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Gengyuan Tian - One of the best experts on this subject based on the ideXlab platform.

  • anti hiv activities of achyranthes bidentata Polysaccharide Sulfate in vitro and in vivo
    Acta pharmaceutica Sinica, 2008
    Co-Authors: Zonggen Peng, Hongshan Chen, Zhimin Guo, Biao Dong, Gengyuan Tian, Guangqiang Wang
    Abstract:

    Achyranthes bidentata Polysaccharide Sulfate (ABPS) was a Sulfated derivate derived from Achyranthes bidentata Polysaccharide (ABP) which was isolated and identified from Chinese herb Achyranthes bidentata. The anti human immunodeficiency virus type 1 (HIV-1) activities were studied in vitro and in vivo. ABPS was found to inhibit HIV-1 reverse transcriptase and integrase with the 50% inhibiting concentration (IC60) of (2.948 +/- 0.556) micromol x L(-1) and (0.155 +/- 0.030) micromol x L(-1), respectively, but the parent compound ABP was not effective. ABPS inhibited HIV-1 P24 antigen with IC50 of (0.082 +/- 0.044) micromol x L(-1) and selective index (SI) of > (358 +/- 148) in MT-4 cell cultures acutely infected with HIV-1 IIIB virus, and with IC50 of (11.80 +/- 5.90) micromol x L(-1) and SI of > (24.2 +/- 12.1) in PBMC cell cultures acutely infected with clinical isolated zidovudine resistant HIV-1 virus, but there was no activity even at its concentration of 500 micromol x L(-1) in latent infection of H9/HIV-1 IIIB cell cultures. 5% sera taken from rats after intraperitoneal injection from rats with ABPS 125 mg x kg(-1) once or mice with 3 mg x kg(-1) qd for 20 days effectively inhibited HIV-1 P24 in MT-4 cell cultures, but those had no inhibitory effect when given orally. The results suggested that ABPS is a promising HIV-1 inhibitor, active on HIV-1 reverse transcriptase, integrase in vitro and HIV-1 P24 antigens in cell cultures, it was well absorbed by intraperitoneal injection but poor in oral bioavailability. It warrants further study.

  • synthesis of achyranthes bidentata Polysaccharide Sulfate and its antivirus activity
    Acta pharmaceutica Sinica, 1995
    Co-Authors: Gengyuan Tian, M L Song, M S Zheng
    Abstract:

    Sulfation of Achyranthes bidentata Polysaccharide (Abps) with sulfuric acid or sulfur trioxide-pyridine or chlorosulfonic acid-pyridine was studied. A homogeneous sulfation method with good yield of 82.11% was obtained, using chlorosulfonic acid in an excess of pyridine. Sulfated Achyranthes bidentata Polysaccharide was obtained as an amorphous sodium salt easily soluble in water. The UV and IR spectrum of Abps Sulfate showed absorptions at 208, 268, 286 nm and 1232, 823.6 cm-1 respectively. The sulfur content of the products was found to be 20-22%. The degree of substitution varied from 2.8 to 3.2. It showed that the hydroxy group of Abps was almost completely esterified by chlorosulfonic acid. The Abps Sulfate was shown to have high activity as anti-HBsAg and HBeAg. It is also effective on simple herpes virus type-I.

M S Zheng - One of the best experts on this subject based on the ideXlab platform.

  • synthesis of achyranthes bidentata Polysaccharide Sulfate and its antivirus activity
    Acta pharmaceutica Sinica, 1995
    Co-Authors: Gengyuan Tian, M L Song, M S Zheng
    Abstract:

    Sulfation of Achyranthes bidentata Polysaccharide (Abps) with sulfuric acid or sulfur trioxide-pyridine or chlorosulfonic acid-pyridine was studied. A homogeneous sulfation method with good yield of 82.11% was obtained, using chlorosulfonic acid in an excess of pyridine. Sulfated Achyranthes bidentata Polysaccharide was obtained as an amorphous sodium salt easily soluble in water. The UV and IR spectrum of Abps Sulfate showed absorptions at 208, 268, 286 nm and 1232, 823.6 cm-1 respectively. The sulfur content of the products was found to be 20-22%. The degree of substitution varied from 2.8 to 3.2. It showed that the hydroxy group of Abps was almost completely esterified by chlorosulfonic acid. The Abps Sulfate was shown to have high activity as anti-HBsAg and HBeAg. It is also effective on simple herpes virus type-I.

Guangqiang Wang - One of the best experts on this subject based on the ideXlab platform.

  • anti hiv activities of achyranthes bidentata Polysaccharide Sulfate in vitro and in vivo
    Acta pharmaceutica Sinica, 2008
    Co-Authors: Zonggen Peng, Hongshan Chen, Zhimin Guo, Biao Dong, Gengyuan Tian, Guangqiang Wang
    Abstract:

    Achyranthes bidentata Polysaccharide Sulfate (ABPS) was a Sulfated derivate derived from Achyranthes bidentata Polysaccharide (ABP) which was isolated and identified from Chinese herb Achyranthes bidentata. The anti human immunodeficiency virus type 1 (HIV-1) activities were studied in vitro and in vivo. ABPS was found to inhibit HIV-1 reverse transcriptase and integrase with the 50% inhibiting concentration (IC60) of (2.948 +/- 0.556) micromol x L(-1) and (0.155 +/- 0.030) micromol x L(-1), respectively, but the parent compound ABP was not effective. ABPS inhibited HIV-1 P24 antigen with IC50 of (0.082 +/- 0.044) micromol x L(-1) and selective index (SI) of > (358 +/- 148) in MT-4 cell cultures acutely infected with HIV-1 IIIB virus, and with IC50 of (11.80 +/- 5.90) micromol x L(-1) and SI of > (24.2 +/- 12.1) in PBMC cell cultures acutely infected with clinical isolated zidovudine resistant HIV-1 virus, but there was no activity even at its concentration of 500 micromol x L(-1) in latent infection of H9/HIV-1 IIIB cell cultures. 5% sera taken from rats after intraperitoneal injection from rats with ABPS 125 mg x kg(-1) once or mice with 3 mg x kg(-1) qd for 20 days effectively inhibited HIV-1 P24 in MT-4 cell cultures, but those had no inhibitory effect when given orally. The results suggested that ABPS is a promising HIV-1 inhibitor, active on HIV-1 reverse transcriptase, integrase in vitro and HIV-1 P24 antigens in cell cultures, it was well absorbed by intraperitoneal injection but poor in oral bioavailability. It warrants further study.

Zonggen Peng - One of the best experts on this subject based on the ideXlab platform.

  • anti hiv activities of achyranthes bidentata Polysaccharide Sulfate in vitro and in vivo
    Acta pharmaceutica Sinica, 2008
    Co-Authors: Zonggen Peng, Hongshan Chen, Zhimin Guo, Biao Dong, Gengyuan Tian, Guangqiang Wang
    Abstract:

    Achyranthes bidentata Polysaccharide Sulfate (ABPS) was a Sulfated derivate derived from Achyranthes bidentata Polysaccharide (ABP) which was isolated and identified from Chinese herb Achyranthes bidentata. The anti human immunodeficiency virus type 1 (HIV-1) activities were studied in vitro and in vivo. ABPS was found to inhibit HIV-1 reverse transcriptase and integrase with the 50% inhibiting concentration (IC60) of (2.948 +/- 0.556) micromol x L(-1) and (0.155 +/- 0.030) micromol x L(-1), respectively, but the parent compound ABP was not effective. ABPS inhibited HIV-1 P24 antigen with IC50 of (0.082 +/- 0.044) micromol x L(-1) and selective index (SI) of > (358 +/- 148) in MT-4 cell cultures acutely infected with HIV-1 IIIB virus, and with IC50 of (11.80 +/- 5.90) micromol x L(-1) and SI of > (24.2 +/- 12.1) in PBMC cell cultures acutely infected with clinical isolated zidovudine resistant HIV-1 virus, but there was no activity even at its concentration of 500 micromol x L(-1) in latent infection of H9/HIV-1 IIIB cell cultures. 5% sera taken from rats after intraperitoneal injection from rats with ABPS 125 mg x kg(-1) once or mice with 3 mg x kg(-1) qd for 20 days effectively inhibited HIV-1 P24 in MT-4 cell cultures, but those had no inhibitory effect when given orally. The results suggested that ABPS is a promising HIV-1 inhibitor, active on HIV-1 reverse transcriptase, integrase in vitro and HIV-1 P24 antigens in cell cultures, it was well absorbed by intraperitoneal injection but poor in oral bioavailability. It warrants further study.

M L Song - One of the best experts on this subject based on the ideXlab platform.

  • synthesis of achyranthes bidentata Polysaccharide Sulfate and its antivirus activity
    Acta pharmaceutica Sinica, 1995
    Co-Authors: Gengyuan Tian, M L Song, M S Zheng
    Abstract:

    Sulfation of Achyranthes bidentata Polysaccharide (Abps) with sulfuric acid or sulfur trioxide-pyridine or chlorosulfonic acid-pyridine was studied. A homogeneous sulfation method with good yield of 82.11% was obtained, using chlorosulfonic acid in an excess of pyridine. Sulfated Achyranthes bidentata Polysaccharide was obtained as an amorphous sodium salt easily soluble in water. The UV and IR spectrum of Abps Sulfate showed absorptions at 208, 268, 286 nm and 1232, 823.6 cm-1 respectively. The sulfur content of the products was found to be 20-22%. The degree of substitution varied from 2.8 to 3.2. It showed that the hydroxy group of Abps was almost completely esterified by chlorosulfonic acid. The Abps Sulfate was shown to have high activity as anti-HBsAg and HBeAg. It is also effective on simple herpes virus type-I.