The Experts below are selected from a list of 45303 Experts worldwide ranked by ideXlab platform
Carmen Saenz - One of the best experts on this subject based on the ideXlab platform.
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The SIN3A/HDAC Corepressor Complex Functionally Cooperates with NANOG to Promote Pluripotency
Cell reports, 2017Co-Authors: Arven Saunders, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Michael Reimer, Junjun Ding, Carlos Sanchez-priego, Carmen SaenzAbstract:Although SIN3A is required for the survival of early embryos and embryonic stem cells (ESCs), the role of SIN3A in the maintenance and establishment of Pluripotency remains unclear. Here, we find that the SIN3A/HDAC corepressor complex maintains ESC Pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the SIN3A/HDAC corepressor complex are enriched in an extended NANOG interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for SIN3A and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, NANOG and SIN3A co-occupy transcriptionally active Pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein Pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting Pluripotency.
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the sin3a hdac corepressor complex functionally cooperates with nanog to promote Pluripotency
Cell Reports, 2017Co-Authors: Arven Saunders, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Michael Reimer, Junjun Ding, Carlos Sanchezpriego, Carmen SaenzAbstract:Although SIN3A is required for the survival of early embryos and embryonic stem cells (ESCs), the role of SIN3A in the maintenance and establishment of Pluripotency remains unclear. Here, we find that the SIN3A/HDAC corepressor complex maintains ESC Pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the SIN3A/HDAC corepressor complex are enriched in an extended NANOG interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for SIN3A and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, NANOG and SIN3A co-occupy transcriptionally active Pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein Pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting Pluripotency.
Michael Reimer - One of the best experts on this subject based on the ideXlab platform.
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The SIN3A/HDAC Corepressor Complex Functionally Cooperates with NANOG to Promote Pluripotency
Cell reports, 2017Co-Authors: Arven Saunders, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Michael Reimer, Junjun Ding, Carlos Sanchez-priego, Carmen SaenzAbstract:Although SIN3A is required for the survival of early embryos and embryonic stem cells (ESCs), the role of SIN3A in the maintenance and establishment of Pluripotency remains unclear. Here, we find that the SIN3A/HDAC corepressor complex maintains ESC Pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the SIN3A/HDAC corepressor complex are enriched in an extended NANOG interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for SIN3A and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, NANOG and SIN3A co-occupy transcriptionally active Pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein Pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting Pluripotency.
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the sin3a hdac corepressor complex functionally cooperates with nanog to promote Pluripotency
Cell Reports, 2017Co-Authors: Arven Saunders, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Michael Reimer, Junjun Ding, Carlos Sanchezpriego, Carmen SaenzAbstract:Although SIN3A is required for the survival of early embryos and embryonic stem cells (ESCs), the role of SIN3A in the maintenance and establishment of Pluripotency remains unclear. Here, we find that the SIN3A/HDAC corepressor complex maintains ESC Pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the SIN3A/HDAC corepressor complex are enriched in an extended NANOG interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for SIN3A and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, NANOG and SIN3A co-occupy transcriptionally active Pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein Pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting Pluripotency.
Arven Saunders - One of the best experts on this subject based on the ideXlab platform.
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The SIN3A/HDAC Corepressor Complex Functionally Cooperates with NANOG to Promote Pluripotency
Cell reports, 2017Co-Authors: Arven Saunders, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Michael Reimer, Junjun Ding, Carlos Sanchez-priego, Carmen SaenzAbstract:Although SIN3A is required for the survival of early embryos and embryonic stem cells (ESCs), the role of SIN3A in the maintenance and establishment of Pluripotency remains unclear. Here, we find that the SIN3A/HDAC corepressor complex maintains ESC Pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the SIN3A/HDAC corepressor complex are enriched in an extended NANOG interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for SIN3A and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, NANOG and SIN3A co-occupy transcriptionally active Pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein Pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting Pluripotency.
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the sin3a hdac corepressor complex functionally cooperates with nanog to promote Pluripotency
Cell Reports, 2017Co-Authors: Arven Saunders, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Michael Reimer, Junjun Ding, Carlos Sanchezpriego, Carmen SaenzAbstract:Although SIN3A is required for the survival of early embryos and embryonic stem cells (ESCs), the role of SIN3A in the maintenance and establishment of Pluripotency remains unclear. Here, we find that the SIN3A/HDAC corepressor complex maintains ESC Pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the SIN3A/HDAC corepressor complex are enriched in an extended NANOG interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for SIN3A and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, NANOG and SIN3A co-occupy transcriptionally active Pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein Pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting Pluripotency.
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tex10 coordinates epigenetic control of super enhancer activity in Pluripotency and reprogramming
Cell Stem Cell, 2015Co-Authors: Junjun Ding, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Arven Saunders, Ningyi Shao, Hongwei Zhou, Dung Fang Lee, Pavel V ShliahaAbstract:Super-enhancers (SEs) are large clusters of transcriptional enhancers that are co-occupied by multiple lineage-specific transcription factors driving expression of genes that define cell identity. In embryonic stem cells (ESCs), SEs are highly enriched for the core Pluripotency factors Oct4, Sox2, and Nanog. In this study, we sought to dissect the molecular control mechanism of SE activity in Pluripotency and reprogramming. Starting from a protein interaction network surrounding Sox2, we identified Tex10 as a key Pluripotency factor that plays a functionally significant role in ESC self-renewal, early embryo development, and reprogramming. Tex10 is enriched at SEs in a Sox2-dependent manner and coordinates histone acetylation and DNA demethylation at SEs. Tex10 activity is also important for Pluripotency and reprogramming in human cells. Our study therefore highlights Tex10 as a core component of the Pluripotency network and sheds light on its role in epigenetic control of SE activity for cell fate determination.
Xin Huang - One of the best experts on this subject based on the ideXlab platform.
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yy1 positively regulates transcription by targeting promoters and super enhancers through the baf complex in embryonic stem cells
Stem cell reports, 2018Co-Authors: Jia Wang, Chao Wei, Xin Huang, Xiaona Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Tingyuan Huang, Di Peng, Li ChenAbstract:Yin Yang 1 (YY1) regulates early embryogenesis and adult tissue formation. However, the role of YY1 in stem cell regulation remains unclear. YY1 has a Polycomb group (PcG) protein-dependent role in mammalian cells. The PcG-independent functions of YY1 are also reported, although their underlying mechanism is still undefined. This paper reports the role of YY1 and BAF complex in the OCT4-mediated Pluripotency network in mouse embryonic stem cells (mESCs). The interaction between YY1 and BAF complex promotes mESC proliferation and Pluripotency. Knockdown of Yy1 or Smarca4, the core component of the BAF complex, downregulates Pluripotency markers and upregulates several differentiation markers. Moreover, YY1 enriches at both promoter and super-enhancer regions to stimulate transcription. Thus, this study elucidates the role of YY1 in regulating Pluripotency through its interaction with OCT4 and the BAF complex and the role of BAF complex in integrating YY1 into the core Pluripotency network.
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The SIN3A/HDAC Corepressor Complex Functionally Cooperates with NANOG to Promote Pluripotency
Cell reports, 2017Co-Authors: Arven Saunders, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Michael Reimer, Junjun Ding, Carlos Sanchez-priego, Carmen SaenzAbstract:Although SIN3A is required for the survival of early embryos and embryonic stem cells (ESCs), the role of SIN3A in the maintenance and establishment of Pluripotency remains unclear. Here, we find that the SIN3A/HDAC corepressor complex maintains ESC Pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the SIN3A/HDAC corepressor complex are enriched in an extended NANOG interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for SIN3A and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, NANOG and SIN3A co-occupy transcriptionally active Pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein Pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting Pluripotency.
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the sin3a hdac corepressor complex functionally cooperates with nanog to promote Pluripotency
Cell Reports, 2017Co-Authors: Arven Saunders, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Michael Reimer, Junjun Ding, Carlos Sanchezpriego, Carmen SaenzAbstract:Although SIN3A is required for the survival of early embryos and embryonic stem cells (ESCs), the role of SIN3A in the maintenance and establishment of Pluripotency remains unclear. Here, we find that the SIN3A/HDAC corepressor complex maintains ESC Pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the SIN3A/HDAC corepressor complex are enriched in an extended NANOG interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for SIN3A and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, NANOG and SIN3A co-occupy transcriptionally active Pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein Pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting Pluripotency.
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tex10 coordinates epigenetic control of super enhancer activity in Pluripotency and reprogramming
Cell Stem Cell, 2015Co-Authors: Junjun Ding, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Arven Saunders, Ningyi Shao, Hongwei Zhou, Dung Fang Lee, Pavel V ShliahaAbstract:Super-enhancers (SEs) are large clusters of transcriptional enhancers that are co-occupied by multiple lineage-specific transcription factors driving expression of genes that define cell identity. In embryonic stem cells (ESCs), SEs are highly enriched for the core Pluripotency factors Oct4, Sox2, and Nanog. In this study, we sought to dissect the molecular control mechanism of SE activity in Pluripotency and reprogramming. Starting from a protein interaction network surrounding Sox2, we identified Tex10 as a key Pluripotency factor that plays a functionally significant role in ESC self-renewal, early embryo development, and reprogramming. Tex10 is enriched at SEs in a Sox2-dependent manner and coordinates histone acetylation and DNA demethylation at SEs. Tex10 activity is also important for Pluripotency and reprogramming in human cells. Our study therefore highlights Tex10 as a core component of the Pluripotency network and sheds light on its role in epigenetic control of SE activity for cell fate determination.
Miguel Fidalgo - One of the best experts on this subject based on the ideXlab platform.
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yy1 positively regulates transcription by targeting promoters and super enhancers through the baf complex in embryonic stem cells
Stem cell reports, 2018Co-Authors: Jia Wang, Chao Wei, Xin Huang, Xiaona Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Tingyuan Huang, Di Peng, Li ChenAbstract:Yin Yang 1 (YY1) regulates early embryogenesis and adult tissue formation. However, the role of YY1 in stem cell regulation remains unclear. YY1 has a Polycomb group (PcG) protein-dependent role in mammalian cells. The PcG-independent functions of YY1 are also reported, although their underlying mechanism is still undefined. This paper reports the role of YY1 and BAF complex in the OCT4-mediated Pluripotency network in mouse embryonic stem cells (mESCs). The interaction between YY1 and BAF complex promotes mESC proliferation and Pluripotency. Knockdown of Yy1 or Smarca4, the core component of the BAF complex, downregulates Pluripotency markers and upregulates several differentiation markers. Moreover, YY1 enriches at both promoter and super-enhancer regions to stimulate transcription. Thus, this study elucidates the role of YY1 in regulating Pluripotency through its interaction with OCT4 and the BAF complex and the role of BAF complex in integrating YY1 into the core Pluripotency network.
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The SIN3A/HDAC Corepressor Complex Functionally Cooperates with NANOG to Promote Pluripotency
Cell reports, 2017Co-Authors: Arven Saunders, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Michael Reimer, Junjun Ding, Carlos Sanchez-priego, Carmen SaenzAbstract:Although SIN3A is required for the survival of early embryos and embryonic stem cells (ESCs), the role of SIN3A in the maintenance and establishment of Pluripotency remains unclear. Here, we find that the SIN3A/HDAC corepressor complex maintains ESC Pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the SIN3A/HDAC corepressor complex are enriched in an extended NANOG interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for SIN3A and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, NANOG and SIN3A co-occupy transcriptionally active Pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein Pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting Pluripotency.
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the sin3a hdac corepressor complex functionally cooperates with nanog to promote Pluripotency
Cell Reports, 2017Co-Authors: Arven Saunders, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Michael Reimer, Junjun Ding, Carlos Sanchezpriego, Carmen SaenzAbstract:Although SIN3A is required for the survival of early embryos and embryonic stem cells (ESCs), the role of SIN3A in the maintenance and establishment of Pluripotency remains unclear. Here, we find that the SIN3A/HDAC corepressor complex maintains ESC Pluripotency and promotes the generation of induced pluripotent stem cells (iPSCs). Members of the SIN3A/HDAC corepressor complex are enriched in an extended NANOG interactome and function in transcriptional coactivation in ESCs. We also identified a critical role for SIN3A and HDAC2 in efficient reprogramming of somatic cells. Mechanistically, NANOG and SIN3A co-occupy transcriptionally active Pluripotency genes in ESCs and also co-localize extensively at their genome-wide targets in pre-iPSCs. Additionally, both factors are required to directly induce a synergistic transcriptional program wherein Pluripotency genes are activated and reprogramming barrier genes are repressed. Our findings indicate a transcriptional regulatory role for a major HDAC-containing complex in promoting Pluripotency.
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tex10 coordinates epigenetic control of super enhancer activity in Pluripotency and reprogramming
Cell Stem Cell, 2015Co-Authors: Junjun Ding, Xin Huang, Francesco Faiola, Diana Guallar, Miguel Fidalgo, Arven Saunders, Ningyi Shao, Hongwei Zhou, Dung Fang Lee, Pavel V ShliahaAbstract:Super-enhancers (SEs) are large clusters of transcriptional enhancers that are co-occupied by multiple lineage-specific transcription factors driving expression of genes that define cell identity. In embryonic stem cells (ESCs), SEs are highly enriched for the core Pluripotency factors Oct4, Sox2, and Nanog. In this study, we sought to dissect the molecular control mechanism of SE activity in Pluripotency and reprogramming. Starting from a protein interaction network surrounding Sox2, we identified Tex10 as a key Pluripotency factor that plays a functionally significant role in ESC self-renewal, early embryo development, and reprogramming. Tex10 is enriched at SEs in a Sox2-dependent manner and coordinates histone acetylation and DNA demethylation at SEs. Tex10 activity is also important for Pluripotency and reprogramming in human cells. Our study therefore highlights Tex10 as a core component of the Pluripotency network and sheds light on its role in epigenetic control of SE activity for cell fate determination.
