The Experts below are selected from a list of 4068 Experts worldwide ranked by ideXlab platform
Siegfried Ansorge - One of the best experts on this subject based on the ideXlab platform.
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dipeptidyl peptidase iv cd26 on human lymphocytes synthetic inhibitors of and antibodies against dipeptidyl peptidase iv suppress the proliferation of Pokeweed Mitogen stimulated peripheral blood mononuclear cells and il 2 and il 6 production
Immunobiology, 1993Co-Authors: Dirk Reinhold, H D Flad, Ute Bank, Frank Buhling, Klaus Neubert, Taila Mattern, Artur J Ulmer, Siegfried AnsorgeAbstract:Abstract In the present report, we describe that synthetic inhibitors of and polyclonal and monoclonal antibodies against the membrane ectoenzyme dipeptidyl peptidase IV (DP IV, CD26) inhibit the production of IL-2 and IL-6 and, concomitantly, DNA synthesis of Pokeweed Mitogen-stimulated peripheral blood mononuclear cells (PBMC). The release of IL-1 and TNF-α, was not influenced under these conditions. The data support the hypothesis that DP IV, possibly in conjunction with other peptidases, is involved in the regulation of activation and proliferation of T lymphocytes.
H D Flad - One of the best experts on this subject based on the ideXlab platform.
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dipeptidyl peptidase iv cd26 on human lymphocytes synthetic inhibitors of and antibodies against dipeptidyl peptidase iv suppress the proliferation of Pokeweed Mitogen stimulated peripheral blood mononuclear cells and il 2 and il 6 production
Immunobiology, 1993Co-Authors: Dirk Reinhold, H D Flad, Ute Bank, Frank Buhling, Klaus Neubert, Taila Mattern, Artur J Ulmer, Siegfried AnsorgeAbstract:Abstract In the present report, we describe that synthetic inhibitors of and polyclonal and monoclonal antibodies against the membrane ectoenzyme dipeptidyl peptidase IV (DP IV, CD26) inhibit the production of IL-2 and IL-6 and, concomitantly, DNA synthesis of Pokeweed Mitogen-stimulated peripheral blood mononuclear cells (PBMC). The release of IL-1 and TNF-α, was not influenced under these conditions. The data support the hypothesis that DP IV, possibly in conjunction with other peptidases, is involved in the regulation of activation and proliferation of T lymphocytes.
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fcr and fcr monocytes differentially secrete monokines during Pokeweed Mitogen induced t cell monocyte interactions
Immunology, 1992Co-Authors: Juliusz Pryjma, Marek Zembala, M Ernst, Bozenna Mytar, H Loppnow, H D FladAbstract:Monocyte subpopulations which differ in the expression of Fc receptor for human IgG (FcRI) differentially regulate the T-cell-dependent, Pokeweed Mitogen (PWM)-induced, polyclonal B-cell response. We, thus, studied the cytokine production in human peripheral blood monocyte and T-lymphocyte cultures activated with this lectin. Monocytes or their FcR+ and FcR- subpopulations stimulated with PWM were cultured with or without T lymphocytes or their CD4+ and CD8+ subsets. Both monocyte subpopulations cultured alone produced similar amounts of tumour necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), but FcR- monocytes showed significantly enhanced ability to secrete interleukin-1 (IL-1). T cells, especially CD4+, added to monocyte cultures enhanced IL-1 production. This enhancement was presumably due to interferon-gamma (IFN-gamma) release by T lymphocytes, since this lymphokine enhanced IL-1 secretion when added to PWM-stimulated cultures of monocytes. Addition of monocytes, in particular the FcR+ subpopulation, greatly enhanced production of IFN-gamma by T lymphocytes. Although both T-cell subsets produced IFN-gamma, the CD4+ cells were more efficient. These results indicate that in PWM-stimulated cultures subpopulations of monocytes differ in secretion of cytokines, which might explain their differential effect on T-cell-dependent immune responses in vitro.
Monika Lindemann - One of the best experts on this subject based on the ideXlab platform.
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Early suppression of peripheral mononuclear blood cells in sepsis in response to stimulation with cytomegalovirus, OKT3, and Pokeweed Mitogen.
Journal of Applied Physiology, 2019Co-Authors: Nathalie Maria Malewicz, Kai Walstein, Torsten Heine, Andrea Engler, Alexandra Bick, Annika Dötsch, Astrid M Westendorf, Peter A Horn, Monika LindemannAbstract:We observed suppression of reactivity to stimulation with cytomegalovirus, muromonab-CD3, and Pokeweed Mitogen in mononuclear blood cells of patients with early sepsis when compared with postoperat...
Dirk Reinhold - One of the best experts on this subject based on the ideXlab platform.
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dipeptidyl peptidase iv cd26 on human lymphocytes synthetic inhibitors of and antibodies against dipeptidyl peptidase iv suppress the proliferation of Pokeweed Mitogen stimulated peripheral blood mononuclear cells and il 2 and il 6 production
Immunobiology, 1993Co-Authors: Dirk Reinhold, H D Flad, Ute Bank, Frank Buhling, Klaus Neubert, Taila Mattern, Artur J Ulmer, Siegfried AnsorgeAbstract:Abstract In the present report, we describe that synthetic inhibitors of and polyclonal and monoclonal antibodies against the membrane ectoenzyme dipeptidyl peptidase IV (DP IV, CD26) inhibit the production of IL-2 and IL-6 and, concomitantly, DNA synthesis of Pokeweed Mitogen-stimulated peripheral blood mononuclear cells (PBMC). The release of IL-1 and TNF-α, was not influenced under these conditions. The data support the hypothesis that DP IV, possibly in conjunction with other peptidases, is involved in the regulation of activation and proliferation of T lymphocytes.
Wassim Y Almawi - One of the best experts on this subject based on the ideXlab platform.
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suppression of Pokeweed Mitogen driven human igm and igg responses by the hydroxylamine of sulfamethoxazole
International Journal of Immunopharmacology, 1997Co-Authors: Erin M Sisson, Michael J Rieder, Ingrid A Bird, Wassim Y AlmawiAbstract:OBJECTIVE To determine the effect(s) of reactive sulfonamide metabolites on antibody production by human lymphocytes. METHODS Human peripheral blood cells (PBMCs) were isolated from control volunteers and incubated with the hydroxylamine of sulfamethoxazole (SMX H/A), a reactive metabolite of the most commonly used sulfonamide, in increasing concentrations. PBMCs were then stimulated to produce antibody with Pokeweed Mitogen. After incubation for 8 days, concentrations of IgG and IgM were determined in supernatant using an ELISA assay. RESULTS Production of both IgG and IgM was significantly suppressed by sub-lethal concentrations of SMX H/A in a concentration-dependent fashion (p < 0.05). Suppression was more marked for IgM production (maximal decline to 80% of baseline antibody production) than for IgG production (maximal decline to 57% of baseline antibody production). No suppression was seen when cells were incubated with sulfamethoxazole in concentrations up to 400 microM. This suppression was not related to changes in cell viability; at a concentration of 25 microM of SMX H/A, IgM and IgG concentration were reduced by 47 +/- 8.7% and 73 +/- 7.2%, while cell viability (percentage of live cells) was 93 +/- 5%. Suppression was time-dependent, increasing over the incubation periods to reach a plateau after 2 h of incubation. CONCLUSION Sulfonamide reactive metabolites, in concentrations which are achieved during therapy, suppress antibody production by PWM-stimulated human cells. This may explain, in part, the alterations in immunity associated with hypersensitivity reactions to the sulfonamides. This may also have implications for patients receiving sulfonamide therapy and concurrent immunosuppressive therapy.