract The specific interaction between biotin and avidin was explored as the basis of an affinity purification procedure for peptides synthesized by the solid-Phase Technique. In this affinity purification procedure, a reversible biotinylating reagent, 2-[(N-biotinyl)-aminoethylsulphonyl]ethyl p -nitrophenyl carbonate (BASEC), was synthesized. Using this reagent, the procedure involves the following sequence of four reactions: (i) attachment of the biotin to the N-terminus of the peptide-resin through a base-labile sulphonylethoxycarbonyl linkage at the final step of solid-Phase peptide synthesis; (ii) acid treatment to remove side-chain protecting groups and cleave the biotin-modified peptide from the resin; (iii) affinity purification of the biotinyl-peptide on an avidin-agarose colunm; and (iv) base (5% ammonia solution) treatment to remove the biotin moiety from the peptide. To facilitate this purification procedure, unreacted amino groups were acetylated in each step during solid-Phase synthesis. The usefulness of this method was demonstrated by the purification of several peptides synthesized by the 9-fluorenylmethoxycarbonyl (Fmoc)-based solid-Phase Technique.

  • Chemoselective one-step purification method for peptides synthesized by the solid-Phase Technique.
    Proceedings of the National Academy of Sciences of the United States of America, 1991
    Co-Authors: Susumu Funakoshi, Hiroyuki Fukuda, Nobutaka Fujii
    Abstract:

    Abstract The specific reaction between SH and iodoacetamide groups has been explored as the basis of an affinity-type purification procedure for peptides synthesized by the solid-Phase Technique. For this affinity-type purification procedure, we synthesized an SH precursor reagent bearing an acid-labile S-protecting group, pMB-SCH2CONHCH2CH2-SO2CH2CH2OCO2pNP (compound I), in which pMB is p-methoxybenzyl and pNP is p-nitrophenyl. Using this reagent, the procedure involves the following sequence of four reactions: (i) attachment of the SH function of compound I to the alpha-amino group of a peptide-resin through a base-labile sulfonylethoxycarbonyl linkage in the final step of solid-Phase peptide synthesis, (ii) acid treatment to remove the S-pMB and side-chain-protecting groups employed and cleave the modified peptide from the resin, (iii) immobilization of the derived SH-peptide on an iodoacetamide-resin column, and (iv) base (5% NH4OH) treatment to release the desired peptide from the resin in nearly pure form. To facilitate this purification procedure, unreacted amino groups were acetylated in each step during solid-Phase synthesis. The usefulness of this method was demonstrated by the purification of several peptides (18 to approximately 44 amino acids in length) synthesized by the 9-fluorenylmethoxycarbonyl (Fmoc)-based solid-Phase Technique. The principle of this affinity-type purification procedure may also be applied to the tert-butoxycarbonyl (Boc)-based solid-Phase Technique.

Raffaele Liuzzi - One of the best experts on this subject based on the ideXlab platform.

  • Multislice CT with single-Phase Technique in patients with suspected pancreatic cancer.
    La Radiologia medica, 2006
    Co-Authors: Massimo Imbriaco, D. Smeraldo, Raffaele Liuzzi, F. Carrillo, G. Cacace, D. Vecchione, A. Diglio, Marco Salvatore
    Abstract:

    Purpose. The purpose of this study was to evaluate the role of multislice computed tomography (MSCT) with a single–Phase Technique in patients with suspected pancreatic cancer (PC).

  • Multislice CT with single–Phase Technique in patients with suspected pancreatic cancer
    La radiologia medica, 2006
    Co-Authors: Massimo Imbriaco, D. Smeraldo, Raffaele Liuzzi, F. Carrillo, G. Cacace, D. Vecchione, A. Diglio, Marco Salvatore
    Abstract:

    Purpose. The purpose of this study was to evaluate the role of multislice computed tomography (MSCT) with a single–Phase Technique in patients with suspected pancreatic cancer (PC). Materials and methods. Seventy–eight patients underwent MSCT with the following technical parameters: collimation: 4x1 mm; pitch 1; 120 kVp; 260 mAs. The pre–contrast scan was followed by a single acquisition Phase in the caudocranial direction from the inferior hepatic margin to the diaphragm with a 60–s delay after IV administration of 150 ml of iodinated contrast material at a rate of 3 ml/s. Two radiologists assessed the single images independently. Receiver operating characteristics (ROC) curves were obtained for each of the two observers. Results. The final diagnosis was pancreatic cancer in 46 cases and chronic pancreatitis in 32 cases. Areas under the curve (A_z) for diagnosis and evaluation of disease resectability were 0.97 and 0.93 for the first observer ( p =ns), and 0.97 and 0.90 for the second observer ( p =ns). The mean difference in tissue attenuation values between the cancer and normal pancreas was 72±3 Hounsfield units (HU). No statistically significant differences were observed in the degree of opacification between the peripancreatic arteries and veins. Conclusions. MSCT with a single–Phase Technique is an accurate and reproducible method for diagnosis and evaluation of disease resectability in patients with suspected PC, ensuring optimal tumour–to–pancreas contrast and maximal opacification of the main peripancreatic arterial and venous structures.

  • Value of the single-Phase Technique in MDCT assessment of pancreatic tumors.
    AJR. American journal of roentgenology, 2005
    Co-Authors: Massimo Imbriaco, Raffaele Liuzzi, Alec J. Megibow, Alfonso Ragozzino, P.p. Mainenti, Sara Bortone, Luigi Camera, Marco Salvatore
    Abstract:

    OBJECTIVE. The purpose of our study was to determine the diagnostic value of single-Phase MDCT in patients with suspected pancreatic carcinoma.SUBJECTS AND METHODS. Seventy-one patients (41 men, 30 women; mean age, 63 years; range, 29-80 years) with suspected pancreatic tumor underwent MDCT. Scanning was performed on an MDCT scanner with 0.5-sec gantry rotation and acquisition of 4 slices per rotation. Unenhanced scanning was followed by one set of scanning in the caudocranial direction from the inferior hepatic margin to the diaphragm with a scanning delay of 60 sec after the IV injection of 150 mL of contrast material delivered at 3 mL/sec. Two reviewers independently scored images in a blinded fashion for the presence of tumor and assessment of resectability. Receiver operating characteristic analysis was performed.RESULTS. A final histopathologic diagnosis derived from surgical findings was obtained in 42 patients; in the remaining 29 patients, percutaneous fine-needle aspiration biopsy coupled with a...

Susumu Funakoshi - One of the best experts on this subject based on the ideXlab platform.

  • Affinity purification method using a reversible biotinylating reagent for peptides synthesized by the solid-Phase Technique
    Journal of Chromatography A, 1993
    Co-Authors: Susumu Funakoshi, Hiroyuki Fukuda, Nobutaka Fujii
    Abstract:

    Abstract The specific interaction between biotin and avidin was explored as the basis of an affinity purification procedure for peptides synthesized by the solid-Phase Technique. In this affinity purification procedure, a reversible biotinylating reagent, 2-[(N-biotinyl)-aminoethylsulphonyl]ethyl p -nitrophenyl carbonate (BASEC), was synthesized. Using this reagent, the procedure involves the following sequence of four reactions: (i) attachment of the biotin to the N-terminus of the peptide-resin through a base-labile sulphonylethoxycarbonyl linkage at the final step of solid-Phase peptide synthesis; (ii) acid treatment to remove side-chain protecting groups and cleave the biotin-modified peptide from the resin; (iii) affinity purification of the biotinyl-peptide on an avidin-agarose colunm; and (iv) base (5% ammonia solution) treatment to remove the biotin moiety from the peptide. To facilitate this purification procedure, unreacted amino groups were acetylated in each step during solid-Phase synthesis. The usefulness of this method was demonstrated by the purification of several peptides synthesized by the 9-fluorenylmethoxycarbonyl (Fmoc)-based solid-Phase Technique.

  • Chemoselective one-step purification method for peptides synthesized by the solid-Phase Technique.
    Proceedings of the National Academy of Sciences of the United States of America, 1991
    Co-Authors: Susumu Funakoshi, Hiroyuki Fukuda, Nobutaka Fujii
    Abstract:

    Abstract The specific reaction between SH and iodoacetamide groups has been explored as the basis of an affinity-type purification procedure for peptides synthesized by the solid-Phase Technique. For this affinity-type purification procedure, we synthesized an SH precursor reagent bearing an acid-labile S-protecting group, pMB-SCH2CONHCH2CH2-SO2CH2CH2OCO2pNP (compound I), in which pMB is p-methoxybenzyl and pNP is p-nitrophenyl. Using this reagent, the procedure involves the following sequence of four reactions: (i) attachment of the SH function of compound I to the alpha-amino group of a peptide-resin through a base-labile sulfonylethoxycarbonyl linkage in the final step of solid-Phase peptide synthesis, (ii) acid treatment to remove the S-pMB and side-chain-protecting groups employed and cleave the modified peptide from the resin, (iii) immobilization of the derived SH-peptide on an iodoacetamide-resin column, and (iv) base (5% NH4OH) treatment to release the desired peptide from the resin in nearly pure form. To facilitate this purification procedure, unreacted amino groups were acetylated in each step during solid-Phase synthesis. The usefulness of this method was demonstrated by the purification of several peptides (18 to approximately 44 amino acids in length) synthesized by the 9-fluorenylmethoxycarbonyl (Fmoc)-based solid-Phase Technique. The principle of this affinity-type purification procedure may also be applied to the tert-butoxycarbonyl (Boc)-based solid-Phase Technique.