The Experts below are selected from a list of 6894 Experts worldwide ranked by ideXlab platform
Siwon Lee - One of the best experts on this subject based on the ideXlab platform.
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The Plant Pathology Journal Note Open Access Development and Practical Use of RT-PCR for Seed-transmitted Prune dwarf virus in Quarantine
2016Co-Authors: Siwon Lee, Yonggil ShinAbstract:Among imported Plants, seeds are the items that have many latent pathogens and are difficult to inspect. Also, they are the import and export items whose market is expected to expand. The biggest problem with seeds is viruses. Prune dwarf virus (PDV) is the virus that is commonly inspected in Prunus cerasifera, P. persica, P. armeniaca, P. mandshurica, P. cerasus, P. avium or P. serotina seeds. In this study, two RT-PCR primer sets, which can promptly and specifically diagnose Plant Quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The method developed in this study has detected PDV in 18 cases since 2007, and is expected to continuously contribute to the Plant Quarantine in Korea
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development and verification of nested pcr assay for detection of tobacco rattle virus in Plant Quarantine
Journal of Bacteriology and Virology, 2015Co-Authors: Siwon Lee, Jinyoung Lee, Suheon Lee, Taeyoung AhnAbstract:Tobacco rattle virus (TRV) is a Plant pathogen belonging to the Group IV positive-sense single-stranded RNA viruses. TRV causes disease in various Plants (e.g., potato, tomato and tobacco), for which it was classified as a controlled Quarantine virus in Korea. This study aimed to develop specific primer sets for the rapid detection of TRV. Two RT-PCR primer sets were developed for specific detection of TRV. Furthermore, nested primer sets were also developed, which is required for high sensitivity detection in Plant Quarantine. The RT-PCR and nested PCR products had the following sizes: set 5 (1,096→540 bp), and set 7 (878→756 bp), respectively. In addition, a modified positive-control plasmid was also developed for use as a positive control in TRV Quarantine. The diagnostic system for TRV detection was verified using samples from Korean Quarantine sites for the last five years (2009-2014). A total of 83 cases were detected among various import crops. This system for detection of TRV will continuously contribute to Plant Quarantine in the future.
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development and practical use of rt pcr for seed transmitted prune dwarf virus in Quarantine
Plant Pathology Journal, 2014Co-Authors: Siwon Lee, Yonggil ShinAbstract:Among imported Plants, seeds are the items that have many latent pathogens and are difficult to inspect. Also, they are the import and export items whose market is expected to expand. The biggest problem with seeds is viruses. Prune dwarf virus (PDV) is the virus that is commonly inspected in Prunus cerasifera, P. persica, P. armeniaca, P. mandshurica, P. cerasus, P. avium or P. serotina seeds. In this study, two RT-PCR primer sets, which can promptly and specifically diagnose Plant Quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The method developed in this study has detected PDV in 18 cases since 2007, and is expected to continuously contribute to the Plant Quarantine in Korea.
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development of pcr diagnosis system for Plant Quarantine seed borne wheat streak mosaic virus
The Korean Journal of Microbiology, 2013Co-Authors: Siwon Lee, Eunha Kang, Yeonmee Chu, Yonggil Shin, Taeyoung AhnAbstract:Wheat streak mosaic virus (WSMV), a member of the genus Tritimovirus in Potyviridae, severely impacts wheat and corn seed worldwide, but has yet to be detected in Korea, and hence, every effort should be made to prevent its introduction. To prevent WSMV from entering the country, it is necessary to prepare a specific, sensitive, simple, and fast detection method for routine application to Plant Quarantine procedures. For this reason, a two-step diagnosis system consisting of RT-PCR and nested PCR is being used for WSMV detection. In addition, a novel positive control was developed for use with the system. WSMV has been detected in seed sweet corn from Japan and seed wheat from USA by a two-step diagnosis system, the details of which are described in this study. After sequence analysis, similarities of 80.6 and 100.0% with other isolates were determined by BLAST. They showed the same topology, which was classified as 4 genotypes by various phylogenetic trees, using a poly protein encoding sequence amplification. In this analysis, WSMV-JSweet-corn2868 (JX845574) is classified as clade B, while WSMV-Uwheat1944-1 (KC754959) and WSMV-Uwheat1944-2 (KC754960) belong to clade D.
Yonggil Shin - One of the best experts on this subject based on the ideXlab platform.
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The Plant Pathology Journal Note Open Access Development and Practical Use of RT-PCR for Seed-transmitted Prune dwarf virus in Quarantine
2016Co-Authors: Siwon Lee, Yonggil ShinAbstract:Among imported Plants, seeds are the items that have many latent pathogens and are difficult to inspect. Also, they are the import and export items whose market is expected to expand. The biggest problem with seeds is viruses. Prune dwarf virus (PDV) is the virus that is commonly inspected in Prunus cerasifera, P. persica, P. armeniaca, P. mandshurica, P. cerasus, P. avium or P. serotina seeds. In this study, two RT-PCR primer sets, which can promptly and specifically diagnose Plant Quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The method developed in this study has detected PDV in 18 cases since 2007, and is expected to continuously contribute to the Plant Quarantine in Korea
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development and practical use of rt pcr for seed transmitted prune dwarf virus in Quarantine
Plant Pathology Journal, 2014Co-Authors: Siwon Lee, Yonggil ShinAbstract:Among imported Plants, seeds are the items that have many latent pathogens and are difficult to inspect. Also, they are the import and export items whose market is expected to expand. The biggest problem with seeds is viruses. Prune dwarf virus (PDV) is the virus that is commonly inspected in Prunus cerasifera, P. persica, P. armeniaca, P. mandshurica, P. cerasus, P. avium or P. serotina seeds. In this study, two RT-PCR primer sets, which can promptly and specifically diagnose Plant Quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The method developed in this study has detected PDV in 18 cases since 2007, and is expected to continuously contribute to the Plant Quarantine in Korea.
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development of pcr diagnosis system for Plant Quarantine seed borne wheat streak mosaic virus
The Korean Journal of Microbiology, 2013Co-Authors: Siwon Lee, Eunha Kang, Yeonmee Chu, Yonggil Shin, Taeyoung AhnAbstract:Wheat streak mosaic virus (WSMV), a member of the genus Tritimovirus in Potyviridae, severely impacts wheat and corn seed worldwide, but has yet to be detected in Korea, and hence, every effort should be made to prevent its introduction. To prevent WSMV from entering the country, it is necessary to prepare a specific, sensitive, simple, and fast detection method for routine application to Plant Quarantine procedures. For this reason, a two-step diagnosis system consisting of RT-PCR and nested PCR is being used for WSMV detection. In addition, a novel positive control was developed for use with the system. WSMV has been detected in seed sweet corn from Japan and seed wheat from USA by a two-step diagnosis system, the details of which are described in this study. After sequence analysis, similarities of 80.6 and 100.0% with other isolates were determined by BLAST. They showed the same topology, which was classified as 4 genotypes by various phylogenetic trees, using a poly protein encoding sequence amplification. In this analysis, WSMV-JSweet-corn2868 (JX845574) is classified as clade B, while WSMV-Uwheat1944-1 (KC754959) and WSMV-Uwheat1944-2 (KC754960) belong to clade D.
Shanhong Yu - One of the best experts on this subject based on the ideXlab platform.
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real time fluorescence pcr method for detection of burkholderia glumae from rice
Rice Science, 2009Co-Authors: Yuan Fang, Yan Huai, Wenxiao Tian, Lihui Xu, Shanhong YuAbstract:Abstract Burkholderia glumae causing seedling rot and grain rot of rice was listed as a Plant Quarantine disease of China in 2007. It's quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed Quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×104 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. B. glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1:100.
Kenji Kasugai - One of the best experts on this subject based on the ideXlab platform.
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Sampling inspection to prevent the invasion of alien pests: statistical theory of import Plant Quarantine systems in Japan
Population Ecology, 2016Co-Authors: Kohji Yamamura, Hajime Katsumata, Junji Yoshioka, Tatsuya Yuda, Kenji KasugaiAbstract:The establishment of appropriate import Quarantine systems is the best known method for preventing the unintentional introduction of invasive alien pests. However, Quarantine systems are sometimes judged as non-tariff barriers against trade by the World Trade Organization. The construction of a common scientific theory for Quarantine systems is thus extremely important to prevent invasion without causing international conflict. We explain several statistical theories that have been adopted in import Plant Quarantine systems in Japan. Quarantine systems include three major components: (1) import sampling inspection, (2) early detection procedures, and (3) emergency control. We first explain the principle of risk management that was commonly adopted in these components. Then, we explain the method for calculating the required sample size in the import sampling inspection. We then explain hierarchical sampling inspection for detecting alien pests inside Japan. We further explain the theory for declaring the eradication of invasive alien pests as an emergency control. Actual examples of Quarantine actions against the invasion of plum pox virus disease and citrus huanglongbing are discussed.
Zhihong Li - One of the best experts on this subject based on the ideXlab platform.
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global establishment risk of economically important fruit fly species tephritidae
PLOS ONE, 2015Co-Authors: Dean R Paini, Cong Wang, Yan Fang, Zhihong LiAbstract:The global invasion of Tephritidae (fruit flies) attracts a great deal of attention in the field of Plant Quarantine and invasion biology because of their economic importance. Predicting which one in hundreds of potential invasive fruit fly species is most likely to establish in a region presents a significant challenge, but can be facilitated using a self organising map (SOM), which is able to analyse species associations to rank large numbers of species simultaneously with an index of establishment. A global presence/absence dataset including 180 economically significant fruit fly species in 118 countries was analysed using a SOM. We compare and contrast ranked lists from six countries selected from each continent, and also show that those countries geographically close were clustered together by the SOM analysis because they have similar fruit fly assemblages. These closely clustered countries therefore represent greater threats to each other as sources of invasive fruit fly species. Finally, we indicate how this SOM method could be utilized as an initial screen to support prioritizing fruit fly species for further research into their potential to invade a region.