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Richard Auria - One of the best experts on this subject based on the ideXlab platform.

  • phenol biodegradation by the thermoacidophilic archaeon sulfolobus solfataricus 98 2 in a fed batch bioreactor
    Biodegradation, 2011
    Co-Authors: Pierre Christen, Sylvain Davidson, Yannick Combetblanc, Richard Auria
    Abstract:

    Toxic at low concentrations, phenol is one of the most common organic pollutants in air and water. In this work, phenol biodegradation was studied in extreme conditions (80°C, pH = 3.2) in a 2.7 l bioreactor with the thermoacidophilic archaeon Sulfolobus solfataricus 98/2. The strain was first acclimatized to phenol on a mixture of glucose (2000 mg l−1) and phenol (94 mg l−1) at a constant dissolved oxygen concentration of 1.5 mg l−1. After a short lag-phase, only glucose was consumed. Phenol degradation then began while glucose was still present in the reactor. When glucose was exhausted, phenol was used for respiration and then for biomass build-up. After several batch runs (phenol < 365 mg l−1), specific growth rate (μX) was 0.034 ± 0.001 h−1, specific phenol degradation rate (qP) was 57.5 ± 2 mg g−1 h−1, biomass yield (YX/P) was 52.2 ± 1.1 g mol−1, and oxygen yield factor \( \left( {{\text{Y}}_{{{\text{X}}/{\text{O}}_{ 2} }} } \right) \) was 9.2 ± 0.2 g mol−1. A carbon recovery close to 100% suggested that phenol was exclusively transformed into biomass (35%) and CO2 (65%). Molar phenol oxidation constant \( \left( {{\text{Y}}_{{{\text{O}}_{ 2} /{\text{P}}}} } \right) \) was calculated from stoichiometry of phenol oxidation and introducing experimental biomass and CO2 conversion yields on phenol, leading to values varying between 4.78 and 5.22 mol mol−1. Respiratory quotient was about 0.84 mol mol−1, very close to theoretical value (0.87 mol mol−1). Carbon dioxide production, oxygen demand and redox potential, monitored on-line, were good indicators of growth, substrate consumption and exhaustion, and can therefore be usefully employed for industrial phenol bioremediation in extreme environments.

Pierre Christen - One of the best experts on this subject based on the ideXlab platform.

  • phenol biodegradation by the thermoacidophilic archaeon sulfolobus solfataricus 98 2 in a fed batch bioreactor
    Biodegradation, 2011
    Co-Authors: Pierre Christen, Sylvain Davidson, Yannick Combetblanc, Richard Auria
    Abstract:

    Toxic at low concentrations, phenol is one of the most common organic pollutants in air and water. In this work, phenol biodegradation was studied in extreme conditions (80°C, pH = 3.2) in a 2.7 l bioreactor with the thermoacidophilic archaeon Sulfolobus solfataricus 98/2. The strain was first acclimatized to phenol on a mixture of glucose (2000 mg l−1) and phenol (94 mg l−1) at a constant dissolved oxygen concentration of 1.5 mg l−1. After a short lag-phase, only glucose was consumed. Phenol degradation then began while glucose was still present in the reactor. When glucose was exhausted, phenol was used for respiration and then for biomass build-up. After several batch runs (phenol < 365 mg l−1), specific growth rate (μX) was 0.034 ± 0.001 h−1, specific phenol degradation rate (qP) was 57.5 ± 2 mg g−1 h−1, biomass yield (YX/P) was 52.2 ± 1.1 g mol−1, and oxygen yield factor \( \left( {{\text{Y}}_{{{\text{X}}/{\text{O}}_{ 2} }} } \right) \) was 9.2 ± 0.2 g mol−1. A carbon recovery close to 100% suggested that phenol was exclusively transformed into biomass (35%) and CO2 (65%). Molar phenol oxidation constant \( \left( {{\text{Y}}_{{{\text{O}}_{ 2} /{\text{P}}}} } \right) \) was calculated from stoichiometry of phenol oxidation and introducing experimental biomass and CO2 conversion yields on phenol, leading to values varying between 4.78 and 5.22 mol mol−1. Respiratory quotient was about 0.84 mol mol−1, very close to theoretical value (0.87 mol mol−1). Carbon dioxide production, oxygen demand and redox potential, monitored on-line, were good indicators of growth, substrate consumption and exhaustion, and can therefore be usefully employed for industrial phenol bioremediation in extreme environments.

Yannick Combetblanc - One of the best experts on this subject based on the ideXlab platform.

  • phenol biodegradation by the thermoacidophilic archaeon sulfolobus solfataricus 98 2 in a fed batch bioreactor
    Biodegradation, 2011
    Co-Authors: Pierre Christen, Sylvain Davidson, Yannick Combetblanc, Richard Auria
    Abstract:

    Toxic at low concentrations, phenol is one of the most common organic pollutants in air and water. In this work, phenol biodegradation was studied in extreme conditions (80°C, pH = 3.2) in a 2.7 l bioreactor with the thermoacidophilic archaeon Sulfolobus solfataricus 98/2. The strain was first acclimatized to phenol on a mixture of glucose (2000 mg l−1) and phenol (94 mg l−1) at a constant dissolved oxygen concentration of 1.5 mg l−1. After a short lag-phase, only glucose was consumed. Phenol degradation then began while glucose was still present in the reactor. When glucose was exhausted, phenol was used for respiration and then for biomass build-up. After several batch runs (phenol < 365 mg l−1), specific growth rate (μX) was 0.034 ± 0.001 h−1, specific phenol degradation rate (qP) was 57.5 ± 2 mg g−1 h−1, biomass yield (YX/P) was 52.2 ± 1.1 g mol−1, and oxygen yield factor \( \left( {{\text{Y}}_{{{\text{X}}/{\text{O}}_{ 2} }} } \right) \) was 9.2 ± 0.2 g mol−1. A carbon recovery close to 100% suggested that phenol was exclusively transformed into biomass (35%) and CO2 (65%). Molar phenol oxidation constant \( \left( {{\text{Y}}_{{{\text{O}}_{ 2} /{\text{P}}}} } \right) \) was calculated from stoichiometry of phenol oxidation and introducing experimental biomass and CO2 conversion yields on phenol, leading to values varying between 4.78 and 5.22 mol mol−1. Respiratory quotient was about 0.84 mol mol−1, very close to theoretical value (0.87 mol mol−1). Carbon dioxide production, oxygen demand and redox potential, monitored on-line, were good indicators of growth, substrate consumption and exhaustion, and can therefore be usefully employed for industrial phenol bioremediation in extreme environments.

Sylvain Davidson - One of the best experts on this subject based on the ideXlab platform.

  • phenol biodegradation by the thermoacidophilic archaeon sulfolobus solfataricus 98 2 in a fed batch bioreactor
    Biodegradation, 2011
    Co-Authors: Pierre Christen, Sylvain Davidson, Yannick Combetblanc, Richard Auria
    Abstract:

    Toxic at low concentrations, phenol is one of the most common organic pollutants in air and water. In this work, phenol biodegradation was studied in extreme conditions (80°C, pH = 3.2) in a 2.7 l bioreactor with the thermoacidophilic archaeon Sulfolobus solfataricus 98/2. The strain was first acclimatized to phenol on a mixture of glucose (2000 mg l−1) and phenol (94 mg l−1) at a constant dissolved oxygen concentration of 1.5 mg l−1. After a short lag-phase, only glucose was consumed. Phenol degradation then began while glucose was still present in the reactor. When glucose was exhausted, phenol was used for respiration and then for biomass build-up. After several batch runs (phenol < 365 mg l−1), specific growth rate (μX) was 0.034 ± 0.001 h−1, specific phenol degradation rate (qP) was 57.5 ± 2 mg g−1 h−1, biomass yield (YX/P) was 52.2 ± 1.1 g mol−1, and oxygen yield factor \( \left( {{\text{Y}}_{{{\text{X}}/{\text{O}}_{ 2} }} } \right) \) was 9.2 ± 0.2 g mol−1. A carbon recovery close to 100% suggested that phenol was exclusively transformed into biomass (35%) and CO2 (65%). Molar phenol oxidation constant \( \left( {{\text{Y}}_{{{\text{O}}_{ 2} /{\text{P}}}} } \right) \) was calculated from stoichiometry of phenol oxidation and introducing experimental biomass and CO2 conversion yields on phenol, leading to values varying between 4.78 and 5.22 mol mol−1. Respiratory quotient was about 0.84 mol mol−1, very close to theoretical value (0.87 mol mol−1). Carbon dioxide production, oxygen demand and redox potential, monitored on-line, were good indicators of growth, substrate consumption and exhaustion, and can therefore be usefully employed for industrial phenol bioremediation in extreme environments.

Amal K Misra - One of the best experts on this subject based on the ideXlab platform.

  • removal of phenols from water environment by activated carbon bagasse ash and wood charcoal
    Chemical Engineering Journal, 2007
    Co-Authors: Somnath Mukherjee, Sunil Kumar, Amal K Misra, Maohong Fan
    Abstract:

    Abstract Adsorption process is gaining interest as one of the effective processes of advanced wastewater treatment for treatment of industrial effluent containing toxic materials. The present work involves an investigation of the use of three carbonaceous materials, activated carbon (AC), bagasse ash (BA) and wood charcoal (WC), as adsorbents for removal of phenol from water. Batch experiments were carried out to obtain adsorption equilibrium isotherms and kinetics with phenol spiked synthetic solutions. The study was performed with two initial phenol concentrations, viz. 30 and 50 mg/L, with an equal amount of adsorbent dose (50 g/L). The effects of solution pH, concentrations of EDTA, anions, and dosages of adsorbent on removal of phenol were examined. Desorption tests were also conducted in the present study. The suitability of the different isotherm models to the equilibrium data was studied for each phenol–adsorbent system. Experimental results showed that for phenol–AC, phenol–WC and phenol–BA adsorption systems, approximately 98%, 90% and 90% removal efficiencies were achieved at given adsorption conditions. The kinetic study indicates that the phenol removal with the selected adsorbents is a first order adsorption. Freundlich isotherm model was found to fit the data for adsorption of phenol with the adsorbents. Removal efficiency of phenol slightly increased when the pH of adsorption system decreased. The effect of nitrate ion and EDTA in the solution on the adsorption of phenol was found to be insignificant; however, the chloride ion has considerable negative effects on the removal by BA. The estimation of diffusion coefficients indicated that film diffusion may control the adsorption of phenol with the studied adsorbent materials.

  • removal of phenols from water environment by activated carbon bagasse ash and wood charcoal
    Chemical Engineering Journal, 2007
    Co-Authors: Somnath Mukherjee, Sunil Kumar, Amal K Misra
    Abstract:

    Abstract Adsorption process is gaining interest as one of the effective processes of advanced wastewater treatment for treatment of industrial effluent containing toxic materials. The present work involves an investigation of the use of three carbonaceous materials, activated carbon (AC), bagasse ash (BA) and wood charcoal (WC), as adsorbents for removal of phenol from water. Batch experiments were carried out to obtain adsorption equilibrium isotherms and kinetics with phenol spiked synthetic solutions. The study was performed with two initial phenol concentrations, viz. 30 and 50 mg/L, with an equal amount of adsorbent dose (50 g/L). The effects of solution pH, concentrations of EDTA, anions, and dosages of adsorbent on removal of phenol were examined. Desorption tests were also conducted in the present study. The suitability of the different isotherm models to the equilibrium data was studied for each phenol–adsorbent system. Experimental results showed that for phenol–AC, phenol–WC and phenol–BA adsorption systems, approximately 98%, 90% and 90% removal efficiencies were achieved at given adsorption conditions. The kinetic study indicates that the phenol removal with the selected adsorbents is a first order adsorption. Freundlich isotherm model was found to fit the data for adsorption of phenol with the adsorbents. Removal efficiency of phenol slightly increased when the pH of adsorption system decreased. The effect of nitrate ion and EDTA in the solution on the adsorption of phenol was found to be insignificant; however, the chloride ion has considerable negative effects on the removal by BA. The estimation of diffusion coefficients indicated that film diffusion may control the adsorption of phenol with the studied adsorbent materials.