The Experts below are selected from a list of 956886 Experts worldwide ranked by ideXlab platform
Pankaj Garg - One of the best experts on this subject based on the ideXlab platform.
-
Comparison of histopathology and real-time Polymerase Chain Reaction (RT-PCR) for detection of Mycobacterium tuberculosis in fistula-in-ano
International Journal of Colorectal Disease, 2017Co-Authors: Pankaj GargAbstract:Purpose Histopathology is commonly used to diagnose tuberculosis in fistula-in-ano. The aim was to compare the sensitivity of Polymerase Chain Reaction and histopathology in detecting tuberculosis in fistula-in-ano. Methods The histopathology and Polymerase Chain-Reaction of tissue (fistula tract) was done in all the consecutive operated cases. When pus sample was also available, Polymerase Chain Reaction-pus was also done Results Three hundred forty seven samples (179 patients) were tested over 2 years (median 6.5 months). The mean age was 38.8 ± 10.7 years, and male/female was 170/9. Histopathology and Polymerase Chain Reaction of tissue (fistula tract) was done in 152 and 165 patients, respectively. Polymerase Chain Reaction (pus) could be done in 30 patients. Overall, tuberculosis was detected in 20/179 (11.2%) patients. Of these, tuberculosis was detected by histopathology (tissue) in 1/152 (0.7%) and by Polymerase Chain Reaction (tissue) in 14/165 (8.5%) patients. In pus, Polymerase Chain Reaction detected tuberculosis in 6/30 (20%) patients. Both Polymerase Chain Reaction of tissue and pus were positive in one patient. Polymerase Chain Reaction (tissue) and Polymerase Chain Reaction (pus) were significantly more sensitive than histopathology (tissue) for detecting tuberculosis [histopathology 1/152 vs. Polymerase Chain Reaction (tissue) 14/165, p = 0.0009] [histopathology 1/152 vs. Polymerase Chain Reaction (pus) 6/30, p
Shigeaki Ohno - One of the best experts on this subject based on the ideXlab platform.
-
detection of enterovirus 70 by Polymerase Chain Reaction in acute hemorrhagic conjunctivitis
American Journal of Ophthalmology, 1996Co-Authors: Eiichi Uchio, Koki Aoki, Kenji Yamazaki, Shigeaki OhnoAbstract:Purpose We have developed and applied reverse transcription-Polymerase Chain Reaction for the detection of enterovirus 70 from conjunctival swabs of patients among the population of an acute hemorrhagic conjunctivitis epidemic in Okinawa, Japan, in 1994. Methods The primers used were selected from the VP1, VP2, and VP3 regions of the enterovirus 70 nucleotide sequence. Results Twelve of 27 culture-negative specimens were positive by reverse transcription-Polymerase Chain Reaction. Conclusions The reverse transcription-Polymerase Chain Reaction test provides a reliable diagnostic method that readily allows specific detection of enterovirus 70 in patients with acute hemorrhagic conjunctivitis in whom low enterovirus 70 recovery rates have been obtained recently by cell culture.
Mei-ying Chuang - One of the best experts on this subject based on the ideXlab platform.
-
Application of Real-time Polymerase Chain Reaction (RT-PCR)
2006Co-Authors: Kuan-jiunn Shieh, Geroge Chen, X. Tracy Qiao, Mei-ying ChuangAbstract:The real-time Polymerase Chain Reaction (RT-PCR), also called quantitative real-time Polymerase Chain Reaction (QRT-PCR) or kinetic Polymerase Chain Reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule. It is used to determine whether a specific DNA sequence is present in the sample; and if it is present, the number of copies in the sample. It is the real-time version of quantitative Polymerase Chain Reaction (qPCR), itself a modification of Polymerase Chain Reaction (PCR). The procedure of RT-PCR follows the regular PCR procedure, but the DNA is quantified after each round of amplification. Two common methods of quantification are the use of fluorescent dyes that intercalate with double-strand DNA, and modified DNA oligonucleotide probes that fluoresce when hybridized with a complementary DNA. RT-PCR could be combined with reverse transcription Polymerase Chain Reaction to quantify messenger RNA (mRNA) at a particular time for in a particular cell or tissue type. (The Journal of American Science. 2006;2(3):1-15).
Eiichi Uchio - One of the best experts on this subject based on the ideXlab platform.
-
detection of enterovirus 70 by Polymerase Chain Reaction in acute hemorrhagic conjunctivitis
American Journal of Ophthalmology, 1996Co-Authors: Eiichi Uchio, Koki Aoki, Kenji Yamazaki, Shigeaki OhnoAbstract:Purpose We have developed and applied reverse transcription-Polymerase Chain Reaction for the detection of enterovirus 70 from conjunctival swabs of patients among the population of an acute hemorrhagic conjunctivitis epidemic in Okinawa, Japan, in 1994. Methods The primers used were selected from the VP1, VP2, and VP3 regions of the enterovirus 70 nucleotide sequence. Results Twelve of 27 culture-negative specimens were positive by reverse transcription-Polymerase Chain Reaction. Conclusions The reverse transcription-Polymerase Chain Reaction test provides a reliable diagnostic method that readily allows specific detection of enterovirus 70 in patients with acute hemorrhagic conjunctivitis in whom low enterovirus 70 recovery rates have been obtained recently by cell culture.
W. G. Quint - One of the best experts on this subject based on the ideXlab platform.
-
Detection ofChlamydia trachomatis in clinical specimens by the Polymerase Chain Reaction
European Journal of Clinical Microbiology and Infectious Diseases, 1990Co-Authors: H. C. Claas, W. J. Melchers, I. H. Bruijn, M. Graaf, W. C. Dijk, J. Lindeman, W. G. QuintAbstract:Sequences derived from the endogenous plasmid of Chlamydia trachomatis and from the genes coding for ribosomal 16S RNA of Chlamydia psittaci were used as primers and oligonucleotide probes for detection of chlamydiae by the Polymerase Chain Reaction. The endogenous plasmid primers generated specific amplified products of 517 bp with all known Chlamydia trachomatis serovars. No specific products of Chlamydia psittaci and Chlamydia pneumoniae could be detected using these primers. With the rRNA primers specific amplified products of 208 bp were generated with Chlamydia psittaci, Chlamydia trachomatis and Chlamydia pneumoniae . No specific amplified products were detected with DNA isolated from a variety of microorganisms from the urogenital and the respiratory tract. Of 156 clinical specimens used for evaluation of the Polymerase Chain Reaction, 26 were found to be positive for Chlamydia trachomatis on culture. All 26 culture positive samples were also found to be positive for Chalmydia trachomatis DNA by the Polymerase Chain Reaction with both primer sets. Two culture negative samples were also found to be positive by this technique. The Polymerase Chain Reaction thus seems to be a sensitive and reliable method for detection of Chlamydia trachomatis .
-
Detection of Chlamydia trachomatis in clinical specimens by the Polymerase Chain Reaction.
European Journal of Clinical Microbiology & Infectious Diseases, 1990Co-Authors: H. C. Claas, W. J. Melchers, I. H. Bruijn, M. Graaf, J. Lindeman, W. C. Van Dijk, W. G. QuintAbstract:Sequences derived from the endogenous plasmid ofChlamydia trachomatis and from the genes coding for ribosomal 16S RNA ofChlamydia psittaci were used as primers and oligonucleotide probes for detection of chlamydiae by the Polymerase Chain Reaction. The endogenous plasmid primers generated specific amplified products of 517 bp with all knownChlamydia trachomatis serovars. No specific products ofChlamydia psittaci andChlamydia pneumoniae could be detected using these primers. With the rRNA primers specific amplified products of 208 bp were generated withChlamydia psittaci, Chlamydia trachomatis andChlamydia pneumoniae. No specific amplified products were detected with DNA isolated from a variety of microorganisms from the urogenital and the respiratory tract. Of 156 clinical specimens used for evaluation of the Polymerase Chain Reaction, 26 were found to be positive forChlamydia trachomatis on culture. All 26 culture positive samples were also found to be positive forChalmydia trachomatis DNA by the Polymerase Chain Reaction with both primer sets. Two culture negative samples were also found to be positive by this technique. The Polymerase Chain Reaction thus seems to be a sensitive and reliable method for detection ofChlamydia trachomatis.