The Experts below are selected from a list of 1314 Experts worldwide ranked by ideXlab platform
Jicheng Zhan - One of the best experts on this subject based on the ideXlab platform.
-
melatonin and Phenolics Biosynthesis related genes in vitis vinifera cell suspension cultures are regulated by temperature and copper stress
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Lihua Wang, Weidong Huang, Jicheng ZhanAbstract:Temperature and copper stress are two common abiotic stresses in viticulture. To better understand Biosynthesis of melatonin and Phenolics in response to these two abiotic stresses, cell suspension cultures of Vitis vinifera were treated with high temperature (HT, 38 °C), low temperature (LT, 16 °C) and 100 μM CuSO4 to investigate whether genes associated with the Biosynthesis of melatonin (TDC, SNAT and ASMT) and Phenolics (PAL, C4H, CHS, CHI and STS) were involved in response to these two abiotic stresses, as well as the effects of these two stresses on total Phenolics, total flavonoids contents and oxidative stress. The expression patterns indicated that TDC, SNAT, ASMT, PAL and C4H were significantly upregulated in response to HT, while CHS and CHI first increased and then decreased, and STS was significantly downregulated. SNAT was significantly upregulated in response to LT, while TDC, ASMT, C4H and CHS first increased and then decreased, and PAL, CHI and STS were significantly downregulated. Copper treatment significantly induced the expression of TDC, SNAT and ASMT, and ASMT was the most significantly induced. C4H, CHI and STS were significantly upregulated in response to copper treatment, while PAL first increased and then decreased. Both HT and LT treatment significantly enhanced the contents of total Phenolics and total flavonoids. The malondialdehyde (MDA) content of the cells treated with HT significantly increased. The treatment with CuSO4 significantly enhanced the contents of MDA and H2O2 in the cells. The results suggest that melatonin and Phenolics Biosynthesis-related genes were involved in HT, LT and copper stress response. The same gene showed different response patterns to the different stresses. HT and copper stress may cause oxidative stress. Whether the Biosynthesis of melatonin and Phenolics in grapes was also involved in response to temperature and copper stress will provide inspiration for the practice of stress-resistant viticulture
Ai-xia Cheng - One of the best experts on this subject based on the ideXlab platform.
-
Cloning and Functional Characterization of Two 4-Coumarate: CoA Ligase Genes from Selaginella moellendorffii.
Molecules, 2018Co-Authors: Ping-ping Wang, Yi-feng Wu, Ai-xia ChengAbstract:Selaginella is an extant lycopodiophyte genus, which is representative of an ancient lineage of tracheophytes. The important evolutionary status makes it a valuable resource for the study of metabolic evolution in vascular plants. 4-coumarate: CoA ligase (4CL) is the pivotal enzyme that controls the flow of carbon through the phenylpropanoid metabolic pathway into the specific lignin, flavonoid, and wall-bound Phenolics Biosynthesis pathways. Although 4CLs have been extensively characterized in other vascular plants, little is known of their functions in Selaginella. Here, we isolated two 4CL genes (Sm4CL1 and Sm4CL2) from Selaginella moellendorffii. Based on the enzymatic activities of the recombinant proteins, both of these genes encoded bona fide 4CLs. The 4CL isoforms in S. moellendorffii have different activities: Sm4CL2 was more active than Sm4CL1. The enzymatic properties and gene expression patterns indicated that the 4CL genes have been conserved in the evolution of vascular plants.
Lihua Wang - One of the best experts on this subject based on the ideXlab platform.
-
melatonin and Phenolics Biosynthesis related genes in vitis vinifera cell suspension cultures are regulated by temperature and copper stress
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Lihua Wang, Weidong Huang, Jicheng ZhanAbstract:Temperature and copper stress are two common abiotic stresses in viticulture. To better understand Biosynthesis of melatonin and Phenolics in response to these two abiotic stresses, cell suspension cultures of Vitis vinifera were treated with high temperature (HT, 38 °C), low temperature (LT, 16 °C) and 100 μM CuSO4 to investigate whether genes associated with the Biosynthesis of melatonin (TDC, SNAT and ASMT) and Phenolics (PAL, C4H, CHS, CHI and STS) were involved in response to these two abiotic stresses, as well as the effects of these two stresses on total Phenolics, total flavonoids contents and oxidative stress. The expression patterns indicated that TDC, SNAT, ASMT, PAL and C4H were significantly upregulated in response to HT, while CHS and CHI first increased and then decreased, and STS was significantly downregulated. SNAT was significantly upregulated in response to LT, while TDC, ASMT, C4H and CHS first increased and then decreased, and PAL, CHI and STS were significantly downregulated. Copper treatment significantly induced the expression of TDC, SNAT and ASMT, and ASMT was the most significantly induced. C4H, CHI and STS were significantly upregulated in response to copper treatment, while PAL first increased and then decreased. Both HT and LT treatment significantly enhanced the contents of total Phenolics and total flavonoids. The malondialdehyde (MDA) content of the cells treated with HT significantly increased. The treatment with CuSO4 significantly enhanced the contents of MDA and H2O2 in the cells. The results suggest that melatonin and Phenolics Biosynthesis-related genes were involved in HT, LT and copper stress response. The same gene showed different response patterns to the different stresses. HT and copper stress may cause oxidative stress. Whether the Biosynthesis of melatonin and Phenolics in grapes was also involved in response to temperature and copper stress will provide inspiration for the practice of stress-resistant viticulture
Ping-ping Wang - One of the best experts on this subject based on the ideXlab platform.
-
Cloning and Functional Characterization of Two 4-Coumarate: CoA Ligase Genes from Selaginella moellendorffii.
Molecules, 2018Co-Authors: Ping-ping Wang, Yi-feng Wu, Ai-xia ChengAbstract:Selaginella is an extant lycopodiophyte genus, which is representative of an ancient lineage of tracheophytes. The important evolutionary status makes it a valuable resource for the study of metabolic evolution in vascular plants. 4-coumarate: CoA ligase (4CL) is the pivotal enzyme that controls the flow of carbon through the phenylpropanoid metabolic pathway into the specific lignin, flavonoid, and wall-bound Phenolics Biosynthesis pathways. Although 4CLs have been extensively characterized in other vascular plants, little is known of their functions in Selaginella. Here, we isolated two 4CL genes (Sm4CL1 and Sm4CL2) from Selaginella moellendorffii. Based on the enzymatic activities of the recombinant proteins, both of these genes encoded bona fide 4CLs. The 4CL isoforms in S. moellendorffii have different activities: Sm4CL2 was more active than Sm4CL1. The enzymatic properties and gene expression patterns indicated that the 4CL genes have been conserved in the evolution of vascular plants.
Weidong Huang - One of the best experts on this subject based on the ideXlab platform.
-
melatonin and Phenolics Biosynthesis related genes in vitis vinifera cell suspension cultures are regulated by temperature and copper stress
Plant Cell Tissue and Organ Culture, 2019Co-Authors: Lihua Wang, Weidong Huang, Jicheng ZhanAbstract:Temperature and copper stress are two common abiotic stresses in viticulture. To better understand Biosynthesis of melatonin and Phenolics in response to these two abiotic stresses, cell suspension cultures of Vitis vinifera were treated with high temperature (HT, 38 °C), low temperature (LT, 16 °C) and 100 μM CuSO4 to investigate whether genes associated with the Biosynthesis of melatonin (TDC, SNAT and ASMT) and Phenolics (PAL, C4H, CHS, CHI and STS) were involved in response to these two abiotic stresses, as well as the effects of these two stresses on total Phenolics, total flavonoids contents and oxidative stress. The expression patterns indicated that TDC, SNAT, ASMT, PAL and C4H were significantly upregulated in response to HT, while CHS and CHI first increased and then decreased, and STS was significantly downregulated. SNAT was significantly upregulated in response to LT, while TDC, ASMT, C4H and CHS first increased and then decreased, and PAL, CHI and STS were significantly downregulated. Copper treatment significantly induced the expression of TDC, SNAT and ASMT, and ASMT was the most significantly induced. C4H, CHI and STS were significantly upregulated in response to copper treatment, while PAL first increased and then decreased. Both HT and LT treatment significantly enhanced the contents of total Phenolics and total flavonoids. The malondialdehyde (MDA) content of the cells treated with HT significantly increased. The treatment with CuSO4 significantly enhanced the contents of MDA and H2O2 in the cells. The results suggest that melatonin and Phenolics Biosynthesis-related genes were involved in HT, LT and copper stress response. The same gene showed different response patterns to the different stresses. HT and copper stress may cause oxidative stress. Whether the Biosynthesis of melatonin and Phenolics in grapes was also involved in response to temperature and copper stress will provide inspiration for the practice of stress-resistant viticulture