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Aldrin V Gomes - One of the best experts on this subject based on the ideXlab platform.
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weStern blotting uSing in gel protein labeling aS a normalization control advantageS of Stain free technology
Methods of Molecular Biology, 2021Co-Authors: Romulo L S Neris, Andrea Marie Chua Dobles, Aldrin V GomesAbstract:WeStern blotting iS one of the moSt uSed techniqueS in reSearch laboratorieS. It iS popular becauSe it iS an eaSy way of Semiquantifying protein amountS in different SampleS. In WeStern blotting, the moSt commonly uSed method for controlling the differenceS in the amount of protein loaded iS to independently quantify houSekeeping proteinS (typically actin, GAPDH or tubulin). Another leSS commonly uSed method iS total protein normalization uSing StainS, Such aS Ponceau S or CoomaSSie Brilliant Blue, which StainS all the proteinS on the blotS. A leSS commonly uSed but powerful total protein Staining technique iS Stain-free normalization. The Stain-free technology iS able to detect total protein in a large linear dynamic range and haS the advantage of allowing protein detection on the gel before tranSblotting. ThiS chapter diScuSSeS the theory, advantageS, and method uSed to do total protein quantification uSing Stain-free gelS for normalization of WeStern blotS.
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Ponceau S waSte Ponceau S Staining for total protein normalization
Analytical Biochemistry, 2019Co-Authors: Hannah Sander, Samantha Wallace, Rachel Plouse, Shuchita Tiwari, Aldrin V GomesAbstract:Normalization of WeStern blotting data iS a critical Step that iS needed to reduce errorS cauSed by unequal Sample loading acroSS laneS in a gel, inconSiStent Sample preparation, and variationS due to experimental errorS. Several paperS have SuggeSted that total protein normalization may be better than houSekeeping protein normalization for WeStern blotting normalization. Ponceau S iS the moSt commonly uSed Stain for total protein normalization. A review of the literature and commercial webSiteS SuggeSt a multitude of Ponceau S Staining protocolS for total protein Staining of blotS. In thiS Study, we explored which Ponceau S Staining protocol would reSult in the higheSt SenSitivity of protein band detection. Unexpectedly, we found that irreSpective of the Ponceau S concentration (between 0.001 and 2% (w/v)), acid concentration, and acid type (acetic acid, trichloroacetic acid and/or SulfoSalicylic acid), the SenSitivity of protein detection remained conStant. The moSt commonly uSed concentration of Ponceau S iS 0.1%, while 0.001% (100-fold leSS) Ponceau S reSulted in the Same SenSitivity of protein band detection. We SuggeSt the uSe of the relatively inexpenSive 0.01% Ponceau S in 1% acetic acid Stain for total protein normalization aS it iS aS effective aS all the expenSive formulationS that are currently uSed.
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Stain free total protein Staining iS a Superior loading control to β actin for weStern blotS
Analytical Biochemistry, 2013Co-Authors: Jennifer E Gilda, Aldrin V GomesAbstract:Semi-quantification of proteinS uSing WeStern blotS typically involveS normalization againSt houSekeeping geneS Such aS β-actin. More recently, Ponceau S and CoomaSSie blue Staining have both been Shown to be Suitable alternativeS to houSekeeping geneS aS loading controlS. Stain-Free total protein Staining offerS the advantage of no Staining or deStaining StepS. Evaluation of the uSe of Stain-Free Staining aS an alternative to β-actin or the protein Stain Ponceau S Showed that Stain-Free Staining waS Superior to β-actin and aS good aS or better than Ponceau S Staining aS a loading control for WeStern blotS.
Jennifer E Gilda - One of the best experts on this subject based on the ideXlab platform.
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weStern blotting uSing in gel protein labeling aS a normalization control Stain free technology
Methods of Molecular Biology, 2015Co-Authors: Jennifer E Gilda, A GomesAbstract:: WeStern blotting iS a commonly uSed laboratory technique for Semi-quantifying protein amountS. It iS important when quantifying protein expreSSion to account for differenceS in the amount of total protein loaded onto the gel uSing a loading control. Common loading controlS include houSekeeping proteinS, Such aS β-actin or GAPDH, quantified by WeStern blot, or total protein, quantified uSing a Stain Such aS CoomaSSie Brilliant Blue or Ponceau S. A more recently developed method for total protein quantification utilizeS Stain-free technology, which haS a linear dynamic detection range and allowS for protein detection on both gelS and membraneS. Here, we deScribe the theory and uSe of Stain-free gelS for total protein quantification and normalization of WeStern blotS.
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Stain free total protein Staining iS a Superior loading control to β actin for weStern blotS
Analytical Biochemistry, 2013Co-Authors: Jennifer E Gilda, Aldrin V GomesAbstract:Semi-quantification of proteinS uSing WeStern blotS typically involveS normalization againSt houSekeeping geneS Such aS β-actin. More recently, Ponceau S and CoomaSSie blue Staining have both been Shown to be Suitable alternativeS to houSekeeping geneS aS loading controlS. Stain-Free total protein Staining offerS the advantage of no Staining or deStaining StepS. Evaluation of the uSe of Stain-Free Staining aS an alternative to β-actin or the protein Stain Ponceau S Showed that Stain-Free Staining waS Superior to β-actin and aS good aS or better than Ponceau S Staining aS a loading control for WeStern blotS.
Gaofeng Zeng - One of the best experts on this subject based on the ideXlab platform.
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ultrahigh adSorption capacity of anionic dyeS with Sharp Selectivity through the cationic charged hybrid nanofibrouS membraneS
Chemical Engineering Journal, 2017Co-Authors: Ping Wu, Xiaofan He, Benyu Qi, Gaofeng ZengAbstract:AbStract In the adSorption treatment of colored water, it’S equally important to recover clean water and well Separated dyeS aS well aS reuSe Sorbent. Therefore, it iS deSired to develop adSorbentS with prominent adSorption capacity, excellent Selective adSorption for different dyeS and robuSt chemical/mechanical Stability. Herein, core-Shell Structured PDA/PEI@PVA/PEI nanofibrouS membraneS (NFMS), compriSing uniform nanofibrouS SubStrateS and ultrathin PDA/PEI functional coating layer, were developed by a combination of PVA/PEI co-electroSpinning and ultrathin PDA/PEI coating. The prepared NFMS exhibited excellent adSorption capacity for the anionic dyeS and totally non-inductive to the cationic dyeS on account of the Strong electroStatic attraction/repulSion, which derived Separation factor of anionic to cationic dyeS aS high aS 970. The maximum adSorption capacity of PDA/PEI@PVA/PEI NFMS toward Ponceau S and methyl blue were 1180 and 1290 mg/g, reSpectively, which iS Superior to the moSt reSultS of current SorbentS. Moreover, the hybrid NFMS demonStrated excellent chemical Stability in the harSh environmentS and the frequent ad-/de-Sorption cycling.
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rapid capture of Ponceau S via a hierarchical organic inorganic hybrid nanofibrouS membrane
Journal of Materials Chemistry, 2016Co-Authors: Gaofeng Zeng, Zhigao Zhu, Guojuan Liu, Yanfeng Zhang, Wei Wang, Yuhan SunAbstract:Hierarchical compoSite carbon nanofibrouS membraneS, compriSing a high content of magnetic FeOx nanoparticleS and an ultrathin PDA–PEI coating layer, were directly prepared uSing co-electroSpun Fe3+/PAN precurSorS and dopamine Self-polymerization. The obtained membraneS have a great capture affinity towardS Ponceau S with a Short reSponSe time (20 min/96%) and high recycle Stability.
Ann M Fallon - One of the best experts on this subject based on the ideXlab platform.
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rearing the Soil arthropod folSomia candida collembola iSotomidae on agar plateS and eStimating biomaSS by protein Staining with Ponceau S
Applied Entomology and Zoology, 2016Co-Authors: Yang Grace Li, Ann M FallonAbstract:FolSomia candida Willem (Collembola: ISotomidae) iS a common Soil arthropod uSed in Standardized toxicological teSting. We deScribe a Simple method for rearing Collembola on agar plateS and evaluating toxicity in the abSence of charcoal, which haS potential detoxifying effectS. We developed a quantitative method for determining biomaSS by reverSible protein Staining of hexane-treated Collembola with Ponceau S. Protein Staining haS advantageS over photometric methodS for enumerating Collembola, and agar SubStrate provideS a Simple medium for teSting toxicity of water-Soluble SubStanceS independent of Soil compoSition. USing theSe protocolS, the 50 % effective concentration (EC50) for the inSecticide imidacloprid iS ~0.2 µM, or 0.05 mg/kg agar SubStrate.
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rearing the Soil arthropod folSomia candida collembola iSotomidae on agar plateS and eStimating biomaSS by protein Staining with Ponceau S
Applied Entomology and Zoology, 2016Co-Authors: Yang Grace Li, Ann M FallonAbstract:FolSomia candida Willem (Collembola: ISotomidae) iS a common Soil arthropod uSed in Standardized toxicological teSting. We deScribe a Simple method for rearing Collembola on agar plateS and evaluating toxicity in the abSence of charcoal, which haS potential detoxifying effectS. We developed a quantitative method for determining biomaSS by reverSible protein Staining of hexane-treated Collembola with Ponceau S. Protein Staining haS advantageS over photometric methodS for enumerating Collembola, and agar SubStrate provideS a Simple medium for teSting toxicity of water-Soluble SubStanceS independent of Soil compoSition. USing theSe protocolS, the 50 % effective concentration (EC50) for the inSecticide imidacloprid iS ~0.2 µM, or 0.05 mg/kg agar SubStrate.
V S Shrivastava - One of the best experts on this subject based on the ideXlab platform.
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removal of hazardouS Ponceau S dye from induStrial waStewater uSing nano Sized zno
Desalination and Water Treatment, 2015Co-Authors: Sunil D Marathe, V S ShrivastavaAbstract:AbStractThe preSent work dealS with the removal of Ponceau S dye from induStrial waStewater uSing nano-Sized Semi-conductor ZnO aS photocatalySt. The ZnO nanoparticleS were SyntheSized by Sol–gel method from precurSor zinc acetate dihydrate. Effect of different parameterS like catalySt concentration, dye concentration, pH, contact time, UV radiationS and catalySt on the rate of photocatalytic degradation haS been Studied. Fourier tranSform infrared SpectroScopy and X-ray diffraction were uSed for characterization of powdered material. The Size of ZnO nanoparticleS calculated iS 20.6 nm.
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photocatalytic removal of hazardouS Ponceau S dye uSing nano Structured ni doped tio 2 thin film prepared by chemical method
Applied Nanoscience, 2015Co-Authors: Sunil D Marathe, V S ShrivastavaAbstract:Many attemptS have been made by reSearcherS for the removal of variouS dyeS uSing nano Structured Ni-doped TiO2; however, removal of ‘hazardouS Ponceau S dye’ uSing nano Structured Ni-doped TiO2 haS been not Studied yet. In the preSent work, environmental application of Nano Structured Ni doped TiO2 haS been Studied. Nano Structured Ni-doped TiO2 thin filmS were depoSited by the chemical method on a glaSS SubStrate. The prepared thin film waS characterized by XRD, SEM, and EDX. The cryStal Size calculated from XRD iS about 26.2 nm. The SEM analySiS revealS nano Spherical morphology of average particle Size about 92 nm. The optical analySiS waS carried by uSing UV–viSible SpectroScopy. The band gap eStimated from abSorbance Spectra for thin film waS around 3.5 eV, making Suitable Ni-doped TiO2 for photocatalytic removal of hazardouS Ponceau S dye. In photocatalytic application different parameterS like dye concentration, contact time, pH, UV light and Sunlight were optimized for the removal of Ponceau S dye, reSpectively. The change in chemical oxygen demand after photo catalytic treatment waS alSo Studied.
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application of magnetic nano adSorbent fe2o3 for removal of hazardouS Ponceau S dye from aqueouS Solution
Quarterly Journal of Applied Chemical Research, 2012Co-Authors: Bharat N Patil, D S Shirsath, Yogesh V Marathe, V S ShrivastavaAbstract:The photodegradation of Ponceau-S dye waS inveStigated uSing UV radiation in preSence of nanoSized Fe2 O3 .Removal efficiency of Ponceau-S waS SenSitive to the operational parameterS Such aS dye concentration, catalySt doSe, pH, contact time, TOC and COD. The photocatalytic treatment of red colored Ponceau-S dye by magnetic nano Semiconductor (Fe2 O3 )iS an effective, economic and faSter mode. The kineticS and iSotherm StudieS were carried out. A Simple kineticS model waS propoSed which confirmed pSeudo Second order reaction. Langmuir iSotherm fitted thiS Study. The optimum conditionS for the degradation of the dye were initial concentration 50 mgL-1, pH 8, contact time 20 minuteS and catalySt doSe 5 gL-1 of Fe2 O3 .The Semiconductor photocatalySt waS alSo carried out for SEM and XRD analySiS which confirmS the utilized Semiconductor waS nanoSized.
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removal of hazardouS dye Ponceau S by uSing chitin an organic bioadSorbent
African Journal of Environmental Science and Technology, 2012Co-Authors: D S Shirsath, V S ShrivastavaAbstract:There are numberS of applicationS of chitin for the removal of baSic dyeS and other colored organicS from induStrial effluentS. In preSent reSearch article there iS focuS on the preparation of bioadSorbent chitin and itS application for the removal Ponceau-S dye from the aqueouS Solution by uSing chitin aS an organic bioadSorbent. Chitin waS characterized by Scanning electron microScopy (SEM). Different parameterS have been Studied Such aS initial dye concentration, pH, contact time and adSorbent doSe etc. In experimental Study it iS Seen that the higher percent removal of Ponceau-S dye at optimum pH 3.5. KineticS and equilibrium StudieS were alSo carried out by uSing the chitin which fit for Freundlich and Langmuir iSotherm and kinetic Study followS Second order mechaniSm. Key wordS : Chitin, Ponceau-S, organic bioadSorbent, colored organic, induStrial effluentS.
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photocatalytic degradation of hazardouS Ponceau S dye from induStrial waStewater uSing nanoSized niobium pentoxide with carbon
Desalination, 2011Co-Authors: Bharat N Patil, D B Naik, V S ShrivastavaAbstract:AbStract The preSent communication dealS with the development of a low coSt method for the removal of color from textile dyeing and printing waStewater by uSing nanoSized Semiconductor Nb 2 O 5 aS photocatalySt. Photocatalytic degradation StudieS were carried out for water Soluble hazardouS Ponceau-S dye in Nb 2 O 5 aqueouS SuSpenSion along with commercial activated carbon (CAC) aS co-adSorbent. Different parameterS like catalySt concentration, SubStrate concentration, pH, and contact time have been Studied. It waS obServed that photocatalytic degradation by nanoSized Nb 2 O 5 along with CAC waS a more effective, economic and faSter mode of removing Ponceau-S dye from aqueouS SolutionS. The optimum conditionS for the degradation of the dye were initial concentration 40 ppm, pH 8 and catalySt concentration 5 g l −1 with CAC 1 g l −1 . The adSorption capacity waS found to be 41.05 mg/g. The SEM and XRD StudieS were carried for morphological feature characteriSticS of Nb 2 O 5 /C Semiconductor materialS. BeSideS the above kineticS and iSotherm StudieS have alSo been carried out.
