The Experts below are selected from a list of 1050 Experts worldwide ranked by ideXlab platform
Christina M Holzapfel - One of the best experts on this subject based on the ideXlab platform.
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natural variation and genetics of Photoperiodism in wyeomyia smithii
Advances in Genetics, 2017Co-Authors: William E Bradshaw, Christina M HolzapfelAbstract:Seasonal change in the temperate and polar regions of Earth determines how the world looks around us and, in fact, how we live our day-to-day lives. For biological organisms, seasonal change typically involves complex physiological and metabolic reorganization, the majority of which is regulated by Photoperiodism. Photoperiodism is the ability of animals and plants to use day length or night length, resulting in life-historical transformations, including seasonal development, migration, reproduction, and dormancy. Seasonal timing determines not only survival and reproductive success but also the structure and organization of complex communities and, ultimately, the biomes of Earth. Herein, a small mosquito, Wyeomyia smithii, that lives only in the water-filled leaves of a carnivorous plant over a wide geographic range, is used to explore the genetic and evolutionary basis of Photoperiodism. Photoperiodism in W. smithii is considered in the context of its historical biogeography in nature to examine the startling finding that recent rapid climate change can drive genetic change in plants and animals at break-neck speed, and to challenge the ponderous 80+ year search for connections between daily and seasonal time-keeping mechanisms. Finally, a model is proposed that reconciles the seemingly disparate 24-h daily clock driven by the invariant rotation of Earth about its axis with the evolutionarily flexible seasonal timer orchestrated by variable seasonality driven by the rotation of Earth about the Sun.
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complications of complexity integrating environmental genetic and hormonal control of insect diapause
Trends in Genetics, 2009Co-Authors: Kevin J Emerson, William E Bradshaw, Christina M HolzapfelAbstract:Understanding gene interaction and pleiotropy are long-standing goals of developmental and evolutionary biology. We examine the genetic control of diapause in insects and show how the failure to recognize the difference between modular and gene pleiotropy has confounded our understanding of the genetic basis of this important phenotype. This has led to complications in understanding the role of the circadian clock in the control of diapause in Drosophila and other insects. We emphasize three successive modules – each containing functionally related genes – that lead to diapause: Photoperiodism, hormonal events and diapause itself. Understanding the genetic basis for environmental control of diapause has wider implications for evolutionary response to rapid climate change and for the opportunity to observe evolutionary change in contemporary time.
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Evolution of photoperiodic time measurement is independent of the circadian clock in the pitcher-plant mosquito, Wyeomyia smithii
Journal of Comparative Physiology A, 2009Co-Authors: K J Emerson, William E Bradshaw, Sabrina J. Dake, Christina M HolzapfelAbstract:For over 70 years, researchers have debated whether the ability to use day length as a cue for the timing of seasonal events (Photoperiodism) is related to the endogenous circadian clock that regulates the timing of daily events. Models of Photoperiodism include two components: (1) a photoperiodic timer that measures the length of the day, and (2) a photoperiodic counter that elicits the downstream photoperiodic response after a threshold number of days has been counted. Herein, we show that there is no geographical pattern of genetic association between the expression of the circadian clock and the photoperiodic timer or counter. We conclude that the photoperiodic timer and counter have evolved independently of the circadian clock in the pitcher-plant mosquito Wyeomyia smithii and hence, the evolutionary modification of Photoperiodism throughout the range of W. smithii has not been causally mediated by a corresponding evolution of the circadian clock.
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quantitative trait loci associated with photoperiodic response and stage of diapause in the pitcher plant mosquito wyeomyia smithii
Genetics, 2007Co-Authors: Derrick Mathias, William E Bradshaw, Lucien Jacky, Christina M HolzapfelAbstract:A wide variety of temperate animals rely on length of day (Photoperiodism) to anticipate and prepare for changing seasons by regulating the timing of development, reproduction, dormancy, and migration. Although the molecular basis of circadian rhythms regulating daily activities is well defined, the molecular basis for the photoperiodic regulation of seasonal activities is largely unknown. We use geographic variation in the photoperiodic control of diapause in the pitcher-plant mosquito Wyeomyia smithii to create the first QTL map of Photoperiodism in any animal. For critical photoperiod (CPP), we detect QTL that are unique, a QTL that is sex linked, QTL that overlap with QTL for stage of diapause (SOD), and a QTL that interacts epistatically with the circadian rhythm gene, timeless. Results presented here confirm earlier studies concluding that CPP is under directional selection over the climatic gradient of North America and that the evolution of CPP is genetically correlated with SOD. Despite epistasis between timeless and a QTL for CPP, timeless is not located within any detectable QTL, indicating that it plays an ancillary role in the evolution of Photoperiodism in W. smithii. Finally, we highlight one region of the genome that includes loci contributing to CPP, SOD, and hormonal regulation of development.
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Evolution of Animal Photoperiodism
Annual Review of Ecology Evolution and Systematics, 2007Co-Authors: William E Bradshaw, Christina M HolzapfelAbstract:AbstractPhotoperiodism is the ability of organisms to assess and use the day length as an anticipatory cue to time seasonal events in their life histories. Photoperiodism is especially important in initiating physiological and developmental processes that are typically irrevocable and that culminate at a future time or at a distant place; the further away in space or time, the more likely a seasonal event is initiated by photoperiod. The pervasiveness of Photoperiodism across broad taxa, from rotifers to rodents, and the predictable changes of photoperiodic response with geography identify it as a central component of fitness in temperate and polar seasonal environments. Consequently, the role of day length cannot be disregarded when evaluating the mechanisms underlying life-historical events, range expansions, invasions of novel species, and response to climate change among animals in the temperate and polar regions of the world.
Sakiko Shiga - One of the best experts on this subject based on the ideXlab platform.
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involvement of the brain region containing pigment dispersing factor immunoreactive neurons in the photoperiodic response of the bean bug riptortus pedestris
The Journal of Experimental Biology, 2014Co-Authors: Shin G. Goto, Tomoko Ikeno, Hideharu Numata, Sakiko ShigaAbstract:The concept of insect Photoperiodism based on a circadian clock has been supported by many studies demonstrating that the behavioural circadian rhythm and the photoperiodic response are driven by the same circadian clock genes. However, the neuronal mechanism of the circadian clock underlying Photoperiodism is poorly understood. To examine whether circadian rhythm and Photoperiodism share a neuronal mechanism, we focused on the neurons that express neuropeptide pigment-dispersing factor (PDF) in the bean bug, Riptortus pedestris. PDF has been identified as an important regulator of the insect circadian rhythm and is expressed in circadian clock neurons of various insect species. In R. pedestris, PDF immunoreactivity was detected in some clusters of cells and their fibres in the optic lobe and the protocerebrum. cDNA encoding a PDF precursor protein was highly conserved between R. pedestris and many other insects. Differences between day and night were not observed in the immunolabelling intensity in cell bodies of PDF-immunoreactive neurons and pdf mRNA expression levels in the head. Surgical removal of the region containing PDF-immunoreactive cell bodies at the medulla disrupted the photoperiodic regulation of diapause. However, gene suppression of pdf by RNA interference did not affect the photoperiodic response. These results suggest that the region containing PDF-immunoreactive somata is important for the photoperiodic response in R. pedestris, but pdf mRNA expression is probably not required for the response.
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Photoperiodic plasticity in circadian clock neurons in insects
Frontiers in Physiology, 2013Co-Authors: Sakiko ShigaAbstract:Since Bunning’s observation of circadian rhythms and Photoperiodism in the runner bean Phaseolus multiflorus in 1936, many studies have shown that Photoperiodism is based on the circadian clock system. In insects, involvement of circadian clock genes or neurons has been recently shown in the photoperiodic control of developmental arrests, diapause. Based on molecular and neuronal studies in Drosophila melanogaster , photoperiodic changes have been reported for expression patterns of the circadian clock genes, subcellular distribution of clock proteins, fiber distribution, or the number of plausible clock neurons in different species. Photoperiod sets peaks of per or tim mRNA abundance at lights-off in Sarcophaga crassipalpis , Chymomyza costata and Protophormia terraenovae . Abundance of per and Clock mRNA changes by photoperiod in Pyrrhocoris apterus . Subcellular Per distribution in circadian clock neurons changes with photoperiod in P. terraenovae. Although Photoperiodism is not known in Leucophaea maderae, under longer day length, more stomata and longer commissural fibers of circadian clock neurons have been found. These plastic changes in the circadian clock neurons could be an important constituent for photoperiodic clock mechanisms to integrate repetitive photoperiodic information and produce different outputs based on day length.
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different photoreceptor organs are used for Photoperiodism in the larval and adult stages of the carabid beetle leptocarabus kumagaii
The Journal of Experimental Biology, 2009Co-Authors: Yoshinori Shintani, Sakiko Shiga, Hideharu NumataAbstract:SUMMARY The role of the two distinct retinal photoreceptor organs in photoreception for Photoperiodism was examined in the carabid beetle, Leptocarabus kumagaii , by surgical removal. This beetle shows long-day and short-day photoperiodic responses in the larval and adult stages, respectively. Larval diapause in the final instar is induced under short-day conditions whereas pupation occurs without diapause under long-day conditions. Adult reproductive diapause is terminated under short-day conditions but maintained under long-day conditions. The stemmata of the larvae and compound eyes of the adults were removed and the responses of the animals to photoperiod were compared to those of intact beetles. When all the stemmata were removed, larvae pupated without entering diapause under both long-day and short-day conditions, indicating that the larvae lacking stemmata were incapable of photoreception for Photoperiodism. As in other holometabolous insects, the stemmata migrated into the brain during metamorphosis and remained rudimentarily in the optic lobe of the adult brain. However, these stemmata-derived organs were found to be no longer necessary for Photoperiodism, because adults lacking the stemmata-derived organs responded to photoperiod normally. By contrast, removal of the compound eyes in adults resulted in the termination of reproductive diapause under both long-day and short-day conditions, indicating that photoreception for Photoperiodism in the adult stage is performed by the compound eyes. Therefore, the site of photoperiodic photoreception in L. kumagaii appear to change from the stemmata to the compound eyes during metamorphosis.
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roles of per immunoreactive neurons in circadian rhythms and Photoperiodism in the blow fly protophormia terraenovae
The Journal of Experimental Biology, 2009Co-Authors: Sakiko Shiga, Hideharu NumataAbstract:Several hypothetical models suggest that the circadian clock system is involved in the photoperiodic clock mechanisms in insects. However, there is no evidence for this at a neuronal level. In the present study, whether circadian clock neurons were involved in Photoperiodism was examined by surgical ablation of small area in the brain and by immunocytochemical analysis in the blow fly Protophormia terraenovae. Five types of PER-immunoreactive cells, dorsal lateral neurons (LN(d)), large ventral lateral neurons (l-LN(v)), small ventral lateral neurons (s-LN(v)), lateral dorsal neurons (DN(l)) and medial dorsal neurons (DN(m)) were found, corresponding to period-expressing neurons in Drosophila melanogaster. Four l-LN(v)s and four s-LN(v)s were bilaterally double-labelled with antisera against pigment-dispersing factor (PDF) and PER. When the anterior base of the medulla in the optic lobe, where PDF-immunoreactive somata (l-LN(v) and s-LN(v)) are located, was bilaterally ablated, 55% of flies showed arrhythmic or obscure activity patterns under constant darkness. Percentages of flies exhibiting a rhythmic activity pattern decreased along with the number of small PDF-immunoreactive somata (i.e. s-Ln(v)). When regions containing small PDF somata (s-LN(v)) were bilaterally ablated, flies did not discriminate photoperiod, and diapause incidences were 48% under long-day and 55% under short-day conditions. The results suggest that circadian clock neurons, s-LN(v)s, driving behavioural rhythms might also be involved in Photoperiodism, and that circadian behavioural rhythms and Photoperiodism share neural elements in their underlying mechanisms.
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neuroanatomical approaches to the study of insect Photoperiodism
Photochemistry and Photobiology, 2007Co-Authors: Sakiko Shiga, Hideharu NumataAbstract:The anatomical locations of three components of insect Photoperiodism--the photoperiodic photoreceptor, photoperiodic clock and hormonal effector--are summarized and compared between species. Among photoperiodic photoreceptors, either the retinal or extraretinal types or both are operative, and there is no general relationship between phylogeny and photoreceptor type. The photoperiodic clock comprises time measurement and counter systems. Currently, it is generally accepted that circadian oscillators are involved in the photoperiodic clock. Several recent studies have raised the possibility that timeless, a circadian clock gene, plays a role in the photoperiodic clock in flies. The dorsal protocerebrum has been identified as an important region regulating the endocrine system for adult, pupal and embryonic diapause controlled by photoperiod. In the blow fly Protophormia terraenovae, neural connections between circadian clock neurons and indispensable neurons in the pars lateralis for diapause induction in the dorsal protocerebrum have been demonstrated. This neural network may provide the access needed to investigate the neural components of the photoperiodic clock.
Hideharu Numata - One of the best experts on this subject based on the ideXlab platform.
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involvement of the clock gene period in the Photoperiodism of the silkmoth bombyx mori
Zoological Science, 2021Co-Authors: Kento Ikeda, Takaaki Daimon, Kunihiro Shiomi, Hiroko Udaka, Hideharu NumataAbstract:We established a knockout strain of a clock gene, period (per), by using TALEN in a bivoltine strain (Kosetsu) of Bombyx mori (Insecta, Lepidoptera), and examined the effect of per knockout on the circadian rhythm and Photoperiodism. The generated per knockout allele was considered to be null, because a new stop codon was present in the insertion allele. The wild type (Kosetsu) showed clear circadian rhythms in eclosion and hatching, whereas the per knockout strain showed arrhythmic eclosion and hatching under constant darkness. In this strain, moreover, temporal expression changes of clock genes per and timeless were disrupted. The wild type showed a clear long-day response for induction of embryonic diapause: when larvae were reared under long-day and short-day conditions at 25°C, adults produced nondiapause and diapause eggs, respectively. However, the per knockout strain lost the sensitivity to photoperiod and laid nondiapause eggs under both conditions. We conclude that per plays an important role both in circadian rhythms and in Photoperiodism of B. mori, indicating the involvement of the circadian clock consisting of per in the Photoperiodism.
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involvement of the brain region containing pigment dispersing factor immunoreactive neurons in the photoperiodic response of the bean bug riptortus pedestris
The Journal of Experimental Biology, 2014Co-Authors: Shin G. Goto, Tomoko Ikeno, Hideharu Numata, Sakiko ShigaAbstract:The concept of insect Photoperiodism based on a circadian clock has been supported by many studies demonstrating that the behavioural circadian rhythm and the photoperiodic response are driven by the same circadian clock genes. However, the neuronal mechanism of the circadian clock underlying Photoperiodism is poorly understood. To examine whether circadian rhythm and Photoperiodism share a neuronal mechanism, we focused on the neurons that express neuropeptide pigment-dispersing factor (PDF) in the bean bug, Riptortus pedestris. PDF has been identified as an important regulator of the insect circadian rhythm and is expressed in circadian clock neurons of various insect species. In R. pedestris, PDF immunoreactivity was detected in some clusters of cells and their fibres in the optic lobe and the protocerebrum. cDNA encoding a PDF precursor protein was highly conserved between R. pedestris and many other insects. Differences between day and night were not observed in the immunolabelling intensity in cell bodies of PDF-immunoreactive neurons and pdf mRNA expression levels in the head. Surgical removal of the region containing PDF-immunoreactive cell bodies at the medulla disrupted the photoperiodic regulation of diapause. However, gene suppression of pdf by RNA interference did not affect the photoperiodic response. These results suggest that the region containing PDF-immunoreactive somata is important for the photoperiodic response in R. pedestris, but pdf mRNA expression is probably not required for the response.
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photoperiodic diapause under the control of circadian clock genes in an insect
BMC Biology, 2010Co-Authors: Tomoko Ikeno, Hideharu Numata, Shinichi I Tanaka, Shin G. GotoAbstract:Background Most organisms have evolved a circadian clock in order to anticipate daily environmental changes and many of these organisms are also capable of sophisticated measurement of daylength (Photoperiodism) that is used to regulate seasonal events such as diapause, migration and polymorphism. It has been generally accepted that the same elements are involved in both circadian (daily) and seasonal (annual) rhythms because both rely upon daily light-dark cycles. However, as reasonable as this sounds, there remains no conclusive evidence of such a molecular machinery in insects. We have approached this issue by using RNA interference (RNAi) in Riptortus pedestris.
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different photoreceptor organs are used for Photoperiodism in the larval and adult stages of the carabid beetle leptocarabus kumagaii
The Journal of Experimental Biology, 2009Co-Authors: Yoshinori Shintani, Sakiko Shiga, Hideharu NumataAbstract:SUMMARY The role of the two distinct retinal photoreceptor organs in photoreception for Photoperiodism was examined in the carabid beetle, Leptocarabus kumagaii , by surgical removal. This beetle shows long-day and short-day photoperiodic responses in the larval and adult stages, respectively. Larval diapause in the final instar is induced under short-day conditions whereas pupation occurs without diapause under long-day conditions. Adult reproductive diapause is terminated under short-day conditions but maintained under long-day conditions. The stemmata of the larvae and compound eyes of the adults were removed and the responses of the animals to photoperiod were compared to those of intact beetles. When all the stemmata were removed, larvae pupated without entering diapause under both long-day and short-day conditions, indicating that the larvae lacking stemmata were incapable of photoreception for Photoperiodism. As in other holometabolous insects, the stemmata migrated into the brain during metamorphosis and remained rudimentarily in the optic lobe of the adult brain. However, these stemmata-derived organs were found to be no longer necessary for Photoperiodism, because adults lacking the stemmata-derived organs responded to photoperiod normally. By contrast, removal of the compound eyes in adults resulted in the termination of reproductive diapause under both long-day and short-day conditions, indicating that photoreception for Photoperiodism in the adult stage is performed by the compound eyes. Therefore, the site of photoperiodic photoreception in L. kumagaii appear to change from the stemmata to the compound eyes during metamorphosis.
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roles of per immunoreactive neurons in circadian rhythms and Photoperiodism in the blow fly protophormia terraenovae
The Journal of Experimental Biology, 2009Co-Authors: Sakiko Shiga, Hideharu NumataAbstract:Several hypothetical models suggest that the circadian clock system is involved in the photoperiodic clock mechanisms in insects. However, there is no evidence for this at a neuronal level. In the present study, whether circadian clock neurons were involved in Photoperiodism was examined by surgical ablation of small area in the brain and by immunocytochemical analysis in the blow fly Protophormia terraenovae. Five types of PER-immunoreactive cells, dorsal lateral neurons (LN(d)), large ventral lateral neurons (l-LN(v)), small ventral lateral neurons (s-LN(v)), lateral dorsal neurons (DN(l)) and medial dorsal neurons (DN(m)) were found, corresponding to period-expressing neurons in Drosophila melanogaster. Four l-LN(v)s and four s-LN(v)s were bilaterally double-labelled with antisera against pigment-dispersing factor (PDF) and PER. When the anterior base of the medulla in the optic lobe, where PDF-immunoreactive somata (l-LN(v) and s-LN(v)) are located, was bilaterally ablated, 55% of flies showed arrhythmic or obscure activity patterns under constant darkness. Percentages of flies exhibiting a rhythmic activity pattern decreased along with the number of small PDF-immunoreactive somata (i.e. s-Ln(v)). When regions containing small PDF somata (s-LN(v)) were bilaterally ablated, flies did not discriminate photoperiod, and diapause incidences were 48% under long-day and 55% under short-day conditions. The results suggest that circadian clock neurons, s-LN(v)s, driving behavioural rhythms might also be involved in Photoperiodism, and that circadian behavioural rhythms and Photoperiodism share neural elements in their underlying mechanisms.
William E Bradshaw - One of the best experts on this subject based on the ideXlab platform.
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natural variation and genetics of Photoperiodism in wyeomyia smithii
Advances in Genetics, 2017Co-Authors: William E Bradshaw, Christina M HolzapfelAbstract:Seasonal change in the temperate and polar regions of Earth determines how the world looks around us and, in fact, how we live our day-to-day lives. For biological organisms, seasonal change typically involves complex physiological and metabolic reorganization, the majority of which is regulated by Photoperiodism. Photoperiodism is the ability of animals and plants to use day length or night length, resulting in life-historical transformations, including seasonal development, migration, reproduction, and dormancy. Seasonal timing determines not only survival and reproductive success but also the structure and organization of complex communities and, ultimately, the biomes of Earth. Herein, a small mosquito, Wyeomyia smithii, that lives only in the water-filled leaves of a carnivorous plant over a wide geographic range, is used to explore the genetic and evolutionary basis of Photoperiodism. Photoperiodism in W. smithii is considered in the context of its historical biogeography in nature to examine the startling finding that recent rapid climate change can drive genetic change in plants and animals at break-neck speed, and to challenge the ponderous 80+ year search for connections between daily and seasonal time-keeping mechanisms. Finally, a model is proposed that reconciles the seemingly disparate 24-h daily clock driven by the invariant rotation of Earth about its axis with the evolutionarily flexible seasonal timer orchestrated by variable seasonality driven by the rotation of Earth about the Sun.
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complications of complexity integrating environmental genetic and hormonal control of insect diapause
Trends in Genetics, 2009Co-Authors: Kevin J Emerson, William E Bradshaw, Christina M HolzapfelAbstract:Understanding gene interaction and pleiotropy are long-standing goals of developmental and evolutionary biology. We examine the genetic control of diapause in insects and show how the failure to recognize the difference between modular and gene pleiotropy has confounded our understanding of the genetic basis of this important phenotype. This has led to complications in understanding the role of the circadian clock in the control of diapause in Drosophila and other insects. We emphasize three successive modules – each containing functionally related genes – that lead to diapause: Photoperiodism, hormonal events and diapause itself. Understanding the genetic basis for environmental control of diapause has wider implications for evolutionary response to rapid climate change and for the opportunity to observe evolutionary change in contemporary time.
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Evolution of photoperiodic time measurement is independent of the circadian clock in the pitcher-plant mosquito, Wyeomyia smithii
Journal of Comparative Physiology A, 2009Co-Authors: K J Emerson, William E Bradshaw, Sabrina J. Dake, Christina M HolzapfelAbstract:For over 70 years, researchers have debated whether the ability to use day length as a cue for the timing of seasonal events (Photoperiodism) is related to the endogenous circadian clock that regulates the timing of daily events. Models of Photoperiodism include two components: (1) a photoperiodic timer that measures the length of the day, and (2) a photoperiodic counter that elicits the downstream photoperiodic response after a threshold number of days has been counted. Herein, we show that there is no geographical pattern of genetic association between the expression of the circadian clock and the photoperiodic timer or counter. We conclude that the photoperiodic timer and counter have evolved independently of the circadian clock in the pitcher-plant mosquito Wyeomyia smithii and hence, the evolutionary modification of Photoperiodism throughout the range of W. smithii has not been causally mediated by a corresponding evolution of the circadian clock.
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quantitative trait loci associated with photoperiodic response and stage of diapause in the pitcher plant mosquito wyeomyia smithii
Genetics, 2007Co-Authors: Derrick Mathias, William E Bradshaw, Lucien Jacky, Christina M HolzapfelAbstract:A wide variety of temperate animals rely on length of day (Photoperiodism) to anticipate and prepare for changing seasons by regulating the timing of development, reproduction, dormancy, and migration. Although the molecular basis of circadian rhythms regulating daily activities is well defined, the molecular basis for the photoperiodic regulation of seasonal activities is largely unknown. We use geographic variation in the photoperiodic control of diapause in the pitcher-plant mosquito Wyeomyia smithii to create the first QTL map of Photoperiodism in any animal. For critical photoperiod (CPP), we detect QTL that are unique, a QTL that is sex linked, QTL that overlap with QTL for stage of diapause (SOD), and a QTL that interacts epistatically with the circadian rhythm gene, timeless. Results presented here confirm earlier studies concluding that CPP is under directional selection over the climatic gradient of North America and that the evolution of CPP is genetically correlated with SOD. Despite epistasis between timeless and a QTL for CPP, timeless is not located within any detectable QTL, indicating that it plays an ancillary role in the evolution of Photoperiodism in W. smithii. Finally, we highlight one region of the genome that includes loci contributing to CPP, SOD, and hormonal regulation of development.
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Evolution of Animal Photoperiodism
Annual Review of Ecology Evolution and Systematics, 2007Co-Authors: William E Bradshaw, Christina M HolzapfelAbstract:AbstractPhotoperiodism is the ability of organisms to assess and use the day length as an anticipatory cue to time seasonal events in their life histories. Photoperiodism is especially important in initiating physiological and developmental processes that are typically irrevocable and that culminate at a future time or at a distant place; the further away in space or time, the more likely a seasonal event is initiated by photoperiod. The pervasiveness of Photoperiodism across broad taxa, from rotifers to rodents, and the predictable changes of photoperiodic response with geography identify it as a central component of fitness in temperate and polar seasonal environments. Consequently, the role of day length cannot be disregarded when evaluating the mechanisms underlying life-historical events, range expansions, invasions of novel species, and response to climate change among animals in the temperate and polar regions of the world.
Makio Takeda - One of the best experts on this subject based on the ideXlab platform.
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N-acetyltransferase (nat) Is a Critical Conjunct of Photoperiodism between the Circadian System and Endocrine Axis in Antheraea pernyi
2016Co-Authors: Ahmed A. M. Mohamed, Qiushi Wang, Naoyuki Ichihara, Makio TakedaAbstract:Since its discovery in 1923, the biology of Photoperiodism remains a mystery in many ways. We sought the link connecting the circadian system to an endocrine switch, using Antheraea pernyi. PER-, CLK- and CYC-ir were co-expressed in two pairs of dorsolateral neurons of the protocerebrum, suggesting that these are the circadian neurons that also express melatonin-, NAT- and HIOMT-ir. The results suggest that a melatonin pathway is present in the circadian neurons. Melatonin receptor (MT2 or MEL-1B-R)-ir in PTTH-ir neurons juxtaposing clock neurons suggests that melatonin gates PTTH release. RIA showed a melatonin rhythm with a peak four hours after lights off in adult brain both under LD16:8 (LD) and LD12:12 (SD), and both the peak and the baseline levels were higher under LD than SD, suggesting a photoperiodic influence. When pupae in diapause were exposed to 10 cycles of LD, or stored at 4uC for 4 months under constant darkness, an increase of NAT activity was observed when PTTH released ecdysone. DNA sequence upstream of nat contained E-boxes to which CYC/CLK could bind, and nat transcription was turned off by clk or cyc dsRNA. dsRNANAT caused dysfunction of Photoperiodism. dsRNAPER upregulated nat transcription as anticipated, based on findings in the Drosophila melanogaster circadian system. Transcription of nat, cyc and clk peaked at ZT12. RIA showed that dsRNANAT decreased melatonin while dsRNAPER increased melatonin. Thus nat, a clock controlled gene, is the critical link between the circadian clock and endocrine switch. MT-binding may release PTTH, resulting in termination of diapause. This study thus examined all of the basic functional unit
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structures and functions of insect arylalkylamine n acetyltransferase iaanat a key enzyme for physiological and behavioral switch in arthropods
Frontiers in Physiology, 2015Co-Authors: Susumu Hiragaki, Ahmed A. M. Mohamed, Takeshi Suzuki, Makio TakedaAbstract:The evolution of N-acetyltransfeases (NATs) seems complex. Vertebrate arylalkylamine N-acetyltransferase (aaNAT) has been extensively studied since it leads to the synthesis of melatonin, a multifunctional neurohormone prevalent in photoreceptor cells, and is known as a chemical token of the night. Melatonin also serves as a scavenger for reactive oxygen species. This is also true with invertebrates. NAT therefore has distinct functional implications in circadian function, as timezymes (aaNAT), and also xenobiotic reactions (arylamine NAT or simply NAT). NATs belong to a broader enzyme group, the GCN5-related N-acetyltransferase superfamily. Due to low sequence homology and a seemingly fast rate of structural differentiation, the nomenclature for NATs can be confusing. The advent of bioinformatics, however, has helped to classify this group of enzymes; vertebrates have two distinct subgroups, the timezyme type and the xenobiotic type, which has a wider substrate range including imidazolamine, pharmacological drugs, environmental toxicants and even histone. Insect aaNAT (iaaNAT) form their own clade in the phylogeny, distinct from vertebrate aaNATs. Arthropods are unique, since the phylum has exoskeleton in which quinones derived from N-acetylated monoamines function in coupling chitin and arthropodins. Monoamine oxidase (MAO) activity is limited in insects, but NAT-mediated degradation prevails. However, unexpectedly iaaNAT occurs not only among arthropods but also among basal deuterostomia, and is therefore more apomorphic. Our analyses illustrate that iaaNATs has unique physiological roles but at the same time it plays a role in a timezyme function, at least in Photoperiodism. Photoperiodism has been considered as a function of circadian system but the detailed molecular mechanism is not well understood. We propose a molecular hypothesis for Photoperiodism in Antheraea pernyi based on the transcription regulation of NAT interlocked by the circadian system. Therefore, the enzyme plays both unique and universal roles in insects. The unique role of iaaNATs in physiological regulation urges the targeting of this system for integrated pest management (IPM). We indeed showed a successful example of chemical compound screening with reconstituted enzyme and further attempts seem promising.
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n acetyltransferase nat is a critical conjunct of Photoperiodism between the circadian system and endocrine axis in antheraea pernyi
PLOS ONE, 2014Co-Authors: Ahmed A. M. Mohamed, Qiushi Wang, Naoyuki Ichihara, Jadwiga Bembenek, Susumu Hiragaki, Takeshi Suzuki, Makio TakedaAbstract:Since its discovery in 1923, the biology of Photoperiodism remains a mystery in many ways. We sought the link connecting the circadian system to an endocrine switch, using Antheraea pernyi. PER-, CLK- and CYC-ir were co-expressed in two pairs of dorsolateral neurons of the protocerebrum, suggesting that these are the circadian neurons that also express melatonin-, NAT- and HIOMT-ir. The results suggest that a melatonin pathway is present in the circadian neurons. Melatonin receptor (MT2 or MEL-1B-R)-ir in PTTH-ir neurons juxtaposing clock neurons suggests that melatonin gates PTTH release. RIA showed a melatonin rhythm with a peak four hours after lights off in adult brain both under LD16∶8 (LD) and LD12∶12 (SD), and both the peak and the baseline levels were higher under LD than SD, suggesting a photoperiodic influence. When pupae in diapause were exposed to 10 cycles of LD, or stored at 4°C for 4 months under constant darkness, an increase of NAT activity was observed when PTTH released ecdysone. DNA sequence upstream of nat contained E-boxes to which CYC/CLK could bind, and nat transcription was turned off by clk or cyc dsRNA. dsRNANAT caused dysfunction of Photoperiodism. dsRNAPER upregulated nat transcription as anticipated, based on findings in the Drosophila melanogaster circadian system. Transcription of nat, cyc and clk peaked at ZT12. RIA showed that dsRNANAT decreased melatonin while dsRNAPER increased melatonin. Thus nat, a clock controlled gene, is the critical link between the circadian clock and endocrine switch. MT-binding may release PTTH, resulting in termination of diapause. This study thus examined all of the basic functional units from the clock: a photoperiodic counter as an accumulator of mRNANAT, to endocrine switch for Photoperiodism in A. pernyi showing this system is self-complete without additional device especially for Photoperiodism.