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Yoshihisa Yamashita - One of the best experts on this subject based on the ideXlab platform.
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Serum antibody to Porphyromonas gingivalis and periodontitis progression: the Hisayama Study
Journal of clinical periodontology, 2015Co-Authors: Kenji Takeuchi, Michiko Furuta, Toru Takeshita, Yukie Shibata, Yoshihiro Shimazaki, Sumio Akifusa, Toshiharu Ninomiya, Yutaka Kiyohara, Yoshihisa YamashitaAbstract:Aim To assess the effectiveness of serum titres of antibody to Porphyromonas gingivalis in the prediction of periodontitis progression in a community-dwelling adult population. Materials and Methods This 4-year follow-up cohort study included 183 Japanese individuals (75 men and 108 women) aged 40–49 years. The clinical attachment level was measured at two sites on all teeth present at baseline and follow-up, and periodontitis progression was defined as progression in attachment loss of ≥3 mm at ≥2 measurement sites. Serum titres of antibody to Porphyromonas gingivalis were evaluated at baseline and served as the primary predictor variable for periodontitis progression. Results Periodontitis progression was found in 8.7% subjects. In a multivariate logistic regression model, higher titres of antibody to Porphyromonas gingivalis and male gender were significantly associated with greater odds of periodontitis progression, even after adjustment for other confounding variables (titres of antibody to Porphyromonas gingivalis per 10 units: odds ratio, 1.66; 95% confidence interval, 1.16–2.36; male gender: odds ratio, 4.13; 95% confidence interval, 1.20–14.27). Conclusions Elevated serum titres of antibody to Porphyromonas gingivalis may be a risk factor for periodontitis progression.
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Porphyromonas pasteri sp. nov., isolated from human saliva.
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Mitsuo Sakamoto, Toru Takeshita, Yukie Shibata, Yoshihisa Yamashita, Moriya OhkumaAbstract:A bacterial strain, designated KUFDS01T, isolated from human saliva was characterized using a polyphasic taxonomic approach that included analysis of physiological and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. Cells of the strain were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. Growth of the strain was inhibited on medium containing 20 % bile. The 16S rRNA gene sequence analysis showed that the strain was a member of the genus Porphyromonas. Strain KUFDS01T was closely related to Porphyromonas catoniae JCM 13863T (96.6 % sequence similarity). An hsp60 gene sequence analysis indicated that strain KUFDS01T was different from P. catoniae JCM 13863T, with a sequence similarity value of 87.8 %. The major cellular fatty acids of strain KUFDS01T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0, C18 : 2ω6, 9c and C18 : 1ω9c. The DNA G+C content of strain KUFDS01T was 57.7 ± 0.66 mol%. On the basis of these data, strain KUFDS01T represents a novel species of the genus Porphyromonas, for which the name Porphyromonas pasteri sp. nov. is proposed. The type strain of P. pasteri is KUFDS01T ( = JCM 30531T = CCUG 66735T).
Daria N. Love - One of the best experts on this subject based on the ideXlab platform.
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associations amongst three feline Porphyromonas species from the gingival margin of cats during periodontal health and disease
Veterinary Microbiology, 1999Co-Authors: Jacqueline M Norris, Daria N. LoveAbstract:Abstract Digoxigenin labelled whole chromosomal DNA probes directed against three feline members of the genus Porphyromonas (P. gingivalis VPB 3492, P. circumdentaria NCTC 12469T and P. salivosa VPB 3313) were used to identify and quantify organisms in samples taken from the gingival margins of 40 domestic cats with different grades of periodontal disease. At the right upper canine tooth, the grade of periodontal disease ranged from 0 to 5 and the cfu of facultative/obligate anaerobes ranged from 5.5 × 104 to 2.0 × 106). In 38 of the 40 cats, at least one of the three Porphyromonas species was isolated and regression analysis showed that the cfu of total Porphyromonas sp. was a highly significant indicator of the grade of periodontal disease (p
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Glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities and glucose utilization by species within the genera bacteroides, Prevotella, and Porphyromonas
International Journal of Systematic Bacteriology, 1995Co-Authors: Graham D. Bailey, Daria N. LoveAbstract:Members of the genera Bacteroides, Prevotella, and Porphyromonas were investigated for their glucose 6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) activities by using spectrophotometric (SP) and alloenzyme elecrophoresis (AE) detection methods. When the SP and AE methods were compared, the AE method failed to detect activity in two of the six strains which exhibited G6PDH and 6PGDH activities as determined by the SP detection method. On the basis of the results of SP detection, Bacteroides levii ATCC 29147T(T = type strain) (G6PDH and 6PGDH negative) is not a member of the genus Bacteroides as currently defined, which reflects recent 16S rRNA placement, nor do Prevotella heparinolytica ATCC 35895T, Prevotella zoogleoformans ATCC 33285T, Porphyromonas canoris 12835T, and Porphyromonas salivosa NCTC 11632T(all G6PDH and 6PGDH positive) conform to their respective genus descriptions. When these organisms were grown in prereduced peptone-yeast extract broth containing 10% (wt/vol) glucose, the amounts of glucose remaining after 5 days were less than the amounts present initially for members of the genus Bacteroides (Bacteroides fragilis ATCC 25285Tand B. levii) and members of the genus Prevotella (Prevotella melaninogenica ATCC 25845T, Prevotella buccae ATCC 33574T, Prevotella heparinolytica, and Prevotella zoogleoformans). In addition, the glucose levels were lower after 5 days of incubation in broth media containing Porphyromonas asaccharolytica ATCC 25845Tand Porphyromonas salivosa, but not in media containing the other members of the genus Porphyromonas tested (Porphyromonas canoris, Porphyromonas circumdentaria NCTC 12469T, Porphyromonas endodontalis ATCC 35406T, and Porphyromonas gingivalis ATCC 33277T). The reductions in glucose levels were not directly related to the final pH values. Our results suggest that the descriptions of the genera Bacteroides, Prevotella, and Porphyromonas may require emendation to reflect variability in G6PDH and 6PGDH activities and glucose utilization.
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Porphyromonas macacae comb. nov., a Consequence of Bacteroides macacae Being a Senior Synonym of Porphyromonas salivosa
International Journal of Systematic Bacteriology, 1995Co-Authors: Daria N. LoveAbstract:DNA-DNA hybridization studies were performed with members of the genus Porphyromonas. Porphyromonas salivosa NCTC 11632T (T = type strain) exhibited an average level of intraspecies DNA-DNA hybridization with VPB 3313 and VPB 3444 of 95% and an average level of DNA-DNA hybridization with Bacteroides macacae ATCC 33141T of 81%. However, while the cat strains and monkey strain which I studied exhibited sufficient hybridization to be considered members of a single species, the tight DNA hybridization clustering and phenotypic differences suggested that the biovars isolated from cats and monkeys are distinct and can be recognized by colonial and growth characteristics, lipase activity, sorbitol utilization, proteinase patterns, and whole-cell protein profiles on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. As B. macacae is a senior synonym of P. salivosa, Porphyromonas macacae comb. nov. is proposed.
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Phylogenetic Analysis of Members of the Genus Porphyromonas and Description of Porphyromonas cangingivalis sp. nov. and Porphyromonas cansulci sp. nov.
International journal of systematic bacteriology, 1994Co-Authors: Matthew D. Collins, Daria N. Love, Anne Willems, J Karjalainen, A Kanervo, Benita Forsblom, E Sarkiala, G D Bailey, S. Stubbs, D I WigneyAbstract:The partial 16S rRNA gene sequences of representative strains of two groups of anaerobic, gram-negative, pigmented, asaccharolytic, rod-shaped bacteria isolated from subgingival plaque of dogs with naturally occurring periodontal disease were determined. A comparative analysis of the rRNA sequence data revealed that the two groups of organisms represent previously unknown lines of descent within the genus Porphyromonas. On the basis of our phylogenetic findings and the phenotypic distinctiveness of the organisms, two new species, Porphyromonas cangingivalis and Porphyromonas cansulci, are proposed.
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Porphyromonas canoris sp nov an asaccharolytic black pigmented species from the gingival sulcus of dogs
International Journal of Systematic and Evolutionary Microbiology, 1994Co-Authors: Daria N. Love, J Karjalainen, A Kanervo, Benita Forsblom, E Sarkiala, G D Bailey, D I Wigney, Hannele JousimiessomerAbstract:A new species, Porphyromonas canoris, is proposed for black-pigmented asaccharolytic strains isolated from subgingival plaque samples from dogs with naturally occurring periodontal disease. This bacterium is an obligately anaerobic, nonmotile, non-spore-forming, gram-negative, rod-shaped organism. On laked rabbit blood or sheep blood agar plates, colonies are light brown to greenish brown after 2 to 4 days of incubation and dark brown after 14 days of incubation. Colonies on egg yolk agar and on nonhemolyzed sheep blood agar are orange. The cells do not grow in the presence of 20% bile and have a guanine-plus-cytosine content of 49 to 51 mol%. The type strain is VPB 4878 (= NCTC 12835). The average levels of DNA-DNA hybridization between P. canoris strains and other members of the genus Porphyromonas are as follows: Porphyromonas gingivalis ATCC 33277T (T = type strain), 6.5%; Porphyromonas gingivalis cat strain VPB 3492, 5%; Porphyromonas endodontalis ATCC 35406T, 1%; Porphyromonas salivosa NCTC 11362T, 5%; and Porphyromonas circumdentaria NCTC 12469T, 6%. The level of hybridization between P. canoris NCTC 12835T DNA and Porphyromonas asaccharolytica ATCC 25260T DNA is 3%. P. canoris cells produce major amounts of acetic, propionic, isovaleric, and succinic acids and minor amounts of isobutyric and butyric acids as end products of metabolism in cooked meat medium. The major cellular fatty acid is 13-methyltetradecanoic acid (iso-C15:0). Glutamate and malate dehydrogenases are present, as are glucose-6-phosphate dehydrogenase activity (65.7 nmol mg of protein-1 min-1) and 6-phosphogluconate dehydrogenase activity (63.0 nmol mg of protein-1 min-1). P. canoris cells do not agglutinate sheep erythrocytes but exhibit brick red fluorescence at 265 nm and produce catalase.
Frédérique Barloy-hubler - One of the best experts on this subject based on the ideXlab platform.
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Porphyromonas spp. have an extensive host range in ill and healthy individuals and an unexpected environmental distribution: A systematic review and meta-analysis
Anaerobe, 2020Co-Authors: Luis Acuña-amador, Frédérique Barloy-hublerAbstract:Studies on the anaerobic bacteria Porphyromonas, mainly focused on P. gingivalis, have revealed new bacterial structures, metabolic pathways, and physiologic functionalities. Porphyromonas are mainly described as being associated with mammals and involved in chronic oral infections and secondary pathologies such as cancers or neurodegenerative diseases. In this review, we collected and analyzed information regarding Porphyromonas isolation sites and associated conditions and showed that Porphyromonas are detected in numerous pristine and anthropic environments and that their host range appears wider than previously believed, including aquatic animals, arthropods, and birds, even if their predominant hosts remain humans, pets, and farm animals. Our analyses also revealed their presence in multiple organs and in a substantial proportion of healthy contexts. Overall, the growing numbers of microbiota studies have allowed unprecedented advances in the understanding of Porphyromonas ecology but raise questions regarding their phylogenic assignment. In conclusion, this systematic and meta-analysis provides an overview of current knowledge regarding Porphyromonas ecological distribution and encourages additional research to fill the knowledge gaps to better understand their environmental distribution and inter- and intra-species transmission.
Yukie Shibata - One of the best experts on this subject based on the ideXlab platform.
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Serum antibody to Porphyromonas gingivalis and periodontitis progression: the Hisayama Study
Journal of clinical periodontology, 2015Co-Authors: Kenji Takeuchi, Michiko Furuta, Toru Takeshita, Yukie Shibata, Yoshihiro Shimazaki, Sumio Akifusa, Toshiharu Ninomiya, Yutaka Kiyohara, Yoshihisa YamashitaAbstract:Aim To assess the effectiveness of serum titres of antibody to Porphyromonas gingivalis in the prediction of periodontitis progression in a community-dwelling adult population. Materials and Methods This 4-year follow-up cohort study included 183 Japanese individuals (75 men and 108 women) aged 40–49 years. The clinical attachment level was measured at two sites on all teeth present at baseline and follow-up, and periodontitis progression was defined as progression in attachment loss of ≥3 mm at ≥2 measurement sites. Serum titres of antibody to Porphyromonas gingivalis were evaluated at baseline and served as the primary predictor variable for periodontitis progression. Results Periodontitis progression was found in 8.7% subjects. In a multivariate logistic regression model, higher titres of antibody to Porphyromonas gingivalis and male gender were significantly associated with greater odds of periodontitis progression, even after adjustment for other confounding variables (titres of antibody to Porphyromonas gingivalis per 10 units: odds ratio, 1.66; 95% confidence interval, 1.16–2.36; male gender: odds ratio, 4.13; 95% confidence interval, 1.20–14.27). Conclusions Elevated serum titres of antibody to Porphyromonas gingivalis may be a risk factor for periodontitis progression.
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Porphyromonas pasteri sp. nov., isolated from human saliva.
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Mitsuo Sakamoto, Toru Takeshita, Yukie Shibata, Yoshihisa Yamashita, Moriya OhkumaAbstract:A bacterial strain, designated KUFDS01T, isolated from human saliva was characterized using a polyphasic taxonomic approach that included analysis of physiological and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. Cells of the strain were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. Growth of the strain was inhibited on medium containing 20 % bile. The 16S rRNA gene sequence analysis showed that the strain was a member of the genus Porphyromonas. Strain KUFDS01T was closely related to Porphyromonas catoniae JCM 13863T (96.6 % sequence similarity). An hsp60 gene sequence analysis indicated that strain KUFDS01T was different from P. catoniae JCM 13863T, with a sequence similarity value of 87.8 %. The major cellular fatty acids of strain KUFDS01T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0, C18 : 2ω6, 9c and C18 : 1ω9c. The DNA G+C content of strain KUFDS01T was 57.7 ± 0.66 mol%. On the basis of these data, strain KUFDS01T represents a novel species of the genus Porphyromonas, for which the name Porphyromonas pasteri sp. nov. is proposed. The type strain of P. pasteri is KUFDS01T ( = JCM 30531T = CCUG 66735T).
Toru Takeshita - One of the best experts on this subject based on the ideXlab platform.
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Serum antibody to Porphyromonas gingivalis and periodontitis progression: the Hisayama Study
Journal of clinical periodontology, 2015Co-Authors: Kenji Takeuchi, Michiko Furuta, Toru Takeshita, Yukie Shibata, Yoshihiro Shimazaki, Sumio Akifusa, Toshiharu Ninomiya, Yutaka Kiyohara, Yoshihisa YamashitaAbstract:Aim To assess the effectiveness of serum titres of antibody to Porphyromonas gingivalis in the prediction of periodontitis progression in a community-dwelling adult population. Materials and Methods This 4-year follow-up cohort study included 183 Japanese individuals (75 men and 108 women) aged 40–49 years. The clinical attachment level was measured at two sites on all teeth present at baseline and follow-up, and periodontitis progression was defined as progression in attachment loss of ≥3 mm at ≥2 measurement sites. Serum titres of antibody to Porphyromonas gingivalis were evaluated at baseline and served as the primary predictor variable for periodontitis progression. Results Periodontitis progression was found in 8.7% subjects. In a multivariate logistic regression model, higher titres of antibody to Porphyromonas gingivalis and male gender were significantly associated with greater odds of periodontitis progression, even after adjustment for other confounding variables (titres of antibody to Porphyromonas gingivalis per 10 units: odds ratio, 1.66; 95% confidence interval, 1.16–2.36; male gender: odds ratio, 4.13; 95% confidence interval, 1.20–14.27). Conclusions Elevated serum titres of antibody to Porphyromonas gingivalis may be a risk factor for periodontitis progression.
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Porphyromonas pasteri sp. nov., isolated from human saliva.
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Mitsuo Sakamoto, Toru Takeshita, Yukie Shibata, Yoshihisa Yamashita, Moriya OhkumaAbstract:A bacterial strain, designated KUFDS01T, isolated from human saliva was characterized using a polyphasic taxonomic approach that included analysis of physiological and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. Cells of the strain were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. Growth of the strain was inhibited on medium containing 20 % bile. The 16S rRNA gene sequence analysis showed that the strain was a member of the genus Porphyromonas. Strain KUFDS01T was closely related to Porphyromonas catoniae JCM 13863T (96.6 % sequence similarity). An hsp60 gene sequence analysis indicated that strain KUFDS01T was different from P. catoniae JCM 13863T, with a sequence similarity value of 87.8 %. The major cellular fatty acids of strain KUFDS01T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0, C18 : 2ω6, 9c and C18 : 1ω9c. The DNA G+C content of strain KUFDS01T was 57.7 ± 0.66 mol%. On the basis of these data, strain KUFDS01T represents a novel species of the genus Porphyromonas, for which the name Porphyromonas pasteri sp. nov. is proposed. The type strain of P. pasteri is KUFDS01T ( = JCM 30531T = CCUG 66735T).
