The Experts below are selected from a list of 1077 Experts worldwide ranked by ideXlab platform

Jos Hoogmartens - One of the best experts on this subject based on the ideXlab platform.

  • Hyphenation of liquid chromatography to ion trap mass spectrometry to identify minor components in Polypeptide Antibiotics
    Analytical and bioanalytical chemistry, 2003
    Co-Authors: Cindy Govaerts, Ann Van Schepdael, Erwin Adams, Jos Hoogmartens
    Abstract:

    The application of liquid chromatography–ion trap mass spectrometry for the characterization of linear and cyclic Polypeptide Antibiotics was investigated. The aim was on-line identification of impurities in those antibiotic complexes without recourse to time-consuming isolation and purification procedures. Hyphenated techniques, such as liquid chromatography coupled to mass spectrometry, are ideally suited for this purpose. Characterization was performed with an ion trap mass spectrometer offering MS n capability; this enables more structural information to be obtained. Liquid chromatography in combination with ion trap mass spectrometry was successfully applied for the characterization of impurities in gramicidin, polymyxin B, polymyxin E, and bacitracin and the study of the degradation products of polymyxins B and E.

  • sequencing of bacitracin a and related minor components by liquid chromatography electrospray ionization ion trap tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2003
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Erwin Adams, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed-phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the characterization of related compounds in commercial bacitracin samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive and negative ion mode. The LCQ ion trap is ideally suited for the sequencing of those linear side-chain cyclized peptides because it provides on-line LC/MSn capability. Using this method bacitracin A, 1-epibacitracin A, bacitracins B1, B2, B3 and bacitracin F were sequenced and previous sequencing was confirmed. Bacitracins C1, C2, C3, D, H2 and H3 were resolved chromatographically and their ring portion was sequenced for the first time. Four components not described in the literature (1-epibacitracin B1, 1-epibacitracin B2, 1-epibacitracin C1 and H4) were sequenced completely for the first time. The main advantage of this hyphenated LC/MSn technique is the characterization of the related substances without time-consuming isolation and purification procedures. Copyright © 2003 John Wiley & Sons, Ltd.

  • liquid chromatography ion trap tandem mass spectrometry for the characterization of Polypeptide Antibiotics of the colistin series in commercial samples
    Journal of Chromatography A, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed-phase liquid chromatography-tandem mass spectrometry method is described for the characterization of related substances in the colistin complex. Mass spectral data were acquired on an LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The main advantage of this technique is the characterization of novel related substances without time-consuming isolation and purification procedures. Using this method seven new related substances were partially identified in colistin bulk sample and tablets. Four components were assigned as isomers of the main components of colistin.

  • characterization of Polypeptide Antibiotics of the polymyxin series by liquid chromatography electrospray ionization ion trap tandem mass spectrometry
    Journal of Peptide Science, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Eugene Roets, Ann Van Schepdael, Jos Hoogmartens
    Abstract:

    A selective reversed phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on-line LC/MSn capability. The main advantage of this hyphenated LC/MSn technique is the characterization of novel related substances without time-consuming isolation and purification procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.

  • Characterization of Polypeptide Antibiotics of the Polymyxin Series by Liquid Chromatography Electrospray Ionization Ion Trap Tandem Mass Spectrometry
    Journal of peptide science : an official publication of the European Peptide Society, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on-line LC/MSn capability. The main advantage of this hyphenated LC/MSn technique is the characterization of novel related substances without time-consuming isolation and purifications procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample.

Cindy Govaerts - One of the best experts on this subject based on the ideXlab platform.

  • Hyphenation of liquid chromatography to ion trap mass spectrometry to identify minor components in Polypeptide Antibiotics
    Analytical and bioanalytical chemistry, 2003
    Co-Authors: Cindy Govaerts, Ann Van Schepdael, Erwin Adams, Jos Hoogmartens
    Abstract:

    The application of liquid chromatography–ion trap mass spectrometry for the characterization of linear and cyclic Polypeptide Antibiotics was investigated. The aim was on-line identification of impurities in those antibiotic complexes without recourse to time-consuming isolation and purification procedures. Hyphenated techniques, such as liquid chromatography coupled to mass spectrometry, are ideally suited for this purpose. Characterization was performed with an ion trap mass spectrometer offering MS n capability; this enables more structural information to be obtained. Liquid chromatography in combination with ion trap mass spectrometry was successfully applied for the characterization of impurities in gramicidin, polymyxin B, polymyxin E, and bacitracin and the study of the degradation products of polymyxins B and E.

  • sequencing of bacitracin a and related minor components by liquid chromatography electrospray ionization ion trap tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2003
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Erwin Adams, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed-phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the characterization of related compounds in commercial bacitracin samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive and negative ion mode. The LCQ ion trap is ideally suited for the sequencing of those linear side-chain cyclized peptides because it provides on-line LC/MSn capability. Using this method bacitracin A, 1-epibacitracin A, bacitracins B1, B2, B3 and bacitracin F were sequenced and previous sequencing was confirmed. Bacitracins C1, C2, C3, D, H2 and H3 were resolved chromatographically and their ring portion was sequenced for the first time. Four components not described in the literature (1-epibacitracin B1, 1-epibacitracin B2, 1-epibacitracin C1 and H4) were sequenced completely for the first time. The main advantage of this hyphenated LC/MSn technique is the characterization of the related substances without time-consuming isolation and purification procedures. Copyright © 2003 John Wiley & Sons, Ltd.

  • liquid chromatography ion trap tandem mass spectrometry for the characterization of Polypeptide Antibiotics of the colistin series in commercial samples
    Journal of Chromatography A, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed-phase liquid chromatography-tandem mass spectrometry method is described for the characterization of related substances in the colistin complex. Mass spectral data were acquired on an LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The main advantage of this technique is the characterization of novel related substances without time-consuming isolation and purification procedures. Using this method seven new related substances were partially identified in colistin bulk sample and tablets. Four components were assigned as isomers of the main components of colistin.

  • characterization of Polypeptide Antibiotics of the polymyxin series by liquid chromatography electrospray ionization ion trap tandem mass spectrometry
    Journal of Peptide Science, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Eugene Roets, Ann Van Schepdael, Jos Hoogmartens
    Abstract:

    A selective reversed phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on-line LC/MSn capability. The main advantage of this hyphenated LC/MSn technique is the characterization of novel related substances without time-consuming isolation and purification procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.

  • Characterization of Polypeptide Antibiotics of the Polymyxin Series by Liquid Chromatography Electrospray Ionization Ion Trap Tandem Mass Spectrometry
    Journal of peptide science : an official publication of the European Peptide Society, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on-line LC/MSn capability. The main advantage of this hyphenated LC/MSn technique is the characterization of novel related substances without time-consuming isolation and purifications procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample.

Ann Van Schepdael - One of the best experts on this subject based on the ideXlab platform.

  • Hyphenation of liquid chromatography to ion trap mass spectrometry to identify minor components in Polypeptide Antibiotics
    Analytical and bioanalytical chemistry, 2003
    Co-Authors: Cindy Govaerts, Ann Van Schepdael, Erwin Adams, Jos Hoogmartens
    Abstract:

    The application of liquid chromatography–ion trap mass spectrometry for the characterization of linear and cyclic Polypeptide Antibiotics was investigated. The aim was on-line identification of impurities in those antibiotic complexes without recourse to time-consuming isolation and purification procedures. Hyphenated techniques, such as liquid chromatography coupled to mass spectrometry, are ideally suited for this purpose. Characterization was performed with an ion trap mass spectrometer offering MS n capability; this enables more structural information to be obtained. Liquid chromatography in combination with ion trap mass spectrometry was successfully applied for the characterization of impurities in gramicidin, polymyxin B, polymyxin E, and bacitracin and the study of the degradation products of polymyxins B and E.

  • sequencing of bacitracin a and related minor components by liquid chromatography electrospray ionization ion trap tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2003
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Erwin Adams, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed-phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the characterization of related compounds in commercial bacitracin samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive and negative ion mode. The LCQ ion trap is ideally suited for the sequencing of those linear side-chain cyclized peptides because it provides on-line LC/MSn capability. Using this method bacitracin A, 1-epibacitracin A, bacitracins B1, B2, B3 and bacitracin F were sequenced and previous sequencing was confirmed. Bacitracins C1, C2, C3, D, H2 and H3 were resolved chromatographically and their ring portion was sequenced for the first time. Four components not described in the literature (1-epibacitracin B1, 1-epibacitracin B2, 1-epibacitracin C1 and H4) were sequenced completely for the first time. The main advantage of this hyphenated LC/MSn technique is the characterization of the related substances without time-consuming isolation and purification procedures. Copyright © 2003 John Wiley & Sons, Ltd.

  • liquid chromatography ion trap tandem mass spectrometry for the characterization of Polypeptide Antibiotics of the colistin series in commercial samples
    Journal of Chromatography A, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed-phase liquid chromatography-tandem mass spectrometry method is described for the characterization of related substances in the colistin complex. Mass spectral data were acquired on an LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The main advantage of this technique is the characterization of novel related substances without time-consuming isolation and purification procedures. Using this method seven new related substances were partially identified in colistin bulk sample and tablets. Four components were assigned as isomers of the main components of colistin.

  • Characterization of Polypeptide Antibiotics of the Polymyxin Series by Liquid Chromatography Electrospray Ionization Ion Trap Tandem Mass Spectrometry
    Journal of peptide science : an official publication of the European Peptide Society, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on-line LC/MSn capability. The main advantage of this hyphenated LC/MSn technique is the characterization of novel related substances without time-consuming isolation and purifications procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample.

J A Orwa - One of the best experts on this subject based on the ideXlab platform.

  • sequencing of bacitracin a and related minor components by liquid chromatography electrospray ionization ion trap tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2003
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Erwin Adams, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed-phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the characterization of related compounds in commercial bacitracin samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive and negative ion mode. The LCQ ion trap is ideally suited for the sequencing of those linear side-chain cyclized peptides because it provides on-line LC/MSn capability. Using this method bacitracin A, 1-epibacitracin A, bacitracins B1, B2, B3 and bacitracin F were sequenced and previous sequencing was confirmed. Bacitracins C1, C2, C3, D, H2 and H3 were resolved chromatographically and their ring portion was sequenced for the first time. Four components not described in the literature (1-epibacitracin B1, 1-epibacitracin B2, 1-epibacitracin C1 and H4) were sequenced completely for the first time. The main advantage of this hyphenated LC/MSn technique is the characterization of the related substances without time-consuming isolation and purification procedures. Copyright © 2003 John Wiley & Sons, Ltd.

  • liquid chromatography ion trap tandem mass spectrometry for the characterization of Polypeptide Antibiotics of the colistin series in commercial samples
    Journal of Chromatography A, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed-phase liquid chromatography-tandem mass spectrometry method is described for the characterization of related substances in the colistin complex. Mass spectral data were acquired on an LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The main advantage of this technique is the characterization of novel related substances without time-consuming isolation and purification procedures. Using this method seven new related substances were partially identified in colistin bulk sample and tablets. Four components were assigned as isomers of the main components of colistin.

  • characterization of Polypeptide Antibiotics of the polymyxin series by liquid chromatography electrospray ionization ion trap tandem mass spectrometry
    Journal of Peptide Science, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Eugene Roets, Ann Van Schepdael, Jos Hoogmartens
    Abstract:

    A selective reversed phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on-line LC/MSn capability. The main advantage of this hyphenated LC/MSn technique is the characterization of novel related substances without time-consuming isolation and purification procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.

  • Characterization of Polypeptide Antibiotics of the Polymyxin Series by Liquid Chromatography Electrospray Ionization Ion Trap Tandem Mass Spectrometry
    Journal of peptide science : an official publication of the European Peptide Society, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on-line LC/MSn capability. The main advantage of this hyphenated LC/MSn technique is the characterization of novel related substances without time-consuming isolation and purifications procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample.

Eugene Roets - One of the best experts on this subject based on the ideXlab platform.

  • sequencing of bacitracin a and related minor components by liquid chromatography electrospray ionization ion trap tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2003
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Erwin Adams, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed-phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the characterization of related compounds in commercial bacitracin samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive and negative ion mode. The LCQ ion trap is ideally suited for the sequencing of those linear side-chain cyclized peptides because it provides on-line LC/MSn capability. Using this method bacitracin A, 1-epibacitracin A, bacitracins B1, B2, B3 and bacitracin F were sequenced and previous sequencing was confirmed. Bacitracins C1, C2, C3, D, H2 and H3 were resolved chromatographically and their ring portion was sequenced for the first time. Four components not described in the literature (1-epibacitracin B1, 1-epibacitracin B2, 1-epibacitracin C1 and H4) were sequenced completely for the first time. The main advantage of this hyphenated LC/MSn technique is the characterization of the related substances without time-consuming isolation and purification procedures. Copyright © 2003 John Wiley & Sons, Ltd.

  • liquid chromatography ion trap tandem mass spectrometry for the characterization of Polypeptide Antibiotics of the colistin series in commercial samples
    Journal of Chromatography A, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed-phase liquid chromatography-tandem mass spectrometry method is described for the characterization of related substances in the colistin complex. Mass spectral data were acquired on an LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The main advantage of this technique is the characterization of novel related substances without time-consuming isolation and purification procedures. Using this method seven new related substances were partially identified in colistin bulk sample and tablets. Four components were assigned as isomers of the main components of colistin.

  • characterization of Polypeptide Antibiotics of the polymyxin series by liquid chromatography electrospray ionization ion trap tandem mass spectrometry
    Journal of Peptide Science, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Eugene Roets, Ann Van Schepdael, Jos Hoogmartens
    Abstract:

    A selective reversed phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on-line LC/MSn capability. The main advantage of this hyphenated LC/MSn technique is the characterization of novel related substances without time-consuming isolation and purification procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample. Copyright © 2002 European Peptide Society and John Wiley & Sons, Ltd.

  • Characterization of Polypeptide Antibiotics of the Polymyxin Series by Liquid Chromatography Electrospray Ionization Ion Trap Tandem Mass Spectrometry
    Journal of peptide science : an official publication of the European Peptide Society, 2002
    Co-Authors: Cindy Govaerts, J A Orwa, Ann Van Schepdael, Eugene Roets, Jos Hoogmartens
    Abstract:

    A selective reversed phase liquid chromatography/mass spectrometry (LC/MSn) method is described for the identification of related compounds in commercial polymyxin B samples. Mass spectral data for these Polypeptide Antibiotics were acquired on a LCQ ion trap mass spectrometer equipped with an electrospray ionization probe operated in the positive ion mode. The LCQ ion trap is ideally suited for the identification of the related substances because it provides on-line LC/MSn capability. The main advantage of this hyphenated LC/MSn technique is the characterization of novel related substances without time-consuming isolation and purifications procedures. Using this method six novel related substances were partially identified in a polymyxin B bulk sample.