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J M Grondona - One of the best experts on this subject based on the ideXlab platform.
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Continuous delivery of a monoclonal antibody against Reissner’s fiber into CSF reveals CSF-soluble Material immunorelated to the subcommissural organ in early chick embryos
Cell and Tissue Research, 2006Co-Authors: C. Hoyo-becerra, Juan Perez, Elena Miranda, M. D. López-Ávalos, P. Rojas-ríos, P. Fernández-llebrez, J M GrondonaAbstract:The subcommissural organ (SCO) is an ependymal differentiation located in the dorsal midline of the caudal diencephalon under the posterior commissure. SCO cells synthesize and release glycoproteins into the cerebrospinal fluid (CSF) forming a threadlike structure known as Reissner’s fiber (RF), which runs caudally along the ventricular cavities and the central canal of the spinal cord. Numerous monoclonal antibodies have been raised against bovine RF and the secretory Material of the SCO. For this study, we selected the 4F7 monoclonal antibody based on its cross-reactivity with chick embryo SCO glycoproteins in vivo. E4 chick embryos were injected with 4F7 hybridoma cells or with the purified monoclonal antibody into the ventricular cavity of the optic tectum. The hybridoma cells survived, synthesized and released antibody into the CSF for at least 13 days after the injection. E5 embryos injected with 4F7 antibody displayed precipitates in the CSF comprising both the monoclonal antibody and anti-RF-Positive Material. Such aggregates were never observed in control embryos injected with other monoclonal antibodies used as controls. Western blot analysis of CSF from E4-E6 embryos revealed several immunoreactive bands to anti-RF (AFRU) antibody. We also found AFRU-Positive Material bound to the apical surface of the choroid plexus primordia in E5 embryos. These and other ultrastructural evidence suggest the existence of soluble SCO-related molecules in the CSF of early chick embryos.
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continuous delivery of a monoclonal antibody against reissner s fiber into csf reveals csf soluble Material immunorelated to the subcommissural organ in early chick embryos
Cell and Tissue Research, 2006Co-Authors: Carolina Hoyobecerra, M D Lopezavalos, Juan Perez, Elena Miranda, P Rojasrios, P Fernandezllebrez, J M GrondonaAbstract:The subcommissural organ (SCO) is an ependymal differentiation located in the dorsal midline of the caudal diencephalon under the posterior commissure. SCO cells synthesize and release glycoproteins into the cerebrospinal fluid (CSF) forming a threadlike structure known as Reissner’s fiber (RF), which runs caudally along the ventricular cavities and the central canal of the spinal cord. Numerous monoclonal antibodies have been raised against bovine RF and the secretory Material of the SCO. For this study, we selected the 4F7 monoclonal antibody based on its cross-reactivity with chick embryo SCO glycoproteins in vivo. E4 chick embryos were injected with 4F7 hybridoma cells or with the purified monoclonal antibody into the ventricular cavity of the optic tectum. The hybridoma cells survived, synthesized and released antibody into the CSF for at least 13 days after the injection. E5 embryos injected with 4F7 antibody displayed precipitates in the CSF comprising both the monoclonal antibody and anti-RF-Positive Material. Such aggregates were never observed in control embryos injected with other monoclonal antibodies used as controls. Western blot analysis of CSF from E4-E6 embryos revealed several immunoreactive bands to anti-RF (AFRU) antibody. We also found AFRU-Positive Material bound to the apical surface of the choroid plexus primordia in E5 embryos. These and other ultrastructural evidence suggest the existence of soluble SCO-related molecules in the CSF of early chick embryos.
Xuechou Zhou - One of the best experts on this subject based on the ideXlab platform.
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effect of reduction agent on the performance of li3v2 po4 3 c Positive Material by one step solid state reaction
Electrochimica Acta, 2009Co-Authors: Xuechou ZhouAbstract:Abstract The effects of reduction agent on the electrochemical properties of Li 3 V 2 (PO 4 ) 3 /C Positive Materials were studied. An one-step solid-state reaction route was applied to synthesize Li 3 V 2 (PO 4 ) 3 /C samples. The humic acid, glucose and carbon were used as reduction agent. SEM images show that the particles of sample with humic acid as reduction agent merge with each other and form a porous structure, and yet the particles of sample synthesized using carbon as reduction agent are wrapped with small carbon particles and separate each other. Electrochemical tests show that the samples using humic acid and glucose as reduction agents have better cyclic performance than the one using carbon as reduction agent. At the 200th cycle, the sample using humic acid as reduction agent still keeps 145.2 mAh g −1 at 1 C charge and discharge rates. However, the sample using carbon as reduction agent shows a fast decline in capacity during cycling and has 54.5% capacity loss of the initial value after 200 cycles. The results indicate that different reduction agents can obviously affect the morphologies and electrochemical properties of the products.
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One-step synthesis of Li3V 2(PO4)3 /C Positive Material with high performance for lithium-ion batteries
Solid State Communications, 2008Co-Authors: Xuechou Zhou, Yongmei Liu, Yonglang GuoAbstract:Abstract Li3V 2(PO4)3/C Positive Material was synthesized via a one-step solid-state reaction route using humic acid as both reduction agent and carbon source. The SEM image shows that the particles merge with each other to form a porous structure. This microstructure helps the electrolyte to penetrate the Positive Materials and promotes good electronic contact among Li3V 2(PO4)3 particles. Electrochemical tests display that the Li3V 2(PO4)3/C Positive Material reaches an initial discharge capacity of 131.2 mA h g−1 at 0.2 C and exhibits a good cyclic performance at 3 C charge/discharge rates in the range of 3.0–4.3 V. The results indicate that the one-step solid-state reaction is a promising method suitable for mass production of Li3V 2(PO4)3/C Material with high electrochemical performance.
Juan Perez - One of the best experts on this subject based on the ideXlab platform.
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Continuous delivery of a monoclonal antibody against Reissner’s fiber into CSF reveals CSF-soluble Material immunorelated to the subcommissural organ in early chick embryos
Cell and Tissue Research, 2006Co-Authors: C. Hoyo-becerra, Juan Perez, Elena Miranda, M. D. López-Ávalos, P. Rojas-ríos, P. Fernández-llebrez, J M GrondonaAbstract:The subcommissural organ (SCO) is an ependymal differentiation located in the dorsal midline of the caudal diencephalon under the posterior commissure. SCO cells synthesize and release glycoproteins into the cerebrospinal fluid (CSF) forming a threadlike structure known as Reissner’s fiber (RF), which runs caudally along the ventricular cavities and the central canal of the spinal cord. Numerous monoclonal antibodies have been raised against bovine RF and the secretory Material of the SCO. For this study, we selected the 4F7 monoclonal antibody based on its cross-reactivity with chick embryo SCO glycoproteins in vivo. E4 chick embryos were injected with 4F7 hybridoma cells or with the purified monoclonal antibody into the ventricular cavity of the optic tectum. The hybridoma cells survived, synthesized and released antibody into the CSF for at least 13 days after the injection. E5 embryos injected with 4F7 antibody displayed precipitates in the CSF comprising both the monoclonal antibody and anti-RF-Positive Material. Such aggregates were never observed in control embryos injected with other monoclonal antibodies used as controls. Western blot analysis of CSF from E4-E6 embryos revealed several immunoreactive bands to anti-RF (AFRU) antibody. We also found AFRU-Positive Material bound to the apical surface of the choroid plexus primordia in E5 embryos. These and other ultrastructural evidence suggest the existence of soluble SCO-related molecules in the CSF of early chick embryos.
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continuous delivery of a monoclonal antibody against reissner s fiber into csf reveals csf soluble Material immunorelated to the subcommissural organ in early chick embryos
Cell and Tissue Research, 2006Co-Authors: Carolina Hoyobecerra, M D Lopezavalos, Juan Perez, Elena Miranda, P Rojasrios, P Fernandezllebrez, J M GrondonaAbstract:The subcommissural organ (SCO) is an ependymal differentiation located in the dorsal midline of the caudal diencephalon under the posterior commissure. SCO cells synthesize and release glycoproteins into the cerebrospinal fluid (CSF) forming a threadlike structure known as Reissner’s fiber (RF), which runs caudally along the ventricular cavities and the central canal of the spinal cord. Numerous monoclonal antibodies have been raised against bovine RF and the secretory Material of the SCO. For this study, we selected the 4F7 monoclonal antibody based on its cross-reactivity with chick embryo SCO glycoproteins in vivo. E4 chick embryos were injected with 4F7 hybridoma cells or with the purified monoclonal antibody into the ventricular cavity of the optic tectum. The hybridoma cells survived, synthesized and released antibody into the CSF for at least 13 days after the injection. E5 embryos injected with 4F7 antibody displayed precipitates in the CSF comprising both the monoclonal antibody and anti-RF-Positive Material. Such aggregates were never observed in control embryos injected with other monoclonal antibodies used as controls. Western blot analysis of CSF from E4-E6 embryos revealed several immunoreactive bands to anti-RF (AFRU) antibody. We also found AFRU-Positive Material bound to the apical surface of the choroid plexus primordia in E5 embryos. These and other ultrastructural evidence suggest the existence of soluble SCO-related molecules in the CSF of early chick embryos.
Elena Miranda - One of the best experts on this subject based on the ideXlab platform.
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Continuous delivery of a monoclonal antibody against Reissner’s fiber into CSF reveals CSF-soluble Material immunorelated to the subcommissural organ in early chick embryos
Cell and Tissue Research, 2006Co-Authors: C. Hoyo-becerra, Juan Perez, Elena Miranda, M. D. López-Ávalos, P. Rojas-ríos, P. Fernández-llebrez, J M GrondonaAbstract:The subcommissural organ (SCO) is an ependymal differentiation located in the dorsal midline of the caudal diencephalon under the posterior commissure. SCO cells synthesize and release glycoproteins into the cerebrospinal fluid (CSF) forming a threadlike structure known as Reissner’s fiber (RF), which runs caudally along the ventricular cavities and the central canal of the spinal cord. Numerous monoclonal antibodies have been raised against bovine RF and the secretory Material of the SCO. For this study, we selected the 4F7 monoclonal antibody based on its cross-reactivity with chick embryo SCO glycoproteins in vivo. E4 chick embryos were injected with 4F7 hybridoma cells or with the purified monoclonal antibody into the ventricular cavity of the optic tectum. The hybridoma cells survived, synthesized and released antibody into the CSF for at least 13 days after the injection. E5 embryos injected with 4F7 antibody displayed precipitates in the CSF comprising both the monoclonal antibody and anti-RF-Positive Material. Such aggregates were never observed in control embryos injected with other monoclonal antibodies used as controls. Western blot analysis of CSF from E4-E6 embryos revealed several immunoreactive bands to anti-RF (AFRU) antibody. We also found AFRU-Positive Material bound to the apical surface of the choroid plexus primordia in E5 embryos. These and other ultrastructural evidence suggest the existence of soluble SCO-related molecules in the CSF of early chick embryos.
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continuous delivery of a monoclonal antibody against reissner s fiber into csf reveals csf soluble Material immunorelated to the subcommissural organ in early chick embryos
Cell and Tissue Research, 2006Co-Authors: Carolina Hoyobecerra, M D Lopezavalos, Juan Perez, Elena Miranda, P Rojasrios, P Fernandezllebrez, J M GrondonaAbstract:The subcommissural organ (SCO) is an ependymal differentiation located in the dorsal midline of the caudal diencephalon under the posterior commissure. SCO cells synthesize and release glycoproteins into the cerebrospinal fluid (CSF) forming a threadlike structure known as Reissner’s fiber (RF), which runs caudally along the ventricular cavities and the central canal of the spinal cord. Numerous monoclonal antibodies have been raised against bovine RF and the secretory Material of the SCO. For this study, we selected the 4F7 monoclonal antibody based on its cross-reactivity with chick embryo SCO glycoproteins in vivo. E4 chick embryos were injected with 4F7 hybridoma cells or with the purified monoclonal antibody into the ventricular cavity of the optic tectum. The hybridoma cells survived, synthesized and released antibody into the CSF for at least 13 days after the injection. E5 embryos injected with 4F7 antibody displayed precipitates in the CSF comprising both the monoclonal antibody and anti-RF-Positive Material. Such aggregates were never observed in control embryos injected with other monoclonal antibodies used as controls. Western blot analysis of CSF from E4-E6 embryos revealed several immunoreactive bands to anti-RF (AFRU) antibody. We also found AFRU-Positive Material bound to the apical surface of the choroid plexus primordia in E5 embryos. These and other ultrastructural evidence suggest the existence of soluble SCO-related molecules in the CSF of early chick embryos.
Leslie Brooks - One of the best experts on this subject based on the ideXlab platform.
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Positive Material Identification (PMI) Capabilities in the Metals Secondary Industry: An Analysis of XRF and LIBS Handheld Analyzers
Light Metals 2019, 2019Co-Authors: Leslie Brooks, Gabrielle GaustadAbstract:Recycling is a critical part of obtaining a more circular economy. In the metals secondary industry, traditional equipment (a magnet, file, and/or grinding wheel) used to identify and sort Materials at their end of life can aid in grouping metals (i.e. Al + Mg alloys, ferrous, high temperature alloys, etc.), but they are incapable of identifying the alloy’s elemental composition; a necessity for preventing downcycling and maximizing secondary utilization rates. Handheld analyzers that utilize X-ray fluorescence (XRF) and spectroscopy (LIBS) technology may offer technological assistance that is helpful for achieving this level of analysis, often referred to as Positive Material Identification (PMI). This work tests the performance of these units under the challenging conditions present in yards (contaminated, unpolished, rugged scraps). These instruments, with their increasing safety settings, ruggedness, ease of point-click use and quick read times (for both XRF and LIBS) have significant potential, especially with ability to ID metal faster than cognitive recognition. Additionally, as unit costs of these instruments continue to decrease and the range of varying types of metal entering yards continues to widen, the return on investment becomes more immediate. However, extreme fluctuations of reported elemental compositions are being seen even when measurements have been taken in the same place consecutively; indicating that in their current state, they can inform content of Material but aren’t necessarily reliable for reporting accurate and precise compositional percentages.
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Positive Material Identification (PMI) Capabilities in the Metals Secondary Industry: An Analysis of XRF and LIBS Handheld Analyzers
Light Metals 2019, 2019Co-Authors: Leslie BrooksAbstract:Recycling Recycling is a critical part of obtaining a more circular economy Circular economy . In the metals secondary industry, traditional equipment (a magnet, file, and/or grinding Grinding wheel) used to identify and sort Materials at their end of life can aid in grouping metals (i.e. Al Al + Mg alloys, ferrous, high temperature alloys, etc.), but they are incapable of identifying the alloy Alloy ’s elemental composition; a necessity for preventing downcycling Downcycling and maximizing secondary utilization rates. Handheld analyzers Handheld analyzers that utilize X-ray fluorescence (XRF) X-ray fluorescence (XRF) and spectroscopy (LIBS Laser induced breakdown spectroscopy (LIBS) ) technology Technology may offer technological assistance that is helpful for achieving this level of analysis, often referred to as Positive Material Identification Material identification (PMI). This work tests the performance Performance of these units under the challenging conditions present in yards (contaminated, unpolished, rugged scraps). These instruments, with their increasing safety Safety settings, ruggedness, ease of point-click use and quick read times ( for both XRF X-ray fluorescence (XRF) and LIBS Laser induced breakdown spectroscopy (LIBS) ) have significant potential, especially with ability to ID metal faster than cognitive recognition. Additionally, as unit costs of these instruments continue to decrease and the range of varying types of metal entering yards continues to widen, the return on investment becomes more immediate. However, extreme fluctuations of reported elemental compositions are being seen even when measurements Measurements have been taken in the same place consecutively; indicating that in their current state, they can inform content of Material but aren’t necessarily reliable for reporting accurate and precise compositional percentages.
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Potential for Handheld Analyzers to Address Emerging Positive Material Identification (PMI) Challenges
The Minerals Metals & Materials Series, 2018Co-Authors: Leslie Brooks, Teija Mortvedt, Gabrielle Gaustad, Adam J. GesingAbstract:Positive Material identification (PMI) is a challenge in the metals secondary industry that remains persistent as products are designed with increasing complexity. Strategies in the automotive industry to improve fuel efficiency include development of lightweight wrought aluminum and magnesium alloys to replace heavier steel components in vehicles. These alloys have tight compositional requirements and the traditional equipment for sorting and identifying this scrap (i.e. a magnet, file, and/or grinding wheel) is inadequate. Handheld analyzers that utilize X-ray fluorescence and spectroscopy technology may offer technological assistance that is helpful for PMI. This work tests the performance of these units under the challenging conditions present in yards (contaminated, unpolished, comingled scraps). As the costs of these units have decreased substantially over time, results have shown there are more opportunities for quicker return on investment under a variety of scenarios; yard volume, incoming grades, and diversity of suppliers have the largest impact on pay-off.
