The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Fulton T. Crews - One of the best experts on this subject based on the ideXlab platform.
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Adolescent intermittent ethanol (AIE) exposure leads to global reductions of choline acetyltransferase (ChAT)-immunopositive cells in the young adult brain.
2014Co-Authors: Ryan P. Vetreno, Margaret Broadwater, Wen Liu, Linda P. Spear, Fulton T. CrewsAbstract:(A) Profile counts revealed that AIE reduced ChAT+IR in the medial septum/vertical limb of the diagonal band (Ch1/Ch2 [29%±4%]) and horizontal limb of the diagonal band/nucleus basalis magnocellularis (Ch3/Ch4 [36%±6%]) of the basal forebrain, relative to controls (CONs). Representative photomicrographs of ChAT+IR cells in Ch1/Ch2 of CON- and AIE-exposed basal forebrain. (B) Profile counts found that AIE reduced ChAT+IR in the pedunculopontine nucleus (Ch5; 21%±3%) and the laterodorsal tegmental nucleus (Ch6; 17%±5%) of the brainstem, relative to CONs. Representative photomicrographs of ChAT+IR cells in Ch5 of the brainstem from CON- and AIE-exposed animals. (C) ChAT pixel density (×1000 pixels/mm2) was reduced by 34% (±7%) in the medial habenula (Ch7) of AIE-exposed animals, relative to CONs. Representative photomicrographs of ChAT+IR cells in Ch7 from CON- and AIE-exposed animals. (D) AIE reduced ChAT pixel density (×1000 pixels/mm2) by 27% (±4%) in the interpeduncular nucleus (IPn), relative to CONs. Representative photomicrographs of ChAT immunoreactivity cells in the IPn from CON- and AIE-exposed animals. Data are presented as mean ± SEM. * indicates p
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Choline acetyltransferase (ChAT) expression is persistently decreased in the basal forebrain following adolescent intermittent ethanol (AIE) exposure.
2014Co-Authors: Ryan P. Vetreno, Margaret Broadwater, Wen Liu, Linda P. Spear, Fulton T. CrewsAbstract:(A) Profile counts revealed a 24% (±7%) decrease of ChAT+IR cells in the medial septum/vertical limb of the diagonal band (Ch1/Ch2) of adolescent rats (P56) 24 hours following the conclusion of AIE that persisted into adulthood (P220 [19%±3%]), relative to CONs. Representative photomicrographs of ChAT+IR cells in Ch1/Ch2 of CON- and AIE-exposed basal forebrain samples from P220 subjects. (B) Quantification of ChAT immunoreactivity in the horizontal limb of the diagonal band/nucleus basalis magnocellularis (Ch3/Ch4) revealed that AIE reduced ChAT expression by 31% (±4%) at P56 that persisted to P220 (19%±3%), relative to CONs. Representative photomicrographs of ChAT+IR cells in Ch3/Ch4 of CON- and AIE-exposed basal forebrain samples from P220 subjects. Data are presented as mean ± SEM. * indicates p
Marc Del Bigio - One of the best experts on this subject based on the ideXlab platform.
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Additional file 2: of DNA methylation and histone post-translational modification stability in post-mortem brain tissue
2019Co-Authors: Jessica Jarmasz, Hannah Stirton, James Davie, Marc Del BigioAbstract:Figure S1. Examples of peptide blocking immunohistochemistry for histone post-translational modifications in neonatal pig neocortex. Top row photomicrographs show anti-histone H3 trimethylated at lysine 4 (H3K4me3). H3K4me3 was blocked by its own peptide (K4me3), but also partially blocked by other less specific peptides (K4me, K4me2, K9me3). Bottom row photomicrographs show anti-H3K27ac. It was blocked only by its own peptide (K27ac) and not by others (H4K5ac; others not shown). Images all taken at Ă 400 magnification. DAB detection of antibody (brown) and hematoxylin counterstain (blue). Figure S2. Complete dot blot peptide results. A small quantity of peptide was directly pipetted onto nitrocellulose membrane. The membrane was blocked and incubated with the antibody overnight. (PDF 4535 kb
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Additional file 13: of DNA methylation and histone post-translational modification stability in post-mortem brain tissue
2019Co-Authors: Jessica Jarmasz, Hannah Stirton, James Davie, Marc Del BigioAbstract:Figure S1. Standard scale images used for grading the proportion of immunoreactive nuclei. Any degree of brown was considered positive. Photomicrographs show: 4 - ~100% positive; 3 - ~75% positive; 2 - ~50% positive; 1
Ryan P. Vetreno - One of the best experts on this subject based on the ideXlab platform.
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Adolescent intermittent ethanol (AIE) exposure leads to global reductions of choline acetyltransferase (ChAT)-immunopositive cells in the young adult brain.
2014Co-Authors: Ryan P. Vetreno, Margaret Broadwater, Wen Liu, Linda P. Spear, Fulton T. CrewsAbstract:(A) Profile counts revealed that AIE reduced ChAT+IR in the medial septum/vertical limb of the diagonal band (Ch1/Ch2 [29%±4%]) and horizontal limb of the diagonal band/nucleus basalis magnocellularis (Ch3/Ch4 [36%±6%]) of the basal forebrain, relative to controls (CONs). Representative photomicrographs of ChAT+IR cells in Ch1/Ch2 of CON- and AIE-exposed basal forebrain. (B) Profile counts found that AIE reduced ChAT+IR in the pedunculopontine nucleus (Ch5; 21%±3%) and the laterodorsal tegmental nucleus (Ch6; 17%±5%) of the brainstem, relative to CONs. Representative photomicrographs of ChAT+IR cells in Ch5 of the brainstem from CON- and AIE-exposed animals. (C) ChAT pixel density (×1000 pixels/mm2) was reduced by 34% (±7%) in the medial habenula (Ch7) of AIE-exposed animals, relative to CONs. Representative photomicrographs of ChAT+IR cells in Ch7 from CON- and AIE-exposed animals. (D) AIE reduced ChAT pixel density (×1000 pixels/mm2) by 27% (±4%) in the interpeduncular nucleus (IPn), relative to CONs. Representative photomicrographs of ChAT immunoreactivity cells in the IPn from CON- and AIE-exposed animals. Data are presented as mean ± SEM. * indicates p
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Choline acetyltransferase (ChAT) expression is persistently decreased in the basal forebrain following adolescent intermittent ethanol (AIE) exposure.
2014Co-Authors: Ryan P. Vetreno, Margaret Broadwater, Wen Liu, Linda P. Spear, Fulton T. CrewsAbstract:(A) Profile counts revealed a 24% (±7%) decrease of ChAT+IR cells in the medial septum/vertical limb of the diagonal band (Ch1/Ch2) of adolescent rats (P56) 24 hours following the conclusion of AIE that persisted into adulthood (P220 [19%±3%]), relative to CONs. Representative photomicrographs of ChAT+IR cells in Ch1/Ch2 of CON- and AIE-exposed basal forebrain samples from P220 subjects. (B) Quantification of ChAT immunoreactivity in the horizontal limb of the diagonal band/nucleus basalis magnocellularis (Ch3/Ch4) revealed that AIE reduced ChAT expression by 31% (±4%) at P56 that persisted to P220 (19%±3%), relative to CONs. Representative photomicrographs of ChAT+IR cells in Ch3/Ch4 of CON- and AIE-exposed basal forebrain samples from P220 subjects. Data are presented as mean ± SEM. * indicates p
Jessica Jarmasz - One of the best experts on this subject based on the ideXlab platform.
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Additional file 2: of DNA methylation and histone post-translational modification stability in post-mortem brain tissue
2019Co-Authors: Jessica Jarmasz, Hannah Stirton, James Davie, Marc Del BigioAbstract:Figure S1. Examples of peptide blocking immunohistochemistry for histone post-translational modifications in neonatal pig neocortex. Top row photomicrographs show anti-histone H3 trimethylated at lysine 4 (H3K4me3). H3K4me3 was blocked by its own peptide (K4me3), but also partially blocked by other less specific peptides (K4me, K4me2, K9me3). Bottom row photomicrographs show anti-H3K27ac. It was blocked only by its own peptide (K27ac) and not by others (H4K5ac; others not shown). Images all taken at Ă 400 magnification. DAB detection of antibody (brown) and hematoxylin counterstain (blue). Figure S2. Complete dot blot peptide results. A small quantity of peptide was directly pipetted onto nitrocellulose membrane. The membrane was blocked and incubated with the antibody overnight. (PDF 4535 kb
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Additional file 13: of DNA methylation and histone post-translational modification stability in post-mortem brain tissue
2019Co-Authors: Jessica Jarmasz, Hannah Stirton, James Davie, Marc Del BigioAbstract:Figure S1. Standard scale images used for grading the proportion of immunoreactive nuclei. Any degree of brown was considered positive. Photomicrographs show: 4 - ~100% positive; 3 - ~75% positive; 2 - ~50% positive; 1
Wenxian Zeng - One of the best experts on this subject based on the ideXlab platform.
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Photomicrographs of the post-thaw rabbit spermatozoa.
2017Co-Authors: Zhendong Zhu, Zhanjun Ren, Xiaoteng Fan, Yang Pan, Chuanying Pan, Anmin Lei, Wenxian ZengAbstract:Images(C, D) obtained under phase contrast microscopy. (A) Image obtained by FITC-labeled peanut agglutinin staining, Images (A) and (C) are from the same field. Red arrow indicates intact acrosomes, spermatozoa with intensively bright fluorescence of the acrosomal cap which were indicated by an intact outer acrosomal membrane; white arrow indicates damaged acrosome, spermatozoa with no fluorescence which were indicated by a complete loss of the outer acrosomal membrane and was determined under a phase contrast illumination system; blue arrow indicates partially damaged acrosome, spermatozoa with disrupted fluorescence of the acrosomal cap which were indicated by partial disruption of the acrosomal membrane; (B) Image obtained by SYBR-14 plus propidium ioide (PI) staining. Images (B) and (D) were from the same field. Blue arrow indicates membrane integrity, which were stained green with SYBR-14 but not with PI; white arrow indicates membrane damaged, which were stained red with PI but not with SYBR-14. Scale bars represent 30 μm.
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Photomicrographs of the post-thaw rabbit spermatozoa.
2016Co-Authors: Zhendong Zhu, Xiaoteng Fan, Nan Zhang, Chuning Fan, Pengfei Zhang, Wenxian ZengAbstract:(A, C) Images obtained by phase contrast microscope. (A) Image obtained by FITC-labeled peanut agglutinin staining, Images (A) and (B) were from the same field. White arrow indicates intact acrosomes; blue arrow indicates partially damaged acrosome; red arrow indicates damaged acrosome. (D) Image obtained by SYBR-14 plus propidium ioide (PI) staining. Images (C) and (D) were from the same field. red arrow indicates membrane integrity; blue arrow indicates membrane damaged; white arrow indicates membrane damaged slightly. Scale bars represent 30 μm.