Plant Hormone

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 33444 Experts worldwide ranked by ideXlab platform

Chenzhu Wang - One of the best experts on this subject based on the ideXlab platform.

  • a gustatory receptor tuned to the steroid Plant Hormone brassinolide in plutella xylostella lepidoptera plutellidae
    eLife, 2020
    Co-Authors: Ke Yang, Xinlin Gong, Lingqiao Huang, Chao Ning, Chenzhu Wang
    Abstract:

    Feeding and oviposition deterrents help phytophagous insects to identify host Plants. The taste organs of phytophagous insects contain bitter gustatory receptors (GRs). To explore their function, the GRs in Plutella xylostella were analyzed. Through RNA sequencing and qPCR, we detected abundant PxylGr34 transcripts in the larval head and adult antennae. Functional analyses using the Xenopus oocyte expression system and 24 diverse phytochemicals showed that PxylGr34 is tuned to the canonical Plant Hormones brassinolide (BL) and 24-epibrassinolide (EBL). Electrophysiological analyses revealed that the medial sensilla styloconica of 4th instar larvae are responsive to BL and EBL. Dual-choice bioassays demonstrated that BL inhibits larval feeding and female oviposition. Knock-down of PxylGr34 by RNAi attenuates the taste responses to BL, and abolishes BL-induced feeding inhibition. These results increase our understanding of how herbivorous insects detect compounds that deter feeding and oviposition, and may be useful for designing Plant Hormone-based pest management strategies.

  • a gustatory receptor tuned to the steroid Plant Hormone brassinolide in plutella xylostella lepidoptera plutellidae
    bioRxiv, 2020
    Co-Authors: Ke Yang, Xinlin Gong, Lingqiao Huang, Chao Ning, Chenzhu Wang
    Abstract:

    Abstract Feeding and oviposition deterrents help phytophagous insects to identify host Plants. The taste organs of phytophagous insects contain bitter gustatory receptors (GRs). To explore their function, we focused on PxylGr34, a bitter GR in Plutella xylostella (L.). We detected abundant PxylGr34 transcripts in the larval head and specific expression of PxylGr34 in the antennae of females. Analyses using the Xenopus oocyte expression system and two-electrode voltage-clamp recording showed that PxylGr34 is specifically tuned to the Plant Hormone brassinolide (BL) and its analog 24-epibrassinolide. Electrophysiological analyses revealed that the medial sensilla styloconica on the maxillary galea of the 4th instar larvae are responsive to BL. Dual-choice bioassays demonstrated that BL inhibits larval feeding and female ovipositing. Knock-down of PxylGr34 by RNAi abolished BL-induced feeding inhibition. These results shed light on gustatory coding mechanisms and deterrence of insect feeding and ovipositing, and may be useful for designing Plant Hormone-based pest management strategies.

Johan N. Jansonius - One of the best experts on this subject based on the ideXlab platform.

  • Structure of 1-aminocyclopropane-1-carboxylate synthase, a key enzyme in the biosynthesis of the Plant Hormone ethylene
    Journal of Molecular Biology, 1999
    Co-Authors: Guido Capitani, Jack F. Kirsch, Paola Storici, Liang Feng, Erhard Hohenester, Johan N. Jansonius
    Abstract:

    The 2.4 Å crystal structure of the vitamin B6-dependent enzyme 1-aminocyclopropane-1-carboxylate (ACC) synthase is described. This enzyme catalyses the committed step in the biosynthesis of ethylene, a Plant Hormone that is responsible for the initiation of fruit ripening and for regulating many other developmental processes. ACC synthase has 15% sequence identity with the well-studied aspartate aminotransferase, and a completely different catalytic activity yet the overall folds and the active sites are very similar. The new structure together with available biochemical data enables a comparative mechanistic analysis that largely explains the catalytic roles of the conserved and non-conserved active site residues. An external aldimine reaction intermediate (external aldimine with ACC, i.e. with the product) has been modeled. The new structure provides a basis for the rational design of inhibitors with broad agricultural applications.

Ke Yang - One of the best experts on this subject based on the ideXlab platform.

  • a gustatory receptor tuned to the steroid Plant Hormone brassinolide in plutella xylostella lepidoptera plutellidae
    eLife, 2020
    Co-Authors: Ke Yang, Xinlin Gong, Lingqiao Huang, Chao Ning, Chenzhu Wang
    Abstract:

    Feeding and oviposition deterrents help phytophagous insects to identify host Plants. The taste organs of phytophagous insects contain bitter gustatory receptors (GRs). To explore their function, the GRs in Plutella xylostella were analyzed. Through RNA sequencing and qPCR, we detected abundant PxylGr34 transcripts in the larval head and adult antennae. Functional analyses using the Xenopus oocyte expression system and 24 diverse phytochemicals showed that PxylGr34 is tuned to the canonical Plant Hormones brassinolide (BL) and 24-epibrassinolide (EBL). Electrophysiological analyses revealed that the medial sensilla styloconica of 4th instar larvae are responsive to BL and EBL. Dual-choice bioassays demonstrated that BL inhibits larval feeding and female oviposition. Knock-down of PxylGr34 by RNAi attenuates the taste responses to BL, and abolishes BL-induced feeding inhibition. These results increase our understanding of how herbivorous insects detect compounds that deter feeding and oviposition, and may be useful for designing Plant Hormone-based pest management strategies.

  • a gustatory receptor tuned to the steroid Plant Hormone brassinolide in plutella xylostella lepidoptera plutellidae
    bioRxiv, 2020
    Co-Authors: Ke Yang, Xinlin Gong, Lingqiao Huang, Chao Ning, Chenzhu Wang
    Abstract:

    Abstract Feeding and oviposition deterrents help phytophagous insects to identify host Plants. The taste organs of phytophagous insects contain bitter gustatory receptors (GRs). To explore their function, we focused on PxylGr34, a bitter GR in Plutella xylostella (L.). We detected abundant PxylGr34 transcripts in the larval head and specific expression of PxylGr34 in the antennae of females. Analyses using the Xenopus oocyte expression system and two-electrode voltage-clamp recording showed that PxylGr34 is specifically tuned to the Plant Hormone brassinolide (BL) and its analog 24-epibrassinolide. Electrophysiological analyses revealed that the medial sensilla styloconica on the maxillary galea of the 4th instar larvae are responsive to BL. Dual-choice bioassays demonstrated that BL inhibits larval feeding and female ovipositing. Knock-down of PxylGr34 by RNAi abolished BL-induced feeding inhibition. These results shed light on gustatory coding mechanisms and deterrence of insect feeding and ovipositing, and may be useful for designing Plant Hormone-based pest management strategies.

Guido Capitani - One of the best experts on this subject based on the ideXlab platform.

  • Structure of 1-aminocyclopropane-1-carboxylate synthase, a key enzyme in the biosynthesis of the Plant Hormone ethylene
    Journal of Molecular Biology, 1999
    Co-Authors: Guido Capitani, Jack F. Kirsch, Paola Storici, Liang Feng, Erhard Hohenester, Johan N. Jansonius
    Abstract:

    The 2.4 Å crystal structure of the vitamin B6-dependent enzyme 1-aminocyclopropane-1-carboxylate (ACC) synthase is described. This enzyme catalyses the committed step in the biosynthesis of ethylene, a Plant Hormone that is responsible for the initiation of fruit ripening and for regulating many other developmental processes. ACC synthase has 15% sequence identity with the well-studied aspartate aminotransferase, and a completely different catalytic activity yet the overall folds and the active sites are very similar. The new structure together with available biochemical data enables a comparative mechanistic analysis that largely explains the catalytic roles of the conserved and non-conserved active site residues. An external aldimine reaction intermediate (external aldimine with ACC, i.e. with the product) has been modeled. The new structure provides a basis for the rational design of inhibitors with broad agricultural applications.

Chao Ning - One of the best experts on this subject based on the ideXlab platform.

  • a gustatory receptor tuned to the steroid Plant Hormone brassinolide in plutella xylostella lepidoptera plutellidae
    eLife, 2020
    Co-Authors: Ke Yang, Xinlin Gong, Lingqiao Huang, Chao Ning, Chenzhu Wang
    Abstract:

    Feeding and oviposition deterrents help phytophagous insects to identify host Plants. The taste organs of phytophagous insects contain bitter gustatory receptors (GRs). To explore their function, the GRs in Plutella xylostella were analyzed. Through RNA sequencing and qPCR, we detected abundant PxylGr34 transcripts in the larval head and adult antennae. Functional analyses using the Xenopus oocyte expression system and 24 diverse phytochemicals showed that PxylGr34 is tuned to the canonical Plant Hormones brassinolide (BL) and 24-epibrassinolide (EBL). Electrophysiological analyses revealed that the medial sensilla styloconica of 4th instar larvae are responsive to BL and EBL. Dual-choice bioassays demonstrated that BL inhibits larval feeding and female oviposition. Knock-down of PxylGr34 by RNAi attenuates the taste responses to BL, and abolishes BL-induced feeding inhibition. These results increase our understanding of how herbivorous insects detect compounds that deter feeding and oviposition, and may be useful for designing Plant Hormone-based pest management strategies.

  • a gustatory receptor tuned to the steroid Plant Hormone brassinolide in plutella xylostella lepidoptera plutellidae
    bioRxiv, 2020
    Co-Authors: Ke Yang, Xinlin Gong, Lingqiao Huang, Chao Ning, Chenzhu Wang
    Abstract:

    Abstract Feeding and oviposition deterrents help phytophagous insects to identify host Plants. The taste organs of phytophagous insects contain bitter gustatory receptors (GRs). To explore their function, we focused on PxylGr34, a bitter GR in Plutella xylostella (L.). We detected abundant PxylGr34 transcripts in the larval head and specific expression of PxylGr34 in the antennae of females. Analyses using the Xenopus oocyte expression system and two-electrode voltage-clamp recording showed that PxylGr34 is specifically tuned to the Plant Hormone brassinolide (BL) and its analog 24-epibrassinolide. Electrophysiological analyses revealed that the medial sensilla styloconica on the maxillary galea of the 4th instar larvae are responsive to BL. Dual-choice bioassays demonstrated that BL inhibits larval feeding and female ovipositing. Knock-down of PxylGr34 by RNAi abolished BL-induced feeding inhibition. These results shed light on gustatory coding mechanisms and deterrence of insect feeding and ovipositing, and may be useful for designing Plant Hormone-based pest management strategies.

Plant Hormone - 15 days free trial to Access Experts & Abstracts