The Experts below are selected from a list of 612 Experts worldwide ranked by ideXlab platform

Richard L. Whelan - One of the best experts on this subject based on the ideXlab platform.

  • PEriopErativE PolyphEnon E- and siliphos-inhibitEd colorEctal tumor growth and mEtastasEs without impairmEnt of gastric or abdominal wound hEaling in mousE modEls
    Surgical Endoscopy, 2012
    Co-Authors: Xiaohong Yan, Thomas R. Gardner, Michael Grieco, Sonali A. C. Herath, Joon Ho Jang, Daniel D. Kirchoff, Linda Njoh, Samer Naffouje, H. M. C. Shantha Kumara, Richard L. Whelan
    Abstract:

    Introduction PEriopErativE anticancEr thErapy that doEs not impair wound hEaling is nEEdEd to countEr thE pErsistEnt proangiogEnic plasma compositional changEs that occur aftEr colorEctal rEsEction. PolyphEnon E (PolyE), a grEEn tEa dErivativE (main componEnt EGCG), and Siliphos (main componEnt silibinin), from thE milk thistlE plant, both havE antitumor EffEcts. This study assEssEd thE impact of PolyE/Siliphos (PES) on wound hEaling and thE growth of CT-26 colon cancEr in sEvEral murinE modEls. MEthods OnE wound hEaling and thrEE tumor studiEs wErE pErformEd. Tumor Study (TS)1 assEssEd thE impact of PES on subcutanEous tumor growth, whErEas TS2 assEssEd PES’s impact on subcutanEous growth whEn givEn prE- and post-CO_2 pnEumopEritonEum (pnEumo), sham laparotomy, or anEsthEsia alonE. TS3 dEtErminEd thE ability of PES to limit hEpatic mEtastasEs (mEts) aftEr portal vEnous injEction of tumor cElls. In thE final study, laparotomy and gastrotomy wound hEaling wErE assEssEd sEvEral ways. BALB/c micE wErE usEd for all studiEs. ThE drugs wErE givEn via drinking watEr (PolyE) and gavagE (Siliphos), daily, for 7–9 days prEprocEdurE and for 7–21 days postopErativEly. Tumor mass, numbEr/sizE of hEpatic mEts, and prolifEration and apoptosis ratEs wErE assEssEd. ThE abdominal brEaking strEngth and EnErgy to failurE wErE mEasurEd postmortEm as was gastric bursting prEssurEs. REsults PES significantly inhibitEd subcutanEous growth in thE nonopErativE sEtting. PES also significantly dEcrEasEd thE numbEr/sizE of livEr mEts whEn givEn pEriopErativEly. Abdominal wound brEaking strEngth, EnErgy to wound failurE, and collagEn contEnt wErE not altErEd by PES; gastrotomy bursting strEngth also was not affEctEd by PES. NEithEr drug alonE had a significant impact on tumor growth. Conclusions ThE PES combination inhibitEd subcutanEous and hEpatic tumor growth yEt did not impair wound hEaling. PES holds promisE as a pEriopErativE anticancEr thErapy.

  • PEriopErativE PolyphEnon E- and siliphos-inhibitEd colorEctal tumor growth and mEtastasEs without impairmEnt of gastric or abdominal wound hEaling in mousE modEls.
    Surgical Endoscopy, 2012
    Co-Authors: Xiaohong Yan, Thomas R. Gardner, Michael Grieco, Sonali A. C. Herath, Joon Ho Jang, Daniel D. Kirchoff, Linda Njoh, H. M. C. Shantha Kumara, Samer Naffouje, Richard L. Whelan
    Abstract:

    Introduction PEriopErativE anticancEr thErapy that doEs not impair wound hEaling is nEEdEd to countEr thE pErsistEnt proangiogEnic plasma compositional changEs that occur aftEr colorEctal rEsEction. PolyphEnon E (PolyE), a grEEn tEa dErivativE (main componEnt EGCG), and Siliphos (main componEnt silibinin), from thE milk thistlE plant, both havE antitumor EffEcts. This study assEssEd thE impact of PolyE/Siliphos (PES) on wound hEaling and thE growth of CT-26 colon cancEr in sEvEral murinE modEls.

  • w1830 PolyphEnon E an Egcg containing grEEn tEa Extract that inhibits tumor growth has no impact on wound collagEn contEnt or clinical ratE of wound infEction or dEhiscEncE yEt has a mild inhibitory EffEct on hEaling as judgEd by tEnsomEtry
    Gastroenterology, 2008
    Co-Authors: Aviad Hoffman, Thomas R. Gardner, Raymond Baxter, Abu Nasar, Kumara H. Shantha, Keith Hoffman, Richard L. Whelan
    Abstract:

    Introduction: CatEchins in grEEn tEa, in particular EGCG (EpigallocatEchin-3-gallatE), inhibit tumor growth in many murinE modEls. PolyphEnon E (PolyE) is a concEntratEd mixturE of catEchins (65% EGCG) with minimal sidE affEcts in humans that has strongEr anti-cancEr EffEcts than tEa alonE. SurgEry is associatEd with incrEasEd ratEs of tumor growth and mEtastasEs postopErativEly(postop) in thE murinE sEtting. PEriopErativE (pEriop) administration of Poly E may limit surgEry's tumor stimulatory EffEcts. Prior to a PhasE 1 study, PolyE's EffEcts on wound hEaling must bE studiEd. This study's purposE was to assEss PolyE's impact on laparotomywound strEngth and collagEn contEnt. MEthods: Balb/CmicE wErE randomizEd to plain watEr (Control, n=20) or a 1% PolyE solution (n=25) for drinking;10 days latEr all micE undErwEnt sham laparotomy that was suturE closEd. MicE wErE sacrificEd on postop days (PODs) 7 or 21 and thE abdominal wall pElts wErE harvEstEd. ThE pEak forcE and total EnErgy rEquirEd to rupturE Each wound was dEtErminEd via tEnsomEtry; wound collagEn contEnt was dEtErminEd via a Sircol CollagEn assay. SErum EGCG lEvEls wErE dEtErminEd in 3 PolyE micE on POD1, 7, and 21 to vErify drug ingEstion. REsults arE prEsEntEd as mEan ± SD; data was assEssEd using thE studEnt's t-tEst and Mann-WhitnEy U-tEst (significancE, p<0.05). REsults: No wound infEctions or dEhiscEncEs wErE notEd in EithEr group. ThE mEan pEak rupturE forcE for Each group was statistically similar on POD 7 and 21. ThErE was no statistical diffErEncE in thE total EnErgy rEquirEd for rupturE on POD 7, howEvEr, on POD 21 thE total EnErgy rEquirEd in thE PolyE group (4.1 ± 0.78 N-mm) was significantly lowEr than for thE control micE (5.6 ± 1.7 N-mm; p=0.026). WhEn thE 2 groups wound collagEn rEsults on POD 7 wErE comparEd thErE was no diffErEncE notEd, thE samE was truE for thE POD 21 rEsults. EGCG was dEtEctEd in thE PolyE micE sErum on POD 1 and POD 7; on POD21 vEry low lEvEls wErE dEtEctEd. Conclusions: PEriopErativE PolyE administration was not associatEd with wound complications. ThE pEak forcE for rupturE and thE collagEn contEnt was similar at both timE points for thE 2 groups as was total EnErgy for rupturE on POD 7; total EnErgy on POD 21 was lEss for thE PolyE group. PolyE warrants furthEr study as a pEriopErativE anti-cancEr agEnt in thE cancEr sEtting.

  • W1830 PolyphEnon E, An EGCG Containing GrEEn TEa Extract That Inhibits Tumor Growth, Has No Impact On Wound CollagEn ContEnt or Clinical RatE of Wound InfEction or DEhiscEncE YEt Has a Mild Inhibitory EffEct On HEaling As JudgEd By TEnsomEtry
    Gastroenterology, 2008
    Co-Authors: Aviad Hoffman, Thomas R. Gardner, Raymond Baxter, Abu Nasar, Kumara H. Shantha, Keith Hoffman, Richard L. Whelan
    Abstract:

    Introduction: CatEchins in grEEn tEa, in particular EGCG (EpigallocatEchin-3-gallatE), inhibit tumor growth in many murinE modEls. PolyphEnon E (PolyE) is a concEntratEd mixturE of catEchins (65% EGCG) with minimal sidE affEcts in humans that has strongEr anti-cancEr EffEcts than tEa alonE. SurgEry is associatEd with incrEasEd ratEs of tumor growth and mEtastasEs postopErativEly(postop) in thE murinE sEtting. PEriopErativE (pEriop) administration of Poly E may limit surgEry's tumor stimulatory EffEcts. Prior to a PhasE 1 study, PolyE's EffEcts on wound hEaling must bE studiEd. This study's purposE was to assEss PolyE's impact on laparotomywound strEngth and collagEn contEnt. MEthods: Balb/CmicE wErE randomizEd to plain watEr (Control, n=20) or a 1% PolyE solution (n=25) for drinking;10 days latEr all micE undErwEnt sham laparotomy that was suturE closEd. MicE wErE sacrificEd on postop days (PODs) 7 or 21 and thE abdominal wall pElts wErE harvEstEd. ThE pEak forcE and total EnErgy rEquirEd to rupturE Each wound was dEtErminEd via tEnsomEtry; wound collagEn contEnt was dEtErminEd via a Sircol CollagEn assay. SErum EGCG lEvEls wErE dEtErminEd in 3 PolyE micE on POD1, 7, and 21 to vErify drug ingEstion. REsults arE prEsEntEd as mEan ± SD; data was assEssEd using thE studEnt's t-tEst and Mann-WhitnEy U-tEst (significancE, p

Thomas R. Gardner - One of the best experts on this subject based on the ideXlab platform.

  • PEriopErativE PolyphEnon E- and siliphos-inhibitEd colorEctal tumor growth and mEtastasEs without impairmEnt of gastric or abdominal wound hEaling in mousE modEls
    Surgical Endoscopy, 2012
    Co-Authors: Xiaohong Yan, Thomas R. Gardner, Michael Grieco, Sonali A. C. Herath, Joon Ho Jang, Daniel D. Kirchoff, Linda Njoh, Samer Naffouje, H. M. C. Shantha Kumara, Richard L. Whelan
    Abstract:

    Introduction PEriopErativE anticancEr thErapy that doEs not impair wound hEaling is nEEdEd to countEr thE pErsistEnt proangiogEnic plasma compositional changEs that occur aftEr colorEctal rEsEction. PolyphEnon E (PolyE), a grEEn tEa dErivativE (main componEnt EGCG), and Siliphos (main componEnt silibinin), from thE milk thistlE plant, both havE antitumor EffEcts. This study assEssEd thE impact of PolyE/Siliphos (PES) on wound hEaling and thE growth of CT-26 colon cancEr in sEvEral murinE modEls. MEthods OnE wound hEaling and thrEE tumor studiEs wErE pErformEd. Tumor Study (TS)1 assEssEd thE impact of PES on subcutanEous tumor growth, whErEas TS2 assEssEd PES’s impact on subcutanEous growth whEn givEn prE- and post-CO_2 pnEumopEritonEum (pnEumo), sham laparotomy, or anEsthEsia alonE. TS3 dEtErminEd thE ability of PES to limit hEpatic mEtastasEs (mEts) aftEr portal vEnous injEction of tumor cElls. In thE final study, laparotomy and gastrotomy wound hEaling wErE assEssEd sEvEral ways. BALB/c micE wErE usEd for all studiEs. ThE drugs wErE givEn via drinking watEr (PolyE) and gavagE (Siliphos), daily, for 7–9 days prEprocEdurE and for 7–21 days postopErativEly. Tumor mass, numbEr/sizE of hEpatic mEts, and prolifEration and apoptosis ratEs wErE assEssEd. ThE abdominal brEaking strEngth and EnErgy to failurE wErE mEasurEd postmortEm as was gastric bursting prEssurEs. REsults PES significantly inhibitEd subcutanEous growth in thE nonopErativE sEtting. PES also significantly dEcrEasEd thE numbEr/sizE of livEr mEts whEn givEn pEriopErativEly. Abdominal wound brEaking strEngth, EnErgy to wound failurE, and collagEn contEnt wErE not altErEd by PES; gastrotomy bursting strEngth also was not affEctEd by PES. NEithEr drug alonE had a significant impact on tumor growth. Conclusions ThE PES combination inhibitEd subcutanEous and hEpatic tumor growth yEt did not impair wound hEaling. PES holds promisE as a pEriopErativE anticancEr thErapy.

  • PEriopErativE PolyphEnon E- and siliphos-inhibitEd colorEctal tumor growth and mEtastasEs without impairmEnt of gastric or abdominal wound hEaling in mousE modEls.
    Surgical Endoscopy, 2012
    Co-Authors: Xiaohong Yan, Thomas R. Gardner, Michael Grieco, Sonali A. C. Herath, Joon Ho Jang, Daniel D. Kirchoff, Linda Njoh, H. M. C. Shantha Kumara, Samer Naffouje, Richard L. Whelan
    Abstract:

    Introduction PEriopErativE anticancEr thErapy that doEs not impair wound hEaling is nEEdEd to countEr thE pErsistEnt proangiogEnic plasma compositional changEs that occur aftEr colorEctal rEsEction. PolyphEnon E (PolyE), a grEEn tEa dErivativE (main componEnt EGCG), and Siliphos (main componEnt silibinin), from thE milk thistlE plant, both havE antitumor EffEcts. This study assEssEd thE impact of PolyE/Siliphos (PES) on wound hEaling and thE growth of CT-26 colon cancEr in sEvEral murinE modEls.

  • w1830 PolyphEnon E an Egcg containing grEEn tEa Extract that inhibits tumor growth has no impact on wound collagEn contEnt or clinical ratE of wound infEction or dEhiscEncE yEt has a mild inhibitory EffEct on hEaling as judgEd by tEnsomEtry
    Gastroenterology, 2008
    Co-Authors: Aviad Hoffman, Thomas R. Gardner, Raymond Baxter, Abu Nasar, Kumara H. Shantha, Keith Hoffman, Richard L. Whelan
    Abstract:

    Introduction: CatEchins in grEEn tEa, in particular EGCG (EpigallocatEchin-3-gallatE), inhibit tumor growth in many murinE modEls. PolyphEnon E (PolyE) is a concEntratEd mixturE of catEchins (65% EGCG) with minimal sidE affEcts in humans that has strongEr anti-cancEr EffEcts than tEa alonE. SurgEry is associatEd with incrEasEd ratEs of tumor growth and mEtastasEs postopErativEly(postop) in thE murinE sEtting. PEriopErativE (pEriop) administration of Poly E may limit surgEry's tumor stimulatory EffEcts. Prior to a PhasE 1 study, PolyE's EffEcts on wound hEaling must bE studiEd. This study's purposE was to assEss PolyE's impact on laparotomywound strEngth and collagEn contEnt. MEthods: Balb/CmicE wErE randomizEd to plain watEr (Control, n=20) or a 1% PolyE solution (n=25) for drinking;10 days latEr all micE undErwEnt sham laparotomy that was suturE closEd. MicE wErE sacrificEd on postop days (PODs) 7 or 21 and thE abdominal wall pElts wErE harvEstEd. ThE pEak forcE and total EnErgy rEquirEd to rupturE Each wound was dEtErminEd via tEnsomEtry; wound collagEn contEnt was dEtErminEd via a Sircol CollagEn assay. SErum EGCG lEvEls wErE dEtErminEd in 3 PolyE micE on POD1, 7, and 21 to vErify drug ingEstion. REsults arE prEsEntEd as mEan ± SD; data was assEssEd using thE studEnt's t-tEst and Mann-WhitnEy U-tEst (significancE, p<0.05). REsults: No wound infEctions or dEhiscEncEs wErE notEd in EithEr group. ThE mEan pEak rupturE forcE for Each group was statistically similar on POD 7 and 21. ThErE was no statistical diffErEncE in thE total EnErgy rEquirEd for rupturE on POD 7, howEvEr, on POD 21 thE total EnErgy rEquirEd in thE PolyE group (4.1 ± 0.78 N-mm) was significantly lowEr than for thE control micE (5.6 ± 1.7 N-mm; p=0.026). WhEn thE 2 groups wound collagEn rEsults on POD 7 wErE comparEd thErE was no diffErEncE notEd, thE samE was truE for thE POD 21 rEsults. EGCG was dEtEctEd in thE PolyE micE sErum on POD 1 and POD 7; on POD21 vEry low lEvEls wErE dEtEctEd. Conclusions: PEriopErativE PolyE administration was not associatEd with wound complications. ThE pEak forcE for rupturE and thE collagEn contEnt was similar at both timE points for thE 2 groups as was total EnErgy for rupturE on POD 7; total EnErgy on POD 21 was lEss for thE PolyE group. PolyE warrants furthEr study as a pEriopErativE anti-cancEr agEnt in thE cancEr sEtting.

  • W1830 PolyphEnon E, An EGCG Containing GrEEn TEa Extract That Inhibits Tumor Growth, Has No Impact On Wound CollagEn ContEnt or Clinical RatE of Wound InfEction or DEhiscEncE YEt Has a Mild Inhibitory EffEct On HEaling As JudgEd By TEnsomEtry
    Gastroenterology, 2008
    Co-Authors: Aviad Hoffman, Thomas R. Gardner, Raymond Baxter, Abu Nasar, Kumara H. Shantha, Keith Hoffman, Richard L. Whelan
    Abstract:

    Introduction: CatEchins in grEEn tEa, in particular EGCG (EpigallocatEchin-3-gallatE), inhibit tumor growth in many murinE modEls. PolyphEnon E (PolyE) is a concEntratEd mixturE of catEchins (65% EGCG) with minimal sidE affEcts in humans that has strongEr anti-cancEr EffEcts than tEa alonE. SurgEry is associatEd with incrEasEd ratEs of tumor growth and mEtastasEs postopErativEly(postop) in thE murinE sEtting. PEriopErativE (pEriop) administration of Poly E may limit surgEry's tumor stimulatory EffEcts. Prior to a PhasE 1 study, PolyE's EffEcts on wound hEaling must bE studiEd. This study's purposE was to assEss PolyE's impact on laparotomywound strEngth and collagEn contEnt. MEthods: Balb/CmicE wErE randomizEd to plain watEr (Control, n=20) or a 1% PolyE solution (n=25) for drinking;10 days latEr all micE undErwEnt sham laparotomy that was suturE closEd. MicE wErE sacrificEd on postop days (PODs) 7 or 21 and thE abdominal wall pElts wErE harvEstEd. ThE pEak forcE and total EnErgy rEquirEd to rupturE Each wound was dEtErminEd via tEnsomEtry; wound collagEn contEnt was dEtErminEd via a Sircol CollagEn assay. SErum EGCG lEvEls wErE dEtErminEd in 3 PolyE micE on POD1, 7, and 21 to vErify drug ingEstion. REsults arE prEsEntEd as mEan ± SD; data was assEssEd using thE studEnt's t-tEst and Mann-WhitnEy U-tEst (significancE, p

Aviad Hoffman - One of the best experts on this subject based on the ideXlab platform.

  • w1830 PolyphEnon E an Egcg containing grEEn tEa Extract that inhibits tumor growth has no impact on wound collagEn contEnt or clinical ratE of wound infEction or dEhiscEncE yEt has a mild inhibitory EffEct on hEaling as judgEd by tEnsomEtry
    Gastroenterology, 2008
    Co-Authors: Aviad Hoffman, Thomas R. Gardner, Raymond Baxter, Abu Nasar, Kumara H. Shantha, Keith Hoffman, Richard L. Whelan
    Abstract:

    Introduction: CatEchins in grEEn tEa, in particular EGCG (EpigallocatEchin-3-gallatE), inhibit tumor growth in many murinE modEls. PolyphEnon E (PolyE) is a concEntratEd mixturE of catEchins (65% EGCG) with minimal sidE affEcts in humans that has strongEr anti-cancEr EffEcts than tEa alonE. SurgEry is associatEd with incrEasEd ratEs of tumor growth and mEtastasEs postopErativEly(postop) in thE murinE sEtting. PEriopErativE (pEriop) administration of Poly E may limit surgEry's tumor stimulatory EffEcts. Prior to a PhasE 1 study, PolyE's EffEcts on wound hEaling must bE studiEd. This study's purposE was to assEss PolyE's impact on laparotomywound strEngth and collagEn contEnt. MEthods: Balb/CmicE wErE randomizEd to plain watEr (Control, n=20) or a 1% PolyE solution (n=25) for drinking;10 days latEr all micE undErwEnt sham laparotomy that was suturE closEd. MicE wErE sacrificEd on postop days (PODs) 7 or 21 and thE abdominal wall pElts wErE harvEstEd. ThE pEak forcE and total EnErgy rEquirEd to rupturE Each wound was dEtErminEd via tEnsomEtry; wound collagEn contEnt was dEtErminEd via a Sircol CollagEn assay. SErum EGCG lEvEls wErE dEtErminEd in 3 PolyE micE on POD1, 7, and 21 to vErify drug ingEstion. REsults arE prEsEntEd as mEan ± SD; data was assEssEd using thE studEnt's t-tEst and Mann-WhitnEy U-tEst (significancE, p<0.05). REsults: No wound infEctions or dEhiscEncEs wErE notEd in EithEr group. ThE mEan pEak rupturE forcE for Each group was statistically similar on POD 7 and 21. ThErE was no statistical diffErEncE in thE total EnErgy rEquirEd for rupturE on POD 7, howEvEr, on POD 21 thE total EnErgy rEquirEd in thE PolyE group (4.1 ± 0.78 N-mm) was significantly lowEr than for thE control micE (5.6 ± 1.7 N-mm; p=0.026). WhEn thE 2 groups wound collagEn rEsults on POD 7 wErE comparEd thErE was no diffErEncE notEd, thE samE was truE for thE POD 21 rEsults. EGCG was dEtEctEd in thE PolyE micE sErum on POD 1 and POD 7; on POD21 vEry low lEvEls wErE dEtEctEd. Conclusions: PEriopErativE PolyE administration was not associatEd with wound complications. ThE pEak forcE for rupturE and thE collagEn contEnt was similar at both timE points for thE 2 groups as was total EnErgy for rupturE on POD 7; total EnErgy on POD 21 was lEss for thE PolyE group. PolyE warrants furthEr study as a pEriopErativE anti-cancEr agEnt in thE cancEr sEtting.

  • W1830 PolyphEnon E, An EGCG Containing GrEEn TEa Extract That Inhibits Tumor Growth, Has No Impact On Wound CollagEn ContEnt or Clinical RatE of Wound InfEction or DEhiscEncE YEt Has a Mild Inhibitory EffEct On HEaling As JudgEd By TEnsomEtry
    Gastroenterology, 2008
    Co-Authors: Aviad Hoffman, Thomas R. Gardner, Raymond Baxter, Abu Nasar, Kumara H. Shantha, Keith Hoffman, Richard L. Whelan
    Abstract:

    Introduction: CatEchins in grEEn tEa, in particular EGCG (EpigallocatEchin-3-gallatE), inhibit tumor growth in many murinE modEls. PolyphEnon E (PolyE) is a concEntratEd mixturE of catEchins (65% EGCG) with minimal sidE affEcts in humans that has strongEr anti-cancEr EffEcts than tEa alonE. SurgEry is associatEd with incrEasEd ratEs of tumor growth and mEtastasEs postopErativEly(postop) in thE murinE sEtting. PEriopErativE (pEriop) administration of Poly E may limit surgEry's tumor stimulatory EffEcts. Prior to a PhasE 1 study, PolyE's EffEcts on wound hEaling must bE studiEd. This study's purposE was to assEss PolyE's impact on laparotomywound strEngth and collagEn contEnt. MEthods: Balb/CmicE wErE randomizEd to plain watEr (Control, n=20) or a 1% PolyE solution (n=25) for drinking;10 days latEr all micE undErwEnt sham laparotomy that was suturE closEd. MicE wErE sacrificEd on postop days (PODs) 7 or 21 and thE abdominal wall pElts wErE harvEstEd. ThE pEak forcE and total EnErgy rEquirEd to rupturE Each wound was dEtErminEd via tEnsomEtry; wound collagEn contEnt was dEtErminEd via a Sircol CollagEn assay. SErum EGCG lEvEls wErE dEtErminEd in 3 PolyE micE on POD1, 7, and 21 to vErify drug ingEstion. REsults arE prEsEntEd as mEan ± SD; data was assEssEd using thE studEnt's t-tEst and Mann-WhitnEy U-tEst (significancE, p

Xiaohong Yan - One of the best experts on this subject based on the ideXlab platform.

  • PEriopErativE PolyphEnon E- and siliphos-inhibitEd colorEctal tumor growth and mEtastasEs without impairmEnt of gastric or abdominal wound hEaling in mousE modEls
    Surgical Endoscopy, 2012
    Co-Authors: Xiaohong Yan, Thomas R. Gardner, Michael Grieco, Sonali A. C. Herath, Joon Ho Jang, Daniel D. Kirchoff, Linda Njoh, Samer Naffouje, H. M. C. Shantha Kumara, Richard L. Whelan
    Abstract:

    Introduction PEriopErativE anticancEr thErapy that doEs not impair wound hEaling is nEEdEd to countEr thE pErsistEnt proangiogEnic plasma compositional changEs that occur aftEr colorEctal rEsEction. PolyphEnon E (PolyE), a grEEn tEa dErivativE (main componEnt EGCG), and Siliphos (main componEnt silibinin), from thE milk thistlE plant, both havE antitumor EffEcts. This study assEssEd thE impact of PolyE/Siliphos (PES) on wound hEaling and thE growth of CT-26 colon cancEr in sEvEral murinE modEls. MEthods OnE wound hEaling and thrEE tumor studiEs wErE pErformEd. Tumor Study (TS)1 assEssEd thE impact of PES on subcutanEous tumor growth, whErEas TS2 assEssEd PES’s impact on subcutanEous growth whEn givEn prE- and post-CO_2 pnEumopEritonEum (pnEumo), sham laparotomy, or anEsthEsia alonE. TS3 dEtErminEd thE ability of PES to limit hEpatic mEtastasEs (mEts) aftEr portal vEnous injEction of tumor cElls. In thE final study, laparotomy and gastrotomy wound hEaling wErE assEssEd sEvEral ways. BALB/c micE wErE usEd for all studiEs. ThE drugs wErE givEn via drinking watEr (PolyE) and gavagE (Siliphos), daily, for 7–9 days prEprocEdurE and for 7–21 days postopErativEly. Tumor mass, numbEr/sizE of hEpatic mEts, and prolifEration and apoptosis ratEs wErE assEssEd. ThE abdominal brEaking strEngth and EnErgy to failurE wErE mEasurEd postmortEm as was gastric bursting prEssurEs. REsults PES significantly inhibitEd subcutanEous growth in thE nonopErativE sEtting. PES also significantly dEcrEasEd thE numbEr/sizE of livEr mEts whEn givEn pEriopErativEly. Abdominal wound brEaking strEngth, EnErgy to wound failurE, and collagEn contEnt wErE not altErEd by PES; gastrotomy bursting strEngth also was not affEctEd by PES. NEithEr drug alonE had a significant impact on tumor growth. Conclusions ThE PES combination inhibitEd subcutanEous and hEpatic tumor growth yEt did not impair wound hEaling. PES holds promisE as a pEriopErativE anticancEr thErapy.

  • PEriopErativE PolyphEnon E- and siliphos-inhibitEd colorEctal tumor growth and mEtastasEs without impairmEnt of gastric or abdominal wound hEaling in mousE modEls.
    Surgical Endoscopy, 2012
    Co-Authors: Xiaohong Yan, Thomas R. Gardner, Michael Grieco, Sonali A. C. Herath, Joon Ho Jang, Daniel D. Kirchoff, Linda Njoh, H. M. C. Shantha Kumara, Samer Naffouje, Richard L. Whelan
    Abstract:

    Introduction PEriopErativE anticancEr thErapy that doEs not impair wound hEaling is nEEdEd to countEr thE pErsistEnt proangiogEnic plasma compositional changEs that occur aftEr colorEctal rEsEction. PolyphEnon E (PolyE), a grEEn tEa dErivativE (main componEnt EGCG), and Siliphos (main componEnt silibinin), from thE milk thistlE plant, both havE antitumor EffEcts. This study assEssEd thE impact of PolyE/Siliphos (PES) on wound hEaling and thE growth of CT-26 colon cancEr in sEvEral murinE modEls.

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  • EffEct of diEtary PolyphEnon E and EGCG on lung tumorigEnEsis in A/J MicE.
    Pharmaceutical research, 2010
    Co-Authors: Qi Zhang, Jing Pan, Seto Ryota, Yukihiko Hara, Yian Wang, Ronald A. Lubet, Ming You
    Abstract:

    PurposE To comparE thE chEmoprEvEntivE Efficacy of PolyphEnon E (Poly E), (−)-EpigallocatEchin-3-gallatE (EGCG) and PolyphEnon E without EGCG (Poly E-EGCG) on thE dEvElopmEnt of bEnzo(a)pyrEnE (B(a)P)-inducEd lung tumors in A/J micE.

  • Lung cancEr inhibitory EffEct of EpigallocatEchin-3-gallatE is dEpEndEnt on its prEsEncE in a complEx mixturE (PolyphEnon E).
    Cancer prevention research (Philadelphia Pa.), 2009
    Co-Authors: Fan Mei, Qi Zhang, Seto Ryota, Yukihiko Hara, Ronald A. Lubet, Ruth Chen, Da-ren Chen, Ming You
    Abstract:

    GrEEn tEa has bEEn shown to Exhibit cancEr-prEvEntivE activitiEs in prEclinical studiEs. HowEvEr, (−)-EpigallocatEchin-3-gallatE (EGCG) alonE was shown to bE inEffEctivE in prEvEnting lung tumorigEnEsis in micE by aErosol administration. In this study, PolyphEnon E and PolyphEnon E without EGCG wErE administErEd by aErosol dElivEry to A/J micE 2 wEEks aftEr carcinogEn trEatmEnt and continuing daily throughout thE rEmaindEr of thE study (20 wEEks). An improvEd aErosol dElivEry systEm with a custom-built atomizEr, an EfficiEnt solvEnt rEmovE systEm, and a nosE-only ExposurE chambEr was usEd to providE aErosols with stablE sizE distribution. ThErE wErE no significant diffErEncEs in thE sizE distributions of PolyphEnon E and PolyphEnon E without EGCG. With a rElativEly low dosE lEvEl (4.19 mg/kg), PolyphEnon E dEcrEasEd tumor multiplicity by 53%, whErEas PolyphEnon E without EGCG at thE samE dosE failEd to inhibit lung carcinogEnEsis. ThEsE rEsults indicatE that aErosol administration can bE an EffEctivE approach in chEmoprEvEntion study, and aErosolizEd PolyphEnon E can significantly inhibit pulmonary adEnoma formation and growth in A/J micE. FurthErmorE, in aErosolizEd form, EGCG, which is thought to bE thE most activE componEnt of PolyphEnon E, has to bE prEsEnt with othEr tEa catEchins to show chEmoprEvEntivE activity on lung tumorigEnEsis.

  • Bioavailability of PolyphEnon E Flavan-3-ols in Humans with an IlEostomy
    The Journal of nutrition, 2008
    Co-Authors: Cyril Auger, Yukihiko Hara, William Mullen, Alan Crozier
    Abstract:

    To invEstigatE thE dEgrEE of absorption of flavan-3-ols in thE small intEstinE, human subjEcts with an ilEostomy ingEstEd 200 mg of PolyphEnon E, a grEEn tEa Extract, aftEr which ilEal fluid and urinE, collEctEd ovEr a 24-h pEriod, wErE analyzEd by high-pErformancE liquid chromatography with photodiodE array and mass spEctromEtric dEtEction. ThE data obtainEd indicatEd that although approximatEly 40% of flavan-3-ol intakE is rEcovErEd in ilEal fluid, substantial quantitiEs arE absorbEd in thE small intEstinE. MorEovEr, 14 urinary mEtabolitEs, comprising sulfatEs, glucuronidE, and mEthylatEd dErivativEs, wErE idEntifiEd and quantifiEd. All wErE mEtabolitEs of (Epi)catEchin or (Epi)gallocatEchin, rEprEsEnting 47 +/- 2% and 26 +/- 9%, rEspEctivEly, of thE ingEstEd parEnt compound. ThEsE high rEcovEriEs indicatE that thEsE flavan-3-ols absorbEd in thE small intEstinE arE much morE bioavailablE than most diEtary flavonoids. No 3-O-galloylatEd flavan-3-ols or thEir mEtabolitEs wErE dEtEctEd in urinE. ThE absEncE of urinary flavan-3-ol mEtabolitEs aftEr ingEstion of 200 mg of (-)-EpigallocatEchin gallatE indicatEs that thErE is no rEmoval of thE 3-O-galloyl group in vivo, and hEncE, this doEs not account for thE high urinary rEcovEry of (Epi)gallocatEchin mEtabolitEs aftEr ingEstion of PolyphEnon E. IncrEasing thE intakE of PolyphEnon E, by fEEding dosEs of 200, 500, and 1500 mg, lEd to incrEasEd urinary ExcrEtion of (Epi)catEchin mEtabolitEs but not mEtabolitEs of (Epi)gallocatEchin. CoingEstion of 200 mg of PolyphEnon E with brEad, chEEsE, or glucosE did not significantly modify thE absorption, mEtabolism, and ExcrEtion of flavan-3-ols. It doEs not nEcEssarily follow, howEvEr, that thE samE would occur whEn flavan-3-ols arE ingEstEd with morE complEx food matricEs.

  • EGCG and PolyphEnon E attEnuatE inflammation-rElatEd mousE colon carcinogEnEsis inducEd by AOM plus DDS.
    Molecular medicine reports, 2008
    Co-Authors: Yohei Shirakami, Yukihiko Hara, Masahito Shimizu, Hisashi Tsurumi, Takuji Tanaka, Hisakata Moriwaki
    Abstract:

    Chronic inflammation raisEs thE risk of thE dEvElopmEnt of colorEctal cancEr (CRC). GrEEn tEa catEchins (GTCs), which possEss anti-inflammatory propErtiEs, arE known to bE anti-carcinogEnic in a variEty of organ sitEs, including thE colorEctum. This study invEstigatEd whEthEr (-)-EpigallocatEchin gallatE (EGCG), a candidatE chEmo- prEvEntivE agEnt and major biologically-activE componEnt of grEEn tEa, and PolyphEnon E (Poly E), a mixturE of GTCs, supprEss inflammation-rElatEd colon carcinogEnEsis inducEd by azoxymEthanE (AOM) and dExtran sodium sulfatE (DSS). MalE ICR micE agEd 5 wEEks wErE givEn a singlE intrapEri- tonEal injEction of AOM (10 mg/kg body wEight), followEd by 2% (w/v) DSS in drinking watEr for 7 days to inducE colitis- rElatEd colonic tumors. ThEy also rEcEivEd drinking watEr containing EGCG (0.01 or 0.1%) or Poly E (0.01 or 0.1%) up to wEEk 17. At wEEk 17, trEatmEnt with EGCG or Poly E had significantly supprEssEd thE multiplicity and volumE of colonic nEoplasms as comparEd to thE AOM/DSS group, and had rEsultEd in a lEssEr dEgrEE of malignancy. In addition, trEat- mEnt with EGCG or Poly E dEcrEasEd thE protEin and mRNA ExprEssion lEvEls of CyclooxygEnasE (COX)-2 and thE mRNA ExprEssion of inflammatory cytokinEs (TNF-α, IFN-γ, IL-6, IL-12 and IL-18) in thE colonic mucosa. Our findings providE EvidEncE that tEa catEchins arE bEnEficial to thE supprEssion of cancEr dEvElopmEnt in thE inflamEd colon.

  • PrEvEntivE EffEcts of PolyphEnon E on urinary bladdEr and mammary cancErs in rats and corrElations with sErum and urinE lEvEls of tEa polyphEnols
    Molecular cancer therapeutics, 2007
    Co-Authors: Ronald A. Lubet, James A. Crowell, Yukihiko Hara, Chung S. Yang, Mao-jung Lee, Izet M. Kapetanovic, Vernon E. Steele, M. Margaret Juliana, Clinton J. Grubbs
    Abstract:

    PolyphEnon E, a standardizEd mixturE of grEEn tEa polyphEnols, was ExaminEd for its chEmoprEvEntivE Efficacy against chEmically inducEd urinary bladdEr and mammary cancErs. In thE prEsEnt study, PolyphEnon E was administErEd aftEr thE last dosE of 4-hydroxybutyl(butyl)nitrosaminE, or roughly 30% of thE way into thE ExpErimEnt. PolyphEnon E (100 or 250 mg/kg body wEight/d) causEd a dosE-dEpEndEnt dEcrEasE in palpablE urinary bladdEr tumors [low dosE, 14 of 34; high dosE, 6 of 35; controls, 20 of 34 (P < 0.01)]. In thE mammary cancEr modEl, PolyphEnon E [333 or 1,000 mg/kg body wEight (BW)/d] was administErEd bEginning 5 days aftEr a singlE dosE of mEthylnitrosourEa. In contrast to its significant Efficacy in bladdEr tumor prEvEntion, PolyphEnon E had a minimal EffEct in thE prEvEntion of mammary cancErs. LEvEls of polyphEnols wErE dEtErminEd in thE urinE and sErum of rats. RElativEly high lEvEls of various polyphEnols (and mEtabolitEs) wErE found in thE urinE. HowEvEr, virtually no EpigallocatEchin-3-gallatE was obsErvEd in thE urinE bEcausE of low systEmic bioavailability; although it rEprEsEnts almost 65% of thE polyphEnols in PolyphEnon E. LEvEls of polyphEnols in sErum wErE 50� to 1,000� lEss than wErE obsErvEd in urinE. ThE bioavailability of thEsE tEa polyphEnols to diffErEnt organ sitEs may contributE to thE diffEring prEvEntivE Efficacy of PolyphEnon E against urinary bladdEr and mammary cancErs. [Mol CancEr ThEr 2007;6(7):2022 – 8]