The Experts below are selected from a list of 798 Experts worldwide ranked by ideXlab platform
Ana Aguedapinto - One of the best experts on this subject based on the ideXlab platform.
-
coinfections of novel polyomavirus anelloviruses and a recombinant strain of myxoma virus myxv tol identified in iberian hares
Viruses, 2020Co-Authors: Ana Aguedapinto, Simona Kraberger, Michael C Lund, Christian Gortazar, Grant Mcfadden, Arvind Varsani, Pedro J EstevesAbstract:Viruses are ubiquitous in nature; however, very few have been identified in the Leporid species. In the fall of 2018, an outbreak of myxomatosis in Iberian hares (Lepus granatensis) was reported in Spain and a novel recombinant myxoma virus strain (MYXV-Tol) was identified. To investigate variability within the recombinant region of the MYXV-Tol and identify any potential viral coinfections, samples (ear, eyelid or vaginal) of Iberian hares were collected from Spain and analyzed. The presence of the recombinant region of the MYXV-Tol was confirmed in six out of eleven samples analyzed. Additionally, a polyomavirus (family Polyomaviridae), representing a putative new species, and anelloviruses (family Anelloviridae) belonging to two putative species were identified, some as coinfection with the recombinant MYXV-Tol. The two polyomavirus genomes were identified in two hares and share >99% genome-wide identity. Based on the analysis of their large T-antigen, the new polyomavirus clusters in a distant clade from other mammals sharing <64% amino acid identity. A total of 14 anelloviruses were identified, which share 63-99% genome-wide identity. Overall, our results show a coinfection of different DNA viruses in the studied samples and raise awareness regarding the extensive unsampled diversity of viruses in hares.
David Wang - One of the best experts on this subject based on the ideXlab platform.
-
Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections
2013Co-Authors: Anne M. Gaynor, Daniel C Brennan, Michael D. Nissen, David M. Whiley, Ian M. Mackay, Stephen B. Lambert, Gregory A. Storch, Theo P. Sloots, David WangAbstract:We report the identification of a novel polyomavirus present in respiratory secretions from human patients with symptoms of acute respiratory tract infection. The virus was initially detected in a nasopharyngeal aspirate from a 3-year-old child from Australia diagnosed with pneumonia. A random library was generated from nucleic acids extracted from the nasopharyngeal aspirate and analyzed by high throughput DNA sequencing. Multiple DNA fragments were cloned that possessed limited homology to known polyomaviruses. We subsequently sequenced the entire virus genome of 5,229 bp, henceforth referred to as WU virus, and found it to have genomic features characteristic of the family Polyomaviridae. The genome was predicted to encode small T antigen, large T antigen, and three capsid proteins: VP1, VP2, and VP3. Phylogenetic analysis clearly revealed that the WU virus was divergent from all known polyomaviruses. Screening of 2,135 patients with acute respiratory tract infections in Brisbane, Queensland, Australia
-
identification of mw polyomavirus a novel polyomavirus in human stool
Journal of Virology, 2012Co-Authors: Erica A Siebrasse, Alejandro Reyes, Rajhab S Mkakosya, Mark J Manary, Guoyan Zhao, Jeffrey I Gordon, David WangAbstract:We have discovered a novel polyomavirus present in multiple human stool samples. The virus was initially identified by shotgun pyrosequencing of DNA purified from virus-like particles isolated from a stool sample collected from a healthy child from Malawi. We subsequently sequenced the virus' 4,927-bp genome, which has been provisionally named MW polyomavirus (MWPyV). The virus has genomic features characteristic of the family Polyomaviridae but is highly divergent from other members of this family. It is predicted to encode the large T antigen and small T antigen early proteins and the VP1, VP2, and VP3 structural proteins. A real-time PCR assay was designed and used to screen 514 stool samples from children with diarrhea in St. Louis, MO; 12 specimens were positive for MWPyV. Comparison of the whole-genome sequences of the index Malawi case and one St. Louis case demonstrated that the two strains of MWPyV varied by 5.3% at the nucleotide level. The number of polyomaviruses found in the human body continues to grow, raising the question of how many more species have yet to be identified and what roles they play in humans with and without manifest disease.
-
identification of a novel polyomavirus from patients with acute respiratory tract infections
PLOS Pathogens, 2007Co-Authors: Anne M. Gaynor, Daniel C Brennan, Michael D. Nissen, David M. Whiley, Ian M. Mackay, Stephen B. Lambert, Gregory A. Storch, Theo P. Sloots, Guang Wu, David WangAbstract:We report the identification of a novel polyomavirus present in respiratory secretions from human patients with symptoms of acute respiratory tract infection. The virus was initially detected in a nasopharyngeal aspirate from a 3-year-old child from Australia diagnosed with pneumonia. A random library was generated from nucleic acids extracted from the nasopharyngeal aspirate and analyzed by high throughput DNA sequencing. Multiple DNA fragments were cloned that possessed limited homology to known polyomaviruses. We subsequently sequenced the entire virus genome of 5,229 bp, henceforth referred to as WU virus, and found it to have genomic features characteristic of the family Polyomaviridae. The genome was predicted to encode small T antigen, large T antigen, and three capsid proteins: VP1, VP2, and VP3. Phylogenetic analysis clearly revealed that the WU virus was divergent from all known polyomaviruses. Screening of 2,135 patients with acute respiratory tract infections in Brisbane, Queensland, Australia, and St. Louis, Missouri, United States, using WU virus–specific PCR primers resulted in the detection of 43 additional specimens that contained WU virus. The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen.
Pedro J Esteves - One of the best experts on this subject based on the ideXlab platform.
-
coinfections of novel polyomavirus anelloviruses and a recombinant strain of myxoma virus myxv tol identified in iberian hares
Viruses, 2020Co-Authors: Ana Aguedapinto, Simona Kraberger, Michael C Lund, Christian Gortazar, Grant Mcfadden, Arvind Varsani, Pedro J EstevesAbstract:Viruses are ubiquitous in nature; however, very few have been identified in the Leporid species. In the fall of 2018, an outbreak of myxomatosis in Iberian hares (Lepus granatensis) was reported in Spain and a novel recombinant myxoma virus strain (MYXV-Tol) was identified. To investigate variability within the recombinant region of the MYXV-Tol and identify any potential viral coinfections, samples (ear, eyelid or vaginal) of Iberian hares were collected from Spain and analyzed. The presence of the recombinant region of the MYXV-Tol was confirmed in six out of eleven samples analyzed. Additionally, a polyomavirus (family Polyomaviridae), representing a putative new species, and anelloviruses (family Anelloviridae) belonging to two putative species were identified, some as coinfection with the recombinant MYXV-Tol. The two polyomavirus genomes were identified in two hares and share >99% genome-wide identity. Based on the analysis of their large T-antigen, the new polyomavirus clusters in a distant clade from other mammals sharing <64% amino acid identity. A total of 14 anelloviruses were identified, which share 63-99% genome-wide identity. Overall, our results show a coinfection of different DNA viruses in the studied samples and raise awareness regarding the extensive unsampled diversity of viruses in hares.
Arvind Varsani - One of the best experts on this subject based on the ideXlab platform.
-
coinfections of novel polyomavirus anelloviruses and a recombinant strain of myxoma virus myxv tol identified in iberian hares
Viruses, 2020Co-Authors: Ana Aguedapinto, Simona Kraberger, Michael C Lund, Christian Gortazar, Grant Mcfadden, Arvind Varsani, Pedro J EstevesAbstract:Viruses are ubiquitous in nature; however, very few have been identified in the Leporid species. In the fall of 2018, an outbreak of myxomatosis in Iberian hares (Lepus granatensis) was reported in Spain and a novel recombinant myxoma virus strain (MYXV-Tol) was identified. To investigate variability within the recombinant region of the MYXV-Tol and identify any potential viral coinfections, samples (ear, eyelid or vaginal) of Iberian hares were collected from Spain and analyzed. The presence of the recombinant region of the MYXV-Tol was confirmed in six out of eleven samples analyzed. Additionally, a polyomavirus (family Polyomaviridae), representing a putative new species, and anelloviruses (family Anelloviridae) belonging to two putative species were identified, some as coinfection with the recombinant MYXV-Tol. The two polyomavirus genomes were identified in two hares and share >99% genome-wide identity. Based on the analysis of their large T-antigen, the new polyomavirus clusters in a distant clade from other mammals sharing <64% amino acid identity. A total of 14 anelloviruses were identified, which share 63-99% genome-wide identity. Overall, our results show a coinfection of different DNA viruses in the studied samples and raise awareness regarding the extensive unsampled diversity of viruses in hares.
Bernhard Ehlers - One of the best experts on this subject based on the ideXlab platform.
-
ictv virus taxonomy profile Polyomaviridae
Journal of General Virology, 2017Co-Authors: Ugo Moens, Torbjörn Ramqvist, Sebastien Calvignacspencer, Chris Lauber, Mariet C W Feltkamp, Matthew D Daugherty, Ernst J Verschoor, Bernhard EhlersAbstract:The Polyomaviridae is a family of small, non-enveloped viruses with circular dsDNA genomes of approximately 5 kbp. The family includes four genera whose members have restricted host range, infecting mammals and birds. Polyomavirus genomes have also been detected recently in fish. Merkel cell polyomavirus and raccoon polyomavirus are associated with cancer in their host; other members are human and veterinary pathogens. Clinical manifestations are obvious in immunocompromised patients but not in healthy individuals. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Polyomaviridae, which is available at www.ictv.global/report/Polyomaviridae.
-
assessing host virus codivergence for close relatives of merkel cell polyomavirus infecting african great apes
Journal of Virology, 2016Co-Authors: Nadege Freda Madinda, Bernhard Ehlers, Joel O Wertheim, Chantal Akouakoffi, Richard A Bergl, Christophe Boesch, Dieudonne Boji Mungu Akonkwa, Winnie Eckardt, Barbara Fruth, Thomas R GillespieAbstract:It has long been hypothesized that polyomaviruses (PyV;family Polyomaviridae) codiverged with their animal hosts. In contrast, recent analyses suggested that codivergence may only marginally influence the evolution of PyV. We reassess this question by focusing on a single lineage of PyV infecting hominine hosts, the Merkel cell polyomavirus (MCPyV) lineage. By characterizing the genetic diversity of these viruses in seven African great ape taxa, we show that they exhibit very strong host specificity. Reconciliation analyses identify more codivergence than noncodivergence events. In addition, we find that a number of host and PyV divergence events are synchronous. Collectively, our results support codivergence as the dominant process at play during the evolution of the MCPyV lineage. More generally, our results add to the growing body of evidence suggesting an ancient and stable association of PyV and their animal hosts. IMPORTANCE The processes involved in viral evolution and the interaction of viruses with their hosts are of great scientific interest and public health relevance. It has long been thought that the genetic diversity of double-stranded DNA viruses was generated over long periods of time, similar to typical host evolutionary timescales. This was also hypothesized for polyomaviruses ( family Polyomaviridae), a group comprising several human pathogens, but this remains a point of controversy. Here, we investigate this question by focusing on a single lineage of polyomaviruses that infect both humans and their closest relatives, the African great apes. We show that these viruses exhibit considerable host specificity and that their evolution largely mirrors that of their hosts, suggesting that codivergence with their hosts played a major role in their diversification. Our results provide statistical evidence in favor of an association of polyomaviruses and their hosts over millions of years.
-
A taxonomy update for the family Polyomaviridae
Archives of Virology, 2016Co-Authors: Sébastien Calvignac-spencer, Ugo Moens, Torbjörn Ramqvist, Mariet C W Feltkamp, Matthew D Daugherty, Reimar Johne, Bernhard EhlersAbstract:Many distinct polyomaviruses infecting a variety of vertebrate hosts have recently been discovered, and their complete genome sequence could often be determined. To accommodate this fast-growing diversity, the International Committee on Taxonomy of Viruses (ICTV) Polyomaviridae Study Group designed a host- and sequence-based rationale for an updated taxonomy of the family Polyomaviridae . Applying this resulted in numerous recommendations of taxonomical revisions, which were accepted by the Executive Committee of the ICTV in December 2015. New criteria for definition and creation of polyomavirus species were established that were based on the observed distance between large T antigen coding sequences. Four genera ( Alpha -, Beta , Gamma - and Deltapolyomavirus ) were delineated that together include 73 species. Species naming was made as systematic as possible – most species names now consist of the binomial name of the host species followed by polyomavirus and a number reflecting the order of discovery. It is hoped that this important update of the family taxonomy will serve as a stable basis for future taxonomical developments.
-
genome sequences of murine pneumotropic virus Polyomaviridae detected in wild house mice mus musculus
Genome Announcements, 2016Co-Authors: Nicole Ben Salem, Ugo Moens, Bernhard EhlersAbstract:ABSTRACT Using generic PCR, we identified a variant of murine pneumotropic virus (MptV) (family Polyomaviridae) in 3 wild house mice (Mus musculus). The fully amplified and sequenced genomes display considerable differences from the MptV genomes published previously and enlighten us on the natural diversity of rodent polyomaviruses.
-
genome sequence of bovine polyomavirus 1 detected in a salers cow bos taurus from catalonia spain
Genome Announcements, 2016Co-Authors: Nicole Ben Salem, Ugo Moens, Bernat Perez De Val, Maite Martin, Bernhard EhlersAbstract:ABSTRACT We identified a variant of the first bovine polyomavirus (BPyV1; family Polyomaviridae) in a lymph node of a Salers cow. As the 2 previously published genome sequences of this virus originated from fetal bovine serum and ground beef, respectively, this is the first BPyV1 genome that could be traced back to an individual.