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Carlos R. Osorio - One of the best experts on this subject based on the ideXlab platform.
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Draft Genome Sequences of Photobacterium damselae subsp. piscicida SNW-8.1 and PP3, Two Fish-Isolated Strains Containing a Type III Secretion System.
Microbiology resource announcements, 2019Co-Authors: Saqr Abushattal, Ana Do Vale, Nuno M.s. Dos Santos, Ana Vences, Carlos R. OsorioAbstract:Here, we report the draft genome sequences of two strains of the fish pathogen Photobacterium damselae subsp. piscicida, isolated from Salmo salar (SNW-8.1) and Seriola quinqueradiata (PP3). The identification of a type III secretion system in the two genomes furthers our understanding of the pathobiology of this subspecies.
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Cytotoxin- and Chemotaxis-Genes Cooperate to Promote Adhesion of Photobacterium damselae subsp. damselae.
Frontiers in microbiology, 2018Co-Authors: Gisela Von Hoven, Carlos R. Osorio, Claudia Neukirch, Martina Meyenburg, Matthias Husmann, Ana Vences, Sabine Schmidt, Amable J. RivasAbstract:Photobacterium damselae subsp. damselae (Pdd) is an emerging pathogen of marine animals that sometimes causes serious infections in humans. Two related pore forming toxins, phobalysins P and C, and damselysin, a phospholipase D, confer strong virulence of Pdd in mice. Because infections by Pdd are typically caused following exposure of wounds to sea water we investigated how salinity impacts toxin activity, swimming, and association of Pdd with epithelial cells. These activities were low when bacteria were pre-cultured in media with 3.5% NaCl, the global average salinity of sea water. In contrast, lower salinity increased swimming of wild type Pdd peaking at 2% NaCl, hemolysis, and association with epithelial cells peaking at 1-1.5%. Previously, we have found that hemolysin genes enhance the association of Pdd with epithelial cells, but the underlying mechanisms have remained ill-defined. We here searched for potential links between hemolysin-production, chemotaxis and association of Pdd with target cells at varying salt concentrations. Unexpectedly, disruption of chemotaxis regulator cheA not only affected bacterial swimming and association with epithelial cells at intermediate to low salinity, but also reduced the production of plasmid-encoded phobalysin (PhlyP). The results thus reveal unforeseen links between chemotaxis regulators, a pore forming toxin and the association of a marine bacterium with target cells.
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Image_1_Cytotoxin- and Chemotaxis-Genes Cooperate to Promote Adhesion of Photobacterium damselae subsp. damselae.JPEG
2018Co-Authors: Gisela Von Hoven, Carlos R. Osorio, Claudia Neukirch, Martina Meyenburg, Matthias Husmann, Ana Vences, Sabine Schmidt, Amable J. RivasAbstract:Photobacterium damselae subsp. damselae (Pdd) is an emerging pathogen of marine animals that sometimes causes serious infections in humans. Two related pore forming toxins, phobalysins P and C, and damselysin, a phospholipase D, confer strong virulence of Pdd in mice. Because infections by Pdd are typically caused following exposure of wounds to sea water we investigated how salinity impacts toxin activity, swimming, and association of Pdd with epithelial cells. These activities were low when bacteria were pre-cultured in media with 3.5% NaCl, the global average salinity of sea water. In contrast, lower salinity increased swimming of wild type Pdd peaking at 2% NaCl, hemolysis, and association with epithelial cells peaking at 1–1.5%. Previously, we have found that hemolysin genes enhance the association of Pdd with epithelial cells, but the underlying mechanisms have remained ill-defined. We here searched for potential links between hemolysin-production, chemotaxis and association of Pdd with target cells at varying salt concentrations. Unexpectedly, disruption of chemotaxis regulator cheA not only affected bacterial swimming and association with epithelial cells at intermediate to low salinity, but also reduced the production of plasmid-encoded phobalysin (PhlyP). The results thus reveal unforeseen links between chemotaxis regulators, a pore forming toxin and the association of a marine bacterium with target cells.
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The Apoptogenic Toxin AIP56 Is Secreted by the Type II Secretion System of Photobacterium damselae subsp. piscicida
Toxins, 2017Co-Authors: Ana Do Vale, Cassilda Pereira, Carlos R. Osorio, Nuno M.s. Dos SantosAbstract:AIP56 (apoptosis-inducing protein of 56 kDa) is a key virulence factor of Photobacterium damselae subsp. piscicida (Phdp), the causative agent of a septicaemia affecting warm water marine fish species. Phdp-associated pathology is triggered by AIP56, a short trip AB toxin with a metalloprotease A domain that cleaves the p65 subunit of NF-κB, an evolutionarily conserved transcription factor that regulates the expression of inflammatory and anti-apoptotic genes and plays a central role in host responses to infection. During infection by Phdp, AIP56 is systemically disseminated and induces apoptosis of macrophages and neutrophils, compromising the host phagocytic defence and contributing to the genesis of pathology. Although it is well established that the secretion of AIP56 is crucial for Phdp pathogenicity, the protein secretion systems operating in Phdp and the mechanism responsible for the extracellular release of the toxin remain unknown. Here, we report that Phdp encodes a type II secretion system (T2SS) and show that mutation of the EpsL component of this system impairs AIP56 secretion. This work demonstrates that Phdp has a functional T2SS that mediates secretion of its key virulence factor AIP56.
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Chromosome-Encoded Hemolysin, Phospholipase, and Collagenase in Plasmidless Isolates of Photobacterium damselae subsp. damselae Contribute to Virulence for Fish.
Applied and environmental microbiology, 2017Co-Authors: Ana Vences, Manuel L. Lemos, Amable J. Rivas, Matthias Husmann, Carlos R. OsorioAbstract:Photobacterium damselae subsp. damselae is a pathogen of marine animals including fish of importance in aquaculture. The virulence plasmid pPHDD1, characteristic of highly hemolytic isolates, encodes the hemolysins damselysin (Dly) and phobalysin (PhlyP). Strains lacking pPHDD1 constitute the vast majority of the isolates from fish outbreaks, but genetic studies to identify virulence factors in plasmidless strains are scarce. Here we show that the chromosome-I encoded hemolysin PhlyC plays a role in virulence and cell toxicity in pPHDD1-negative isolates of this pathogen. By combining the analysis of whole genomes and of gene deletion mutants, we identified two hitherto uncharacterized chromosomal loci encoding a phospholipase (PlpV) and a collagenase (ColP), respectively. PlpV was ubiquitous in the subspecies and exerted hemolytic activity against fish erythrocytes which was enhanced in the presence of lecithin. ColP was restricted to a fraction of the isolates, and was responsible for the collagen-degrading activity in this subspecies. Consistent with the presence of signal peptides in PlpV and ColP sequences, mutants for the type II secretion system (T2SS) genes epsL and pilD exhibited impairment in the production of phospholipase and collagenase activities. Sea bass virulence experiments and cell culture assays demonstrated a major contribution of PhlyC and PlpV to virulence and toxicity. IMPORTANCE This study constitutes the first genetic and genomic analysis of plasmidless strains of the emerging pathogen in marine aquaculture Photobacterium damselae subsp. damselae . To date, studies on the genetic basis of virulence were restricted to the pPHDD1 plasmid-encoded toxins Dly and PhlyP. However, the vast majority of the recent isolates of this pathogen from fish farm outbreaks lack this plasmid. Here we demonstrate that the plasmidless strains produce two hitherto uncharacterized ubiquitous toxins encoded in chromosome I: the hemolysin PhlyC and the phospholipase PlpV. We report a main role of these two toxins in fish virulence and in cell toxicity. Our results constitute the basis for a better understanding of the virulence of a widespread marine pathogen.
Miguel A. Moriñigo - One of the best experts on this subject based on the ideXlab platform.
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Transcription of IVIAT and Virulence Genes in Photobacterium damselae subsp. piscicida Infecting Solea senegalensis
Microorganisms, 2018Co-Authors: J.a. Núñez-díaz, Ana Do Vale, Miguel A. Moriñigo, Milena Fumanal, C. Fernández-díaz, M.c. BalebonaAbstract:Photobacterium damselae subsp. piscicida (Phdp) is responsible for disease outbreaks in marine aquaculture worldwide. Solea senegalensis, a valuable fish species for aquaculture in the south of Europe, is frequently affected by this pathogen. It is well established that bacteria respond to environmental signals and, in the case of pathogens, this ability may determine the outcome of their interaction with the host. Determination of gene expression under in vivo conditions constitutes a valuable tool in the assessment of microbial pathogenesis. Considering that different hosts may represent different environments for the pathogen, expression of Phdp virulence and in vivo induced antigen (IVIAT) genes during S. senegalensis infection has been determined in the present work. Increased transcription of genes encoding proteins involved in iron acquisition (Irp1, Irp2, HutB and HutD), oxidative stress defence (AhpC and Sod), adhesion (PDP_0080), toxins (AIP56) and metabolism (Impdh, Shmt and AlaRS) were detected in Phdp infecting S. senegalensis head kidney or liver. The highest increases corresponded to genes involved in survival under iron limiting conditions and oxidative stress, indicating their essential role during infection of sole. Results obtained give insight into Phdp virulence strategies and contribute to the identification of promising targets for the control of photobacteriosis.
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Superoxide dismutase and catalase activities in Photobacterium damselae ssp. piscicida.
Journal of fish diseases, 2006Co-Authors: Patricia Díaz-rosales, Miguel A. Moriñigo, S. Arijo, M Chabrillón, E. Martinez-manzanares, M.c. BalebonaAbstract:The ability of a set of Photobacterium damselae ssp. piscicida strains isolated from different fish species to produce different superoxide dismutase (SOD) and catalase enzymes was determined. Unlike other bacterial pathogens, P. damselae ssp. piscicida is not able to produce different isoforms of SOD or catalase containing different metal cofactors when cultured under oxidative stress induced by hydrogen peroxide or methyl viologen, or under iron depleted conditions. However, iron content of the growth medium influenced the levels of SOD and catalase activity in cells, these levels decreasing with iron availability of the medium. Comparison of virulent and non-virulent strains of P. damselae ssp. piscicida showed similar contents of SOD, but higher levels of catalase were detected in cells of the virulent strain. Incubation of bacteria with sole, Solea senegalensis (Kaup), phagocytes has shown that survival rates range from 19% to 62%, these rates being higher for the virulent strain. The increased levels of catalase activity detected in the virulent strain indicates a possible role for this enzyme in bacterial survival.
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Effectiveness of a divalent vaccine for sole, Solea senegalensis (Kaup), against Vibrio harveyi and Photobacterium damselae subsp. piscicida
Journal of fish diseases, 2005Co-Authors: S. Arijo, Alicia E. Toranzo, Beatriz Magariños, Patricia Díaz-rosales, M Chabrillón, E. Martinez-manzanares, R. M. Rico, Mª. Carmen Balebona, Miguel A. MoriñigoAbstract:The protection of cultured sole, Solea senegalensis, against Vibrio harveyi and Photobacterium damselae subsp. piscicida was evaluated following the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms and administered by the immersion route. Two prolonged immersions of 5-10 g fish in the divalent bacterin at a 1-month interval gave high levels of protection similar to those obtained when the respective monovalent vaccines were administered by the intraperitoneal route [relative percentage of survival (RPS) values >70%], which indicates that the former procedure can be a useful strategy with small fish. The high protection afforded by the divalent vaccine in sole lasted for 4 months after which the RPS values against both pathogens decreased significantly.
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Effectiveness of a divalent vaccine for gilt- head sea bream (Sparus aurata) against Vibrio alginolyticus and Photobacterium damselae subsp. piscicida
2002Co-Authors: Miguel A. Moriñigo, Jesús L. Romalde, Beatriz Magariños, S. Arijo, Carmen BalebonaAbstract:The protection of gilt-head sea bream (Sparus aurata) against two pathogens such as Vibrio alginolyticus and Photobacterium damselae subsp. piscicida was evaluated by the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms. This vaccine, administered by immersion protected to fish of 0.1 g and 1 g being the relative percent survival (RPS) higher than 70% in all experiments.
Alicia E. Toranzo - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida.
Biological research, 2007Co-Authors: Monique Mancuso, Renata Zaccone, Alicia E. Toranzo, Ruben Avendaño-herrera, Beatriz MagariñosAbstract:This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERICPCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections.
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Variation in 16S-23S rRNA Intergenic Spacer Regions in Photobacterium damselae: a Mosaic-Like Structure
Applied and environmental microbiology, 2005Co-Authors: Carlos R. Osorio, Jesús L. Romalde, Matthew D. Collins, Alicia E. ToranzoAbstract:Phenotypically, Photobacterium damselae subsp. piscicida and P. damselae subsp. damselae are easily distinguished. However, their 16S rRNA gene sequences are identical, and attempts to discriminate these two subspecies by molecular tools are hampered by their high level of DNA-DNA similarity. The 16S-23S rRNA internal transcribed spacers (ITS) were sequenced in two strains of Photobacterium damselae subsp. piscicida and two strains of P. damselae subsp. damselae to determine the level of molecular diversity in this DNA region. A total of 17 different ITS variants, ranging from 803 to 296 bp were found, some of which were subspecies or strain specific. The largest ITS contained four tRNA genes (tDNAs) coding for tRNA(Glu(UUC)), tRNA(Lys(UUU)), tRNA(Val(UAC)), and tRNA(Ala(GGC)). Five amplicons contained tRNA(Glu(UUC)) combined with two additional tRNA genes, including tRNA(Lys(UUU)), tRNA(Val(UAC)), or tRNA(Ala(UGC)). Five amplicons contained tRNA(Ile(GAU)) and tRNA(Ala(UGC)). Two amplicons contained tRNA(Glu(UUC)) and tRNA(Ala(UGC)). Two different isoacceptor tRNA(Ala) genes (GGC and UGC anticodons) were found. The five smallest amplicons contained no tRNA genes. The tRNA-gene combinations tRNA(Glu(UUC))-tRNA(Val(UAC))-tRNA(Ala(UGC)) and tRNA(Glu(UUC))-tRNA(Ala(UGC)) have not been previously reported in bacterial ITS regions. The number of copies of the ribosomal operon (rrn) in the P. damselae chromosome ranged from at least 9 to 12. For ITS variants coexisting in two strains of different subspecies or in strains of the same subspecies, nucleotide substitution percentages ranged from 0 to 2%. The main source of variation between ITS variants was due to different combinations of DNA sequence blocks, constituting a mosaic-like structure.
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Effectiveness of a divalent vaccine for sole, Solea senegalensis (Kaup), against Vibrio harveyi and Photobacterium damselae subsp. piscicida
Journal of fish diseases, 2005Co-Authors: S. Arijo, Alicia E. Toranzo, Beatriz Magariños, Patricia Díaz-rosales, M Chabrillón, E. Martinez-manzanares, R. M. Rico, Mª. Carmen Balebona, Miguel A. MoriñigoAbstract:The protection of cultured sole, Solea senegalensis, against Vibrio harveyi and Photobacterium damselae subsp. piscicida was evaluated following the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms and administered by the immersion route. Two prolonged immersions of 5-10 g fish in the divalent bacterin at a 1-month interval gave high levels of protection similar to those obtained when the respective monovalent vaccines were administered by the intraperitoneal route [relative percentage of survival (RPS) values >70%], which indicates that the former procedure can be a useful strategy with small fish. The high protection afforded by the divalent vaccine in sole lasted for 4 months after which the RPS values against both pathogens decreased significantly.
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Binding of haemin by the fish pathogen Photobacterium damselae subsp. piscicida.
Diseases of aquatic organisms, 2002Co-Authors: Ana Do Vale, Manuel L. Lemos, Anthony E. Ellis, Jesús L. Romalde, Beatriz Magariños, Alicia E. ToranzoAbstract:Whole cells of virulent (DI 21 and B 51) and avirulent (ATCC 29690 and EPOY 8803-II) strains of Photobacterium damselae subsp. piscicida, grown under iron-supplemented or iron-restricted conditions, were able to bind haemin. Iron limitation resulted in an increased binding of haemin by DI 21, B 51 and ATCC 29690 cells but did not affect the haemin-binding ability of the EPOY 8803-II cells. Proteinase K treatment of whole cells markedly reduced the binding of haemin, indicating that protein receptors located at the cell surface are involved in the binding. This was confirmed by the observation that isolated total as well as outer membrane proteins from all the strains, regardless of the iron levels of the media, were able to bind haemin, with the outer membranes showing the strongest binding. Haemin binding by membrane protein extracts was not affected by heat treatment but was almost completely abolished by Proteinase K treatment, suggesting the presence of thermostable protein receptors for haemin. The capsular polysaccharide also appears to play a minor role in binding of haemin. It was concluded that constitutive as well as inducible mechanisms of haemin binding occur in P. damselae subsp. piscicida. These mechanisms would rely mainly upon the direct interaction between the haemin molecules and surface-exposed outer membrane protein receptors.
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Existence of two geographically-linked clonal lineages in the bacterial fish pathogen Photobacterium damselae subsp. piscicida evidenced by random amplified polymorphic DNA analysis.
Epidemiology and infection, 2000Co-Authors: Beatriz Magariños, Juan L. Barja, Alicia E. Toranzo, Jesús L. RomaldeAbstract:In this work, we applied the random amplified polymorphic DNA (RAPD) technique to evaluate the genetic diversity in Photobacterium damselae subsp. piscicida (formerly Pasteurella piscicida), an important pathogen for different marine fish. Regardless of the oligonucleotide primer employed, the 29 isolates of Ph. damselae subsp. piscicida tested were separated into two groups, the RAPD-PCR analysis differentiated the European strains from the Japanese strains. The similarity between both groups estimated on the basis of the Dice coefficient was 75-80%. These results show that European and Japanese isolates of Ph. damselae subsp. piscicida, regardless of their host fish species, belong to two different clonal lineages. Our findings also indicate that RAPD profiling constitutes a useful tool for epidemiological studies of this fish pathogen.
Beatriz Magariños - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida.
Biological research, 2007Co-Authors: Monique Mancuso, Renata Zaccone, Alicia E. Toranzo, Ruben Avendaño-herrera, Beatriz MagariñosAbstract:This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERICPCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections.
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Effectiveness of a divalent vaccine for sole, Solea senegalensis (Kaup), against Vibrio harveyi and Photobacterium damselae subsp. piscicida
Journal of fish diseases, 2005Co-Authors: S. Arijo, Alicia E. Toranzo, Beatriz Magariños, Patricia Díaz-rosales, M Chabrillón, E. Martinez-manzanares, R. M. Rico, Mª. Carmen Balebona, Miguel A. MoriñigoAbstract:The protection of cultured sole, Solea senegalensis, against Vibrio harveyi and Photobacterium damselae subsp. piscicida was evaluated following the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms and administered by the immersion route. Two prolonged immersions of 5-10 g fish in the divalent bacterin at a 1-month interval gave high levels of protection similar to those obtained when the respective monovalent vaccines were administered by the intraperitoneal route [relative percentage of survival (RPS) values >70%], which indicates that the former procedure can be a useful strategy with small fish. The high protection afforded by the divalent vaccine in sole lasted for 4 months after which the RPS values against both pathogens decreased significantly.
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Occurrence of Photobacterium damselae subsp. damselae in bivavlve molluscs from Northwest Spain
Bulletin of The European Association of Fish Pathologists, 2003Co-Authors: Antonio Lozano-leon, Carlos R. Osorio, S. Nunez, Jaime Martinez-urtaza, Beatriz MagariñosAbstract:Photobacterium damselae subsp. damselae has been recognized as pathogen for poikilotherm and homeotherm organims. In this study we describe for the first time the isolation of this microorganism from Mytilus galloprovincialis in harvesting areas situated in the northwestern coast of Spain. The identity of the isolates as Ph. damselae subsp. damselae was confirmed by biochemical tests and multiplex PCR technique. Interestingly, the absence of gas production from glucose in the bivalve mollusc strains indicated that the importance of this biochemical trait for the presumptive identification of this microorganism need to be reevaluated. The family Vibrionaceae comprises bacteria inhabiting aquatic enviroments, especially marine and estuarine waters, where they are frecuently associated with organims ranging from plankton to fish. Currently, this family includes, among others, the marine genera Vibrio (Baumann et al., 1984) and Photobacterium (Baumann & Baumann, 1981). Within the genus Photobacterium, Ph. damselae subsp. damselae (formerly Vibrio damsela) is an halophilic bacterium that has been described as the causative agent of wound infections and fatal disease in a variety of marine animals and human (Grimes et al, 1984, Mc Garey et al, 1990). In fact, since the first isolation of Ph.damselae subsp. damselae as the causative organism of skin ulcers on the damselfish Chromis punctipinnis (Love et al., 1981), this bacterium has also been recognized as a pathogen for the others fish, such as turbot, seabream, seabass, yellowtail and sharks, reptiles, molluscs, and crustacean species (
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Binding of haemin by the fish pathogen Photobacterium damselae subsp. piscicida.
Diseases of aquatic organisms, 2002Co-Authors: Ana Do Vale, Manuel L. Lemos, Anthony E. Ellis, Jesús L. Romalde, Beatriz Magariños, Alicia E. ToranzoAbstract:Whole cells of virulent (DI 21 and B 51) and avirulent (ATCC 29690 and EPOY 8803-II) strains of Photobacterium damselae subsp. piscicida, grown under iron-supplemented or iron-restricted conditions, were able to bind haemin. Iron limitation resulted in an increased binding of haemin by DI 21, B 51 and ATCC 29690 cells but did not affect the haemin-binding ability of the EPOY 8803-II cells. Proteinase K treatment of whole cells markedly reduced the binding of haemin, indicating that protein receptors located at the cell surface are involved in the binding. This was confirmed by the observation that isolated total as well as outer membrane proteins from all the strains, regardless of the iron levels of the media, were able to bind haemin, with the outer membranes showing the strongest binding. Haemin binding by membrane protein extracts was not affected by heat treatment but was almost completely abolished by Proteinase K treatment, suggesting the presence of thermostable protein receptors for haemin. The capsular polysaccharide also appears to play a minor role in binding of haemin. It was concluded that constitutive as well as inducible mechanisms of haemin binding occur in P. damselae subsp. piscicida. These mechanisms would rely mainly upon the direct interaction between the haemin molecules and surface-exposed outer membrane protein receptors.
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Effectiveness of a divalent vaccine for gilt- head sea bream (Sparus aurata) against Vibrio alginolyticus and Photobacterium damselae subsp. piscicida
2002Co-Authors: Miguel A. Moriñigo, Jesús L. Romalde, Beatriz Magariños, S. Arijo, Carmen BalebonaAbstract:The protection of gilt-head sea bream (Sparus aurata) against two pathogens such as Vibrio alginolyticus and Photobacterium damselae subsp. piscicida was evaluated by the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms. This vaccine, administered by immersion protected to fish of 0.1 g and 1 g being the relative percent survival (RPS) higher than 70% in all experiments.
S. Arijo - One of the best experts on this subject based on the ideXlab platform.
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Superoxide dismutase and catalase activities in Photobacterium damselae ssp. piscicida.
Journal of fish diseases, 2006Co-Authors: Patricia Díaz-rosales, Miguel A. Moriñigo, S. Arijo, M Chabrillón, E. Martinez-manzanares, M.c. BalebonaAbstract:The ability of a set of Photobacterium damselae ssp. piscicida strains isolated from different fish species to produce different superoxide dismutase (SOD) and catalase enzymes was determined. Unlike other bacterial pathogens, P. damselae ssp. piscicida is not able to produce different isoforms of SOD or catalase containing different metal cofactors when cultured under oxidative stress induced by hydrogen peroxide or methyl viologen, or under iron depleted conditions. However, iron content of the growth medium influenced the levels of SOD and catalase activity in cells, these levels decreasing with iron availability of the medium. Comparison of virulent and non-virulent strains of P. damselae ssp. piscicida showed similar contents of SOD, but higher levels of catalase were detected in cells of the virulent strain. Incubation of bacteria with sole, Solea senegalensis (Kaup), phagocytes has shown that survival rates range from 19% to 62%, these rates being higher for the virulent strain. The increased levels of catalase activity detected in the virulent strain indicates a possible role for this enzyme in bacterial survival.
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Effectiveness of a divalent vaccine for sole, Solea senegalensis (Kaup), against Vibrio harveyi and Photobacterium damselae subsp. piscicida
Journal of fish diseases, 2005Co-Authors: S. Arijo, Alicia E. Toranzo, Beatriz Magariños, Patricia Díaz-rosales, M Chabrillón, E. Martinez-manzanares, R. M. Rico, Mª. Carmen Balebona, Miguel A. MoriñigoAbstract:The protection of cultured sole, Solea senegalensis, against Vibrio harveyi and Photobacterium damselae subsp. piscicida was evaluated following the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms and administered by the immersion route. Two prolonged immersions of 5-10 g fish in the divalent bacterin at a 1-month interval gave high levels of protection similar to those obtained when the respective monovalent vaccines were administered by the intraperitoneal route [relative percentage of survival (RPS) values >70%], which indicates that the former procedure can be a useful strategy with small fish. The high protection afforded by the divalent vaccine in sole lasted for 4 months after which the RPS values against both pathogens decreased significantly.
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Effectiveness of a divalent vaccine for gilt- head sea bream (Sparus aurata) against Vibrio alginolyticus and Photobacterium damselae subsp. piscicida
2002Co-Authors: Miguel A. Moriñigo, Jesús L. Romalde, Beatriz Magariños, S. Arijo, Carmen BalebonaAbstract:The protection of gilt-head sea bream (Sparus aurata) against two pathogens such as Vibrio alginolyticus and Photobacterium damselae subsp. piscicida was evaluated by the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms. This vaccine, administered by immersion protected to fish of 0.1 g and 1 g being the relative percent survival (RPS) higher than 70% in all experiments.