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P D Chaudhari - One of the best experts on this subject based on the ideXlab platform.

  • Production of high titre antibody response against Russell's viper venom in mice immunized with ethanolic extract of fruits of Piper Longum L. (Piperaceae) and Piperine.
    Phytomedicine : international journal of phytotherapy and phytopharmacology, 2013
    Co-Authors: P A Shenoy, S S Nipate, J M Sonpetkar, N C Salvi, A B Waghmare, P D Chaudhari
    Abstract:

    Abstract Piper Longum L. fruits have been traditionally used against snakebites in north-eastern and southern region of India. The aim of the study was to assess the production of antibody response against Russell's viper venom in mice after prophylactic immunization with ethanolic extract of fruits of Piper Longum L. and Piperine. The mice sera were tested for the presence of antibodies against Russell's viper venom by in vitro lethality neutralization assay and in vivo lethality neutralization assay. Polyvalent anti-snake venom serum (antivenom) manufactured by Haffkine Bio-Pharmaceutical Corporation Ltd. was used as standard. Further confirmation of presence of antibodies against the venom in sera of mice immunized with PLE and Piperine was done using indirect enzyme-linked immunosorbent assay (ELISA) and double immunodiffusion test. Treatment with PLE-treated mice serum and Piperine-treated mice serum was found to inhibit the lethal action of venom both in the in vitro lethality neutralization assay and in vivo lethality neutralization assay. ELISA testing indicated that there were significantly high ( p Piper Longum and Piperine produced a high titre antibody response against Russell's viper venom in mice. The antibodies against PLE and Piperine could be useful in antivenom therapy of Russell's viper bites. PLE and Piperine may also have a potential interest in view of the development of antivenom formulations used as antidote against snake bites.

  • anti snake venom activities of ethanolic extract of fruits of Piper Longum l Piperaceae against russell s viper venom characterization of Piperine as active principle
    Journal of Ethnopharmacology, 2013
    Co-Authors: P A Shenoy, S S Nipate, J M Sonpetkar, N C Salvi, A B Waghmare, P D Chaudhari
    Abstract:

    ETHNOPHARMACOLOGICAL RELEVANCE: Piper Longum L. fruits have been traditionally used against snakebites in north-eastern and southern region of India. AIM OF THE STUDY: To examine the ability of ethanolic extract of fruits of Piper Longum L., Piperaceae (PLE) and Piperine, one of the main active principles of Piper Longum, to inhibit the Russell's viper (Doboia russelii, Viperidae) snake venom activities. MATERIALS AND METHODS: Anti-snake venom activities of ethanolic extract of fruits of Piper Longum L. (Piperaceae) and Piperine against Russell's viper venom was studied in embryonated fertile chicken eggs, mice and rats by using various models as follows: inhibition of venom lethal action, inhibition of venom haemorrhagic action (in vitro), inhibition of venom haemorrhagic action (in vivo), inhibition of venom necrotizing action, inhibition of venom defibrinogenating action, inhibition of venom induced paw edema, inhibition of venom induced mast cell degranulation, creatine kinase assay and assay for catalase activity. RESULTS: PLE was found to inhibit the venom induced haemorrhage in embryonated fertile chicken eggs. Administration of PLE and Piperine significantly (p<0.01) inhibited venom induced lethality, haemorrhage, necrosis, defibrinogenation and inflammatory paw edema in mice in a dose dependent manner. PLE and Piperine also significantly (p<0.01) reduced venom induced mast cell degranulation in rats. Venom induced decrease in catalase enzyme levels in mice kidney tissue and increase in creatine kinase enzyme levels in mice serum were significantly (p<0.01) reversed by administration of both PLE and Piperine. CONCLUSIONS: PLE possesses good anti-snake venom properties and Piperine is one of the compounds responsible for the effective venom neutralizing ability of the plant.

  • Anti-snake venom activities of ethanolic extract of fruits of Piper Longum L. (Piperaceae) against Russell's viper venom: characterization of Piperine as active principle.
    Journal of ethnopharmacology, 2013
    Co-Authors: P A Shenoy, S S Nipate, J M Sonpetkar, N C Salvi, A B Waghmare, P D Chaudhari
    Abstract:

    Abstract Ethnopharmacological relevance Piper Longum L. fruits have been traditionally used against snakebites in north-eastern and southern region of India. Aim of the study To examine the ability of ethanolic extract of fruits of Piper Longum L., Piperaceae (PLE) and Piperine, one of the main active principles of Piper Longum, to inhibit the Russell's viper (Doboia russelii, Viperidae) snake venom activities. Materials and methods Anti-snake venom activities of ethanolic extract of fruits of Piper Longum L. (Piperaceae) and Piperine against Russell's viper venom was studied in embryonated fertile chicken eggs, mice and rats by using various models as follows: inhibition of venom lethal action, inhibition of venom haemorrhagic action (in vitro), inhibition of venom haemorrhagic action (in vivo), inhibition of venom necrotizing action, inhibition of venom defibrinogenating action, inhibition of venom induced paw edema, inhibition of venom induced mast cell degranulation, creatine kinase assay and assay for catalase activity. Results PLE was found to inhibit the venom induced haemorrhage in embryonated fertile chicken eggs. Administration of PLE and Piperine significantly (p Conclusions PLE possesses good anti-snake venom properties and Piperine is one of the compounds responsible for the effective venom neutralizing ability of the plant.

Xinmiao Liang - One of the best experts on this subject based on the ideXlab platform.

  • Purification of amide alkaloids from Piper Longum L. using preparative two-dimensional normal-phase liquid chromatography × reversed-phase liquid chromatography
    The Analyst, 2013
    Co-Authors: Wenya Zhu, Yu Jin, Xinmiao Liang
    Abstract:

    A comprehensive off-line two-dimensional liquid chromatography (2D-LC) method coupling normal phase liquid chromatography (NPLC) and reversed phase liquid chromatography (RPLC) was developed for separation and purification of amide alkaloids from Piper Longum L. In the first dimension, the crude alkaloid fractions were separated in NPLC mode and 20 fractions were collected. Then fractions 5–20 were selected for further purification in RPLC mode in the second dimension. The purities of RPLC fractions with similar structures were all identified accurately by ultra performance liquid chromatography (UPLC). In total, 28 compounds with high purity were obtained and their structures were comprehensively characterized by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) spectroscopy. The results demonstrate that this 2D NPLC × RPLC method with good orthogonality (58.3%) was effective for the preparative separation and purification of amide alkaloids from Piper Longum L.

  • purification of amide alkaloids from Piper Longum l using preparative two dimensional normal phase liquid chromatography reversed phase liquid chromatography
    Analyst, 2013
    Co-Authors: Wenya Zhu, Yu Jin, Xinmiao Liang
    Abstract:

    A comprehensive off-line two-dimensional liquid chromatography (2D-LC) method coupling normal phase liquid chromatography (NPLC) and reversed phase liquid chromatography (RPLC) was developed for separation and purification of amide alkaloids from Piper Longum L. In the first dimension, the crude alkaloid fractions were separated in NPLC mode and 20 fractions were collected. Then fractions 5–20 were selected for further purification in RPLC mode in the second dimension. The purities of RPLC fractions with similar structures were all identified accurately by ultra performance liquid chromatography (UPLC). In total, 28 compounds with high purity were obtained and their structures were comprehensively characterized by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) spectroscopy. The results demonstrate that this 2D NPLC × RPLC method with good orthogonality (58.3%) was effective for the preparative separation and purification of amide alkaloids from Piper Longum L.

P A Shenoy - One of the best experts on this subject based on the ideXlab platform.

  • Production of high titre antibody response against Russell's viper venom in mice immunized with ethanolic extract of fruits of Piper Longum L. (Piperaceae) and Piperine.
    Phytomedicine : international journal of phytotherapy and phytopharmacology, 2013
    Co-Authors: P A Shenoy, S S Nipate, J M Sonpetkar, N C Salvi, A B Waghmare, P D Chaudhari
    Abstract:

    Abstract Piper Longum L. fruits have been traditionally used against snakebites in north-eastern and southern region of India. The aim of the study was to assess the production of antibody response against Russell's viper venom in mice after prophylactic immunization with ethanolic extract of fruits of Piper Longum L. and Piperine. The mice sera were tested for the presence of antibodies against Russell's viper venom by in vitro lethality neutralization assay and in vivo lethality neutralization assay. Polyvalent anti-snake venom serum (antivenom) manufactured by Haffkine Bio-Pharmaceutical Corporation Ltd. was used as standard. Further confirmation of presence of antibodies against the venom in sera of mice immunized with PLE and Piperine was done using indirect enzyme-linked immunosorbent assay (ELISA) and double immunodiffusion test. Treatment with PLE-treated mice serum and Piperine-treated mice serum was found to inhibit the lethal action of venom both in the in vitro lethality neutralization assay and in vivo lethality neutralization assay. ELISA testing indicated that there were significantly high ( p Piper Longum and Piperine produced a high titre antibody response against Russell's viper venom in mice. The antibodies against PLE and Piperine could be useful in antivenom therapy of Russell's viper bites. PLE and Piperine may also have a potential interest in view of the development of antivenom formulations used as antidote against snake bites.

  • anti snake venom activities of ethanolic extract of fruits of Piper Longum l Piperaceae against russell s viper venom characterization of Piperine as active principle
    Journal of Ethnopharmacology, 2013
    Co-Authors: P A Shenoy, S S Nipate, J M Sonpetkar, N C Salvi, A B Waghmare, P D Chaudhari
    Abstract:

    ETHNOPHARMACOLOGICAL RELEVANCE: Piper Longum L. fruits have been traditionally used against snakebites in north-eastern and southern region of India. AIM OF THE STUDY: To examine the ability of ethanolic extract of fruits of Piper Longum L., Piperaceae (PLE) and Piperine, one of the main active principles of Piper Longum, to inhibit the Russell's viper (Doboia russelii, Viperidae) snake venom activities. MATERIALS AND METHODS: Anti-snake venom activities of ethanolic extract of fruits of Piper Longum L. (Piperaceae) and Piperine against Russell's viper venom was studied in embryonated fertile chicken eggs, mice and rats by using various models as follows: inhibition of venom lethal action, inhibition of venom haemorrhagic action (in vitro), inhibition of venom haemorrhagic action (in vivo), inhibition of venom necrotizing action, inhibition of venom defibrinogenating action, inhibition of venom induced paw edema, inhibition of venom induced mast cell degranulation, creatine kinase assay and assay for catalase activity. RESULTS: PLE was found to inhibit the venom induced haemorrhage in embryonated fertile chicken eggs. Administration of PLE and Piperine significantly (p<0.01) inhibited venom induced lethality, haemorrhage, necrosis, defibrinogenation and inflammatory paw edema in mice in a dose dependent manner. PLE and Piperine also significantly (p<0.01) reduced venom induced mast cell degranulation in rats. Venom induced decrease in catalase enzyme levels in mice kidney tissue and increase in creatine kinase enzyme levels in mice serum were significantly (p<0.01) reversed by administration of both PLE and Piperine. CONCLUSIONS: PLE possesses good anti-snake venom properties and Piperine is one of the compounds responsible for the effective venom neutralizing ability of the plant.

  • Anti-snake venom activities of ethanolic extract of fruits of Piper Longum L. (Piperaceae) against Russell's viper venom: characterization of Piperine as active principle.
    Journal of ethnopharmacology, 2013
    Co-Authors: P A Shenoy, S S Nipate, J M Sonpetkar, N C Salvi, A B Waghmare, P D Chaudhari
    Abstract:

    Abstract Ethnopharmacological relevance Piper Longum L. fruits have been traditionally used against snakebites in north-eastern and southern region of India. Aim of the study To examine the ability of ethanolic extract of fruits of Piper Longum L., Piperaceae (PLE) and Piperine, one of the main active principles of Piper Longum, to inhibit the Russell's viper (Doboia russelii, Viperidae) snake venom activities. Materials and methods Anti-snake venom activities of ethanolic extract of fruits of Piper Longum L. (Piperaceae) and Piperine against Russell's viper venom was studied in embryonated fertile chicken eggs, mice and rats by using various models as follows: inhibition of venom lethal action, inhibition of venom haemorrhagic action (in vitro), inhibition of venom haemorrhagic action (in vivo), inhibition of venom necrotizing action, inhibition of venom defibrinogenating action, inhibition of venom induced paw edema, inhibition of venom induced mast cell degranulation, creatine kinase assay and assay for catalase activity. Results PLE was found to inhibit the venom induced haemorrhage in embryonated fertile chicken eggs. Administration of PLE and Piperine significantly (p Conclusions PLE possesses good anti-snake venom properties and Piperine is one of the compounds responsible for the effective venom neutralizing ability of the plant.

Wenya Zhu - One of the best experts on this subject based on the ideXlab platform.

  • Purification of amide alkaloids from Piper Longum L. using preparative two-dimensional normal-phase liquid chromatography × reversed-phase liquid chromatography
    The Analyst, 2013
    Co-Authors: Wenya Zhu, Yu Jin, Xinmiao Liang
    Abstract:

    A comprehensive off-line two-dimensional liquid chromatography (2D-LC) method coupling normal phase liquid chromatography (NPLC) and reversed phase liquid chromatography (RPLC) was developed for separation and purification of amide alkaloids from Piper Longum L. In the first dimension, the crude alkaloid fractions were separated in NPLC mode and 20 fractions were collected. Then fractions 5–20 were selected for further purification in RPLC mode in the second dimension. The purities of RPLC fractions with similar structures were all identified accurately by ultra performance liquid chromatography (UPLC). In total, 28 compounds with high purity were obtained and their structures were comprehensively characterized by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) spectroscopy. The results demonstrate that this 2D NPLC × RPLC method with good orthogonality (58.3%) was effective for the preparative separation and purification of amide alkaloids from Piper Longum L.

  • purification of amide alkaloids from Piper Longum l using preparative two dimensional normal phase liquid chromatography reversed phase liquid chromatography
    Analyst, 2013
    Co-Authors: Wenya Zhu, Yu Jin, Xinmiao Liang
    Abstract:

    A comprehensive off-line two-dimensional liquid chromatography (2D-LC) method coupling normal phase liquid chromatography (NPLC) and reversed phase liquid chromatography (RPLC) was developed for separation and purification of amide alkaloids from Piper Longum L. In the first dimension, the crude alkaloid fractions were separated in NPLC mode and 20 fractions were collected. Then fractions 5–20 were selected for further purification in RPLC mode in the second dimension. The purities of RPLC fractions with similar structures were all identified accurately by ultra performance liquid chromatography (UPLC). In total, 28 compounds with high purity were obtained and their structures were comprehensively characterized by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) spectroscopy. The results demonstrate that this 2D NPLC × RPLC method with good orthogonality (58.3%) was effective for the preparative separation and purification of amide alkaloids from Piper Longum L.

J M Sonpetkar - One of the best experts on this subject based on the ideXlab platform.

  • Production of high titre antibody response against Russell's viper venom in mice immunized with ethanolic extract of fruits of Piper Longum L. (Piperaceae) and Piperine.
    Phytomedicine : international journal of phytotherapy and phytopharmacology, 2013
    Co-Authors: P A Shenoy, S S Nipate, J M Sonpetkar, N C Salvi, A B Waghmare, P D Chaudhari
    Abstract:

    Abstract Piper Longum L. fruits have been traditionally used against snakebites in north-eastern and southern region of India. The aim of the study was to assess the production of antibody response against Russell's viper venom in mice after prophylactic immunization with ethanolic extract of fruits of Piper Longum L. and Piperine. The mice sera were tested for the presence of antibodies against Russell's viper venom by in vitro lethality neutralization assay and in vivo lethality neutralization assay. Polyvalent anti-snake venom serum (antivenom) manufactured by Haffkine Bio-Pharmaceutical Corporation Ltd. was used as standard. Further confirmation of presence of antibodies against the venom in sera of mice immunized with PLE and Piperine was done using indirect enzyme-linked immunosorbent assay (ELISA) and double immunodiffusion test. Treatment with PLE-treated mice serum and Piperine-treated mice serum was found to inhibit the lethal action of venom both in the in vitro lethality neutralization assay and in vivo lethality neutralization assay. ELISA testing indicated that there were significantly high ( p Piper Longum and Piperine produced a high titre antibody response against Russell's viper venom in mice. The antibodies against PLE and Piperine could be useful in antivenom therapy of Russell's viper bites. PLE and Piperine may also have a potential interest in view of the development of antivenom formulations used as antidote against snake bites.

  • anti snake venom activities of ethanolic extract of fruits of Piper Longum l Piperaceae against russell s viper venom characterization of Piperine as active principle
    Journal of Ethnopharmacology, 2013
    Co-Authors: P A Shenoy, S S Nipate, J M Sonpetkar, N C Salvi, A B Waghmare, P D Chaudhari
    Abstract:

    ETHNOPHARMACOLOGICAL RELEVANCE: Piper Longum L. fruits have been traditionally used against snakebites in north-eastern and southern region of India. AIM OF THE STUDY: To examine the ability of ethanolic extract of fruits of Piper Longum L., Piperaceae (PLE) and Piperine, one of the main active principles of Piper Longum, to inhibit the Russell's viper (Doboia russelii, Viperidae) snake venom activities. MATERIALS AND METHODS: Anti-snake venom activities of ethanolic extract of fruits of Piper Longum L. (Piperaceae) and Piperine against Russell's viper venom was studied in embryonated fertile chicken eggs, mice and rats by using various models as follows: inhibition of venom lethal action, inhibition of venom haemorrhagic action (in vitro), inhibition of venom haemorrhagic action (in vivo), inhibition of venom necrotizing action, inhibition of venom defibrinogenating action, inhibition of venom induced paw edema, inhibition of venom induced mast cell degranulation, creatine kinase assay and assay for catalase activity. RESULTS: PLE was found to inhibit the venom induced haemorrhage in embryonated fertile chicken eggs. Administration of PLE and Piperine significantly (p<0.01) inhibited venom induced lethality, haemorrhage, necrosis, defibrinogenation and inflammatory paw edema in mice in a dose dependent manner. PLE and Piperine also significantly (p<0.01) reduced venom induced mast cell degranulation in rats. Venom induced decrease in catalase enzyme levels in mice kidney tissue and increase in creatine kinase enzyme levels in mice serum were significantly (p<0.01) reversed by administration of both PLE and Piperine. CONCLUSIONS: PLE possesses good anti-snake venom properties and Piperine is one of the compounds responsible for the effective venom neutralizing ability of the plant.

  • Anti-snake venom activities of ethanolic extract of fruits of Piper Longum L. (Piperaceae) against Russell's viper venom: characterization of Piperine as active principle.
    Journal of ethnopharmacology, 2013
    Co-Authors: P A Shenoy, S S Nipate, J M Sonpetkar, N C Salvi, A B Waghmare, P D Chaudhari
    Abstract:

    Abstract Ethnopharmacological relevance Piper Longum L. fruits have been traditionally used against snakebites in north-eastern and southern region of India. Aim of the study To examine the ability of ethanolic extract of fruits of Piper Longum L., Piperaceae (PLE) and Piperine, one of the main active principles of Piper Longum, to inhibit the Russell's viper (Doboia russelii, Viperidae) snake venom activities. Materials and methods Anti-snake venom activities of ethanolic extract of fruits of Piper Longum L. (Piperaceae) and Piperine against Russell's viper venom was studied in embryonated fertile chicken eggs, mice and rats by using various models as follows: inhibition of venom lethal action, inhibition of venom haemorrhagic action (in vitro), inhibition of venom haemorrhagic action (in vivo), inhibition of venom necrotizing action, inhibition of venom defibrinogenating action, inhibition of venom induced paw edema, inhibition of venom induced mast cell degranulation, creatine kinase assay and assay for catalase activity. Results PLE was found to inhibit the venom induced haemorrhage in embryonated fertile chicken eggs. Administration of PLE and Piperine significantly (p Conclusions PLE possesses good anti-snake venom properties and Piperine is one of the compounds responsible for the effective venom neutralizing ability of the plant.