The Experts below are selected from a list of 16071 Experts worldwide ranked by ideXlab platform

Guojun Chen - One of the best experts on this subject based on the ideXlab platform.

  • the microrna mir 124 suppresses Seizure activity and regulates creb1 activity
    Expert Reviews in Molecular Medicine, 2016
    Co-Authors: Wei Wang, Xuefeng Wang, Lang Chen, Yujiao Zhang, Jing Liu, Guohui Jiang, Xiaogang Zhang, Kewei Wang, Jinghui Wang, Guojun Chen
    Abstract:

    miR-124, a brain-specific microRNA, was originally considered as a key regulator in neuronal differentiation and the development of the nervous system. Here we showed that miR-124 expression was suppressed in patients with epilepsy and rats after drug induced-Seizures. Intrahippocampal administration of a miR-124 duplex led to alleviated Seizure severity and prolonged onset latency in two rat models (pentylenetetrazole- and Pilocarpine-Induced Seizures), while miR-124 inhibitor led to shortened onset latency in Pilocarpine-Induced Seizure rat models. Moreover, the result of local field potentials (LFPs) records further demonstrated miR-124 may have anti-epilepsy function. Inhibition of neuronal firing by miR-124 was associated with the suppression of mEPSC, AMPAR- and NMDAR-mediated currents, which were accompanied by decreased surface expression of NMDAR. In addition, miR-124 injection resulted in decreased activity and expression of cAMP-response element-binding protein1 (CREB1). a key regulator in epileptogenesis. A dual-luciferase reporter assay was used to confirm that miR-124 targeted directly the 3'UTR of CREB1 gene and repressed the CREB1 expression in HEK293T cells. Immunoprecipitation studies confirmed that the CREB1 antibody effectively precipitated CREB1 and NMDAR1 but not GLUR1 from rat brain hippocampus. These results revealed a previously unknown function of miR-124 in neuronal excitability and provided a new insight into molecular mechanisms underlying epilepsy.

Wang Kewei - One of the best experts on this subject based on the ideXlab platform.

  • The microRNA miR-124 suppresses Seizure activity and regulates CREB1 activity
    EXPERT REVIEWS IN MOLECULAR MEDICINE, 2016
    Co-Authors: Wang Wei, Wang Xuefeng, Chen Lang, Zhang Yujiao, Xu Zucai, Liu Jing, Jiang Guohui, Li Jie, Zhang Xiaogang, Wang Kewei
    Abstract:

    miR-124, a brain-specific microRNA, was originally considered as a key regulator in neuronal differentiation and the development of the nervous system. Here we showed that miR-124 expression was suppressed in patients with epilepsy and rats after drug induced-Seizures. Intrahippocampal administration of a miR-124 duplex led to alleviated Seizure severity and prolonged onset latency in two rat models (pentylenetetrazole- and Pilocarpine-Induced Seizures), while miR-124 inhibitor led to shortened onset latency in Pilocarpine-Induced Seizure rat models. Moreover, the result of local field potentials (LFPs) records further demonstrated miR-124 may have anti-epilepsy function. Inhibition of neuronal firing by miR-124 was associated with the suppression of mEPSC, AMPAR- and NMDAR-mediated currents, which were accompanied by decreased surface expression of NMDAR. In addition, miR-124 injection resulted in decreased activity and expression of cAMP-response element-binding protein1 (CREB1). a key regulator in epileptogenesis. A dual-luciferase reporter assay was used to confirm that miR-124 targeted directly the 3'UTR of CREB1 gene and repressed the CREB1 expression in HEK293T cells. Immunoprecipitation studies confirmed that the CREB1 antibody effectively precipitated CREB1 and NMDAR1 but not GLUR1 from rat brain hippocampus. These results revealed a previously unknown function of miR-124 in neuronal excitability and provided a new insight into molecular mechanisms underlying epilepsy.National Natural Science Foundation of China [81271445, 81501122]; National Clinical Key Specialty Construction Foundation of China; National Institutes of Health of ChinaSCI(E)PubMedREVIEWjgire@163.come41

Wei Wang - One of the best experts on this subject based on the ideXlab platform.

  • the microrna mir 124 suppresses Seizure activity and regulates creb1 activity
    Expert Reviews in Molecular Medicine, 2016
    Co-Authors: Wei Wang, Xuefeng Wang, Lang Chen, Yujiao Zhang, Jing Liu, Guohui Jiang, Xiaogang Zhang, Kewei Wang, Jinghui Wang, Guojun Chen
    Abstract:

    miR-124, a brain-specific microRNA, was originally considered as a key regulator in neuronal differentiation and the development of the nervous system. Here we showed that miR-124 expression was suppressed in patients with epilepsy and rats after drug induced-Seizures. Intrahippocampal administration of a miR-124 duplex led to alleviated Seizure severity and prolonged onset latency in two rat models (pentylenetetrazole- and Pilocarpine-Induced Seizures), while miR-124 inhibitor led to shortened onset latency in Pilocarpine-Induced Seizure rat models. Moreover, the result of local field potentials (LFPs) records further demonstrated miR-124 may have anti-epilepsy function. Inhibition of neuronal firing by miR-124 was associated with the suppression of mEPSC, AMPAR- and NMDAR-mediated currents, which were accompanied by decreased surface expression of NMDAR. In addition, miR-124 injection resulted in decreased activity and expression of cAMP-response element-binding protein1 (CREB1). a key regulator in epileptogenesis. A dual-luciferase reporter assay was used to confirm that miR-124 targeted directly the 3'UTR of CREB1 gene and repressed the CREB1 expression in HEK293T cells. Immunoprecipitation studies confirmed that the CREB1 antibody effectively precipitated CREB1 and NMDAR1 but not GLUR1 from rat brain hippocampus. These results revealed a previously unknown function of miR-124 in neuronal excitability and provided a new insight into molecular mechanisms underlying epilepsy.

Wang Wei - One of the best experts on this subject based on the ideXlab platform.

  • The microRNA miR-124 suppresses Seizure activity and regulates CREB1 activity
    EXPERT REVIEWS IN MOLECULAR MEDICINE, 2016
    Co-Authors: Wang Wei, Wang Xuefeng, Chen Lang, Zhang Yujiao, Xu Zucai, Liu Jing, Jiang Guohui, Li Jie, Zhang Xiaogang, Wang Kewei
    Abstract:

    miR-124, a brain-specific microRNA, was originally considered as a key regulator in neuronal differentiation and the development of the nervous system. Here we showed that miR-124 expression was suppressed in patients with epilepsy and rats after drug induced-Seizures. Intrahippocampal administration of a miR-124 duplex led to alleviated Seizure severity and prolonged onset latency in two rat models (pentylenetetrazole- and Pilocarpine-Induced Seizures), while miR-124 inhibitor led to shortened onset latency in Pilocarpine-Induced Seizure rat models. Moreover, the result of local field potentials (LFPs) records further demonstrated miR-124 may have anti-epilepsy function. Inhibition of neuronal firing by miR-124 was associated with the suppression of mEPSC, AMPAR- and NMDAR-mediated currents, which were accompanied by decreased surface expression of NMDAR. In addition, miR-124 injection resulted in decreased activity and expression of cAMP-response element-binding protein1 (CREB1). a key regulator in epileptogenesis. A dual-luciferase reporter assay was used to confirm that miR-124 targeted directly the 3'UTR of CREB1 gene and repressed the CREB1 expression in HEK293T cells. Immunoprecipitation studies confirmed that the CREB1 antibody effectively precipitated CREB1 and NMDAR1 but not GLUR1 from rat brain hippocampus. These results revealed a previously unknown function of miR-124 in neuronal excitability and provided a new insight into molecular mechanisms underlying epilepsy.National Natural Science Foundation of China [81271445, 81501122]; National Clinical Key Specialty Construction Foundation of China; National Institutes of Health of ChinaSCI(E)PubMedREVIEWjgire@163.come41

Guohui Jiang - One of the best experts on this subject based on the ideXlab platform.

  • the microrna mir 124 suppresses Seizure activity and regulates creb1 activity
    Expert Reviews in Molecular Medicine, 2016
    Co-Authors: Wei Wang, Xuefeng Wang, Lang Chen, Yujiao Zhang, Jing Liu, Guohui Jiang, Xiaogang Zhang, Kewei Wang, Jinghui Wang, Guojun Chen
    Abstract:

    miR-124, a brain-specific microRNA, was originally considered as a key regulator in neuronal differentiation and the development of the nervous system. Here we showed that miR-124 expression was suppressed in patients with epilepsy and rats after drug induced-Seizures. Intrahippocampal administration of a miR-124 duplex led to alleviated Seizure severity and prolonged onset latency in two rat models (pentylenetetrazole- and Pilocarpine-Induced Seizures), while miR-124 inhibitor led to shortened onset latency in Pilocarpine-Induced Seizure rat models. Moreover, the result of local field potentials (LFPs) records further demonstrated miR-124 may have anti-epilepsy function. Inhibition of neuronal firing by miR-124 was associated with the suppression of mEPSC, AMPAR- and NMDAR-mediated currents, which were accompanied by decreased surface expression of NMDAR. In addition, miR-124 injection resulted in decreased activity and expression of cAMP-response element-binding protein1 (CREB1). a key regulator in epileptogenesis. A dual-luciferase reporter assay was used to confirm that miR-124 targeted directly the 3'UTR of CREB1 gene and repressed the CREB1 expression in HEK293T cells. Immunoprecipitation studies confirmed that the CREB1 antibody effectively precipitated CREB1 and NMDAR1 but not GLUR1 from rat brain hippocampus. These results revealed a previously unknown function of miR-124 in neuronal excitability and provided a new insight into molecular mechanisms underlying epilepsy.