The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Czeslaw Lugowski - One of the best experts on this subject based on the ideXlab platform.
-
the o antigen of Plesiomonas shigelloides serotype o36 containing pseudaminic acid
Carbohydrate Research, 2016Co-Authors: Marta Kaszowska, Katarina Stojkovic, Tomasz Niedziela, Czeslaw LugowskiAbstract:Abstract The structure of the repeating unit of O-antigen of Plesiomonas shigelloides serotype O36 has been investigated by 1H and 13C NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and chemical methods. The new structure of trisaccharide has been established: →4)-β-Pse5Ac7(R3Hb)-(2 → 4)-β-D-Galp-(1 → 3)-β-D-GlcpNAc-(1→ These trisaccharide O-antigen units substitute the core undecasaccharide at C-4 of the β-D-GlcpNAc residue. The core oligosaccharide and lipid A are identical with these of the serotype O17 (PCM 2231) (Maciejewska, A., Lukasiewicz, J., Kaszowska, M., Jachymek, W., Man-Kupisinska, A.; Lugowski, C. Mar. Drugs. 2013, 11 (2), 440–454; Lukasiewicz, J., Dzieciatkowska, M., Niedziela, T., Jachymek, W., Augustyniuk, A., Kenne, L., Lugowski, C. Biochemistry, 2006, 45, 10434–10447).
-
the o antigen of Plesiomonas shigelloides serotype o36 containing pseudaminic acid
Carbohydrate Research, 2016Co-Authors: Marta Kaszowska, Katarina Stojkovic, Tomasz Niedziela, Czeslaw LugowskiAbstract:The structure of the repeating unit of O-antigen of Plesiomonas shigelloides serotype O36 has been investigated by 1H and 13C NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and chemical methods. The new structure of trisaccharide has been established: [Formula: see text] These trisaccharide O-antigen units substitute the core undecasaccharide at C-4 of the β-D-GlcpNAc residue. The core oligosaccharide and lipid A are identical with these of the serotype O17 (PCM 2231) (Maciejewska, A., Lukasiewicz, J., Kaszowska, M., Jachymek, W., Man-Kupisinska, A.; Lugowski, C. Mar. Drugs.2013, 11 (2), 440-454; Lukasiewicz, J., Dzieciatkowska, M., Niedziela, T., Jachymek, W., Augustyniuk, A., Kenne, L., Lugowski, C. Biochemistry, 2006, 45, 10434-10447).
-
the novel structure of the core oligosaccharide backbone of the lipopolysaccharide from the Plesiomonas shigelloides strain cnctc 80 89 serotype o13
Carbohydrate Research, 2013Co-Authors: Marta Kaszowska, Czeslaw Lugowski, Tomasz Niedziela, Wojciech Jachymek, Lennart Kenne, Sabina KojAbstract:The new structure of the core oligosaccharide of Plesiomonas shigelloides CNCTC 80/89 (serotype O13) lipopolysaccharide has been investigated by chemical methods, (1)H and (13)C NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight (MALDI-TOF). It was concluded that the core oligosaccharide of P. shigelloides CNCTC 80/89 is a nonasaccharide with the following structure: The position of glycine was determined by MALDI-TOF MS/MS analyses.
-
complete lipopolysaccharide of Plesiomonas shigelloides o74 h5 strain cnctc 144 92 1 structural analysis of the highly hydrophobic lipopolysaccharide including the o antigen its biological repeating unit the core oligosaccharide and the linkage betwe
Biochemistry, 2006Co-Authors: Tomasz Niedziela, Czeslaw Lugowski, Semiha Dag, Jolanta Lukasiewicz, Monika Dzieciatkowska, Wojciech Jachymek, Lennart KenneAbstract:The lipopolysaccharide of Plesiomonas shigelloides serotype O74:H5 (strain CNCTC 144/92) was obtained with the hot phenol/water method, but unlike most of the S-type enterobacterial lipopolysacchar...
-
structural studies of the o specific polysaccharide from Plesiomonas shigelloides strain cnctc 113 92
FEBS Journal, 2000Co-Authors: Jolanta Czaja, Czeslaw Lugowski, Tomasz Niedziela, Wojciech Jachymek, Eva Aldova, Lennart KenneAbstract:The structure of the O-specific side chain of the lipopolysaccharide (LPS) of Plesiomonas shigelloides, strain CNCTC 113/92 has been investigated by NMR spectroscopy, matrix-assisted laser desorption/ionization time of flight mass spectrometry and sugar and methylation analysis. It was concluded that the polysaccharide is composed of a hexasaccharide repeating unit with the following structure: in which D-beta-D-Hepp is Dglycero-beta-Dmanno-heptopyranose and 6d-beta-D-Hep is 6-deoxy-beta-Dmanno-heptopyranose. This structure represents a novel hexasaccharide repeating unit of bacterial O-antigen that is characteristic and unique to the Plesiomonas shigelloides strain. Using the high-resolution magic angle spinning technique, 1H-NMR spectra were also obtained for the O-polysaccharide components of isolated LPS and in their original form directly on the surface of bacterial cells.
Juan M Tomas - One of the best experts on this subject based on the ideXlab platform.
-
genomic and proteomic studies on Plesiomonas shigelloides lipopolysaccharide core biosynthesis
Journal of Bacteriology, 2014Co-Authors: Eleonora Aquilini, Susana Merino, Miguel Regue, Juan M TomasAbstract:We report here the identification of waa clusters with the genes required for the biosynthesis of the core lipopolysaccharides (LPS) of two Plesiomonas shigelloides strains. Both P. shigelloides waa clusters shared all of the genes besides the ones flanking waaL. In both strains, all of the genes were found in the waa gene cluster, although one common core biosynthetic gene (wapG) was found in a different chromosome location outside the cluster. Since P. shigelloides and Klebsiella pneumoniae share a core LPS carbohydrate backbone extending up at least to the second outer-core residue, the functions of the common P. shigelloides genes were elucidated by genetic complementation studies using well-defined K. pneumoniae mutants. The function of strain-specific inner- or outer-core genes was identified by using as a surrogate acceptor LPS from three well-defined K. pneumoniae core LPS mutants. Using this strategy, we were able to assign a proteomic function to all of the P. shigelloides waa genes identified in the two strains encoding six new glycosyltransferases (WapA, -B, -C, -D, -F, and -G). P. shigelloides demonstrated an important variety of core LPS structures, despite being a single species of the genus, as well as high homologous recombination in housekeeping genes.
-
genome sequence of Plesiomonas shigelloides strain 302 73 serotype o1
Genome Announcements, 2013Co-Authors: Nuria Pique, Eleonora Aquilini, Tyler Alioto, David Minanagalbis, Juan M TomasAbstract:ABSTRACT Plesiomonas shigelloides, the only species of the genus, is an emergent pathogenic bacterium associated with human diarrheal and extraintestinal disease. We present the whole-genome sequence analysis of the representative strain for the O1 serotype (strain 302-73), providing a tool for studying bacterial outbreaks, virulence factors, and accurate diagnostic methods.
-
structural studies of the o chain polysaccharide from Plesiomonas shigelloides strain 302 73 serotype o1
European Journal of Organic Chemistry, 2008Co-Authors: Giuseppina Pieretti, Maria Michela Corsaro, Rosa Lanzetta, Michelangelo Parrilli, Rocio Canals, Susana Merino, Juan M TomasAbstract:Plesiomonas shigelloides is a Gram-negative bacterium belonging to the Enterobacteriaceae family. It has been found in an aquatic environment in the tropical and subtropical regions and is responsible for many gastrointestinal infections in humans, which take place from drinking untreated water or eating uncooked shellfish. Plesiomonas shigelloides has also been reported to provoke extraintestinal infections such as meningitis and bacteremia in immunocompromised adults and neonates. Despite the emerging importance of this pathogenic microorganism, only three different O-antigens have been characterised so far. The structure of the O-chain of the lipopolysaccharide (LPS) from Plesiomonasshigelloides strain 302–73 (serotype O1) was determined by chemical analysis, 1D and 2D NMR spectroscopy and MALDI-TOF mass spectrometry. The polysaccharide was constituted by a linear pentasaccharidic repeating unit as follows: 3)-α-L-PneNAc4OAc(14)-α-L-FucNAc(14)-α-L-FucNAc(14)-α-L-FucNAc(13)-β-D-QuiNAc4NHb(1 (PneNAc = 2-acetamido-2,6-dideoxy-talose, Hb = (S)-3-hydroxybutanoyl) PneNAc O-acetylation was not stoichiometric and was found to be about 75 %. The position of the O-acetyl group and the amount of acetylation were deduced by NMR spectroscopic analysis. All the monosaccharides included in the repeating unit were deoxyamino sugars, which most probably, together with the presence of O-acetyl groups, were responsible for the recovery of the LPS in the phenol layer of the phenol/water extract of dried bacteria cells.(© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Doug Henderson - One of the best experts on this subject based on the ideXlab platform.
-
development of a method to produce hemoglobin in a bioreactor culture of escherichia coli bl21 de3 transformed with a plasmid containing Plesiomonas shigelloides heme transport genes and modified human hemoglobin genes
Applied and Environmental Microbiology, 2011Co-Authors: Bryan J Z Smith, P Gutierrez, E Guerrero, C J Brewer, Doug HendersonAbstract:We describe a method for production of recombinant human hemoglobin by Escherichia coli grown in a bioreactor. E. coli BL21(DE3) transformed with a plasmid containing hemoglobin genes and Plesiomonas shigelloides heme transport genes reached a cell dry weight of 83.64 g/liter and produced 11.92 g/liter of hemoglobin in clarified lysates.
-
Enhancement of Recombinant Hemoglobin Production in Escherichia coli BL21(DE3) Containing the Plesiomonas shigelloides Heme Transport System
Applied and Environmental Microbiology, 2008Co-Authors: D. M. Villarreal, John S. Olson, C. L. Phillips, A. M. Kelley, S. Villarreal, A. Villaloboz, P. Hernandez, Doug HendersonAbstract:To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) grown in medium containing heme.
Tomasz Niedziela - One of the best experts on this subject based on the ideXlab platform.
-
the o antigen of Plesiomonas shigelloides serotype o36 containing pseudaminic acid
Carbohydrate Research, 2016Co-Authors: Marta Kaszowska, Katarina Stojkovic, Tomasz Niedziela, Czeslaw LugowskiAbstract:Abstract The structure of the repeating unit of O-antigen of Plesiomonas shigelloides serotype O36 has been investigated by 1H and 13C NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and chemical methods. The new structure of trisaccharide has been established: →4)-β-Pse5Ac7(R3Hb)-(2 → 4)-β-D-Galp-(1 → 3)-β-D-GlcpNAc-(1→ These trisaccharide O-antigen units substitute the core undecasaccharide at C-4 of the β-D-GlcpNAc residue. The core oligosaccharide and lipid A are identical with these of the serotype O17 (PCM 2231) (Maciejewska, A., Lukasiewicz, J., Kaszowska, M., Jachymek, W., Man-Kupisinska, A.; Lugowski, C. Mar. Drugs. 2013, 11 (2), 440–454; Lukasiewicz, J., Dzieciatkowska, M., Niedziela, T., Jachymek, W., Augustyniuk, A., Kenne, L., Lugowski, C. Biochemistry, 2006, 45, 10434–10447).
-
the o antigen of Plesiomonas shigelloides serotype o36 containing pseudaminic acid
Carbohydrate Research, 2016Co-Authors: Marta Kaszowska, Katarina Stojkovic, Tomasz Niedziela, Czeslaw LugowskiAbstract:The structure of the repeating unit of O-antigen of Plesiomonas shigelloides serotype O36 has been investigated by 1H and 13C NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and chemical methods. The new structure of trisaccharide has been established: [Formula: see text] These trisaccharide O-antigen units substitute the core undecasaccharide at C-4 of the β-D-GlcpNAc residue. The core oligosaccharide and lipid A are identical with these of the serotype O17 (PCM 2231) (Maciejewska, A., Lukasiewicz, J., Kaszowska, M., Jachymek, W., Man-Kupisinska, A.; Lugowski, C. Mar. Drugs.2013, 11 (2), 440-454; Lukasiewicz, J., Dzieciatkowska, M., Niedziela, T., Jachymek, W., Augustyniuk, A., Kenne, L., Lugowski, C. Biochemistry, 2006, 45, 10434-10447).
-
the novel structure of the core oligosaccharide backbone of the lipopolysaccharide from the Plesiomonas shigelloides strain cnctc 80 89 serotype o13
Carbohydrate Research, 2013Co-Authors: Marta Kaszowska, Czeslaw Lugowski, Tomasz Niedziela, Wojciech Jachymek, Lennart Kenne, Sabina KojAbstract:The new structure of the core oligosaccharide of Plesiomonas shigelloides CNCTC 80/89 (serotype O13) lipopolysaccharide has been investigated by chemical methods, (1)H and (13)C NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight (MALDI-TOF). It was concluded that the core oligosaccharide of P. shigelloides CNCTC 80/89 is a nonasaccharide with the following structure: The position of glycine was determined by MALDI-TOF MS/MS analyses.
-
complete lipopolysaccharide of Plesiomonas shigelloides o74 h5 strain cnctc 144 92 1 structural analysis of the highly hydrophobic lipopolysaccharide including the o antigen its biological repeating unit the core oligosaccharide and the linkage betwe
Biochemistry, 2006Co-Authors: Tomasz Niedziela, Czeslaw Lugowski, Semiha Dag, Jolanta Lukasiewicz, Monika Dzieciatkowska, Wojciech Jachymek, Lennart KenneAbstract:The lipopolysaccharide of Plesiomonas shigelloides serotype O74:H5 (strain CNCTC 144/92) was obtained with the hot phenol/water method, but unlike most of the S-type enterobacterial lipopolysacchar...
-
structural studies of the o specific polysaccharide from Plesiomonas shigelloides strain cnctc 113 92
FEBS Journal, 2000Co-Authors: Jolanta Czaja, Czeslaw Lugowski, Tomasz Niedziela, Wojciech Jachymek, Eva Aldova, Lennart KenneAbstract:The structure of the O-specific side chain of the lipopolysaccharide (LPS) of Plesiomonas shigelloides, strain CNCTC 113/92 has been investigated by NMR spectroscopy, matrix-assisted laser desorption/ionization time of flight mass spectrometry and sugar and methylation analysis. It was concluded that the polysaccharide is composed of a hexasaccharide repeating unit with the following structure: in which D-beta-D-Hepp is Dglycero-beta-Dmanno-heptopyranose and 6d-beta-D-Hep is 6-deoxy-beta-Dmanno-heptopyranose. This structure represents a novel hexasaccharide repeating unit of bacterial O-antigen that is characteristic and unique to the Plesiomonas shigelloides strain. Using the high-resolution magic angle spinning technique, 1H-NMR spectra were also obtained for the O-polysaccharide components of isolated LPS and in their original form directly on the surface of bacterial cells.
Marta Kaszowska - One of the best experts on this subject based on the ideXlab platform.
-
the o antigen of Plesiomonas shigelloides serotype o36 containing pseudaminic acid
Carbohydrate Research, 2016Co-Authors: Marta Kaszowska, Katarina Stojkovic, Tomasz Niedziela, Czeslaw LugowskiAbstract:Abstract The structure of the repeating unit of O-antigen of Plesiomonas shigelloides serotype O36 has been investigated by 1H and 13C NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and chemical methods. The new structure of trisaccharide has been established: →4)-β-Pse5Ac7(R3Hb)-(2 → 4)-β-D-Galp-(1 → 3)-β-D-GlcpNAc-(1→ These trisaccharide O-antigen units substitute the core undecasaccharide at C-4 of the β-D-GlcpNAc residue. The core oligosaccharide and lipid A are identical with these of the serotype O17 (PCM 2231) (Maciejewska, A., Lukasiewicz, J., Kaszowska, M., Jachymek, W., Man-Kupisinska, A.; Lugowski, C. Mar. Drugs. 2013, 11 (2), 440–454; Lukasiewicz, J., Dzieciatkowska, M., Niedziela, T., Jachymek, W., Augustyniuk, A., Kenne, L., Lugowski, C. Biochemistry, 2006, 45, 10434–10447).
-
the o antigen of Plesiomonas shigelloides serotype o36 containing pseudaminic acid
Carbohydrate Research, 2016Co-Authors: Marta Kaszowska, Katarina Stojkovic, Tomasz Niedziela, Czeslaw LugowskiAbstract:The structure of the repeating unit of O-antigen of Plesiomonas shigelloides serotype O36 has been investigated by 1H and 13C NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and chemical methods. The new structure of trisaccharide has been established: [Formula: see text] These trisaccharide O-antigen units substitute the core undecasaccharide at C-4 of the β-D-GlcpNAc residue. The core oligosaccharide and lipid A are identical with these of the serotype O17 (PCM 2231) (Maciejewska, A., Lukasiewicz, J., Kaszowska, M., Jachymek, W., Man-Kupisinska, A.; Lugowski, C. Mar. Drugs.2013, 11 (2), 440-454; Lukasiewicz, J., Dzieciatkowska, M., Niedziela, T., Jachymek, W., Augustyniuk, A., Kenne, L., Lugowski, C. Biochemistry, 2006, 45, 10434-10447).
-
NMR Study of the O-Specific Polysaccharide and the Core Oligosaccharide from the Lipopolysaccharide Produced by Plesiomonas shigelloides O24:H8 (Strain CNCTC 92/89)
MDPI AG, 2015Co-Authors: Lena C. E. Lundqvist, Marta Kaszowska, Corine SandströmAbstract:The structures of the O-specific polysacccharide and core oligosaccharide of the lipopolysaccharide from Plesiomonas shigelloides O24:H8, strain CNCTC 92/89, have been investigated by NMR spectroscopy and ESI mass spectrometry. The O-specific polysaccharide was found to be composed of a tetrasaccharide repeating unit consisting of [→3)-α-FucpNAc-(1→3)-α-GalpNAcA-(1→3)-α-QuipNAc-(1→] and of α-RhapNAc (1→4) linked to the GalpNAcA residue. An identical structure has been reported for the capsular polysaccharide of the clinical isolate of Vibrio vulnificus strain BO62316 [1]. The core oligosaccharide was composed of a decasaccharide which structure is identical with these in P. shigelloides serotype O54 [2] and serotype O37 [3]
-
the novel structure of the core oligosaccharide backbone of the lipopolysaccharide from the Plesiomonas shigelloides strain cnctc 80 89 serotype o13
Carbohydrate Research, 2013Co-Authors: Marta Kaszowska, Czeslaw Lugowski, Tomasz Niedziela, Wojciech Jachymek, Lennart Kenne, Sabina KojAbstract:The new structure of the core oligosaccharide of Plesiomonas shigelloides CNCTC 80/89 (serotype O13) lipopolysaccharide has been investigated by chemical methods, (1)H and (13)C NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight (MALDI-TOF). It was concluded that the core oligosaccharide of P. shigelloides CNCTC 80/89 is a nonasaccharide with the following structure: The position of glycine was determined by MALDI-TOF MS/MS analyses.