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Kenneth D. Beaman - One of the best experts on this subject based on the ideXlab platform.
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EXPRESSION OF MEMBRANE FORM OF THE Pregnancy Associated Protein TJ6 ON DECIDUAL LYMPHOCYTES IN THE FIRST TRIMESTER OF Pregnancy
Journal of reproductive immunology, 1996Co-Authors: Gordana Rubeša, Alan E. Beer, Kenneth D. Beaman, Herman Haller, Daniel RukavinaAbstract:TJ6, a newly described Protein produced locally in the uterine decidua during Pregnancy, may be involved in maintaining a unique immunological environment at the maternal-fetal interface. The aim of this study was to determine whether TJ6 is expressed as membrane form on decidual lymphocytes (DL), to define the phenotypes of TJ6m (membrane form TJ6) expressing cells and to analyze the fluorescence intensity of TJ6m expression. Peripheral blood lymphocytes (PBL) and DL were obtained from first trimester pregnancies undergoing elective termination and immunophenotyped for TJ6m and other cell surface antigens (CD3, CD8, CD19, CD56, CD16) by flow cytometry. This is the first study showing that TJ6 molecules are present on decidual lymphocytes in human Pregnancy. TJ6m expression on PBL was not different from that of DL. However, a significantly higher percentage of double positive (TJ6m+CD3+, TJ6m+,CD8+,TJ6m+CD19+) cells were found in PBL when compared to DL. The average fluorescence intensity (AFI) for the TJ6m marker among cells with CD8+, CD19+ and CD56+ double positive was significantly higher in DL as compared with those of PBL. The AFI for granularity of double positive DL was significantly higher than observed in PBL.
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Purification and characterization of a Pregnancy-Associated Protein: TJ6s.
American Journal of Reproductive Immunology, 1995Co-Authors: Malay Mandal, Kenneth D. BeamanAbstract:PROBLEM: Characterization of the soluble form of a novel Protein, TJ6 (TJ6s) with immune suppressive activity from murine fetoplacental units. METHOD: Preferential ammonium sulfate precipitation, gel filtration and ion-exchange chromatography were employed to purify the Protein TJ6s from murine fetoplacental units using an anti-peptide antibody as a detection tool. Biological activity of the purified Protein was studied in lymphocyte proliferation assays. RESULTS: Purified TJ6s has a Mr of approximately 18 kDa as evidenced by SDS-PAGE in both reducing and non reducing conditions. It exerted a strong anti-proliferative activity in both anti-CD3 and Con A proliferation lymphocyte proliferation assays but not in a PHA assay, suggesting that the anti-proliferative effects on T cells are exerted only on cells specifically activated directly through T cell receptor complex. CONCLUSION: The results indicate that TJ6s is a novel anti-proliferative Protein that has many of the characteristics that are considered necessary for survival of the fetal allograft
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Expression of a membrane form of the Pregnancy-Associated Protein TJ6 on lymphocytes
Cellular Immunology, 1994Co-Authors: Timothy C. Nichols, Jeong-ah Kang, Vachras Angkachatchai, Alan E. Beer, Kenneth D. BeamanAbstract:Abstract TJ6 is a novel Protein which has immunosuppressive activity and may have a functional role in fetal allograft survival during Pregnancy. Initial studies indicated that when mice were treated with an anti-TJ6 binding mAb early in Pregnancy, the pregnancies were completely ablated and that TJ6 expression is enhanced dramatically during Pregnancy. In addition we have cloned the cDNA for TJ6 which encodes a possible transmembrane domain that may include six to seven transmembrane regions. Therefore, we examined TJ6 expression on PBL of pregnant and nonpregnant women and found that TJ6 is expressed primarily on CD19 + B cells from pregnant but not nonpregnant women. TJ6 was not expressed on CD3 + lymphocytes from either group but was expressed on CD56 + cells from a small population of pregnant women which preliminary data indicate may correlate with the occurrence of spontaneous abortion in these women. Here we also show that TJ6 transcripts are highly expressed in the developing fetoplacental unit as well as in the developing thymus. We also begin to characterize the expression of TJ6 isoforms in an acute lymphocytic leukemia cell line (SB), murine thymus, and the developing murine fetoplacental unit, as well as the expression of a membrane form of TJ6 present on human lymphocytes during Pregnancy. All of these cells and tissues expressed TJ6 Proteins which were smaller than predicted based on either the cDNA sequence or the in vitro translation even though they all expressed mRNA similar in size. The TJ6 isoforms varied in size from the 45-kDa isoform in SB cells to the 52-kDa isoform of the fetoplacental unit to a 70-kDa isoform in murine thymus. Flow cytometric analysis also demonstrated that similar to the CD19 + B cells from pregnant women, TJ6 is expressed on the surface of SB cells.
M. Johnchapman - One of the best experts on this subject based on the ideXlab platform.
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Serum Plasma Pregnancy-Associated Protein A
Arteriosclerosis Thrombosis and Vascular Biology, 2003Co-Authors: Jean-louisbeaudeux, Laurenceburc, Françoiseimbert-bismut, Philippegiral, Maguybernard, Ericbruckert, M. JohnchapmanAbstract:Objective— The proteolytic activity of metalloProteinases, proinflammatory enzymes that degrade extracellular matrix, is elevated in lipid-rich atherosclerotic plaques, thereby contributing to plaque fragility and rupture. Pregnancy-Associated plasma Protein (PAPP-A) is a metalloProteinase, expressed in unstable atherosclerotic plaques, whose circulating levels are elevated in acute coronary syndromes. We evaluated serum PAPP-A levels as a marker of the premature development of atherosclerosis in hyperlipidemic subjects at elevated cardiovascular risk. Methods and Results— Serum PAPP-A levels were determined in asymptomatic hyperlipidemic male subjects (n=64; mean±SD age, 51±7 years) in whom intima-media thickness (IMT) and lesion status in the carotid artery were evaluated by noninvasive ultrasonography and compared with those of a normolipidemic control group (n=25). No difference was observed in circulating PAPP-A levels between hyperlipidemic subjects and controls (8.99±2.93 and 8.03±2.75 mIU/L, respe...
Nicole Chene - One of the best experts on this subject based on the ideXlab platform.
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Functions of embryonic interferons and of the main serum Proteins specific for Pregnancy
Placenta, 1992Co-Authors: Jacques Martal, Nicole CheneAbstract:Summary The nature and functions of the main Proteins involved in the control of Pregnancy are described with a particular emphasis on the embryonic signals, in the human species and domestic animals, necessary to the early Pregnancy recognition. A well characterized ovine antiluteolytic Protein (trophoblastin or oTP) maintains luteal function for a prolonged period despite a restricted period of expression. It also has interferon properties and its sequence homology is close to interferons α of class II (IFN w). In women, many Proteins are useful in the diagnosis of early Pregnancy and/or in abnormalities cases, for example chorionic gonadotropins (CG), “Schwangershafts Protein-1” (SP1), and early Pregnancy factor (EPF); or in ruminants the early Pregnancy Associated Protein (EPAP), and Pregnancy specific Proteins (PSP) which constitute a test of early Pregnancy which might substitute for the classical method based upon progesterone assay. These Proteins may also have luteotrophic, immunosuppressive, or enzymatic properties. Embryonic and fetal development depends upon placental lactogen (PL) or chorionic somatomammotropin (CS), and probably human Placental Growth Hormone (hPGH) acting as growth hormones. Some Proteins can bind substances (vitamin A, somatomedins, steroids, fatty acids…) involved in fetal growth, such as placental Proteins (PP12 and 14) and alpha-fetoProtein. The authors are grateful to Mrs Annick Bouroche for the translation of this text into English. They would like to thank Drs Sylvaine Camous and G. Charpigny, M. Guillomot, and K. Zouari for sharing with us their personal results.
Jean-louisbeaudeux - One of the best experts on this subject based on the ideXlab platform.
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Serum Plasma Pregnancy-Associated Protein A
Arteriosclerosis Thrombosis and Vascular Biology, 2003Co-Authors: Jean-louisbeaudeux, Laurenceburc, Françoiseimbert-bismut, Philippegiral, Maguybernard, Ericbruckert, M. JohnchapmanAbstract:Objective— The proteolytic activity of metalloProteinases, proinflammatory enzymes that degrade extracellular matrix, is elevated in lipid-rich atherosclerotic plaques, thereby contributing to plaque fragility and rupture. Pregnancy-Associated plasma Protein (PAPP-A) is a metalloProteinase, expressed in unstable atherosclerotic plaques, whose circulating levels are elevated in acute coronary syndromes. We evaluated serum PAPP-A levels as a marker of the premature development of atherosclerosis in hyperlipidemic subjects at elevated cardiovascular risk. Methods and Results— Serum PAPP-A levels were determined in asymptomatic hyperlipidemic male subjects (n=64; mean±SD age, 51±7 years) in whom intima-media thickness (IMT) and lesion status in the carotid artery were evaluated by noninvasive ultrasonography and compared with those of a normolipidemic control group (n=25). No difference was observed in circulating PAPP-A levels between hyperlipidemic subjects and controls (8.99±2.93 and 8.03±2.75 mIU/L, respe...
John M Chapman - One of the best experts on this subject based on the ideXlab platform.
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serum plasma Pregnancy Associated Protein a a potential marker of echogenic carotid atherosclerotic plaques in asymptomatic hyperlipidemic subjects at high cardiovascular risk
Arteriosclerosis Thrombosis and Vascular Biology, 2003Co-Authors: Jeanlouis Beaudeux, L Burc, Francoise Imbertbismut, Philippe Giral, Maguy Bernard, E Bruckert, John M ChapmanAbstract:Objective— The proteolytic activity of metalloProteinases, proinflammatory enzymes that degrade extracellular matrix, is elevated in lipid-rich atherosclerotic plaques, thereby contributing to plaque fragility and rupture. Pregnancy-Associated plasma Protein (PAPP-A) is a metalloProteinase, expressed in unstable atherosclerotic plaques, whose circulating levels are elevated in acute coronary syndromes. We evaluated serum PAPP-A levels as a marker of the premature development of atherosclerosis in hyperlipidemic subjects at elevated cardiovascular risk. Methods and Results— Serum PAPP-A levels were determined in asymptomatic hyperlipidemic male subjects (n=64; mean±SD age, 51±7 years) in whom intima-media thickness (IMT) and lesion status in the carotid artery were evaluated by noninvasive ultrasonography and compared with those of a normolipidemic control group (n=25). No difference was observed in circulating PAPP-A levels between hyperlipidemic subjects and controls (8.99±2.93 and 8.03±2.75 mIU/L, respe...