The Experts below are selected from a list of 1293 Experts worldwide ranked by ideXlab platform
Jianyong Wu - One of the best experts on this subject based on the ideXlab platform.
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heptasaccharide and octasaccharide isolated from paris Polyphylla var yunnanensis and their plant growth regulatory activity
Plant Science, 2003Co-Authors: Ligang Zhou, Chengzong Yang, Shilin Wang, Jianqiang Li, Jianyong WuAbstract:Two oliaosaccharides, heptasaccharide (HS) and octasaccharide (OS), were isolated from the water extract of the rhizomes of Paris Polyphylla var. yunnanensis. They were identified as linear oligomers, composed of glucose and mannose monomers, with the molecular formula of C42H72O36 (M = 1152 and DP = 7) for HS and C48H82O41 (M= 1214 and DP = 8) for OS (DP, degree of polymerization). Both had plant growth-regulatory activity at low concentrations, stimulating the shoot formation of P. Polyphylla var. yunnanensis in culture at 2.5-10 mg/l, and the growth and saponin formation of Panax japonicus var. major hairy roots at 1030 mg/l. This is the first report on these oligosaccharides isolated from P. Polyphylla var. yunnanensis and their plant growth regulatory activity. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
Haiyang Liu - One of the best experts on this subject based on the ideXlab platform.
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seeing the light shifting from wild rhizomes to extraction of active ingredients from above ground parts of paris Polyphylla var yunnanensis
Journal of Ethnopharmacology, 2018Co-Authors: Xujie Qin, Changxiang Chen, Haiyang LiuAbstract:Abstract Ethnopharmacological relevance The dried rhizomes of Paris Polyphylla var. yunnanensis are widely used in traditional Chinese medicine (TCM) as hemostatic, antitumor, and antimicrobial agents. More than 70 Chinese patent medicines are based on P. Polyphylla var. yunnanensis rhizomes. Steroidal saponins are considered as the main active ingredients of these rhizomes. However, wild populations of P. Polyphylla var. yunnanensis are greatly threatened due to the illegal wild harvest and over-utilization of the rhizomes. In contrast, the renewable above-ground parts (leaves and stems) of P. Polyphylla var. yunnanensis are usually thrown away as waste material, whether from wild or cultivated material. Aim of the study The aim of this study was to use HPLC analyses of chemical constituents and bioactive assays to assess whether the above-ground parts could be an alternative source of active ingredients to the rhizomes of P. Polyphylla var. yunnanensis. Materials and methods The saponin components of the rhizomes and above-ground parts of P. Polyphylla var. yunnanensis were analyzed by HPLC-UV. The total saponins extracted from the rhizomes and above-ground parts of P. Polyphylla var. yunnanensis were evaluated for their hemostatic, cytotoxic, and antimicrobial activities by using the rabbit blood in vitro based on turbidimetric method, MTT assay method, and a dilution antimicrobial susceptibility test method, respectively. Results Four bioactive spirostanol saponins (paris saponins I, II, VI, and VII) were detected in the total saponins from the rhizomes and above-ground parts of P. Polyphylla var. yunnanensis, which indicated they should have similar pharmacological properties. The bioactive assays revealed that both the parts of P. Polyphylla var. yunnanensis exhibited the same hemostatic, cytotoxic, and antimicrobial effects. Conclusion Our results revealed that based on saponin content in the above-ground parts of P. Polyphylla var. yunnanensis and the requirements stipulated in 2015 of Chinese Pharmacopoeia, the above-ground parts (especially its leaves) can be an alternative and more sustainable source of active ingredients compared to the rhizomes.
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Untiring Researches for Alternative Resources of Rhizoma Paridis
SpringerOpen, 2018Co-Authors: Xujie Qin, Changxiang Chen, Haiyang LiuAbstract:Abstract Rhizoma Paridis (RP, 重楼), a traditional Chinese medicine, is the rhizoma of Paris Polyphylla var. yunnanensis (PPY) or P. Polyphylla var. chinensis which are widely used as important raw materials for several Chinese patent drugs. However, the wild resources of these herbs have become less and less due to their slow-growing characteristics and previously excessive excavation. This review covers untiring investigations on alternative resources of RP by our research group over the past decades, including non-medicinal parts of PPY as well as other plants of Liliaceae and Liliflorae families. The arial parts of PPY and the whole plants of Trillium kamtschaticum might be alternative resources for RP based on the fact that they shared the same or similar saponins and bioactivities
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steroidal saponins with cytotoxic activity from the rhizomes of paris Polyphylla var yunnanensis
Phytochemistry Letters, 2015Co-Authors: Xujie Qin, Changxiang Chen, Huan Yan, Yanshi Wen, Yan Hua, Yungchi Cheng, Haiyang LiuAbstract:Two previously unreported spirostanol saponins, dianchonglouosides A and B (1 and 2), along with 7 known steroidal saponins (3-9) were isolated from the rhizomes of Paris Polyphylla var. yunnanensis. Their structures were elucidated by extensive spectroscopic analysis (MS, 1D, and 2D NMR), and chemical methods. The cytotoxic activities of the isolated compounds 1-9 were also evaluated against two human cancer cell lines (HEK293 and HepG2). The results showed that compound 7 had the strongest cytotoxic activity against the two cancer cell lines with the IC50 values of 0.6 and 0.9 mM, respectively. (C) 2015 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.
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c 22 steroidal lactone glycosides from stems and leaves of paris Polyphylla var yunnanensis
Fitoterapia, 2013Co-Authors: Xujie Qin, Changxiang Chen, Huan Yan, Haiyang LiuAbstract:Further phytochemical investigation on the stems and leaves of Paris Polyphylla var. yunnanensis has led to the isolation of three C-22-steroidal lactone glycosides. Two of these are new compounds, designated as chonglouoside SL-7 (1) and chonglouoside SL-8 (2). Their structures were elucidated on the basis of extensive spectroscopic analysis, as well as comparison with the reported spectroscopic data. This is the first report of C-22-steroidal lactone glycosides isolated from the Paris genus. Compounds 1 and 3 showed moderate antimicrobic activity against Propionibacterium acnes with MIC values of 31.3 and 3.9 mu g/mL, respectively. (c) 2012 Elsevier B.V. All rights reserved.
Ligang Zhou - One of the best experts on this subject based on the ideXlab platform.
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heptasaccharide and octasaccharide isolated from paris Polyphylla var yunnanensis and their plant growth regulatory activity
Plant Science, 2003Co-Authors: Ligang Zhou, Chengzong Yang, Shilin Wang, Jianqiang Li, Jianyong WuAbstract:Two oliaosaccharides, heptasaccharide (HS) and octasaccharide (OS), were isolated from the water extract of the rhizomes of Paris Polyphylla var. yunnanensis. They were identified as linear oligomers, composed of glucose and mannose monomers, with the molecular formula of C42H72O36 (M = 1152 and DP = 7) for HS and C48H82O41 (M= 1214 and DP = 8) for OS (DP, degree of polymerization). Both had plant growth-regulatory activity at low concentrations, stimulating the shoot formation of P. Polyphylla var. yunnanensis in culture at 2.5-10 mg/l, and the growth and saponin formation of Panax japonicus var. major hairy roots at 1030 mg/l. This is the first report on these oligosaccharides isolated from P. Polyphylla var. yunnanensis and their plant growth regulatory activity. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
Baozhong Duan - One of the best experts on this subject based on the ideXlab platform.
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authenticity analyses of rhizoma paridis using barcoding coupled with high resolution melting bar hrm analysis to control its quality for medicinal plant product
Chinese Medicine, 2018Co-Authors: Baozhong Duan, Yaping Wang, Hailan Fang, Chao Xiong, Ping Wang, Shilin ChenAbstract:Rhizoma Paridis (Chonglou) is a commonly used and precious traditional Chinese medicine. Paris Polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz. and Paris Polyphylla Smith var. chinensis (Franch.) Hara are the two main sources of Chonglou under the monograph of Rhizoma Paridis in Chinese Pharmacopoeia. In the local marketplace, however, this medicine is prone to be accidentally contaminated, deliberately substituted or admixed with other species that are similar to Rhizoma Paridis in shape and color. Consequently, these adulterations might compromise quality control and result in considerable health concerns for consumers. This study aims to develop a rapid and sensitive method for accurate identification of Rhizoma Paridis and its common adulterants. DNA barcoding coupled with high resolution melting analysis was applied in this research to distinguish Rhizoma Paridis from its adulteration. The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. DNA of the tested herbal medicines was isolated and their melting profiles were generated and compared with the standard melting profile of P. Polyphylla var. chinensis. The results indicate that the ITS2 molecular regions coupled with HRM analysis can effectively differentiate nine herbal species, including two authentic origins of Chonglou and their seven common adulterants. Ten herbal medicines labeled “Chonglou” obtained from a local market were collected and identified with our methods, and their sequence information was analyzed to validate the accuracy of HRM analysis. DNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which could contribute to the quality control of Rhizoma Paridis in the supply chain of the natural health product industry (NHP).
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Authenticity analyses of Rhizoma Paridis using barcoding coupled with high resolution melting (Bar-HRM) analysis to control its quality for medicinal plant product
'Springer Science and Business Media LLC', 2018Co-Authors: Baozhong Duan, Yaping Wang, Hailan Fang, Chao Xiong, Ping Wang, Shilin ChenAbstract:Abstract Background Rhizoma Paridis (Chonglou) is a commonly used and precious traditional Chinese medicine. Paris Polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz. and Paris Polyphylla Smith var. chinensis (Franch.) Hara are the two main sources of Chonglou under the monograph of Rhizoma Paridis in Chinese Pharmacopoeia. In the local marketplace, however, this medicine is prone to be accidentally contaminated, deliberately substituted or admixed with other species that are similar to Rhizoma Paridis in shape and color. Consequently, these adulterations might compromise quality control and result in considerable health concerns for consumers. This study aims to develop a rapid and sensitive method for accurate identification of Rhizoma Paridis and its common adulterants. Methods DNA barcoding coupled with high resolution melting analysis was applied in this research to distinguish Rhizoma Paridis from its adulteration. The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. DNA of the tested herbal medicines was isolated and their melting profiles were generated and compared with the standard melting profile of P. Polyphylla var. chinensis. Results The results indicate that the ITS2 molecular regions coupled with HRM analysis can effectively differentiate nine herbal species, including two authentic origins of Chonglou and their seven common adulterants. Ten herbal medicines labeled “Chonglou” obtained from a local market were collected and identified with our methods, and their sequence information was analyzed to validate the accuracy of HRM analysis. Conclusions DNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which could contribute to the quality control of Rhizoma Paridis in the supply chain of the natural health product industry (NHP)
Shilin Chen - One of the best experts on this subject based on the ideXlab platform.
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authenticity analyses of rhizoma paridis using barcoding coupled with high resolution melting bar hrm analysis to control its quality for medicinal plant product
Chinese Medicine, 2018Co-Authors: Baozhong Duan, Yaping Wang, Hailan Fang, Chao Xiong, Ping Wang, Shilin ChenAbstract:Rhizoma Paridis (Chonglou) is a commonly used and precious traditional Chinese medicine. Paris Polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz. and Paris Polyphylla Smith var. chinensis (Franch.) Hara are the two main sources of Chonglou under the monograph of Rhizoma Paridis in Chinese Pharmacopoeia. In the local marketplace, however, this medicine is prone to be accidentally contaminated, deliberately substituted or admixed with other species that are similar to Rhizoma Paridis in shape and color. Consequently, these adulterations might compromise quality control and result in considerable health concerns for consumers. This study aims to develop a rapid and sensitive method for accurate identification of Rhizoma Paridis and its common adulterants. DNA barcoding coupled with high resolution melting analysis was applied in this research to distinguish Rhizoma Paridis from its adulteration. The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. DNA of the tested herbal medicines was isolated and their melting profiles were generated and compared with the standard melting profile of P. Polyphylla var. chinensis. The results indicate that the ITS2 molecular regions coupled with HRM analysis can effectively differentiate nine herbal species, including two authentic origins of Chonglou and their seven common adulterants. Ten herbal medicines labeled “Chonglou” obtained from a local market were collected and identified with our methods, and their sequence information was analyzed to validate the accuracy of HRM analysis. DNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which could contribute to the quality control of Rhizoma Paridis in the supply chain of the natural health product industry (NHP).
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Authenticity analyses of Rhizoma Paridis using barcoding coupled with high resolution melting (Bar-HRM) analysis to control its quality for medicinal plant product
'Springer Science and Business Media LLC', 2018Co-Authors: Baozhong Duan, Yaping Wang, Hailan Fang, Chao Xiong, Ping Wang, Shilin ChenAbstract:Abstract Background Rhizoma Paridis (Chonglou) is a commonly used and precious traditional Chinese medicine. Paris Polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz. and Paris Polyphylla Smith var. chinensis (Franch.) Hara are the two main sources of Chonglou under the monograph of Rhizoma Paridis in Chinese Pharmacopoeia. In the local marketplace, however, this medicine is prone to be accidentally contaminated, deliberately substituted or admixed with other species that are similar to Rhizoma Paridis in shape and color. Consequently, these adulterations might compromise quality control and result in considerable health concerns for consumers. This study aims to develop a rapid and sensitive method for accurate identification of Rhizoma Paridis and its common adulterants. Methods DNA barcoding coupled with high resolution melting analysis was applied in this research to distinguish Rhizoma Paridis from its adulteration. The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. DNA of the tested herbal medicines was isolated and their melting profiles were generated and compared with the standard melting profile of P. Polyphylla var. chinensis. Results The results indicate that the ITS2 molecular regions coupled with HRM analysis can effectively differentiate nine herbal species, including two authentic origins of Chonglou and their seven common adulterants. Ten herbal medicines labeled “Chonglou” obtained from a local market were collected and identified with our methods, and their sequence information was analyzed to validate the accuracy of HRM analysis. Conclusions DNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which could contribute to the quality control of Rhizoma Paridis in the supply chain of the natural health product industry (NHP)