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Benny Lemaire - One of the best experts on this subject based on the ideXlab platform.
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characterization of the papilionoid burkholderia interaction in the fynbos biome the diversity and distribution of beta rhizobia nodulating podalyria calyptrata fabaceae podalyrieae
Systematic and Applied Microbiology, 2016Co-Authors: Benny Lemaire, Samson B. M. Chimphango, C.h. Stirton, Olivier Honnay, Erik Smets, Jannick Van Cauwenberghe, Brecht Verstraete, Janet I. SprentAbstract:The South African Fynbos soils are renowned for nitrogen-fixing Burkholderia associated with diverse papilionoid legumes of the tribes Crotalarieae, Hypocalypteae, Indigofereae, Phaseoleae and Podalyrieae. However, despite numerous rhizobial studies in the region, the symbiotic diversity of Burkholderia has not been investigated in relation to a specific host legume and its geographical provenance. This study analyzed the diversity of nodulating strains of Burkholderia from the legume species Podalyria calyptrata. Diverse lineages were detected that proved to be closely related to Burkholderia taxa, originating from hosts in other legume tribes. By analyzing the genetic variation of chromosomal (recA) and nodulation (nodA) sequence data in relation to the sampling sites we assessed the geographical distribution patterns of the P. calyptrata symbionts. Although we found a degree of genetically differentiated rhizobial populations, a correlation between genetic (recA and nodA) and geographic distances among populations was not observed, suggesting high rates of dispersal and rhizobial colonization within Fynbos soils.
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Symbiotic diversity, specificity and distribution of rhizobia in native legumes of the Core Cape Subregion (South Africa).
FEMS microbiology ecology, 2014Co-Authors: Benny Lemaire, Oscar Dlodlo, Samson B. M. Chimphango, C.h. Stirton, Brian Schrire, James S. Boatwright, Olivier Honnay, Erik Smets, Janet I. Sprent, Euan K JamesAbstract:Rhizobial diversity and host preferences were assessed in 65 native Fynbos legumes of the papilionoid legume tribes Astragaleae, Crotalarieae, Genisteae, Indigofereae, Millettieae, Phaseoleae, Podalyrieae, Psoraleeae and Sesbanieae. Sequence analyses of chromosomal 16S rRNA, recA, atpD and symbiosis-related nodA , nifH genes in parallel with immunogold labelling assays identified the symbionts as alpha- ( Azorhizobium , Bradyrhizobium , Ensifer, Mesorhizobium and Rhizobium ) and beta-rhizobial ( Burkholderia ) lineages with the majority placed in the genera Mesorhizobium and Burkholderia showing a wide range of host interactions. Despite a degree of symbiotic promiscuity in the tribes Crotalarieae and Indigofereae nodulating with both alpha- and beta-rhizobia, Mesorhizobium symbionts appeared to exhibit a general host preference for the tribe Psoraleeae, whereas Burkholderia prevailed in the Podalyrieae. Although host genotype was the main factor determining rhizobial diversity, ecological factors such as soil acidity and site elevation were positively correlated with genetic variation within Mesorhizobium and Burkholderia , respectively, indicating an interplay of host and environmental factors on the distribution of Fynbos rhizobia.
Janet I. Sprent - One of the best experts on this subject based on the ideXlab platform.
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characterization of the papilionoid burkholderia interaction in the fynbos biome the diversity and distribution of beta rhizobia nodulating podalyria calyptrata fabaceae podalyrieae
Systematic and Applied Microbiology, 2016Co-Authors: Benny Lemaire, Samson B. M. Chimphango, C.h. Stirton, Olivier Honnay, Erik Smets, Jannick Van Cauwenberghe, Brecht Verstraete, Janet I. SprentAbstract:The South African Fynbos soils are renowned for nitrogen-fixing Burkholderia associated with diverse papilionoid legumes of the tribes Crotalarieae, Hypocalypteae, Indigofereae, Phaseoleae and Podalyrieae. However, despite numerous rhizobial studies in the region, the symbiotic diversity of Burkholderia has not been investigated in relation to a specific host legume and its geographical provenance. This study analyzed the diversity of nodulating strains of Burkholderia from the legume species Podalyria calyptrata. Diverse lineages were detected that proved to be closely related to Burkholderia taxa, originating from hosts in other legume tribes. By analyzing the genetic variation of chromosomal (recA) and nodulation (nodA) sequence data in relation to the sampling sites we assessed the geographical distribution patterns of the P. calyptrata symbionts. Although we found a degree of genetically differentiated rhizobial populations, a correlation between genetic (recA and nodA) and geographic distances among populations was not observed, suggesting high rates of dispersal and rhizobial colonization within Fynbos soils.
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Nodulation of Cyclopia spp. (Leguminosae, Papilionoideae) by Burkholderia tuberum
2015Co-Authors: Geoffrey N. Elliott, Janet I. Sprent, Wen-ming Chen, Cyril Bontemps, Jui-hsing Chou, J Peter, W. Young, Euan K JamesAbstract:legume nodules, but so far they have only been shown to form symbioses with species of Mimosa, sub-family Mimosoideae. This work investigates whether Burkholderia tuberum strains STM678 (isolated from Aspalathus carnosa) and DUS833 (from Aspalathus callosa) can nodulate species of the South African endemic papilionoid genera Cyclopia (tribe Podalyrieae) and Aspalathus (Crotalarieae) as well as the promiscuous legume Macroptilium atropurpureum (Phaseoleae). †Methods Bacterial strains and the phylogeny of their symbiosis-related (nod) genes were examined via 16S rRNA gene sequencing. Seedlings were grown in liquid culture and inoculated with one of the two strains of B. tuberum or with Sinorhizobium strain NGR 234 (from Lablab purpureus), Mesorhizobium strain DUS835 (from Aspalathus linearis) or Methylobacterium nodulans (from Crotalaria podocarpa). Some nodules, inoculated with green fluor-escence protein (GFP)-tagged strains, were examined by light and electron microscopy coupled with immunogold lab-elling with a Burkholderia-specific antibody. The presence of active nitrogenase was checked by immunolabelling of nitrogenase and by the acetylene reduction assay.B. tuberum STM678 was also tested on a wide range of legumes from all three sub-families. †Key Results Nodules were not formed on any of the Aspalathus spp. Only B. tuberum nodulated Cyclopia falcata, C. galioides, C. genistoides, C. intermedia and C. pubescens. It also effectively nodulated M. atropurpureum bu
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Symbiotic diversity, specificity and distribution of rhizobia in native legumes of the Core Cape Subregion (South Africa).
FEMS microbiology ecology, 2014Co-Authors: Benny Lemaire, Oscar Dlodlo, Samson B. M. Chimphango, C.h. Stirton, Brian Schrire, James S. Boatwright, Olivier Honnay, Erik Smets, Janet I. Sprent, Euan K JamesAbstract:Rhizobial diversity and host preferences were assessed in 65 native Fynbos legumes of the papilionoid legume tribes Astragaleae, Crotalarieae, Genisteae, Indigofereae, Millettieae, Phaseoleae, Podalyrieae, Psoraleeae and Sesbanieae. Sequence analyses of chromosomal 16S rRNA, recA, atpD and symbiosis-related nodA , nifH genes in parallel with immunogold labelling assays identified the symbionts as alpha- ( Azorhizobium , Bradyrhizobium , Ensifer, Mesorhizobium and Rhizobium ) and beta-rhizobial ( Burkholderia ) lineages with the majority placed in the genera Mesorhizobium and Burkholderia showing a wide range of host interactions. Despite a degree of symbiotic promiscuity in the tribes Crotalarieae and Indigofereae nodulating with both alpha- and beta-rhizobia, Mesorhizobium symbionts appeared to exhibit a general host preference for the tribe Psoraleeae, whereas Burkholderia prevailed in the Podalyrieae. Although host genotype was the main factor determining rhizobial diversity, ecological factors such as soil acidity and site elevation were positively correlated with genetic variation within Mesorhizobium and Burkholderia , respectively, indicating an interplay of host and environmental factors on the distribution of Fynbos rhizobia.
Erik Smets - One of the best experts on this subject based on the ideXlab platform.
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characterization of the papilionoid burkholderia interaction in the fynbos biome the diversity and distribution of beta rhizobia nodulating podalyria calyptrata fabaceae podalyrieae
Systematic and Applied Microbiology, 2016Co-Authors: Benny Lemaire, Samson B. M. Chimphango, C.h. Stirton, Olivier Honnay, Erik Smets, Jannick Van Cauwenberghe, Brecht Verstraete, Janet I. SprentAbstract:The South African Fynbos soils are renowned for nitrogen-fixing Burkholderia associated with diverse papilionoid legumes of the tribes Crotalarieae, Hypocalypteae, Indigofereae, Phaseoleae and Podalyrieae. However, despite numerous rhizobial studies in the region, the symbiotic diversity of Burkholderia has not been investigated in relation to a specific host legume and its geographical provenance. This study analyzed the diversity of nodulating strains of Burkholderia from the legume species Podalyria calyptrata. Diverse lineages were detected that proved to be closely related to Burkholderia taxa, originating from hosts in other legume tribes. By analyzing the genetic variation of chromosomal (recA) and nodulation (nodA) sequence data in relation to the sampling sites we assessed the geographical distribution patterns of the P. calyptrata symbionts. Although we found a degree of genetically differentiated rhizobial populations, a correlation between genetic (recA and nodA) and geographic distances among populations was not observed, suggesting high rates of dispersal and rhizobial colonization within Fynbos soils.
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Symbiotic diversity, specificity and distribution of rhizobia in native legumes of the Core Cape Subregion (South Africa).
FEMS microbiology ecology, 2014Co-Authors: Benny Lemaire, Oscar Dlodlo, Samson B. M. Chimphango, C.h. Stirton, Brian Schrire, James S. Boatwright, Olivier Honnay, Erik Smets, Janet I. Sprent, Euan K JamesAbstract:Rhizobial diversity and host preferences were assessed in 65 native Fynbos legumes of the papilionoid legume tribes Astragaleae, Crotalarieae, Genisteae, Indigofereae, Millettieae, Phaseoleae, Podalyrieae, Psoraleeae and Sesbanieae. Sequence analyses of chromosomal 16S rRNA, recA, atpD and symbiosis-related nodA , nifH genes in parallel with immunogold labelling assays identified the symbionts as alpha- ( Azorhizobium , Bradyrhizobium , Ensifer, Mesorhizobium and Rhizobium ) and beta-rhizobial ( Burkholderia ) lineages with the majority placed in the genera Mesorhizobium and Burkholderia showing a wide range of host interactions. Despite a degree of symbiotic promiscuity in the tribes Crotalarieae and Indigofereae nodulating with both alpha- and beta-rhizobia, Mesorhizobium symbionts appeared to exhibit a general host preference for the tribe Psoraleeae, whereas Burkholderia prevailed in the Podalyrieae. Although host genotype was the main factor determining rhizobial diversity, ecological factors such as soil acidity and site elevation were positively correlated with genetic variation within Mesorhizobium and Burkholderia , respectively, indicating an interplay of host and environmental factors on the distribution of Fynbos rhizobia.
Samson B. M. Chimphango - One of the best experts on this subject based on the ideXlab platform.
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characterization of the papilionoid burkholderia interaction in the fynbos biome the diversity and distribution of beta rhizobia nodulating podalyria calyptrata fabaceae podalyrieae
Systematic and Applied Microbiology, 2016Co-Authors: Benny Lemaire, Samson B. M. Chimphango, C.h. Stirton, Olivier Honnay, Erik Smets, Jannick Van Cauwenberghe, Brecht Verstraete, Janet I. SprentAbstract:The South African Fynbos soils are renowned for nitrogen-fixing Burkholderia associated with diverse papilionoid legumes of the tribes Crotalarieae, Hypocalypteae, Indigofereae, Phaseoleae and Podalyrieae. However, despite numerous rhizobial studies in the region, the symbiotic diversity of Burkholderia has not been investigated in relation to a specific host legume and its geographical provenance. This study analyzed the diversity of nodulating strains of Burkholderia from the legume species Podalyria calyptrata. Diverse lineages were detected that proved to be closely related to Burkholderia taxa, originating from hosts in other legume tribes. By analyzing the genetic variation of chromosomal (recA) and nodulation (nodA) sequence data in relation to the sampling sites we assessed the geographical distribution patterns of the P. calyptrata symbionts. Although we found a degree of genetically differentiated rhizobial populations, a correlation between genetic (recA and nodA) and geographic distances among populations was not observed, suggesting high rates of dispersal and rhizobial colonization within Fynbos soils.
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Symbiotic diversity, specificity and distribution of rhizobia in native legumes of the Core Cape Subregion (South Africa).
FEMS microbiology ecology, 2014Co-Authors: Benny Lemaire, Oscar Dlodlo, Samson B. M. Chimphango, C.h. Stirton, Brian Schrire, James S. Boatwright, Olivier Honnay, Erik Smets, Janet I. Sprent, Euan K JamesAbstract:Rhizobial diversity and host preferences were assessed in 65 native Fynbos legumes of the papilionoid legume tribes Astragaleae, Crotalarieae, Genisteae, Indigofereae, Millettieae, Phaseoleae, Podalyrieae, Psoraleeae and Sesbanieae. Sequence analyses of chromosomal 16S rRNA, recA, atpD and symbiosis-related nodA , nifH genes in parallel with immunogold labelling assays identified the symbionts as alpha- ( Azorhizobium , Bradyrhizobium , Ensifer, Mesorhizobium and Rhizobium ) and beta-rhizobial ( Burkholderia ) lineages with the majority placed in the genera Mesorhizobium and Burkholderia showing a wide range of host interactions. Despite a degree of symbiotic promiscuity in the tribes Crotalarieae and Indigofereae nodulating with both alpha- and beta-rhizobia, Mesorhizobium symbionts appeared to exhibit a general host preference for the tribe Psoraleeae, whereas Burkholderia prevailed in the Podalyrieae. Although host genotype was the main factor determining rhizobial diversity, ecological factors such as soil acidity and site elevation were positively correlated with genetic variation within Mesorhizobium and Burkholderia , respectively, indicating an interplay of host and environmental factors on the distribution of Fynbos rhizobia.
C.h. Stirton - One of the best experts on this subject based on the ideXlab platform.
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characterization of the papilionoid burkholderia interaction in the fynbos biome the diversity and distribution of beta rhizobia nodulating podalyria calyptrata fabaceae podalyrieae
Systematic and Applied Microbiology, 2016Co-Authors: Benny Lemaire, Samson B. M. Chimphango, C.h. Stirton, Olivier Honnay, Erik Smets, Jannick Van Cauwenberghe, Brecht Verstraete, Janet I. SprentAbstract:The South African Fynbos soils are renowned for nitrogen-fixing Burkholderia associated with diverse papilionoid legumes of the tribes Crotalarieae, Hypocalypteae, Indigofereae, Phaseoleae and Podalyrieae. However, despite numerous rhizobial studies in the region, the symbiotic diversity of Burkholderia has not been investigated in relation to a specific host legume and its geographical provenance. This study analyzed the diversity of nodulating strains of Burkholderia from the legume species Podalyria calyptrata. Diverse lineages were detected that proved to be closely related to Burkholderia taxa, originating from hosts in other legume tribes. By analyzing the genetic variation of chromosomal (recA) and nodulation (nodA) sequence data in relation to the sampling sites we assessed the geographical distribution patterns of the P. calyptrata symbionts. Although we found a degree of genetically differentiated rhizobial populations, a correlation between genetic (recA and nodA) and geographic distances among populations was not observed, suggesting high rates of dispersal and rhizobial colonization within Fynbos soils.
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Symbiotic diversity, specificity and distribution of rhizobia in native legumes of the Core Cape Subregion (South Africa).
FEMS microbiology ecology, 2014Co-Authors: Benny Lemaire, Oscar Dlodlo, Samson B. M. Chimphango, C.h. Stirton, Brian Schrire, James S. Boatwright, Olivier Honnay, Erik Smets, Janet I. Sprent, Euan K JamesAbstract:Rhizobial diversity and host preferences were assessed in 65 native Fynbos legumes of the papilionoid legume tribes Astragaleae, Crotalarieae, Genisteae, Indigofereae, Millettieae, Phaseoleae, Podalyrieae, Psoraleeae and Sesbanieae. Sequence analyses of chromosomal 16S rRNA, recA, atpD and symbiosis-related nodA , nifH genes in parallel with immunogold labelling assays identified the symbionts as alpha- ( Azorhizobium , Bradyrhizobium , Ensifer, Mesorhizobium and Rhizobium ) and beta-rhizobial ( Burkholderia ) lineages with the majority placed in the genera Mesorhizobium and Burkholderia showing a wide range of host interactions. Despite a degree of symbiotic promiscuity in the tribes Crotalarieae and Indigofereae nodulating with both alpha- and beta-rhizobia, Mesorhizobium symbionts appeared to exhibit a general host preference for the tribe Psoraleeae, whereas Burkholderia prevailed in the Podalyrieae. Although host genotype was the main factor determining rhizobial diversity, ecological factors such as soil acidity and site elevation were positively correlated with genetic variation within Mesorhizobium and Burkholderia , respectively, indicating an interplay of host and environmental factors on the distribution of Fynbos rhizobia.