The Experts below are selected from a list of 342 Experts worldwide ranked by ideXlab platform
Takemi Yoshida - One of the best experts on this subject based on the ideXlab platform.
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Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5
Elsevier, 2014Co-Authors: Takashi Ashino, Takemi Yoshida, Haruyo Ohkubo-morita, Masayuki Yamamoto, Satoshi NumazawaAbstract:Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)–deficient (Nrf2−⧸−) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased Cyp2b10 and Cyp2a5 mRNA as well as Nrf2 target genes, including NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1, in wild-type mouse livers 8 h after treatment. The Phorone-induced mRNA levels in Nrf2−⧸− mouse livers were lower than that in wild-type mouse livers. Nrf2−⧸− mice showed attenuated Cyp2b10 and Cyp2a5 induction by phenobarbital, a classical Cyp2b inducer. These findings suggest that the Nrf2 pathway is involved in Cyp2b10 and Cyp2a5 gene expression
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the expression of heme oxygenase 1 gene responded to oxidative stress produced by Phorone a glutathione depletor in the rat liver the relevance to activation of c jun n terminal kinase
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Takiko Oguro, Moyuru Hayashi, Shigeo Nakajo, Satoshi Numazawa, Takemi YoshidaAbstract:Phorone, a glutathione (GSH) depletor, induces the expression of mRNAs of heme oxygenase-1 (HO-1) and c-jun by mediating the activation of activated protein-1 (AP-1) in rat livers. We have shown that Phorone activates c-Jun N-terminal kinase (JNK), thus leading to c-Jun phosphorylation, and transactivation of AP-1 and HO-1 gene expression in the rat liver in response to oxidative stress. The in-gel kinase assay showed that Phorone activated JNK1 predominantly in the rat liver nuclear extract. The JNK activation by Phorone was slightly observed at 1 hr after administration and gradually increased with time. Ser73-phosphorylation of c-Jun catalyzed by JNK was significantly altered by changing hepatic GSH levels based on the results observed by the combined injection of buthionine sulfoximine (BSO) or GSH isopropyl ester (GIP) with Phorone. Namely, BSO, an inhibitor of GSH biosynthesis, enhanced Phorone-mediated c-Jun phosphorylation as well as AP-1 binding activity. However, GSH isopropyl ester prevented GSH depletion and abolished both c-Jun phosphorylation and the activation of AP-1 binding evoked by Phorone. GSH isopropyl ester also suppressed Phorone-produced HO-1 and c-jun gene expressions to 25 and 30% of the induced level. Perfluorodecanoic acid (PFDA) reduced GSH S-transferase activity, prevented Phorone-mediated GSH depletion and abolished either HO-1 or c-jun mRNA induction by Phorone. These results indicated that oxidative stress under GSH depletion produced by Phorone could activate preferentially JNK and lead to the transcriptional activation of AP-1 and consequently to HO-1 gene expression. This study suggests that JNK activation could be one of the major signaling pathways to transmit intracellular events to the nuclei during oxidative stress via GSH depletion by Phorone in rat livers.
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heme oxygenase 1 gene expression by a glutathione depletor Phorone mediated through ap 1 activation in rats
Biochemical and Biophysical Research Communications, 1996Co-Authors: Takiko Oguro, Moyuru Hayashi, Satoshi Numazawa, Kiyoshi Asakawa, Takemi YoshidaAbstract:Abstract This study shows that induction of heme oxygenase-1 (HO-1) gene expression by a glutathione (GSH) depletor, Phorone, is inhibited by cycloheximide pretreatment and involves changes in c-jun, not c-fos, mRNA. Buthionine sulfoximine (BSO) enhanced markedly both c-jun and HO-1 gene expression evoked by Phorone. Phorone dramatically increased AP-1 binding activity, which was blocked by unlabeled AP-1 oligonucleotide and abolished by anti-Jun antibodies, but not anti-Fos antibodies. In addition, pretreatment with dexamethasone, an inhibitor of AP-1 DNA binding, inhibited Phorone-mediated HO-1 mRNA induction. These findings suggest that HO-1 induction by Phorone is likely to involve in the activation of AP-1(Jun/Jun) binding, which could be associating with GSH depletion.
Satoshi Numazawa - One of the best experts on this subject based on the ideXlab platform.
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Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5
Elsevier, 2014Co-Authors: Takashi Ashino, Takemi Yoshida, Haruyo Ohkubo-morita, Masayuki Yamamoto, Satoshi NumazawaAbstract:Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)–deficient (Nrf2−⧸−) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased Cyp2b10 and Cyp2a5 mRNA as well as Nrf2 target genes, including NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1, in wild-type mouse livers 8 h after treatment. The Phorone-induced mRNA levels in Nrf2−⧸− mouse livers were lower than that in wild-type mouse livers. Nrf2−⧸− mice showed attenuated Cyp2b10 and Cyp2a5 induction by phenobarbital, a classical Cyp2b inducer. These findings suggest that the Nrf2 pathway is involved in Cyp2b10 and Cyp2a5 gene expression
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Keywords: Nuclear-factor erythroid 2-related factor 2
2014Co-Authors: Takashi Ashino A, Haruyo Ohkubo-morita A, Masayuki Yamamoto B, Takemi Yoshida A, Satoshi NumazawaAbstract:a b s t r a c t Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)–deficient (Nrf2⧸) mice to investigate the involve-ment of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased Cyp2b10 and Cyp2a5 mRNA as well as Nrf2 target genes, including NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1, in wild-type mouse livers 8 h after treatment. The Phorone-induced mRNA levels in Nrf2⧸ mouse livers were lower than that in wild-type mouse livers. Nrf2⧸ mice showed attenuated Cyp2b10 and Cyp2a5 induction by phenobarbital, a classical Cyp2b inducer. These findings suggest that the Nrf2 pathway is involved in Cyp2b10 and Cyp2a5 gene expression. & 2014 The Authors. Published by Elsevier B.V. All rights reserved
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the expression of heme oxygenase 1 gene responded to oxidative stress produced by Phorone a glutathione depletor in the rat liver the relevance to activation of c jun n terminal kinase
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Takiko Oguro, Moyuru Hayashi, Shigeo Nakajo, Satoshi Numazawa, Takemi YoshidaAbstract:Phorone, a glutathione (GSH) depletor, induces the expression of mRNAs of heme oxygenase-1 (HO-1) and c-jun by mediating the activation of activated protein-1 (AP-1) in rat livers. We have shown that Phorone activates c-Jun N-terminal kinase (JNK), thus leading to c-Jun phosphorylation, and transactivation of AP-1 and HO-1 gene expression in the rat liver in response to oxidative stress. The in-gel kinase assay showed that Phorone activated JNK1 predominantly in the rat liver nuclear extract. The JNK activation by Phorone was slightly observed at 1 hr after administration and gradually increased with time. Ser73-phosphorylation of c-Jun catalyzed by JNK was significantly altered by changing hepatic GSH levels based on the results observed by the combined injection of buthionine sulfoximine (BSO) or GSH isopropyl ester (GIP) with Phorone. Namely, BSO, an inhibitor of GSH biosynthesis, enhanced Phorone-mediated c-Jun phosphorylation as well as AP-1 binding activity. However, GSH isopropyl ester prevented GSH depletion and abolished both c-Jun phosphorylation and the activation of AP-1 binding evoked by Phorone. GSH isopropyl ester also suppressed Phorone-produced HO-1 and c-jun gene expressions to 25 and 30% of the induced level. Perfluorodecanoic acid (PFDA) reduced GSH S-transferase activity, prevented Phorone-mediated GSH depletion and abolished either HO-1 or c-jun mRNA induction by Phorone. These results indicated that oxidative stress under GSH depletion produced by Phorone could activate preferentially JNK and lead to the transcriptional activation of AP-1 and consequently to HO-1 gene expression. This study suggests that JNK activation could be one of the major signaling pathways to transmit intracellular events to the nuclei during oxidative stress via GSH depletion by Phorone in rat livers.
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heme oxygenase 1 gene expression by a glutathione depletor Phorone mediated through ap 1 activation in rats
Biochemical and Biophysical Research Communications, 1996Co-Authors: Takiko Oguro, Moyuru Hayashi, Satoshi Numazawa, Kiyoshi Asakawa, Takemi YoshidaAbstract:Abstract This study shows that induction of heme oxygenase-1 (HO-1) gene expression by a glutathione (GSH) depletor, Phorone, is inhibited by cycloheximide pretreatment and involves changes in c-jun, not c-fos, mRNA. Buthionine sulfoximine (BSO) enhanced markedly both c-jun and HO-1 gene expression evoked by Phorone. Phorone dramatically increased AP-1 binding activity, which was blocked by unlabeled AP-1 oligonucleotide and abolished by anti-Jun antibodies, but not anti-Fos antibodies. In addition, pretreatment with dexamethasone, an inhibitor of AP-1 DNA binding, inhibited Phorone-mediated HO-1 mRNA induction. These findings suggest that HO-1 induction by Phorone is likely to involve in the activation of AP-1(Jun/Jun) binding, which could be associating with GSH depletion.
Takiko Oguro - One of the best experts on this subject based on the ideXlab platform.
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the expression of heme oxygenase 1 gene responded to oxidative stress produced by Phorone a glutathione depletor in the rat liver the relevance to activation of c jun n terminal kinase
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Takiko Oguro, Moyuru Hayashi, Shigeo Nakajo, Satoshi Numazawa, Takemi YoshidaAbstract:Phorone, a glutathione (GSH) depletor, induces the expression of mRNAs of heme oxygenase-1 (HO-1) and c-jun by mediating the activation of activated protein-1 (AP-1) in rat livers. We have shown that Phorone activates c-Jun N-terminal kinase (JNK), thus leading to c-Jun phosphorylation, and transactivation of AP-1 and HO-1 gene expression in the rat liver in response to oxidative stress. The in-gel kinase assay showed that Phorone activated JNK1 predominantly in the rat liver nuclear extract. The JNK activation by Phorone was slightly observed at 1 hr after administration and gradually increased with time. Ser73-phosphorylation of c-Jun catalyzed by JNK was significantly altered by changing hepatic GSH levels based on the results observed by the combined injection of buthionine sulfoximine (BSO) or GSH isopropyl ester (GIP) with Phorone. Namely, BSO, an inhibitor of GSH biosynthesis, enhanced Phorone-mediated c-Jun phosphorylation as well as AP-1 binding activity. However, GSH isopropyl ester prevented GSH depletion and abolished both c-Jun phosphorylation and the activation of AP-1 binding evoked by Phorone. GSH isopropyl ester also suppressed Phorone-produced HO-1 and c-jun gene expressions to 25 and 30% of the induced level. Perfluorodecanoic acid (PFDA) reduced GSH S-transferase activity, prevented Phorone-mediated GSH depletion and abolished either HO-1 or c-jun mRNA induction by Phorone. These results indicated that oxidative stress under GSH depletion produced by Phorone could activate preferentially JNK and lead to the transcriptional activation of AP-1 and consequently to HO-1 gene expression. This study suggests that JNK activation could be one of the major signaling pathways to transmit intracellular events to the nuclei during oxidative stress via GSH depletion by Phorone in rat livers.
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heme oxygenase 1 gene expression by a glutathione depletor Phorone mediated through ap 1 activation in rats
Biochemical and Biophysical Research Communications, 1996Co-Authors: Takiko Oguro, Moyuru Hayashi, Satoshi Numazawa, Kiyoshi Asakawa, Takemi YoshidaAbstract:Abstract This study shows that induction of heme oxygenase-1 (HO-1) gene expression by a glutathione (GSH) depletor, Phorone, is inhibited by cycloheximide pretreatment and involves changes in c-jun, not c-fos, mRNA. Buthionine sulfoximine (BSO) enhanced markedly both c-jun and HO-1 gene expression evoked by Phorone. Phorone dramatically increased AP-1 binding activity, which was blocked by unlabeled AP-1 oligonucleotide and abolished by anti-Jun antibodies, but not anti-Fos antibodies. In addition, pretreatment with dexamethasone, an inhibitor of AP-1 DNA binding, inhibited Phorone-mediated HO-1 mRNA induction. These findings suggest that HO-1 induction by Phorone is likely to involve in the activation of AP-1(Jun/Jun) binding, which could be associating with GSH depletion.
Erik C Andersen - One of the best experts on this subject based on the ideXlab platform.
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the nematode caenorhabditis elegans and the terrestrial isopod porcellio scaber likely interact opportunistically
PLOS ONE, 2020Co-Authors: Heather Archer, Selina Deiparine, Erik C AndersenAbstract:Phoresy is a behavior in which an organism, the phoront, travels from one location to another by 'hitching a ride' on the body of a host as it disperses. Some phoronts are generalists, taking advantage of any available host. Others are specialists and travel only when specific hosts are located using chemical cues to identify and move (chemotax) toward the preferred host. Free-living nematodes, like Caenorhabditis elegans, are often found in natural environments that contain terrestrial isopods and other invertebrates. Additionally, the C. elegans wild strain PB306 was isolated associated with the isopod Porcellio scaber. However, it is currently unclear if C. elegans is a phoront of terrestrial isopods, and if so, whether it is a specialist, generalist, or developmental stage-specific combination of both strategies. Because the relevant chemical stimuli might be secreted compounds or volatile odorants, we used different types of chemotaxis assays across diverse extractions of compounds or odorants to test whether C. elegans is attracted to P. scaber. We show that two different strains-the wild isolate PB306 and the laboratory-adapted strain N2 -are not attracted to P. scaber during either the dauer or adult life stages. Our results indicate that C. elegans was not attracted to chemical compounds or volatile odorants from P. scaber, providing valuable empirical evidence to suggest that any associations between these two species are likely opportunistic rather than specific phoresy.
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caenorhabditis elegans nematodes are not attracted to the terrestrial isopod porcellio scaber
bioRxiv, 2020Co-Authors: Heather Archer, Selina Deiparine, Erik C AndersenAbstract:Phoresy is a behavior in which an organism, the phoront, travels from one location to another by 9hitching a ride9 on the body of a host as it disperses. Some phoronts are generalists, taking advantage of any available host. Others are specialists and travel only when specific hosts are located using chemical cues to identify and move (chemotax) toward the preferred host. Free-living nematodes, like Caenorhabditis elegans, are often found in natural environments that contain terrestrial isopods and other invertebrates. Additionally, the C. elegans wild strain PB306 was isolated associated with the isopod Porcellio scaber. However, it is currently unclear if C. elegans is a phoront of terrestrial isopods, and if so, whether it is a specialist, generalist, or developmental stage-specific combination of both strategies. Because the relevant chemical stimuli might be secreted compounds or volatile odorants, we used different types of chemotaxis assays across diverse extractions of compounds or odorants to test whether C. elegans is attracted to P. scaber. We show that two different strains, the wild isolate PB306 and the laboratory-adapted strain N2, are not attracted to P. scaber during either the dauer or adult life stages. Our results indicate that C. elegans was not attracted to chemical compounds or volatile odorants from P. scaber, providing valuable empirical evidence to suggest that any associations between these two species are likely opportunistic rather than specific phoresy.
Salvador Ordonez - One of the best experts on this subject based on the ideXlab platform.
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gas phase acetone self condensation over unsupported and supported mg zr mixed oxides catalysts
Applied Catalysis B-environmental, 2013Co-Authors: Laura Faba, Eva Diaz, Salvador OrdonezAbstract:Abstract Acetone gas phase aldol condensation over bulk and carbon-supported Mg–Zr has been studied in this work. The condensation yields C6 (mainly mesityl oxide) and C9 (Phorones, isoPhorones and mesitylene) compounds, depending on reaction temperature and distribution of acid and basic sites. The influence of the temperature (323–723 K) was studied, higher C9 selectivities being obtained at higher temperatures (close to 50%). The influence of the concentration and the distribution of basic and acid sites was analyzed by comparing the results obtained with the bulk Mg–Zr and with Mg–Zr supported on non-microporous carbonaceous materials: high surface area graphites (HSAG) and carbon nanofibers (CNF). In addition, two different preparation methods (dry impregnation and co-precipitation) were tested. The performance of the catalysts is related to the distribution of acid and basic sites. Acid–basic pairs are needed for the acetone condensation, weak acidity for dehydration and cyclation reactions, and the strongest basicity for the condensation of mesityl oxide.