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C Fusch - One of the best experts on this subject based on the ideXlab platform.

  • Physiological and pathological regulation of feto/placento/maternal leptin expression.
    Biochemical Society transactions, 2020
    Co-Authors: K Linnemann, A Malek, H Schneider, C Fusch
    Abstract:

    There is clear evidence of Placental leptin production, as shown recently in trophoblast cultures and by dual in vitro Placenta Perfusion (median production of 225 pg/min per g of tissue; 98.4% released into the maternal and 1.6% into the fetal circulation). However, the physiological impact for the mother and the fetus is unclear. The classical role of leptin is to provide information about energy stores to the central nervous system, and to reduce appetite if the energy stores are full. In pregnancy, maternal plasma leptin concentrations are elevated, and lack the well established correlation with body fat energy stores that is observed in non-pregnant women, indicating an alternative function for leptin during pregnancy and fetal development. Maternal and fetal plasma leptin levels are dysregulated in pathological conditions such as gestational diabetes, pre-eclampsia and intra-uterine growth retardation, representing an effect or a cause of disturbances in the feto/placento/maternal unit.

  • lps from bovine serum albumin drives tnf α release during ex vivo Placenta Perfusion experiments contaminates the Perfusion system but can be effectively removed by oxidative cleaning
    Placenta, 2014
    Co-Authors: Tarushika Vasanthan, N Rochow, F Mian, T Codini, B Defrance, Gerhard Fusch, Samira Samieezafarghandy, C Fusch
    Abstract:

    Introduction The dual ex-vivo Perfusion of human Placental tissue is useful to study inflammatory pathways. We found significant TNF-α release in negative controls similar in concentration to lipopolysaccharide (LPS) stimulated Placentas. The aim of the current study was to (i) identify sources driving TNF-α release and (ii) develop an approach to control for it. Method (i) To determine sources leading to TNF-α release, solutions frequently circulated through the Perfusion system and Perfusion media with different bovine serum albumin (BSA) quality were exposed to mouse macrophage cell lines (RAW264.7) and subsequently measured for TNF-α expression. (ii) To assess memory effects and validate cleaning procedures, sham Perfusion experiments were conducted either in the presence or absence of exogenous LPS, in new tubing that was contaminated, cleaned and analyzed for the effectiveness of LPS removal. Oxidative and acid-base cleaning were tested for their effectiveness to reduce LPS contamination. Results TNF-α release, observed in negative control experiments, was attributed to the use of LPS-contaminated BSA as well as inadequate cleaning of the Perfusion system. Once introduced in the Perfusion system, LPS accumulated and created a memory effect. Oxidative but not acid-base depyrogenation effectively reduced LPS levels to concentrations that were in accordance with FDA guidelines ( Discussion LPS contamination of the Placenta Perfusion model could have confounding effects on experimental outcomes leading to misinterpretation of data. To circumvent LPS contamination LPS-free BSA and oxidative depyrogenation cleaning techniques should be implemented in future Placental Perfusion studies.

  • 18 Ultrastructural Alterations of Placental Tissue After 6H of Hypoxic Dual in vitro Placenta Perfusion
    Pediatric Research, 2005
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, S Kuno, C Fusch
    Abstract:

    Background: Peripartal asphyxia affects the foetus and the Placenta with its functions. This can be studied by the dual in vitro Placenta Perfusion model enabling the separated ex vivo Perfusion of maternal and foetal circulation and simulating asphyxia. Aim: Examination of Placental villous ultrastructure by electron microscopy before and after 6h of dual in vitro Placenta Perfusion with defined hypoxia to study whether morphological alterations could indicate a loss of Placental function. Methods: Placentae (n=20) after normal pregnancies were studied. Written informed consent was obtained. Villous tissue was sampled before and after 6h of normoxic (pO2 80–90mmHg, n=10) or hypoxic (pO2= 20–30mmHg, n=10) Placenta Perfusion. Fixation, embedding, preparation of semi- and ultrathin sections. Systematic investigation by electron microscopy of terminal villous syncytio- and cytotrophoblast with its substructures mitochondria, rough and smooth endoplasmatic reticulum (ER), nucleus, golgi-apparatus and microvilli. Glucose consumption, lactate production, creatinine and antipyrin permeability as well as leptin- and hCG-release were used as control parameters during Perfusion. Results: Terminal villi of all examined Placentae showed intact substructures before and after normoxic Perfusion. After 6h of hypoxic Placenta Perfusion the terminal villi demonstrated morphological alterations: villous stroma was oedematous, cell organelles (mitochondria, ER) swollen or completely degenerated, syncytio- and cytotrophoblast interspersed with multiple vacuoles and microvilli slenderly extended or partly cut off. The foetal blood vessels appeared narrow with luminal orientated endothelial cells nuclei. The morphological results correlated with the functional data. Conclusion: 6h of hypoxic dual in vitro Placenta Perfusion induces degenerative alterations of terminal villi which may impair the Placental barrier and Placental function.

  • 18 ultrastructural alterations of Placental tissue after 6h of hypoxic dual in vitro Placenta Perfusion
    Pediatric Research, 2005
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, S Kuno, C Fusch
    Abstract:

    18 Ultrastructural Alterations of Placental Tissue After 6H of Hypoxic Dual in vitro Placenta Perfusion

  • 223 Placental transfer of the protease inhibitor nelfinavir in the dual in vitro Placenta Perfusion model
    Pediatric Research, 2005
    Co-Authors: K Linnemann, N Siebert, D Mehltretter, M Kurowski, Heyo K Kroemer, Werner Siegmund, C Fusch
    Abstract:

    223 Placental Transfer of the Protease Inhibitor Nelfinavir in the Dual in Vitro Placenta Perfusion Model

K Linnemann - One of the best experts on this subject based on the ideXlab platform.

  • Physiological and pathological regulation of feto/placento/maternal leptin expression.
    Biochemical Society transactions, 2020
    Co-Authors: K Linnemann, A Malek, H Schneider, C Fusch
    Abstract:

    There is clear evidence of Placental leptin production, as shown recently in trophoblast cultures and by dual in vitro Placenta Perfusion (median production of 225 pg/min per g of tissue; 98.4% released into the maternal and 1.6% into the fetal circulation). However, the physiological impact for the mother and the fetus is unclear. The classical role of leptin is to provide information about energy stores to the central nervous system, and to reduce appetite if the energy stores are full. In pregnancy, maternal plasma leptin concentrations are elevated, and lack the well established correlation with body fat energy stores that is observed in non-pregnant women, indicating an alternative function for leptin during pregnancy and fetal development. Maternal and fetal plasma leptin levels are dysregulated in pathological conditions such as gestational diabetes, pre-eclampsia and intra-uterine growth retardation, representing an effect or a cause of disturbances in the feto/placento/maternal unit.

  • Role of the multidrug transporter proteins ABCB1 and ABCC2 in the diaPlacental transport of talinolol in the term human Placenta.
    Drug Metabolism and Disposition, 2008
    Co-Authors: Veronika Minarikova, K Linnemann, Heyo K Kroemer, Marek Zygmunt, Christoph Fusch, Werner Siegmund
    Abstract:

    Placental syncytiotrophoblasts are known to express the efflux transporter proteins P-glycoprotein (ABCB1) and multidrug resistance-associated protein 2 (ABCC2), which are supposed to be a functional part of the human Placental barrier. With advancing gestational age, expression of ABCB1 decreases progressively, whereas ABCC2 is more expressed. To evaluate to which extent they contribute to Placental barrier function at term, permeability of talinolol, a substrate of both carriers, was measured using a validated human Placenta Perfusion model. We identified in randomized, crossover experiments a unidirectional transfer of talinolol in the fetomaternal direction because the maternofetal transfer was significantly lower (0.663 ± 0.188 versus 0.394 ± 0.067 relative to creatinine permeability, p = 0.012). Maternofetal permeability was increased by the ABCC2 inhibitor probenecid (0.59 ± 0.15 versus 0.68 ± 0.13, p = 0.028) and the nonspecific inhibitor verapamil (0.53 ± 0.09 versus 0.66 ± 0.16, p = 0.028) but was not influenced by the ABCB1 inhibitor valspodar (PSC833) (0.48 ± 0.11 versus 0.46 ± 0.09, p = 0.345). Genetic polymorphisms of ABCB1 and ABCC2 lacked significant influence on expression of the carriers and permeability of talinolol, respectively. In conclusion, maternofetal transfer of talinolol is restricted by a unidirectional process that is influenced by inhibitors of ABCC2.

  • 18 Ultrastructural Alterations of Placental Tissue After 6H of Hypoxic Dual in vitro Placenta Perfusion
    Pediatric Research, 2005
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, S Kuno, C Fusch
    Abstract:

    Background: Peripartal asphyxia affects the foetus and the Placenta with its functions. This can be studied by the dual in vitro Placenta Perfusion model enabling the separated ex vivo Perfusion of maternal and foetal circulation and simulating asphyxia. Aim: Examination of Placental villous ultrastructure by electron microscopy before and after 6h of dual in vitro Placenta Perfusion with defined hypoxia to study whether morphological alterations could indicate a loss of Placental function. Methods: Placentae (n=20) after normal pregnancies were studied. Written informed consent was obtained. Villous tissue was sampled before and after 6h of normoxic (pO2 80–90mmHg, n=10) or hypoxic (pO2= 20–30mmHg, n=10) Placenta Perfusion. Fixation, embedding, preparation of semi- and ultrathin sections. Systematic investigation by electron microscopy of terminal villous syncytio- and cytotrophoblast with its substructures mitochondria, rough and smooth endoplasmatic reticulum (ER), nucleus, golgi-apparatus and microvilli. Glucose consumption, lactate production, creatinine and antipyrin permeability as well as leptin- and hCG-release were used as control parameters during Perfusion. Results: Terminal villi of all examined Placentae showed intact substructures before and after normoxic Perfusion. After 6h of hypoxic Placenta Perfusion the terminal villi demonstrated morphological alterations: villous stroma was oedematous, cell organelles (mitochondria, ER) swollen or completely degenerated, syncytio- and cytotrophoblast interspersed with multiple vacuoles and microvilli slenderly extended or partly cut off. The foetal blood vessels appeared narrow with luminal orientated endothelial cells nuclei. The morphological results correlated with the functional data. Conclusion: 6h of hypoxic dual in vitro Placenta Perfusion induces degenerative alterations of terminal villi which may impair the Placental barrier and Placental function.

  • 18 ultrastructural alterations of Placental tissue after 6h of hypoxic dual in vitro Placenta Perfusion
    Pediatric Research, 2005
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, S Kuno, C Fusch
    Abstract:

    18 Ultrastructural Alterations of Placental Tissue After 6H of Hypoxic Dual in vitro Placenta Perfusion

  • 223 Placental transfer of the protease inhibitor nelfinavir in the dual in vitro Placenta Perfusion model
    Pediatric Research, 2005
    Co-Authors: K Linnemann, N Siebert, D Mehltretter, M Kurowski, Heyo K Kroemer, Werner Siegmund, C Fusch
    Abstract:

    223 Placental Transfer of the Protease Inhibitor Nelfinavir in the Dual in Vitro Placenta Perfusion Model

N Siebert - One of the best experts on this subject based on the ideXlab platform.

  • 18 Ultrastructural Alterations of Placental Tissue After 6H of Hypoxic Dual in vitro Placenta Perfusion
    Pediatric Research, 2005
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, S Kuno, C Fusch
    Abstract:

    Background: Peripartal asphyxia affects the foetus and the Placenta with its functions. This can be studied by the dual in vitro Placenta Perfusion model enabling the separated ex vivo Perfusion of maternal and foetal circulation and simulating asphyxia. Aim: Examination of Placental villous ultrastructure by electron microscopy before and after 6h of dual in vitro Placenta Perfusion with defined hypoxia to study whether morphological alterations could indicate a loss of Placental function. Methods: Placentae (n=20) after normal pregnancies were studied. Written informed consent was obtained. Villous tissue was sampled before and after 6h of normoxic (pO2 80–90mmHg, n=10) or hypoxic (pO2= 20–30mmHg, n=10) Placenta Perfusion. Fixation, embedding, preparation of semi- and ultrathin sections. Systematic investigation by electron microscopy of terminal villous syncytio- and cytotrophoblast with its substructures mitochondria, rough and smooth endoplasmatic reticulum (ER), nucleus, golgi-apparatus and microvilli. Glucose consumption, lactate production, creatinine and antipyrin permeability as well as leptin- and hCG-release were used as control parameters during Perfusion. Results: Terminal villi of all examined Placentae showed intact substructures before and after normoxic Perfusion. After 6h of hypoxic Placenta Perfusion the terminal villi demonstrated morphological alterations: villous stroma was oedematous, cell organelles (mitochondria, ER) swollen or completely degenerated, syncytio- and cytotrophoblast interspersed with multiple vacuoles and microvilli slenderly extended or partly cut off. The foetal blood vessels appeared narrow with luminal orientated endothelial cells nuclei. The morphological results correlated with the functional data. Conclusion: 6h of hypoxic dual in vitro Placenta Perfusion induces degenerative alterations of terminal villi which may impair the Placental barrier and Placental function.

  • 18 ultrastructural alterations of Placental tissue after 6h of hypoxic dual in vitro Placenta Perfusion
    Pediatric Research, 2005
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, S Kuno, C Fusch
    Abstract:

    18 Ultrastructural Alterations of Placental Tissue After 6H of Hypoxic Dual in vitro Placenta Perfusion

  • 223 Placental transfer of the protease inhibitor nelfinavir in the dual in vitro Placenta Perfusion model
    Pediatric Research, 2005
    Co-Authors: K Linnemann, N Siebert, D Mehltretter, M Kurowski, Heyo K Kroemer, Werner Siegmund, C Fusch
    Abstract:

    223 Placental Transfer of the Protease Inhibitor Nelfinavir in the Dual in Vitro Placenta Perfusion Model

  • 223 Placental Transfer of the Protease Inhibitor Nelfinavir in the Dual in Vitro Placenta Perfusion Model
    Pediatric Research, 2005
    Co-Authors: K Linnemann, N Siebert, D Mehltretter, M Kurowski, Heyo K Kroemer, Werner Siegmund, C Fusch
    Abstract:

    Intro: The protease inhibitor nelfinavir is a common drug for treatment of HIV in pregnancy. Pairs of serum samples of maternal vein - and umbilical cord blood show very low levels of nelfinavir in umbilical cord blood. These results could be caused by an backward efflux of Nelfinavir into the maternal circulation by p-glycoprotein, which is highly expressed in the Placenta. Nelfinavir is a known substrate of p-glycoprotein. Aim: Measurement of Placental transfer of nelfinavir and determination of an active transport Material and Methods: Placentas were obtained after written informed consent. Dual closed loop in vitro Perfusion of isolated cotyledons (n=12): Perfusion experiments in 4 phases: 1. stabilization and control phase (1h); 2. addition of nelfinavir into first circuit (either maternal or fetal) and measurement of nelfinavir transfer (3h); 3. washout phase (0.5h) followed by; 4. addition of nelfinavir into the second circuit. The order of nelfinavir administration (first maternal or first fetal) was randomized. These transfer data were normalized with the antipyrine permeability to correct for diffusional differences between the experiments. Control parameters for vital Placental tissue were glucose consumption, lactate production, leptin production and permeability of creatinine and antipyrine. Results: The permeability ratio of nelfinavir:antipyrine in materno-fetal direction was not significant different from the permeability ratio in fetal-maternal direction (0.5±0.5 v 0.3±0.4). Glucose consumption (0.23±0.07 μmol/g/min), lactate production (0.42±0.12 μmol/g/min) and leptin release (225pg/g/min) indicated a normal metabolism of the tissue. Feto-maternal leakage was below 4ml/h. Conclusion: It is unlikely that active directional transporters are involved in the Placental transfer of nelfinavir. The observed low fetal serum concentrations in treated pregnancies are rather caused by other mechanisms like high plasma protein binding or drug metabolism.

  • 13 Ultrastructure of Placental Tissue after 6H of Dual In Vitro Placenta Perfusion
    Pediatric Research, 2004
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, C Fusch
    Abstract:

    Background: The dual in vitro Placenta Perfusion model allows the separated ex vivo Perfusion of the maternal & fetal circulation and the investigation of the different functions of the Placenta. The vitality and integrity of the perfused tissue up to the end of the Perfusion experiment is essential for valid experiments. There is no systematic study about the ultrastructural integrity of the perfused Placental tissue. Aim of the investigation: Estimation of Placental ultrastructural intergrity after 6h of Perfusion for extended evaluation of the dual in vitro Placenta Perfusion model using electron microscopy. Methods: Placentas (n=10) after uncomplicated pregnacies at term, the mothers gave their written informed consent. Sampling of villous tissue before and after 6h of dual in vitro Placenta Perfusion and deposition in fixation buffer. Preparation of semithin and ultrathin sections. Systematic investigation of the substructures rough and smooth endoplasmatic reticulum (ER), mitochondrium, nucleus, mikrovilli of syncytio- and cytotrophoblast by a blinded investigator. Control parameters for Placental function: glucose consumption, lactat production, creatinine- and antipyrin permeability as well as leptin- and hCG release. Results: We found no significant differences of the substructures before and after 6h of dual in vitro Placenta Perfusion with stable Placental function (constant glucose consumption and hormone release). We found in Perfusion experiments with poor Placental function (glucose consumption

N Bachmaier - One of the best experts on this subject based on the ideXlab platform.

  • 18 Ultrastructural Alterations of Placental Tissue After 6H of Hypoxic Dual in vitro Placenta Perfusion
    Pediatric Research, 2005
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, S Kuno, C Fusch
    Abstract:

    Background: Peripartal asphyxia affects the foetus and the Placenta with its functions. This can be studied by the dual in vitro Placenta Perfusion model enabling the separated ex vivo Perfusion of maternal and foetal circulation and simulating asphyxia. Aim: Examination of Placental villous ultrastructure by electron microscopy before and after 6h of dual in vitro Placenta Perfusion with defined hypoxia to study whether morphological alterations could indicate a loss of Placental function. Methods: Placentae (n=20) after normal pregnancies were studied. Written informed consent was obtained. Villous tissue was sampled before and after 6h of normoxic (pO2 80–90mmHg, n=10) or hypoxic (pO2= 20–30mmHg, n=10) Placenta Perfusion. Fixation, embedding, preparation of semi- and ultrathin sections. Systematic investigation by electron microscopy of terminal villous syncytio- and cytotrophoblast with its substructures mitochondria, rough and smooth endoplasmatic reticulum (ER), nucleus, golgi-apparatus and microvilli. Glucose consumption, lactate production, creatinine and antipyrin permeability as well as leptin- and hCG-release were used as control parameters during Perfusion. Results: Terminal villi of all examined Placentae showed intact substructures before and after normoxic Perfusion. After 6h of hypoxic Placenta Perfusion the terminal villi demonstrated morphological alterations: villous stroma was oedematous, cell organelles (mitochondria, ER) swollen or completely degenerated, syncytio- and cytotrophoblast interspersed with multiple vacuoles and microvilli slenderly extended or partly cut off. The foetal blood vessels appeared narrow with luminal orientated endothelial cells nuclei. The morphological results correlated with the functional data. Conclusion: 6h of hypoxic dual in vitro Placenta Perfusion induces degenerative alterations of terminal villi which may impair the Placental barrier and Placental function.

  • 18 ultrastructural alterations of Placental tissue after 6h of hypoxic dual in vitro Placenta Perfusion
    Pediatric Research, 2005
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, S Kuno, C Fusch
    Abstract:

    18 Ultrastructural Alterations of Placental Tissue After 6H of Hypoxic Dual in vitro Placenta Perfusion

  • 13 Ultrastructure of Placental Tissue after 6H of Dual In Vitro Placenta Perfusion
    Pediatric Research, 2004
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, C Fusch
    Abstract:

    Background: The dual in vitro Placenta Perfusion model allows the separated ex vivo Perfusion of the maternal & fetal circulation and the investigation of the different functions of the Placenta. The vitality and integrity of the perfused tissue up to the end of the Perfusion experiment is essential for valid experiments. There is no systematic study about the ultrastructural integrity of the perfused Placental tissue. Aim of the investigation: Estimation of Placental ultrastructural intergrity after 6h of Perfusion for extended evaluation of the dual in vitro Placenta Perfusion model using electron microscopy. Methods: Placentas (n=10) after uncomplicated pregnacies at term, the mothers gave their written informed consent. Sampling of villous tissue before and after 6h of dual in vitro Placenta Perfusion and deposition in fixation buffer. Preparation of semithin and ultrathin sections. Systematic investigation of the substructures rough and smooth endoplasmatic reticulum (ER), mitochondrium, nucleus, mikrovilli of syncytio- and cytotrophoblast by a blinded investigator. Control parameters for Placental function: glucose consumption, lactat production, creatinine- and antipyrin permeability as well as leptin- and hCG release. Results: We found no significant differences of the substructures before and after 6h of dual in vitro Placenta Perfusion with stable Placental function (constant glucose consumption and hormone release). We found in Perfusion experiments with poor Placental function (glucose consumption

  • 13 ultrastructure of Placental tissue after 6h of dual in vitro Placenta Perfusion
    Pediatric Research, 2004
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, C Fusch
    Abstract:

    Background: The dual in vitro Placenta Perfusion model allows the separated ex vivo Perfusion of the maternal & fetal circulation and the investigation of the different functions of the Placenta. The vitality and integrity of the perfused tissue up to the end of the Perfusion experiment is essential for valid experiments. There is no systematic study about the ultrastructural integrity of the perfused Placental tissue. Aim of the investigation: Estimation of Placental ultrastructural intergrity after 6h of Perfusion for extended evaluation of the dual in vitro Placenta Perfusion model using electron microscopy.

R Warzok - One of the best experts on this subject based on the ideXlab platform.

  • 18 Ultrastructural Alterations of Placental Tissue After 6H of Hypoxic Dual in vitro Placenta Perfusion
    Pediatric Research, 2005
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, S Kuno, C Fusch
    Abstract:

    Background: Peripartal asphyxia affects the foetus and the Placenta with its functions. This can be studied by the dual in vitro Placenta Perfusion model enabling the separated ex vivo Perfusion of maternal and foetal circulation and simulating asphyxia. Aim: Examination of Placental villous ultrastructure by electron microscopy before and after 6h of dual in vitro Placenta Perfusion with defined hypoxia to study whether morphological alterations could indicate a loss of Placental function. Methods: Placentae (n=20) after normal pregnancies were studied. Written informed consent was obtained. Villous tissue was sampled before and after 6h of normoxic (pO2 80–90mmHg, n=10) or hypoxic (pO2= 20–30mmHg, n=10) Placenta Perfusion. Fixation, embedding, preparation of semi- and ultrathin sections. Systematic investigation by electron microscopy of terminal villous syncytio- and cytotrophoblast with its substructures mitochondria, rough and smooth endoplasmatic reticulum (ER), nucleus, golgi-apparatus and microvilli. Glucose consumption, lactate production, creatinine and antipyrin permeability as well as leptin- and hCG-release were used as control parameters during Perfusion. Results: Terminal villi of all examined Placentae showed intact substructures before and after normoxic Perfusion. After 6h of hypoxic Placenta Perfusion the terminal villi demonstrated morphological alterations: villous stroma was oedematous, cell organelles (mitochondria, ER) swollen or completely degenerated, syncytio- and cytotrophoblast interspersed with multiple vacuoles and microvilli slenderly extended or partly cut off. The foetal blood vessels appeared narrow with luminal orientated endothelial cells nuclei. The morphological results correlated with the functional data. Conclusion: 6h of hypoxic dual in vitro Placenta Perfusion induces degenerative alterations of terminal villi which may impair the Placental barrier and Placental function.

  • 18 ultrastructural alterations of Placental tissue after 6h of hypoxic dual in vitro Placenta Perfusion
    Pediatric Research, 2005
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, S Kuno, C Fusch
    Abstract:

    18 Ultrastructural Alterations of Placental Tissue After 6H of Hypoxic Dual in vitro Placenta Perfusion

  • 13 Ultrastructure of Placental Tissue after 6H of Dual In Vitro Placenta Perfusion
    Pediatric Research, 2004
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, C Fusch
    Abstract:

    Background: The dual in vitro Placenta Perfusion model allows the separated ex vivo Perfusion of the maternal & fetal circulation and the investigation of the different functions of the Placenta. The vitality and integrity of the perfused tissue up to the end of the Perfusion experiment is essential for valid experiments. There is no systematic study about the ultrastructural integrity of the perfused Placental tissue. Aim of the investigation: Estimation of Placental ultrastructural intergrity after 6h of Perfusion for extended evaluation of the dual in vitro Placenta Perfusion model using electron microscopy. Methods: Placentas (n=10) after uncomplicated pregnacies at term, the mothers gave their written informed consent. Sampling of villous tissue before and after 6h of dual in vitro Placenta Perfusion and deposition in fixation buffer. Preparation of semithin and ultrathin sections. Systematic investigation of the substructures rough and smooth endoplasmatic reticulum (ER), mitochondrium, nucleus, mikrovilli of syncytio- and cytotrophoblast by a blinded investigator. Control parameters for Placental function: glucose consumption, lactat production, creatinine- and antipyrin permeability as well as leptin- and hCG release. Results: We found no significant differences of the substructures before and after 6h of dual in vitro Placenta Perfusion with stable Placental function (constant glucose consumption and hormone release). We found in Perfusion experiments with poor Placental function (glucose consumption

  • 13 ultrastructure of Placental tissue after 6h of dual in vitro Placenta Perfusion
    Pediatric Research, 2004
    Co-Authors: N Bachmaier, K Linnemann, R Warzok, N Siebert, C Fusch
    Abstract:

    Background: The dual in vitro Placenta Perfusion model allows the separated ex vivo Perfusion of the maternal & fetal circulation and the investigation of the different functions of the Placenta. The vitality and integrity of the perfused tissue up to the end of the Perfusion experiment is essential for valid experiments. There is no systematic study about the ultrastructural integrity of the perfused Placental tissue. Aim of the investigation: Estimation of Placental ultrastructural intergrity after 6h of Perfusion for extended evaluation of the dual in vitro Placenta Perfusion model using electron microscopy.